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Q8CI94

- PYGB_MOUSE

UniProt

Q8CI94 - PYGB_MOUSE

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Protein

Glycogen phosphorylase, brain form

Gene

Pygb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity).By similarity

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

Enzyme regulationi

Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761AMPBy similarity
Sitei109 – 1091Involved in the association of subunitsBy similarity
Sitei143 – 1431Involved in the association of subunitsBy similarity
Sitei156 – 1561May be involved in allosteric controlBy similarity

GO - Molecular functioni

  1. glycogen phosphorylase activity Source: InterPro
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glycogen metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_256123. Glycogen breakdown (glycogenolysis).

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase, brain form (EC:2.4.1.1)
Gene namesi
Name:Pygb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:97828. Pygb.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 843842Glycogen phosphorylase, brain formPRO_0000188536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei15 – 151Phosphoserine; by PHK; in form phosphorylase ABy similarity
Modified residuei197 – 1971Phosphotyrosine1 Publication
Modified residuei473 – 4731Phosphotyrosine1 Publication
Modified residuei681 – 6811N6-(pyridoxal phosphate)lysineBy similarity

Post-translational modificationi

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8CI94.
PaxDbiQ8CI94.
PRIDEiQ8CI94.

PTM databases

PhosphoSiteiQ8CI94.

Expressioni

Gene expression databases

BgeeiQ8CI94.
CleanExiMM_PYGB.
ExpressionAtlasiQ8CI94. baseline and differential.
GenevestigatoriQ8CI94.

Interactioni

Subunit structurei

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A (By similarity).By similarity

Protein-protein interaction databases

BioGridi225278. 1 interaction.
IntActiQ8CI94. 4 interactions.
MINTiMINT-1870113.

Structurei

3D structure databases

ProteinModelPortaliQ8CI94.
SMRiQ8CI94. Positions 14-839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiCOG0058.
GeneTreeiENSGT00390000016886.
HOGENOMiHOG000278444.
HOVERGENiHBG006848.
InParanoidiQ8CI94.
KOiK00688.
OMAiMDQISSG.
OrthoDBiEOG7JQBMK.
PhylomeDBiQ8CI94.
TreeFamiTF300309.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CI94-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKPLTDSER QKQISVRGIA GLGDVAEVRK SFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYFFALAHTV RDHLVGRWIR TQQHYYERDP KRIYYLSLEF YMGRTLQNTM
110 120 130 140 150
VNLGLQTACD EATYQLGLDL EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEFGIFNQK IVNGWQVEEA DDWLRYGNPW EKARPEYMLP
210 220 230 240 250
VHFYGRVEHT PDGVLWLDTQ VVLAMPYDTP VPGYKNNTVN TMRLWSAKAP
260 270 280 290 300
NDFKLKDFNV GDYIEAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDIIR RFKSSRFGCR DPVRTCFETF PDKVAIQLND THPALSIPEL
360 370 380 390 400
MRILVDVEKV DWDKAWEITK KTCAYTNHTV LPEALERWPV SMFEKLLPRH
410 420 430 440 450
LEIIYAINQR HLDHVAALFP GDVDRLRRMS VIEEGDCKRI NMAHLCVIGS
460 470 480 490 500
HAVNGVARIH SEIVKQSVFK DFYELEPEKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
AEIIVERIGE GFLTDLSQLK KLLSLVDDEA FIRDVAKVKQ ENKLKFSAQL
560 570 580 590 600
EKEYKVKINP ASMFDVHVKR IHEYKRQLLN CLHIITLYNR IKKDPAKAFV
610 620 630 640 650
PRTVMIGGKA APGYHMAKMI IKLVTSIGDV VNHDPVVGDR LRVIFLENYR
660 670 680 690 700
VSLAEKVIPA ADLSQQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEENLF IFGMRVEDVE ALDQKGYNAR EFYERLPELR QAVDQISSGF
760 770 780 790 800
FSPKDPDCFK DVVNMLMYHD RFKVFADYEA YIQCQAQVDR LYRNSKEWTK
810 820 830 840
KVIRNIACSG KFSSDRTITE YAREIWGVEP SDLQIPPPNL PKD
Length:843
Mass (Da):96,730
Last modified:January 23, 2007 - v3
Checksum:iAF9F499FBB5B8B0C
GO

Sequence cautioni

The sequence AAH32209.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032209 mRNA. Translation: AAH32209.1. Different initiation.
BC035283 mRNA. Translation: AAH35283.1.
CCDSiCCDS16862.1.
RefSeqiNP_722476.1. NM_153781.1.
UniGeneiMm.222584.

Genome annotation databases

EnsembliENSMUST00000045441; ENSMUSP00000035743; ENSMUSG00000033059.
GeneIDi110078.
KEGGimmu:110078.
UCSCiuc008mul.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC032209 mRNA. Translation: AAH32209.1 . Different initiation.
BC035283 mRNA. Translation: AAH35283.1 .
CCDSi CCDS16862.1.
RefSeqi NP_722476.1. NM_153781.1.
UniGenei Mm.222584.

3D structure databases

ProteinModelPortali Q8CI94.
SMRi Q8CI94. Positions 14-839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 225278. 1 interaction.
IntActi Q8CI94. 4 interactions.
MINTi MINT-1870113.

Protein family/group databases

CAZyi GT35. Glycosyltransferase Family 35.

PTM databases

PhosphoSitei Q8CI94.

Proteomic databases

MaxQBi Q8CI94.
PaxDbi Q8CI94.
PRIDEi Q8CI94.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045441 ; ENSMUSP00000035743 ; ENSMUSG00000033059 .
GeneIDi 110078.
KEGGi mmu:110078.
UCSCi uc008mul.1. mouse.

Organism-specific databases

CTDi 5834.
MGIi MGI:97828. Pygb.

Phylogenomic databases

eggNOGi COG0058.
GeneTreei ENSGT00390000016886.
HOGENOMi HOG000278444.
HOVERGENi HBG006848.
InParanoidi Q8CI94.
KOi K00688.
OMAi MDQISSG.
OrthoDBi EOG7JQBMK.
PhylomeDBi Q8CI94.
TreeFami TF300309.

Enzyme and pathway databases

Reactomei REACT_256123. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi 363279.
PROi Q8CI94.
SOURCEi Search...

Gene expression databases

Bgeei Q8CI94.
CleanExi MM_PYGB.
ExpressionAtlasi Q8CI94. baseline and differential.
Genevestigatori Q8CI94.

Family and domain databases

InterProi IPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view ]
PANTHERi PTHR11468. PTHR11468. 1 hit.
Pfami PF00343. Phosphorylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsi TIGR02093. P_ylase. 1 hit.
PROSITEi PS00102. PHOSPHORYLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
  2. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-197 AND TYR-473, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiPYGB_MOUSE
AccessioniPrimary (citable) accession number: Q8CI94
Secondary accession number(s): Q8K283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3