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Q8CIE6

- COPA_MOUSE

UniProt

Q8CIE6 - COPA_MOUSE

Protein

Coatomer subunit alpha

Gene

Copa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors By similarity.By similarity
    Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor By similarity.By similarity

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. intracellular protein transport Source: InterPro
    2. pancreatic juice secretion Source: Ensembl
    3. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hormone

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_227904. COPI Mediated Transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coatomer subunit alpha
    Alternative name(s):
    Alpha-coat protein
    Short name:
    Alpha-COP
    Cleaved into the following 2 chains:
    Alternative name(s):
    Xenopsin-related peptide
    Gene namesi
    Name:Copa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1334462. Copa.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicleCOPI-coated vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.By similarity
    Peptide Xenin : Secreted By similarity

    GO - Cellular componenti

    1. COPI vesicle coat Source: InterPro
    2. extracellular space Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12241224Coatomer subunit alphaPRO_0000327496Add
    BLAST
    Peptidei1 – 3535ProxeninPRO_0000327497Add
    BLAST
    Peptidei1 – 2525XeninPRO_0000327498Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei173 – 1731PhosphoserineBy similarity
    Modified residuei185 – 1851PhosphothreonineBy similarity
    Modified residuei402 – 4021PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8CIE6.
    PaxDbiQ8CIE6.
    PRIDEiQ8CIE6.

    PTM databases

    PhosphoSiteiQ8CIE6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CIE6.
    BgeeiQ8CIE6.
    GenevestigatoriQ8CIE6.

    Interactioni

    Subunit structurei

    Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198835. 20 interactions.
    IntActiQ8CIE6. 19 interactions.
    MINTiMINT-4116336.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CIE6.
    SMRiQ8CIE6. Positions 1-810, 905-1224.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati7 – 3731WD 1Add
    BLAST
    Repeati49 – 7931WD 2Add
    BLAST
    Repeati91 – 12131WD 3Add
    BLAST
    Repeati133 – 16331WD 4Add
    BLAST
    Repeati203 – 23331WD 5Add
    BLAST
    Repeati247 – 27731WD 6Add
    BLAST

    Sequence similaritiesi

    Contains 6 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00520000055597.
    HOGENOMiHOG000195913.
    HOVERGENiHBG005379.
    InParanoidiQ8CIE6.
    KOiK05236.
    OrthoDBiEOG78WKQX.
    TreeFamiTF105693.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR016391. Coatomer_asu.
    IPR010714. Coatomer_asu_C.
    IPR006692. Coatomer_WD-assoc_reg.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR011048. Haem_d1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF04053. Coatomer_WDAD. 1 hit.
    PF06957. COPI_C. 1 hit.
    PF00400. WD40. 6 hits.
    [Graphical view]
    PIRSFiPIRSF003354. Coatomer_alpha_subunit. 1 hit.
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    SSF51004. SSF51004. 3 hits.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CIE6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTKFETKSA RVKGLSFHPK RPWILTSLHN GVIQLWDYRM CTLIDKFDEH     50
    DGPVRGIDFH KQQPLFVSGG DDYKIKVWNY KLRRCLFTLL GHLDYIRTTF 100
    FHHEYPWILS ASDDQTIRVW NWQSRTCVCV LTGHNHYVMC AQFHPSEDLV 150
    VSASLDQTVR VWDISGLRKK NLSPGAVESD VRGITGVDLF GTTDAVVKHV 200
    LEGHDRGVNW AAFHPTMPLI VSGADDRQVK IWRMNESKAW EVDTCRGHYN 250
    NVSCAVFHPR QELILSNSED KSIRVWDMSK RTGVQTFRRD HDRFWVLAAH 300
    PNLNLFAAGH DGGMIVFKLE RERPAYAVHG NMLHYVKDRF LRQLDFNSSK 350
    DVAVMQLRSG SKFPVFNMSY NPAENAVLLC TRASNLENST YDLYTIPKDA 400
    DSQNPDAPEG KRSSGLTAVW VARNRFAVLD RMHSLLIKNL KNEITKKIQV 450
    PNCDEIFYAG TGNLLLRDAD SITLFDVQQK RTLASVKISK VKYVIWSADM 500
    SHVALLAKHA IVICNRKLDA LCNIHENIRV KSGAWDESGV FIYTTSNHIK 550
    YAVTTGDHGI IRTLDLPIYV TRVKGNNVYC LDRECRPRVL TIDPTEFKFK 600
    LALINRKYDE VLHMVRNAKL VGQSIIAYLQ KKGYPEVALH FVKDEKTRFS 650
    LALECGNIEI ALEAAKALDD KNCWEKLGEV ALLQGNHQIV EMCYQRTKNF 700
    DKLSFLYLIT GNLEKLRKMM KIAEIRKDMS GHYQNALYLG DVSERVRILK 750
    NCGQKSLAYL SAATHGLDEE AESLKETFDP EKETIPDIDP NAKLLQPPAP 800
    IMPLDTNWPL LTVSKGFFEG SIASKGKGGA LAADIDIDTV GTEGWGEDAE 850
    LQLDEDGFVE APEGLGEDVL GKGQEEGGGW DVEEDLELPP ELDVPSGVSG 900
    SAEDGFFVPP TKGTSPTQIW CNNSQLPVDH ILAGSFETAM RLLHDQVGVI 950
    QFGPYKQLFL QTYARGRTTY QALPCLPSMY SYPNRNWKDA GLKNGVPAVG 1000
    LKLNDLIQRL QLCYQLTTVG KFEEAVEKFR SILLSVPLLV VDNKQEIAEA 1050
    QQLITICREY IVGLCMEIER KKLPKETLDQ QKRICEMAAY FTHSNLQPVH 1100
    MILVLRTALN LFFKLKNFKT AATFARRLLE LGPKPEVAQQ TRKILSACEK 1150
    NPTDACQLNY DMHNPFDICA ASYRPIYRGK PVEKCPLSGA CYSPEFKGQI 1200
    CRVTTVTEIG KDVIGLRISP LQFR 1224
    Length:1,224
    Mass (Da):138,432
    Last modified:July 27, 2011 - v2
    Checksum:i48AB72AD9CBDDD6B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti761 – 7611S → T in AAH24070. (PubMed:15489334)Curated
    Sequence conflicti761 – 7611S → T in AAH47429. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC158930 Genomic DNA. No translation available.
    BC024070 mRNA. Translation: AAH24070.1.
    BC047429 mRNA. Translation: AAH47429.1.
    CCDSiCCDS35780.1.
    RefSeqiNP_034068.3. NM_009938.4.
    UniGeneiMm.30041.
    Mm.412417.

    Genome annotation databases

    EnsembliENSMUST00000135192; ENSMUSP00000118179; ENSMUSG00000026553.
    GeneIDi12847.
    KEGGimmu:12847.
    UCSCiuc007dpn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC158930 Genomic DNA. No translation available.
    BC024070 mRNA. Translation: AAH24070.1 .
    BC047429 mRNA. Translation: AAH47429.1 .
    CCDSi CCDS35780.1.
    RefSeqi NP_034068.3. NM_009938.4.
    UniGenei Mm.30041.
    Mm.412417.

    3D structure databases

    ProteinModelPortali Q8CIE6.
    SMRi Q8CIE6. Positions 1-810, 905-1224.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198835. 20 interactions.
    IntActi Q8CIE6. 19 interactions.
    MINTi MINT-4116336.

    PTM databases

    PhosphoSitei Q8CIE6.

    Proteomic databases

    MaxQBi Q8CIE6.
    PaxDbi Q8CIE6.
    PRIDEi Q8CIE6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000135192 ; ENSMUSP00000118179 ; ENSMUSG00000026553 .
    GeneIDi 12847.
    KEGGi mmu:12847.
    UCSCi uc007dpn.2. mouse.

    Organism-specific databases

    CTDi 1314.
    MGIi MGI:1334462. Copa.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00520000055597.
    HOGENOMi HOG000195913.
    HOVERGENi HBG005379.
    InParanoidi Q8CIE6.
    KOi K05236.
    OrthoDBi EOG78WKQX.
    TreeFami TF105693.

    Enzyme and pathway databases

    Reactomei REACT_227904. COPI Mediated Transport.

    Miscellaneous databases

    ChiTaRSi COPA. mouse.
    NextBioi 282392.
    PROi Q8CIE6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CIE6.
    Bgeei Q8CIE6.
    Genevestigatori Q8CIE6.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR016391. Coatomer_asu.
    IPR010714. Coatomer_asu_C.
    IPR006692. Coatomer_WD-assoc_reg.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR011048. Haem_d1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF04053. Coatomer_WDAD. 1 hit.
    PF06957. COPI_C. 1 hit.
    PF00400. WD40. 6 hits.
    [Graphical view ]
    PIRSFi PIRSF003354. Coatomer_alpha_subunit. 1 hit.
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    SSF51004. SSF51004. 3 hits.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 6 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver and Mammary tumor.
    3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiCOPA_MOUSE
    AccessioniPrimary (citable) accession number: Q8CIE6
    Secondary accession number(s): E9Q075
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3