Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8CIE6

- COPA_MOUSE

UniProt

Q8CIE6 - COPA_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Coatomer subunit alpha

Gene

Copa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).By similarity
Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor (By similarity).By similarity

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. intracellular protein transport Source: InterPro
  2. pancreatic juice secretion Source: Ensembl
  3. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_227904. COPI Mediated Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Coatomer subunit alpha
Alternative name(s):
Alpha-coat protein
Short name:
Alpha-COP
Cleaved into the following 2 chains:
Alternative name(s):
Xenopsin-related peptide
Gene namesi
Name:Copa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1334462. Copa.

Subcellular locationi

Cytoplasm By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicleCOPI-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.By similarity
Peptide Xenin : Secreted By similarity

GO - Cellular componenti

  1. COPI vesicle coat Source: InterPro
  2. extracellular space Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12241224Coatomer subunit alphaPRO_0000327496Add
BLAST
Peptidei1 – 3535ProxeninPRO_0000327497Add
BLAST
Peptidei1 – 2525XeninPRO_0000327498Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731PhosphoserineBy similarity
Modified residuei185 – 1851PhosphothreonineBy similarity
Modified residuei402 – 4021PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CIE6.
PaxDbiQ8CIE6.
PRIDEiQ8CIE6.

PTM databases

PhosphoSiteiQ8CIE6.

Expressioni

Gene expression databases

BgeeiQ8CIE6.
ExpressionAtlasiQ8CIE6. baseline and differential.
GenevestigatoriQ8CIE6.

Interactioni

Subunit structurei

Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1. Interacts with JAGN1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi198835. 20 interactions.
IntActiQ8CIE6. 19 interactions.
MINTiMINT-4116336.

Structurei

3D structure databases

ProteinModelPortaliQ8CIE6.
SMRiQ8CIE6. Positions 1-810, 905-1224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati7 – 3731WD 1Add
BLAST
Repeati49 – 7931WD 2Add
BLAST
Repeati91 – 12131WD 3Add
BLAST
Repeati133 – 16331WD 4Add
BLAST
Repeati203 – 23331WD 5Add
BLAST
Repeati247 – 27731WD 6Add
BLAST

Sequence similaritiesi

Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00520000055597.
HOGENOMiHOG000195913.
HOVERGENiHBG005379.
InParanoidiQ8CIE6.
KOiK05236.
OrthoDBiEOG78WKQX.
TreeFamiTF105693.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF51004. SSF51004. 3 hits.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CIE6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH
60 70 80 90 100
DGPVRGIDFH KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF
110 120 130 140 150
FHHEYPWILS ASDDQTIRVW NWQSRTCVCV LTGHNHYVMC AQFHPSEDLV
160 170 180 190 200
VSASLDQTVR VWDISGLRKK NLSPGAVESD VRGITGVDLF GTTDAVVKHV
210 220 230 240 250
LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW EVDTCRGHYN
260 270 280 290 300
NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH
310 320 330 340 350
PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK
360 370 380 390 400
DVAVMQLRSG SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA
410 420 430 440 450
DSQNPDAPEG KRSSGLTAVW VARNRFAVLD RMHSLLIKNL KNEITKKIQV
460 470 480 490 500
PNCDEIFYAG TGNLLLRDAD SITLFDVQQK RTLASVKISK VKYVIWSADM
510 520 530 540 550
SHVALLAKHA IVICNRKLDA LCNIHENIRV KSGAWDESGV FIYTTSNHIK
560 570 580 590 600
YAVTTGDHGI IRTLDLPIYV TRVKGNNVYC LDRECRPRVL TIDPTEFKFK
610 620 630 640 650
LALINRKYDE VLHMVRNAKL VGQSIIAYLQ KKGYPEVALH FVKDEKTRFS
660 670 680 690 700
LALECGNIEI ALEAAKALDD KNCWEKLGEV ALLQGNHQIV EMCYQRTKNF
710 720 730 740 750
DKLSFLYLIT GNLEKLRKMM KIAEIRKDMS GHYQNALYLG DVSERVRILK
760 770 780 790 800
NCGQKSLAYL SAATHGLDEE AESLKETFDP EKETIPDIDP NAKLLQPPAP
810 820 830 840 850
IMPLDTNWPL LTVSKGFFEG SIASKGKGGA LAADIDIDTV GTEGWGEDAE
860 870 880 890 900
LQLDEDGFVE APEGLGEDVL GKGQEEGGGW DVEEDLELPP ELDVPSGVSG
910 920 930 940 950
SAEDGFFVPP TKGTSPTQIW CNNSQLPVDH ILAGSFETAM RLLHDQVGVI
960 970 980 990 1000
QFGPYKQLFL QTYARGRTTY QALPCLPSMY SYPNRNWKDA GLKNGVPAVG
1010 1020 1030 1040 1050
LKLNDLIQRL QLCYQLTTVG KFEEAVEKFR SILLSVPLLV VDNKQEIAEA
1060 1070 1080 1090 1100
QQLITICREY IVGLCMEIER KKLPKETLDQ QKRICEMAAY FTHSNLQPVH
1110 1120 1130 1140 1150
MILVLRTALN LFFKLKNFKT AATFARRLLE LGPKPEVAQQ TRKILSACEK
1160 1170 1180 1190 1200
NPTDACQLNY DMHNPFDICA ASYRPIYRGK PVEKCPLSGA CYSPEFKGQI
1210 1220
CRVTTVTEIG KDVIGLRISP LQFR
Length:1,224
Mass (Da):138,432
Last modified:July 27, 2011 - v2
Checksum:i48AB72AD9CBDDD6B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti761 – 7611S → T in AAH24070. (PubMed:15489334)Curated
Sequence conflicti761 – 7611S → T in AAH47429. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC158930 Genomic DNA. No translation available.
BC024070 mRNA. Translation: AAH24070.1.
BC047429 mRNA. Translation: AAH47429.1.
CCDSiCCDS35780.1.
RefSeqiNP_034068.3. NM_009938.4.
UniGeneiMm.30041.
Mm.412417.

Genome annotation databases

EnsembliENSMUST00000135192; ENSMUSP00000118179; ENSMUSG00000026553.
GeneIDi12847.
KEGGimmu:12847.
UCSCiuc007dpn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC158930 Genomic DNA. No translation available.
BC024070 mRNA. Translation: AAH24070.1 .
BC047429 mRNA. Translation: AAH47429.1 .
CCDSi CCDS35780.1.
RefSeqi NP_034068.3. NM_009938.4.
UniGenei Mm.30041.
Mm.412417.

3D structure databases

ProteinModelPortali Q8CIE6.
SMRi Q8CIE6. Positions 1-810, 905-1224.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198835. 20 interactions.
IntActi Q8CIE6. 19 interactions.
MINTi MINT-4116336.

PTM databases

PhosphoSitei Q8CIE6.

Proteomic databases

MaxQBi Q8CIE6.
PaxDbi Q8CIE6.
PRIDEi Q8CIE6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000135192 ; ENSMUSP00000118179 ; ENSMUSG00000026553 .
GeneIDi 12847.
KEGGi mmu:12847.
UCSCi uc007dpn.2. mouse.

Organism-specific databases

CTDi 1314.
MGIi MGI:1334462. Copa.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00520000055597.
HOGENOMi HOG000195913.
HOVERGENi HBG005379.
InParanoidi Q8CIE6.
KOi K05236.
OrthoDBi EOG78WKQX.
TreeFami TF105693.

Enzyme and pathway databases

Reactomei REACT_227904. COPI Mediated Transport.

Miscellaneous databases

ChiTaRSi COPA. mouse.
NextBioi 282392.
PROi Q8CIE6.
SOURCEi Search...

Gene expression databases

Bgeei Q8CIE6.
ExpressionAtlasi Q8CIE6. baseline and differential.
Genevestigatori Q8CIE6.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR016391. Coatomer_asu.
IPR010714. Coatomer_asu_C.
IPR006692. Coatomer_WD-assoc_reg.
IPR020472. G-protein_beta_WD-40_rep.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF04053. Coatomer_WDAD. 1 hit.
PF06957. COPI_C. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view ]
PIRSFi PIRSF003354. Coatomer_alpha_subunit. 1 hit.
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 2 hits.
SSF51004. SSF51004. 3 hits.
PROSITEi PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver and Mammary tumor.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiCOPA_MOUSE
AccessioniPrimary (citable) accession number: Q8CIE6
Secondary accession number(s): E9Q075
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3