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Protein

Guanine nucleotide-binding protein-like 3

Gene

Gnl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be required to maintain the proliferative capacity of stem cells (By similarity). Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi176 – 179GTPSequence analysis4
Nucleotide bindingi256 – 263GTPSequence analysis8
Nucleotide bindingi300 – 303GTPSequence analysis4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: MGI
  • mRNA 5'-UTR binding Source: MGI

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein-like 3
Alternative name(s):
Nucleolar GTP-binding protein 3
Nucleostemin
Gene namesi
Name:Gnl3
Synonyms:Ns
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1353651 Gnl3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi256G → V: Mislocalized to the nucleoplasm. No effect on MDM2-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224451 – 538Guanine nucleotide-binding protein-like 3Add BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysineBy similarity1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei95PhosphoserineCombined sources1
Modified residuei101PhosphoserineBy similarity1
Cross-linki177Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki248Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki262Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki270Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei493PhosphoserineCombined sources1
Modified residuei505PhosphoserineCombined sources1
Modified residuei518PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CI11
PaxDbiQ8CI11
PeptideAtlasiQ8CI11
PRIDEiQ8CI11

PTM databases

iPTMnetiQ8CI11
PhosphoSitePlusiQ8CI11
SwissPalmiQ8CI11

Expressioni

Tissue specificityi

Expressed in the adult bone marrow population that is enriched in hematopoietic stem cells.1 Publication

Developmental stagei

Expressed at E8.5 and E10.5 in the cerebral cortex; expression declines rapidly from this point.1 Publication

Gene expression databases

BgeeiENSMUSG00000042354
GenevisibleiQ8CI11 MM

Interactioni

Subunit structurei

Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly. Indirectly interacts with TP53, via MDM2-binding (By similarity).By similarity

Protein-protein interaction databases

BioGridi205989, 2 interactors
IntActiQ8CI11, 1 interactor
MINTiQ8CI11
STRINGi10090.ENSMUSP00000047119

Structurei

3D structure databases

ProteinModelPortaliQ8CI11
SMRiQ8CI11
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 307CP-type GPROSITE-ProRule annotationAdd BLAST179

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 46BasicBy similarityAdd BLAST45
Regioni277 – 451IntermediateBy similarityAdd BLAST175
Regioni460 – 532AcidicBy similarityAdd BLAST73

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili54 – 95Sequence analysisAdd BLAST42

Domaini

The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis (By similarity).By similarity
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2484 Eukaryota
COG1161 LUCA
GeneTreeiENSGT00810000125472
HOGENOMiHOG000207716
HOVERGENiHBG051747
InParanoidiQ8CI11
KOiK14538
OMAiLLWSEWT
OrthoDBiEOG091G08IZ
PhylomeDBiQ8CI11
TreeFamiTF313085

Family and domain databases

Gene3Di1.10.1580.10, 1 hit
InterProiView protein in InterPro
IPR030378 G_CP_dom
IPR014813 Gnl3_N_dom
IPR023179 GTP-bd_ortho_bundle_sf
IPR006073 GTP_binding_domain
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF08701 GN3L_Grn1, 1 hit
PF01926 MMR_HSR1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51721 G_CP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CI11-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV
60 70 80 90 100
PNSAPFKEAL LREAELRKQQ LEELKQQQKL DRQKEQERKR KLEVSPGDEQ
110 120 130 140 150
SNVETREESD EPKRKKAKAG KQNPKKLHCQ ELKKVIEASD IVLEVLDARD
160 170 180 190 200
PLGCRCPQIE EAVIQSGSKK LILVLNKSDL VPKENLENWL NYLNKELPTV
210 220 230 240 250
VFKASTNLKN RKTFKIKKKK VVPFQSKICC GKEALWKLLG DFQQSCGKDI
260 270 280 290 300
QVGVIGFPNV GKSSVINSLK QEWICNVGIS MGLTRSMQIV PLDKQITIID
310 320 330 340 350
SPCLIISPCN SPTALALRSP ASIEELRPLE AASAILSQAD NEQVVLKYTV
360 370 380 390 400
PEYKDSLHFF TKLAQRRGLH QKGGSPNVES AAKLVWSEWT GASLGYYCHP
410 420 430 440 450
PASWNHSLHF NENIAAVMKK GFNLEELEKN NAHSIQVLKG PHLTNRILFR
460 470 480 490 500
SSGLTNGILD EKDIVEESPR QTEDKQDADD QENGSGERNA EISDVAPVEE
510 520 530
TRELSPEQST AGKPSDGSSA LDRASQEDET YDFTTDYI
Length:538
Mass (Da):60,786
Last modified:March 1, 2004 - v2
Checksum:iC092AD5305E5D69F
GO
Isoform 2 (identifier: Q8CI11-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     465-497: Missing.

Note: No experimental confirmation available.
Show »
Length:505
Mass (Da):57,188
Checksum:i99B975B106ABC9B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti470R → S in AAO19472 (PubMed:12464630).Curated1
Sequence conflicti475K → Q in AAO19472 (PubMed:12464630).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013412465 – 497Missing in isoform 2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181025 mRNA Translation: AAO19472.1
AY185498 mRNA Translation: AAO19473.1
AK077523 mRNA Translation: BAC36844.1
BC037996 mRNA Translation: AAH37996.2
CCDSiCCDS26905.1 [Q8CI11-1]
RefSeqiNP_705775.2, NM_153547.6 [Q8CI11-1]
UniGeneiMm.88512

Genome annotation databases

EnsembliENSMUST00000037739; ENSMUSP00000047119; ENSMUSG00000042354 [Q8CI11-1]
GeneIDi30877
KEGGimmu:30877
UCSCiuc007swi.3 mouse [Q8CI11-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGNL3_MOUSE
AccessioniPrimary (citable) accession number: Q8CI11
Secondary accession number(s): Q811R9, Q811S8, Q8BK21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2004
Last modified: June 20, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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