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Protein

Guanine nucleotide-binding protein-like 3

Gene

Gnl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be required to maintain the proliferative capacity of stem cells (By similarity). Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi176 – 1794GTPSequence analysis
Nucleotide bindingi256 – 2638GTPSequence analysis
Nucleotide bindingi300 – 3034GTPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein-like 3
Alternative name(s):
Nucleolar GTP-binding protein 3
Nucleostemin
Gene namesi
Name:Gnl3
Synonyms:Ns
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1353651. Gnl3.

Subcellular locationi

  • Nucleus 1 Publication
  • Nucleusnucleolus 1 Publication

  • Note: Shuttles between the nucleus and nucleolus.By similarity

GO - Cellular componenti

  • extracellular space Source: MGI
  • membrane Source: MGI
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi256 – 2561G → V: Mislocalized to the nucleoplasm. No effect on MDM2-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Guanine nucleotide-binding protein-like 3PRO_0000122445Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei79 – 791N6-acetyllysineBy similarity
Modified residuei95 – 951PhosphoserineCombined sources
Modified residuei101 – 1011PhosphoserineBy similarity
Modified residuei493 – 4931PhosphoserineCombined sources
Modified residuei505 – 5051PhosphoserineCombined sources
Modified residuei518 – 5181PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CI11.
MaxQBiQ8CI11.
PaxDbiQ8CI11.
PRIDEiQ8CI11.

PTM databases

iPTMnetiQ8CI11.
PhosphoSiteiQ8CI11.

Expressioni

Tissue specificityi

Expressed in the adult bone marrow population that is enriched in hematopoietic stem cells.1 Publication

Developmental stagei

Expressed at E8.5 and E10.5 in the cerebral cortex; expression declines rapidly from this point.1 Publication

Gene expression databases

BgeeiQ8CI11.
GenevisibleiQ8CI11. MM.

Interactioni

Subunit structurei

Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly. Indirectly interacts with TP53, via MDM2-binding (By similarity).By similarity

Protein-protein interaction databases

BioGridi205989. 3 interactions.
IntActiQ8CI11. 1 interaction.
MINTiMINT-1867955.
STRINGi10090.ENSMUSP00000047119.

Structurei

3D structure databases

ProteinModelPortaliQ8CI11.
SMRiQ8CI11. Positions 130-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 307179CP-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 4645BasicBy similarityAdd
BLAST
Regioni277 – 451175IntermediateBy similarityAdd
BLAST
Regioni460 – 53273AcidicBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili54 – 9542Sequence analysisAdd
BLAST

Domaini

The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis (By similarity).By similarity
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family.PROSITE-ProRule annotation
Contains 1 CP-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2484. Eukaryota.
COG1161. LUCA.
GeneTreeiENSGT00810000125472.
HOGENOMiHOG000207716.
HOVERGENiHBG051747.
InParanoidiQ8CI11.
KOiK14538.
OMAiEVCFGKE.
OrthoDBiEOG7J9VPR.
PhylomeDBiQ8CI11.
TreeFamiTF313085.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR014813. Gnl3_N_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08701. GN3L_Grn1. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CI11-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV
60 70 80 90 100
PNSAPFKEAL LREAELRKQQ LEELKQQQKL DRQKEQERKR KLEVSPGDEQ
110 120 130 140 150
SNVETREESD EPKRKKAKAG KQNPKKLHCQ ELKKVIEASD IVLEVLDARD
160 170 180 190 200
PLGCRCPQIE EAVIQSGSKK LILVLNKSDL VPKENLENWL NYLNKELPTV
210 220 230 240 250
VFKASTNLKN RKTFKIKKKK VVPFQSKICC GKEALWKLLG DFQQSCGKDI
260 270 280 290 300
QVGVIGFPNV GKSSVINSLK QEWICNVGIS MGLTRSMQIV PLDKQITIID
310 320 330 340 350
SPCLIISPCN SPTALALRSP ASIEELRPLE AASAILSQAD NEQVVLKYTV
360 370 380 390 400
PEYKDSLHFF TKLAQRRGLH QKGGSPNVES AAKLVWSEWT GASLGYYCHP
410 420 430 440 450
PASWNHSLHF NENIAAVMKK GFNLEELEKN NAHSIQVLKG PHLTNRILFR
460 470 480 490 500
SSGLTNGILD EKDIVEESPR QTEDKQDADD QENGSGERNA EISDVAPVEE
510 520 530
TRELSPEQST AGKPSDGSSA LDRASQEDET YDFTTDYI
Length:538
Mass (Da):60,786
Last modified:March 1, 2004 - v2
Checksum:iC092AD5305E5D69F
GO
Isoform 2 (identifier: Q8CI11-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     465-497: Missing.

Note: No experimental confirmation available.
Show »
Length:505
Mass (Da):57,188
Checksum:i99B975B106ABC9B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti470 – 4701R → S in AAO19472 (PubMed:12464630).Curated
Sequence conflicti475 – 4751K → Q in AAO19472 (PubMed:12464630).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei465 – 49733Missing in isoform 2. 1 PublicationVSP_013412Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181025 mRNA. Translation: AAO19472.1.
AY185498 mRNA. Translation: AAO19473.1.
AK077523 mRNA. Translation: BAC36844.1.
BC037996 mRNA. Translation: AAH37996.2.
CCDSiCCDS26905.1. [Q8CI11-1]
RefSeqiNP_705775.2. NM_153547.6. [Q8CI11-1]
UniGeneiMm.88512.

Genome annotation databases

EnsembliENSMUST00000037739; ENSMUSP00000047119; ENSMUSG00000042354. [Q8CI11-1]
GeneIDi30877.
KEGGimmu:30877.
UCSCiuc007swi.3. mouse. [Q8CI11-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY181025 mRNA. Translation: AAO19472.1.
AY185498 mRNA. Translation: AAO19473.1.
AK077523 mRNA. Translation: BAC36844.1.
BC037996 mRNA. Translation: AAH37996.2.
CCDSiCCDS26905.1. [Q8CI11-1]
RefSeqiNP_705775.2. NM_153547.6. [Q8CI11-1]
UniGeneiMm.88512.

3D structure databases

ProteinModelPortaliQ8CI11.
SMRiQ8CI11. Positions 130-388.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205989. 3 interactions.
IntActiQ8CI11. 1 interaction.
MINTiMINT-1867955.
STRINGi10090.ENSMUSP00000047119.

PTM databases

iPTMnetiQ8CI11.
PhosphoSiteiQ8CI11.

Proteomic databases

EPDiQ8CI11.
MaxQBiQ8CI11.
PaxDbiQ8CI11.
PRIDEiQ8CI11.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037739; ENSMUSP00000047119; ENSMUSG00000042354. [Q8CI11-1]
GeneIDi30877.
KEGGimmu:30877.
UCSCiuc007swi.3. mouse. [Q8CI11-1]

Organism-specific databases

CTDi26354.
MGIiMGI:1353651. Gnl3.

Phylogenomic databases

eggNOGiKOG2484. Eukaryota.
COG1161. LUCA.
GeneTreeiENSGT00810000125472.
HOGENOMiHOG000207716.
HOVERGENiHBG051747.
InParanoidiQ8CI11.
KOiK14538.
OMAiEVCFGKE.
OrthoDBiEOG7J9VPR.
PhylomeDBiQ8CI11.
TreeFamiTF313085.

Miscellaneous databases

ChiTaRSiGnl3. mouse.
PROiQ8CI11.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CI11.
GenevisibleiQ8CI11. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR030378. G_CP_dom.
IPR014813. Gnl3_N_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF08701. GN3L_Grn1. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A nucleolar mechanism controlling cell proliferation in stem and cancer cells."
    Tsai R.Y.L., McKay R.D.G.
    Genes Dev. 16:2991-3003(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95; SER-493 AND SER-505, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung, Pancreas and Spleen.
  6. "GNL3L depletion destabilizes MDM2 and induces p53-dependent G2/M arrest."
    Meng L., Hsu J.K., Tsai R.Y.
    Oncogene 30:1716-1726(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MDM2, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-256.

Entry informationi

Entry nameiGNL3_MOUSE
AccessioniPrimary (citable) accession number: Q8CI11
Secondary accession number(s): Q811R9, Q811S8, Q8BK21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2004
Last modified: June 8, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.