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Q8CHU3

- EPN2_MOUSE

UniProt

Q8CHU3 - EPN2_MOUSE

Protein

Epsin-2

Gene

Epn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Plays a role in the formation of clathrin-coated invaginations and endocytosis.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81Phosphatidylinositol lipid headgroupBy similarity
    Binding sitei11 – 111Phosphatidylinositol lipid headgroupBy similarity
    Binding sitei25 – 251Phosphatidylinositol lipid headgroupBy similarity
    Binding sitei30 – 301Phosphatidylinositol lipid headgroupBy similarity
    Binding sitei63 – 631Phosphatidylinositol lipid headgroupBy similarity
    Binding sitei73 – 731Phosphatidylinositol lipid headgroupBy similarity

    GO - Molecular functioni

    1. lipid binding Source: UniProtKB-KW

    GO - Biological processi

    1. embryonic organ development Source: MGI
    2. endocytosis Source: UniProtKB-KW
    3. in utero embryonic development Source: MGI
    4. Notch signaling pathway Source: MGI

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epsin-2
    Alternative name(s):
    EPS-15-interacting protein 2
    Intersectin-EH-binding protein 2
    Short name:
    Ibp2
    Gene namesi
    Name:Epn2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1333766. Epn2.

    Subcellular locationi

    Cytoplasm By similarity
    Note: In punctate structures throughout the cell and particularly concentrated in the region of the Golgi complex.By similarity

    GO - Cellular componenti

    1. clathrin coat of endocytic vesicle Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 595595Epsin-2PRO_0000074517Add
    BLAST

    Post-translational modificationi

    Ubiquitinated.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiQ8CHU3.
    PaxDbiQ8CHU3.
    PRIDEiQ8CHU3.

    PTM databases

    PhosphoSiteiQ8CHU3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CHU3.
    BgeeiQ8CHU3.
    CleanExiMM_EPN2.
    GenevestigatoriQ8CHU3.

    Interactioni

    Subunit structurei

    Binds EPS15, AP-2 and clathrin.By similarity

    Protein-protein interaction databases

    BioGridi199486. 3 interactions.
    IntActiQ8CHU3. 3 interactions.
    MINTiMINT-93098.
    STRINGi10090.ENSMUSP00000104352.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CHU3.
    SMRiQ8CHU3. Positions 1-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 144133ENTHPROSITE-ProRule annotationAdd
    BLAST
    Repeati218 – 23720UIM 1Add
    BLAST
    Repeati255 – 27420UIM 2Add
    BLAST
    Repeati313 – 31531
    Repeati325 – 32732
    Repeati338 – 34033
    Repeati352 – 35434
    Repeati370 – 37235
    Repeati387 – 38936
    Repeati494 – 49631
    Repeati508 – 51032
    Repeati591 – 59333

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni313 – 389776 X 3 AA repeats of [DE]-P-WAdd
    BLAST
    Regioni494 – 5931003 X 3 AA repeats of N-P-FAdd
    BLAST

    Domaini

    The NPF repeat domain is involved in EPS15 binding.
    The DPW repeat domain is involved in AP-2 and clathrin binding.

    Sequence similaritiesi

    Belongs to the epsin family.Curated
    Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation
    Contains 2 UIM (ubiquitin-interacting motif) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG263730.
    GeneTreeiENSGT00550000074611.
    HOGENOMiHOG000008298.
    HOVERGENiHBG006690.
    KOiK12471.
    PhylomeDBiQ8CHU3.
    TreeFamiTF313361.

    Family and domain databases

    Gene3Di1.25.40.90. 1 hit.
    InterProiIPR008942. ENTH_VHS.
    IPR027319. Epsin-2_metazoa.
    IPR013809. Epsin-like_N.
    IPR001026. Epsin_dom_N.
    IPR003903. Ubiquitin-int_motif.
    [Graphical view]
    PANTHERiPTHR12276:SF50. PTHR12276:SF50. 1 hit.
    PfamiPF01417. ENTH. 1 hit.
    [Graphical view]
    SMARTiSM00273. ENTH. 1 hit.
    SM00726. UIM. 2 hits.
    [Graphical view]
    SUPFAMiSSF48464. SSF48464. 1 hit.
    PROSITEiPS50942. ENTH. 1 hit.
    PS50330. UIM. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8CHU3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV    50
    VAFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI 100
    FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KVERVQALKT 150
    KERMAQVATG VGSNQITFGR GSSQPNLSTS YSEQEYGKAG GSPASYHGST 200
    SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQSSES VQTARGSKEE 250
    RLRRGDDLRL QMALEESRRD TVKVPKKKEA KACCKPGSHS QQTTLLDLMD 300
    ALPSSGPVTQ KTEPWSAGAS ANQTNPWGGT VAPSNITDPW PSFGTKPAAS 350
    VDPWGVPTTA STQSVPKNSD PWAASQQPAS NAGKTTDAWG AAKPSSASGS 400
    FELFSNFNGT VKDDFSEFDN LRTSKKPAES GASVPPQDSR TTSPDLFESQ 450
    SLTSASSKPS SARKTPESFL GPNAALVNLD SLVTKPAPPA QSLNPFLAPG 500
    AAAPAPVNPF QVNQPQPLTL NQLRGSPVLG SSASFGSGPG VETVAPMTSV 550
    APHSSVGASG SSLTPLGPTA MNMVGSVGIP PSAAQSTGTT NPFLL 595
    Length:595
    Mass (Da):63,472
    Last modified:March 1, 2003 - v1
    Checksum:i255DC7BC7E1B2D13
    GO
    Isoform 2 (identifier: Q8CHU3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         237-248: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:583
    Mass (Da):62,253
    Checksum:iEFC06A1371A85980
    GO

    Sequence cautioni

    The sequence AAC97476.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti75 – 751Y → S in AAC97476. (PubMed:9813051)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei237 – 24812Missing in isoform 2. 2 PublicationsVSP_009157Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK036331 mRNA. Translation: BAC29387.1.
    AL604029 Genomic DNA. Translation: CAI24192.1.
    BC039138 mRNA. Translation: AAH39138.1.
    AF057286 mRNA. Translation: AAC97476.1. Different initiation.
    CCDSiCCDS24816.1. [Q8CHU3-1]
    CCDS56779.1. [Q8CHU3-2]
    RefSeqiNP_001239118.1. NM_001252189.1. [Q8CHU3-2]
    NP_034278.1. NM_010148.3. [Q8CHU3-1]
    UniGeneiMm.139695.
    Mm.489800.

    Genome annotation databases

    EnsembliENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036. [Q8CHU3-1]
    ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036. [Q8CHU3-2]
    ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036. [Q8CHU3-1]
    GeneIDi13855.
    KEGGimmu:13855.
    UCSCiuc007jhv.2. mouse. [Q8CHU3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK036331 mRNA. Translation: BAC29387.1 .
    AL604029 Genomic DNA. Translation: CAI24192.1 .
    BC039138 mRNA. Translation: AAH39138.1 .
    AF057286 mRNA. Translation: AAC97476.1 . Different initiation.
    CCDSi CCDS24816.1. [Q8CHU3-1 ]
    CCDS56779.1. [Q8CHU3-2 ]
    RefSeqi NP_001239118.1. NM_001252189.1. [Q8CHU3-2 ]
    NP_034278.1. NM_010148.3. [Q8CHU3-1 ]
    UniGenei Mm.139695.
    Mm.489800.

    3D structure databases

    ProteinModelPortali Q8CHU3.
    SMRi Q8CHU3. Positions 1-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199486. 3 interactions.
    IntActi Q8CHU3. 3 interactions.
    MINTi MINT-93098.
    STRINGi 10090.ENSMUSP00000104352.

    PTM databases

    PhosphoSitei Q8CHU3.

    Proteomic databases

    MaxQBi Q8CHU3.
    PaxDbi Q8CHU3.
    PRIDEi Q8CHU3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000001063 ; ENSMUSP00000001063 ; ENSMUSG00000001036 . [Q8CHU3-1 ]
    ENSMUST00000108713 ; ENSMUSP00000104353 ; ENSMUSG00000001036 . [Q8CHU3-2 ]
    ENSMUST00000178202 ; ENSMUSP00000136553 ; ENSMUSG00000001036 . [Q8CHU3-1 ]
    GeneIDi 13855.
    KEGGi mmu:13855.
    UCSCi uc007jhv.2. mouse. [Q8CHU3-1 ]

    Organism-specific databases

    CTDi 22905.
    MGIi MGI:1333766. Epn2.

    Phylogenomic databases

    eggNOGi NOG263730.
    GeneTreei ENSGT00550000074611.
    HOGENOMi HOG000008298.
    HOVERGENi HBG006690.
    KOi K12471.
    PhylomeDBi Q8CHU3.
    TreeFami TF313361.

    Miscellaneous databases

    ChiTaRSi EPN2. mouse.
    NextBioi 284722.
    PROi Q8CHU3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CHU3.
    Bgeei Q8CHU3.
    CleanExi MM_EPN2.
    Genevestigatori Q8CHU3.

    Family and domain databases

    Gene3Di 1.25.40.90. 1 hit.
    InterProi IPR008942. ENTH_VHS.
    IPR027319. Epsin-2_metazoa.
    IPR013809. Epsin-like_N.
    IPR001026. Epsin_dom_N.
    IPR003903. Ubiquitin-int_motif.
    [Graphical view ]
    PANTHERi PTHR12276:SF50. PTHR12276:SF50. 1 hit.
    Pfami PF01417. ENTH. 1 hit.
    [Graphical view ]
    SMARTi SM00273. ENTH. 1 hit.
    SM00726. UIM. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48464. SSF48464. 1 hit.
    PROSITEi PS50942. ENTH. 1 hit.
    PS50330. UIM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "Intersectin, a novel adaptor protein with two eps15 homology and five src homology 3 domains."
      Yamabhai M., Hoffman N.G., Hardison N.L., McPherson P.S., Castagnoli L., Cesareni G., Kay B.K.
      J. Biol. Chem. 273:31401-31407(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-595 (ISOFORM 2), INTERACTION WITH ITSN1.
    5. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 77-86, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.

    Entry informationi

    Entry nameiEPN2_MOUSE
    AccessioniPrimary (citable) accession number: Q8CHU3
    Secondary accession number(s): O70447, Q5NCM4, Q8BZ85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3