Q8CHX6 (MBTP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 71.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Membrane-bound transcription factor site-2 protease EC=3.4.24.85 Alternative name(s): Endopeptidase S2P | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 515 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Intramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop By similarity. |
| Catalytic activity | Cleaves several transcription factors that are type-2 transmembrane proteins within membrane-spanning domains. Known substrates include sterol regulatory element-binding protein (SREBP) -1, SREBP-2 and forms of the transcriptional activator ATF6. SREBP-2 is cleaved at the site 477-DRSRILL-|-CVLTFLCLSFNPLTSLLQWGGA-505. The residues Asn-Pro, 11 residues distal to the site of cleavage in the membrane-spanning domain, are important for cleavage by S2P endopeptidase. Replacement of either of these residues does not prevent cleavage, but there is no cleavage if both of these residues are replaced. |
| Cofactor | Binds 1 zinc ion per subunit By similarity. |
| Subcellular location | Membrane; Multi-pass membrane protein Probable. Cytoplasm By similarity. |
| Sequence similarities | Belongs to the peptidase M50A family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cholesterol metabolism Lipid metabolism Steroid metabolism |
| Cellular component | Cytoplasm Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| PTM | Glycoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 515 | 515 | Membrane-bound transcription factor site-2 protease | PRO_0000261592 | |||||
Regions | |||||||||
| Topological domain | 1 – 3 | 3 | Cytoplasmic By similarity | ||||||
| Transmembrane | 4 – 24 | 21 | Helical; Potential | ||||||
| Topological domain | 25 – 74 | 50 | Lumenal By similarity | ||||||
| Transmembrane | 75 – 95 | 21 | Helical; Potential | ||||||
| Transmembrane | 96 – 107 | 12 | Helical; Potential | ||||||
| Topological domain | 108 – 140 | 33 | Lumenal By similarity | ||||||
| Transmembrane | 141 – 165 | 25 | Helical; Potential | ||||||
| Transmembrane | 170 – 182 | 13 | Helical; Potential | ||||||
| Transmembrane | 183 – 205 | 23 | Helical; Potential | ||||||
| Transmembrane | 225 – 247 | 23 | Helical; Potential | ||||||
| Topological domain | 248 – 442 | 195 | Lumenal By similarity | ||||||
| Transmembrane | 443 – 460 | 18 | Helical; Potential | ||||||
| Transmembrane | 461 – 472 | 12 | Helical; Potential | ||||||
| Topological domain | 473 – 488 | 16 | Lumenal Potential | ||||||
| Transmembrane | 489 – 509 | 21 | Helical; Potential | ||||||
| Topological domain | 510 – 515 | 6 | Cytoplasmic Potential | ||||||
| Compositional bias | 114 – 132 | 19 | Poly-Ser | ||||||
| Compositional bias | 281 – 382 | 102 | Cys-rich | ||||||
Sites | |||||||||
| Active site | 168 | 1 | By similarity | ||||||
| Metal binding | 167 | 1 | Zinc; catalytic By similarity | ||||||
| Metal binding | 171 | 1 | Zinc; catalytic By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 333 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK148325 mRNA. Translation: BAE28482.1. AK164486 mRNA. Translation: BAE37807.1. AL663072 Genomic DNA. Translation: CAM18093.1. BC038343 mRNA. Translation: AAH38343.1. |
| IPI | IPI00229772. |
| RefSeq | NP_758511.1. NM_172307.3. |
| UniGene | Mm.37577. |
3D structure databases | |
| ProteinModelPortal | Q8CHX6. |
| SMR | Q8CHX6. Positions 157-234, 249-294. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q8CHX6. |
Protein family/group databases | |
| MEROPS | M50.001. |
Proteomic databases | |
| PRIDE | Q8CHX6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000058098; ENSMUSP00000059471; ENSMUSG00000046873. |
| GeneID | 270669. |
| KEGG | mmu:270669. |
| NMPDR | fig|10090.3.peg.22376. |
| UCSC | uc009usb.1. mouse. |
Organism-specific databases | |
| CTD | 51360. |
| MGI | MGI:2444506. Mbtps2. |
Phylogenomic databases | |
| GeneTree | ENSGT00510000048066. |
| HOVERGEN | HBG006397. |
| OMA | SLENQTR. |
Gene expression databases | |
| ArrayExpress | Q8CHX6. |
| Bgee | Q8CHX6. |
| Genevestigator | Q8CHX6. |
| GermOnline | ENSMUSG00000046873. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001193. Pept_M50_SREBP. IPR008915. Peptidase_M50. [Graphical view] |
| KO | K07765. |
| Pfam | PF02163. Peptidase_M50. 1 hit. [Graphical view] |
| PRINTS | PR01000. SREBPS2PTASE. |
| PROSITE | PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 393364. |
| SOURCE | Search... |
Entry information
| Entry name | MBTP2_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8CHX6 Secondary accession number(s): A2AC85 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with