Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Amidophosphoribosyltransferase

Gene

Ppat

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (Ppat), Amidophosphoribosyltransferase (Ppat), Amidophosphoribosyltransferase (Ppat), Amidophosphoribosyltransferase (Ppat)
  2. Trifunctional purine biosynthetic protein adenosine-3 (Gart), Trifunctional purine biosynthetic protein adenosine-3 (Gart)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12For GATase activityUniRule annotation1
Metal bindingi280Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi426Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi503Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi506Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotation, Transferase
Biological processPurine biosynthesisUniRule annotation
LigandIron, Iron-sulfurUniRule annotation, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:PpatImported
ORF Names:mCG_15865Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2387203. Ppat.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029246.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000120632.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 261Glutamine amidotransferase type-2InterPro annotationAdd BLAST250

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0572. Eukaryota.
COG0034. LUCA.
GeneTreeiENSGT00390000003428.
HOVERGENiHBG002589.
KOiK00764.
OMAiHVYFARP.
OrthoDBiEOG091G06F7.
TreeFamiTF106370.

Family and domain databases

CDDicd00715. GPATase_N. 1 hit.
cd06223. PRTases_typeI. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
IPR035584. PurF_N.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CIH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELEELGIRE ECGVFGCIAS GDWPTQLDVP HVITLGLVGL QHRGQESAGI
60 70 80 90 100
VTSDGSAVPK FRVHKGMGLV NHVFTEDNLK KLYDSNLGIG HTRYATTGKC
110 120 130 140 150
ELENCQPFVV ETLHGKIAVA HNGELVNAAR LRKKLLRQGI GLSTSSDSEM
160 170 180 190 200
ITQLLAYTPP QEKDDAPDWV ARIKNLMKEA PAAYSLVIMH RDFIYAVRDP
210 220 230 240 250
YGNRPLCIGR LMPVSDVNDK EKKSSETEGW VVSSESCSFL SIGARYCHEV
260 270 280 290 300
KPGEIVEISR HGIRTLDIIP RSNGDPVAFC IFEYVYFARP DSMFEDQMVY
310 320 330 340 350
TVRYRCGQQL AIEAPVEADL VSTVPESATP AALGYATKCG LPYVEVLCKN
360 370 380 390 400
RYVGRTFIQP NMRLRQLGVA KKFGVLSDNF KGKRIVLIDD SIVRGNTISP
410 420 430 440 450
IIKLLKESGA KEVHIRVASP PIKYPCFMGI NIPTKEELIA NKPEFDCLAE
460 470 480 490 500
YLGANSVVYL SVEGLVSSVQ QEIKFKKQKV KKHDIAIQEN GNGLEYFEKT
510
GHCTACLTGQ YPVELEW
Length:517
Mass (Da):57,403
Last modified:March 1, 2003 - v1
Checksum:i730F84E0B31A72BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC114666 Genomic DNA. No translation available.
BC023841 mRNA. Translation: AAH23841.1.
CH466524 Genomic DNA. Translation: EDL37915.1.
RefSeqiNP_742158.1. NM_172146.2.
UniGeneiMm.202337.

Genome annotation databases

EnsembliENSMUST00000140076; ENSMUSP00000120632; ENSMUSG00000029246.
GeneIDi231327.
KEGGimmu:231327.
UCSCiuc008xvl.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiQ8CIH9_MOUSE
AccessioniPrimary (citable) accession number: Q8CIH9
Entry historyiIntegrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: August 30, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported