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Protein

DEP domain-containing protein 1A

Gene

Depdc1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DEP domain-containing protein 1A
Gene namesi
Name:Depdc1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1923381. Depdc1a.

Subcellular locationi

  • Nucleus By similarity

  • Note: Colocalizes with ZNF224 at the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804DEP domain-containing protein 1APRO_0000284787Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei513 – 5131PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CIG0.
PRIDEiQ8CIG0.

PTM databases

iPTMnetiQ8CIG0.
PhosphoSiteiQ8CIG0.

Expressioni

Gene expression databases

BgeeiQ8CIG0.
CleanExiMM_DEPDC1A.
ExpressionAtlasiQ8CIG0. baseline and differential.
GenevisibleiQ8CIG0. MM.

Interactioni

Subunit structurei

Can form dimers. Interacts with ZNF224 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113216.

Structurei

3D structure databases

ProteinModelPortaliQ8CIG0.
SMRiQ8CIG0. Positions 11-108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 10885DEPPROSITE-ProRule annotationAdd
BLAST
Domaini282 – 32241Rho-GAPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni592 – 64756Interaction with ZNF224By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili726 – 76035Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEI5. Eukaryota.
ENOG410YMM7. LUCA.
GeneTreeiENSGT00530000063030.
HOGENOMiHOG000013064.
HOVERGENiHBG059792.
InParanoidiQ8CIG0.
PhylomeDBiQ8CIG0.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.555.10. 2 hits.
InterProiIPR000591. DEP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48350. SSF48350. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CIG0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERQGAPLGP YRATKLWNEV TTSFRVGMPL RKHRQHLKKY SNCFTAVEAI
60 70 80 90 100
DWLYDLLRSN SNFGPEVTRQ QTIQLLRKFL KNHVIEDIKG RWGSESLDDN
110 120 130 140 150
NQLFRFPATS PLKTFPQRHT EIKKTNTEYF SKDKDGIFKL RNLSQKTSKK
160 170 180 190 200
RGLHFSQENT EKINHERITN EDLEIAPDNQ EISQEDVEEV WRYVIMIYLQ
210 220 230 240 250
TILSLPSIEE LLNPNQVIPQ YIMYNMANTS KHGVVILQDK SDDLPHWVLS
260 270 280 290 300
AMKCLANWPR SNDTNNLTYV GFERDVFKTI ADYFLNLPEP LLTFEYYELF
310 320 330 340 350
VNILVVCGYI TVSDRTSGIH KIQDDPRSSK IHDLSNLNSF KSTECLLLSL
360 370 380 390 400
LYKDKSNEEL DSTKRLQRND QGFQERCAKK MQLDNLRNRR ASANDIMGGS
410 420 430 440 450
CHNLIGLSNT NALSSNIKPR CSSLEGIVDR PVNSSEKKSS IFYQSVLNIE
460 470 480 490 500
EQNSKQSLVS APKQTPLFNL HSDENAQQPH CVGFNRTSAL TVQDQEELCN
510 520 530 540 550
EKYKSKQLCR SQSLLLRSST RQNSCINKPV AEIIMKPNVG QGSTSELGES
560 570 580 590 600
STTINKRLCK STIELSEKSL PPAASVLTGT QSLLQPHLER VAINALQLCC
610 620 630 640 650
LLLPPPNRRK LQLLMRMISR MSQNVDMPKL HEQIGTRSLM INTFSRCVLC
660 670 680 690 700
CAEEVDLDEL LASRLVSFLM DHHQEILQVP TYLQAAVEKH LDYIKKGNVK
710 720 730 740 750
NHGDGLVVPL PTYSYCKQIS AKEFDEQKIS TSQAAIAELL ENIVRSKSLS
760 770 780 790 800
LKEKRRKLKQ KEYPLIYQKR FPTTESEAAL FDDKPTIKQP MLNLRNPKLH

SLRY
Length:804
Mass (Da):92,201
Last modified:March 1, 2003 - v1
Checksum:iC811F34A87EE33D9
GO
Isoform 2 (identifier: Q8CIG0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     305-305: V → G
     306-582: Missing.
     760-760: Q → QFQ

Note: No experimental confirmation available.
Show »
Length:529
Mass (Da):61,785
Checksum:i257B6657E242D591
GO

Sequence cautioni

The sequence AAH05799.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei305 – 3051V → G in isoform 2. 2 PublicationsVSP_024656
Alternative sequencei306 – 582277Missing in isoform 2. 2 PublicationsVSP_024657Add
BLAST
Alternative sequencei760 – 7601Q → QFQ in isoform 2. 2 PublicationsVSP_024658

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050456 mRNA. Translation: BAC34267.1.
AK088719 mRNA. Translation: BAC40526.1.
BC005799 mRNA. Translation: AAH05799.1. Different initiation.
BC023934 mRNA. Translation: AAH23934.1.
CCDSiCCDS17936.1. [Q8CIG0-1]
CCDS51106.1. [Q8CIG0-2]
RefSeqiNP_001165563.1. NM_001172092.1.
NP_001165564.1. NM_001172093.1. [Q8CIG0-2]
NP_083799.2. NM_029523.3. [Q8CIG0-1]
UniGeneiMm.371655.

Genome annotation databases

EnsembliENSMUST00000029825; ENSMUSP00000029825; ENSMUSG00000028175. [Q8CIG0-1]
ENSMUST00000106041; ENSMUSP00000101656; ENSMUSG00000028175. [Q8CIG0-2]
GeneIDi76131.
KEGGimmu:76131.
UCSCiuc008rvy.2. mouse. [Q8CIG0-1]
uc008rwa.2. mouse. [Q8CIG0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK050456 mRNA. Translation: BAC34267.1.
AK088719 mRNA. Translation: BAC40526.1.
BC005799 mRNA. Translation: AAH05799.1. Different initiation.
BC023934 mRNA. Translation: AAH23934.1.
CCDSiCCDS17936.1. [Q8CIG0-1]
CCDS51106.1. [Q8CIG0-2]
RefSeqiNP_001165563.1. NM_001172092.1.
NP_001165564.1. NM_001172093.1. [Q8CIG0-2]
NP_083799.2. NM_029523.3. [Q8CIG0-1]
UniGeneiMm.371655.

3D structure databases

ProteinModelPortaliQ8CIG0.
SMRiQ8CIG0. Positions 11-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113216.

PTM databases

iPTMnetiQ8CIG0.
PhosphoSiteiQ8CIG0.

Proteomic databases

PaxDbiQ8CIG0.
PRIDEiQ8CIG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029825; ENSMUSP00000029825; ENSMUSG00000028175. [Q8CIG0-1]
ENSMUST00000106041; ENSMUSP00000101656; ENSMUSG00000028175. [Q8CIG0-2]
GeneIDi76131.
KEGGimmu:76131.
UCSCiuc008rvy.2. mouse. [Q8CIG0-1]
uc008rwa.2. mouse. [Q8CIG0-2]

Organism-specific databases

CTDi76131.
MGIiMGI:1923381. Depdc1a.

Phylogenomic databases

eggNOGiENOG410IEI5. Eukaryota.
ENOG410YMM7. LUCA.
GeneTreeiENSGT00530000063030.
HOGENOMiHOG000013064.
HOVERGENiHBG059792.
InParanoidiQ8CIG0.
PhylomeDBiQ8CIG0.

Miscellaneous databases

PROiQ8CIG0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CIG0.
CleanExiMM_DEPDC1A.
ExpressionAtlasiQ8CIG0. baseline and differential.
GenevisibleiQ8CIG0. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.555.10. 2 hits.
InterProiIPR000591. DEP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48350. SSF48350. 2 hits.
PROSITEiPS50186. DEP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J and NOD.
    Tissue: Liver and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 532-804 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 354-364, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiDEP1A_MOUSE
AccessioniPrimary (citable) accession number: Q8CIG0
Secondary accession number(s): Q8BHF1, Q99JN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.