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Q8CI94

- PYGB_MOUSE

UniProt

Q8CI94 - PYGB_MOUSE

Protein

Glycogen phosphorylase, brain form

Gene

Pygb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties By similarity.By similarity

    Catalytic activityi

    ((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Enzyme regulationi

    Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei76 – 761AMPBy similarity
    Sitei109 – 1091Involved in the association of subunitsBy similarity
    Sitei143 – 1431Involved in the association of subunitsBy similarity
    Sitei156 – 1561May be involved in allosteric controlBy similarity

    GO - Molecular functioni

    1. glycogen phosphorylase activity Source: InterPro
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. glycogen metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Protein family/group databases

    CAZyiGT35. Glycosyltransferase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen phosphorylase, brain form (EC:2.4.1.1)
    Gene namesi
    Name:Pygb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:97828. Pygb.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 843842Glycogen phosphorylase, brain formPRO_0000188536Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei15 – 151Phosphoserine; by PHK; in form phosphorylase ABy similarity
    Modified residuei197 – 1971Phosphotyrosine1 Publication
    Modified residuei473 – 4731Phosphotyrosine1 Publication
    Modified residuei681 – 6811N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8CI94.
    PaxDbiQ8CI94.
    PRIDEiQ8CI94.

    PTM databases

    PhosphoSiteiQ8CI94.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CI94.
    BgeeiQ8CI94.
    CleanExiMM_PYGB.
    GenevestigatoriQ8CI94.

    Interactioni

    Subunit structurei

    Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A By similarity.By similarity

    Protein-protein interaction databases

    BioGridi225278. 1 interaction.
    IntActiQ8CI94. 4 interactions.
    MINTiMINT-1870113.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CI94.
    SMRiQ8CI94. Positions 14-839.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycogen phosphorylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0058.
    GeneTreeiENSGT00390000016886.
    HOGENOMiHOG000278444.
    HOVERGENiHBG006848.
    InParanoidiQ8CI94.
    KOiK00688.
    OMAiMDQISSG.
    OrthoDBiEOG7JQBMK.
    PhylomeDBiQ8CI94.
    TreeFamiTF300309.

    Family and domain databases

    InterProiIPR011833. Glycg_phsphrylas.
    IPR000811. Glyco_trans_35.
    [Graphical view]
    PANTHERiPTHR11468. PTHR11468. 1 hit.
    PfamiPF00343. Phosphorylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
    TIGRFAMsiTIGR02093. P_ylase. 1 hit.
    PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CI94-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKPLTDSER QKQISVRGIA GLGDVAEVRK SFNRHLHFTL VKDRNVATPR    50
    DYFFALAHTV RDHLVGRWIR TQQHYYERDP KRIYYLSLEF YMGRTLQNTM 100
    VNLGLQTACD EATYQLGLDL EELEEIEEDA GLGNGGLGRL AACFLDSMAT 150
    LGLAAYGYGI RYEFGIFNQK IVNGWQVEEA DDWLRYGNPW EKARPEYMLP 200
    VHFYGRVEHT PDGVLWLDTQ VVLAMPYDTP VPGYKNNTVN TMRLWSAKAP 250
    NDFKLKDFNV GDYIEAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV 300
    VAATLQDIIR RFKSSRFGCR DPVRTCFETF PDKVAIQLND THPALSIPEL 350
    MRILVDVEKV DWDKAWEITK KTCAYTNHTV LPEALERWPV SMFEKLLPRH 400
    LEIIYAINQR HLDHVAALFP GDVDRLRRMS VIEEGDCKRI NMAHLCVIGS 450
    HAVNGVARIH SEIVKQSVFK DFYELEPEKF QNKTNGITPR RWLLLCNPGL 500
    AEIIVERIGE GFLTDLSQLK KLLSLVDDEA FIRDVAKVKQ ENKLKFSAQL 550
    EKEYKVKINP ASMFDVHVKR IHEYKRQLLN CLHIITLYNR IKKDPAKAFV 600
    PRTVMIGGKA APGYHMAKMI IKLVTSIGDV VNHDPVVGDR LRVIFLENYR 650
    VSLAEKVIPA ADLSQQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM 700
    AEEAGEENLF IFGMRVEDVE ALDQKGYNAR EFYERLPELR QAVDQISSGF 750
    FSPKDPDCFK DVVNMLMYHD RFKVFADYEA YIQCQAQVDR LYRNSKEWTK 800
    KVIRNIACSG KFSSDRTITE YAREIWGVEP SDLQIPPPNL PKD 843
    Length:843
    Mass (Da):96,730
    Last modified:January 23, 2007 - v3
    Checksum:iAF9F499FBB5B8B0C
    GO

    Sequence cautioni

    The sequence AAH32209.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC032209 mRNA. Translation: AAH32209.1. Different initiation.
    BC035283 mRNA. Translation: AAH35283.1.
    CCDSiCCDS16862.1.
    RefSeqiNP_722476.1. NM_153781.1.
    UniGeneiMm.222584.

    Genome annotation databases

    EnsembliENSMUST00000045441; ENSMUSP00000035743; ENSMUSG00000033059.
    GeneIDi110078.
    KEGGimmu:110078.
    UCSCiuc008mul.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC032209 mRNA. Translation: AAH32209.1 . Different initiation.
    BC035283 mRNA. Translation: AAH35283.1 .
    CCDSi CCDS16862.1.
    RefSeqi NP_722476.1. NM_153781.1.
    UniGenei Mm.222584.

    3D structure databases

    ProteinModelPortali Q8CI94.
    SMRi Q8CI94. Positions 14-839.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 225278. 1 interaction.
    IntActi Q8CI94. 4 interactions.
    MINTi MINT-1870113.

    Protein family/group databases

    CAZyi GT35. Glycosyltransferase Family 35.

    PTM databases

    PhosphoSitei Q8CI94.

    Proteomic databases

    MaxQBi Q8CI94.
    PaxDbi Q8CI94.
    PRIDEi Q8CI94.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045441 ; ENSMUSP00000035743 ; ENSMUSG00000033059 .
    GeneIDi 110078.
    KEGGi mmu:110078.
    UCSCi uc008mul.1. mouse.

    Organism-specific databases

    CTDi 5834.
    MGIi MGI:97828. Pygb.

    Phylogenomic databases

    eggNOGi COG0058.
    GeneTreei ENSGT00390000016886.
    HOGENOMi HOG000278444.
    HOVERGENi HBG006848.
    InParanoidi Q8CI94.
    KOi K00688.
    OMAi MDQISSG.
    OrthoDBi EOG7JQBMK.
    PhylomeDBi Q8CI94.
    TreeFami TF300309.

    Miscellaneous databases

    NextBioi 363279.
    PROi Q8CI94.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CI94.
    Bgeei Q8CI94.
    CleanExi MM_PYGB.
    Genevestigatori Q8CI94.

    Family and domain databases

    InterProi IPR011833. Glycg_phsphrylas.
    IPR000811. Glyco_trans_35.
    [Graphical view ]
    PANTHERi PTHR11468. PTHR11468. 1 hit.
    Pfami PF00343. Phosphorylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000460. Pprylas_GlgP. 1 hit.
    TIGRFAMsi TIGR02093. P_ylase. 1 hit.
    PROSITEi PS00102. PHOSPHORYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
    2. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-197 AND TYR-473, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiPYGB_MOUSE
    AccessioniPrimary (citable) accession number: Q8CI94
    Secondary accession number(s): Q8K283
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3