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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Gene

Plcg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei327PROSITE-ProRule annotation1
Active sitei372PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • activation of store-operated calcium channel activity Source: MGI
  • B cell differentiation Source: UniProtKB
  • B cell receptor signaling pathway Source: UniProtKB
  • follicular B cell differentiation Source: MGI
  • inositol trisphosphate biosynthetic process Source: MGI
  • intracellular signal transduction Source: InterPro
  • negative regulation of programmed cell death Source: MGI
  • phosphatidylinositol biosynthetic process Source: UniProtKB
  • phospholipid catabolic process Source: InterPro
  • positive regulation of cytosolic calcium ion concentration Source: MGI
  • positive regulation of receptor internalization Source: CACAO
  • positive regulation of type I interferon production Source: CACAO
  • regulation of gene expression Source: MGI
  • release of sequestered calcium ion into cytosol Source: UniProtKB
  • response to ATP Source: MGI
  • response to lipopolysaccharide Source: MGI
  • response to magnesium ion Source: MGI
  • T cell receptor signaling pathway Source: MGI

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium

Enzyme and pathway databases

BRENDAi3.1.4.11 3474
ReactomeiR-MMU-114604 GPVI-mediated activation cascade
R-MMU-166016 Toll Like Receptor 4 (TLR4) Cascade
R-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol
R-MMU-2029485 Role of phospholipids in phagocytosis
R-MMU-2424491 DAP12 signaling
R-MMU-2871796 FCERI mediated MAPK activation
R-MMU-2871809 FCERI mediated Ca+2 mobilization
R-MMU-5607764 CLEC7A (Dectin-1) signaling
R-MMU-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-gamma-2
Phospholipase C-gamma-2
Short name:
PLC-gamma-2
Gene namesi
Name:Plcg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:97616 Plcg2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3608199

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003423641 – 12651-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2Add BLAST1265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei753Phosphotyrosine; by BTKCombined sources1
Modified residuei759Phosphotyrosine; by BTKBy similarity1
Modified residuei1197Phosphotyrosine; by BTKBy similarity1
Modified residuei1217PhosphotyrosineCombined sources1
Modified residuei1245PhosphotyrosineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity (By similarity). Phosphorylated on tyrosine residues by CSF1R.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CIH5
MaxQBiQ8CIH5
PaxDbiQ8CIH5
PeptideAtlasiQ8CIH5
PRIDEiQ8CIH5

PTM databases

iPTMnetiQ8CIH5
PhosphoSitePlusiQ8CIH5

Expressioni

Gene expression databases

BgeeiENSMUSG00000034330
GenevisibleiQ8CIH5 MM

Interactioni

Subunit structurei

Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231578, 2 interactors
CORUMiQ8CIH5
DIPiDIP-33368N
IntActiQ8CIH5, 33 interactors
MINTiQ8CIH5
STRINGi10090.ENSMUSP00000079991

Structurei

Secondary structure

11265
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi523 – 525Combined sources3
Helixi540 – 551Combined sources12
Turni552 – 555Combined sources4
Beta strandi561 – 565Combined sources5
Beta strandi567 – 569Combined sources3
Beta strandi573 – 579Combined sources7
Beta strandi582 – 593Combined sources12
Beta strandi596 – 602Combined sources7
Beta strandi607 – 609Combined sources3
Helixi610 – 616Combined sources7
Turni617 – 619Combined sources3
Helixi625 – 627Combined sources3
Beta strandi773 – 778Combined sources6
Beta strandi784 – 786Combined sources3
Beta strandi795 – 798Combined sources4
Beta strandi803 – 805Combined sources3
Beta strandi807 – 811Combined sources5
Beta strandi814 – 819Combined sources6
Helixi821 – 823Combined sources3
Beta strandi824 – 826Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DX0X-ray2.50A/B516-640[»]
2EQINMR-A772-827[»]
ProteinModelPortaliQ8CIH5
SMRiQ8CIH5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CIH5

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 131PHPROSITE-ProRule annotationAdd BLAST131
Domaini312 – 456PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST145
Domaini532 – 635SH2 1PROSITE-ProRule annotationAdd BLAST104
Domaini646 – 735SH2 2PROSITE-ProRule annotationAdd BLAST90
Domaini769 – 829SH3PROSITE-ProRule annotationAdd BLAST61
Domaini930 – 1044PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST115
Domaini1048 – 1152C2PROSITE-ProRule annotationAdd BLAST105

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG1264 Eukaryota
ENOG410XPXE LUCA
GeneTreeiENSGT00730000110782
HOGENOMiHOG000230864
HOVERGENiHBG053611
InParanoidiQ8CIH5
KOiK05859
OMAiMLMRIPR
OrthoDBiEOG091G07R3
PhylomeDBiQ8CIH5
TreeFamiTF313216

Family and domain databases

CDDicd09932 SH2_C-SH2_PLC_gamma_like, 1 hit
cd10341 SH2_N-SH2_PLC_gamma_like, 1 hit
cd11969 SH3_PLCgamma2, 1 hit
Gene3Di2.30.29.30, 2 hits
2.60.40.150, 1 hit
3.20.20.190, 2 hits
3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001192 PI-PLC_fam
IPR016279 PLC-gamma
IPR028381 PLC-gamma2
IPR035023 PLC-gamma_C-SH2
IPR035024 PLC-gamma_N-SH2
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR035723 PLCgamma2_SH3
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF25 PTHR10336:SF25, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF00017 SH2, 2 hits
PF00018 SH3_1, 1 hit
PIRSFiPIRSF000952 PLC-gamma, 1 hit
PRINTSiPR00390 PHPHLIPASEC
PR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00233 PH, 2 hits
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SM00252 SH2, 2 hits
SM00326 SH3, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF50044 SSF50044, 1 hit
SSF51695 SSF51695, 2 hits
SSF55550 SSF55550, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit
PS50001 SH2, 2 hits
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CIH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMVNVDTL PEYEKSQIKR ALELGTVMTV FNARKSTPER RTVQMIMETR
60 70 80 90 100
QVAWSKTADK IEGFLDIMEI KEIRPGKNSK DFERAKAVRH KAECCFTILY
110 120 130 140 150
GTQFVLSTLS LATDSKEDAV KWLSGLKILH QEAMSASTPT MIESWLRKQI
160 170 180 190 200
YSVDQTRRNS ISLRELKTIL PLVNFKVSGI KFLKDKLVEI GAQKDELSFE
210 220 230 240 250
QFHLFYKKLM FDQQKSILDE FKKDSSVFIL GNTDRPDASA VYLQDFQRFL
260 270 280 290 300
LHEQQELWAQ DLNKVRERMT KFIDDTMRET AEPFLFVDEF LTYLFSRENS
310 320 330 340 350
IWDEKYDAVD MQDMNNPLSH YWISSSHNTY LTGDQLRSES STEAYIRCLR
360 370 380 390 400
AGCRCIELDC WDGPDGKPII YHGWTRTTKI KFDDVVQAIR DHAFVTSSFP
410 420 430 440 450
VILSIEEHCS VEQQRHMAKV FKEVLGDMLL TKPTEASADQ LPSPSQLREK
460 470 480 490 500
IIIKHKKLGP KGDVDVNVED KKDEHKPQGE LYMWDSIDQK WTRHYCAIAD
510 520 530 540 550
AKLSFGDDIE QAVEEEPVQD TPPTELHFGE KWFHKKVESR TSAEKLLQEY
560 570 580 590 600
CAETGAKDGT FLVRESETFP NDYTLSFWRS GRVQHCRIRS TMENGVMKYY
610 620 630 640 650
LTDNLTFNSI YALIQHYREA HLRCAEFELR LTDPVPNPNP HESKPWYYDS
660 670 680 690 700
LSRGEAEDML MRIPRDGAFL IRKREGTNSY AITFRARGKV KHCRINRDGR
710 720 730 740 750
HFVLGTSAYF ESLVELVSYY EKHALYRKMR LRYPVTPELL ERYNMERDIN
760 770 780 790 800
SLYDVSRMYV DPSEINPSMP QRTVKALYDY KAKRSDELTF CRGALIHNVS
810 820 830 840 850
KEPGGWWKGD YGTRIQQYFP SNYVEDISAG DAEEMEKQII EDNPLGSLCK
860 870 880 890 900
GILDLNTYNV VKAPQGKNQK AFVFILEPKK QGDPPVEFAT DRVEELFEWF
910 920 930 940 950
QSIREITWKM DTKENNMKYW ERNQSIAIEL SDLVVYCKPT SKTKDHLENP
960 970 980 990 1000
DFREIRSFVE TKADSIVRQK PVDLLRYNQK GLTRVYPKGQ RVDSSNYDPF
1010 1020 1030 1040 1050
RLWLCGSQMV ALNFQTPDKY MQMNHALFSL NGRTGYVLQP ESMRSEKYDP
1060 1070 1080 1090 1100
MPLESQRKIL MTLTVKVLGA RHLPKLGRSI ACPFVEVEIC GAEYDSNKFK
1110 1120 1130 1140 1150
TTVVNDNGLS PVWAPTQEKV TFEIYDPNLA FLRFVVYEED MFSDPNFLAH
1160 1170 1180 1190 1200
ATYPIKGIKS GFRSVPLKNG YSEDIELASL LVFCEMRPVL ESEEELYSSC
1210 1220 1230 1240 1250
RQLRRRQEEL NNQLFLYDTH QNLRGANRDA LVKEFNVNEN QLQLYQEKCN
1260
RRLREKRVSN SRFYS
Length:1,265
Mass (Da):147,592
Last modified:March 1, 2003 - v1
Checksum:iE4A2A6AD6F05160E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti787E → G in BAE24959 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK142173 mRNA Translation: BAE24959.1
AK151039 mRNA Translation: BAE30056.1
BC019654 mRNA Translation: AAH19654.1
BC023877 mRNA Translation: AAH23877.1
CCDSiCCDS22700.1
RefSeqiNP_758489.1, NM_172285.2
UniGeneiMm.192699

Genome annotation databases

EnsembliENSMUST00000081232; ENSMUSP00000079991; ENSMUSG00000034330
GeneIDi234779
KEGGimmu:234779
UCSCiuc009npc.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPLCG2_MOUSE
AccessioniPrimary (citable) accession number: Q8CIH5
Secondary accession number(s): Q3UBA8, Q3UQT0, Q8VE69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: March 1, 2003
Last modified: June 20, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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