Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8CHU3 (EPN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epsin-2
Alternative name(s):
EPS-15-interacting protein 2
Intersectin-EH-binding protein 2
Short name=Ibp2
Gene names
Name:Epn2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length595 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in the formation of clathrin-coated invaginations and endocytosis By similarity.

Subunit structure

Binds EPS15, AP-2 and clathrin By similarity.

Subcellular location

Cytoplasm By similarity. Note: In punctate structures throughout the cell and particularly concentrated in the region of the Golgi complex By similarity.

Domain

The NPF repeat domain is involved in EPS15 binding.

The DPW repeat domain is involved in AP-2 and clathrin binding.

Post-translational modification

Ubiquitinated By similarity.

Sequence similarities

Belongs to the epsin family.

Contains 1 ENTH (epsin N-terminal homology) domain.

Contains 2 UIM (ubiquitin-interacting motif) repeats.

Sequence caution

The sequence AAC97476.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8CHU3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8CHU3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     237-248: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 595595Epsin-2
PRO_0000074517

Regions

Domain12 – 144133ENTH
Repeat218 – 23720UIM 1
Repeat255 – 27420UIM 2
Repeat313 – 31531
Repeat325 – 32732
Repeat338 – 34033
Repeat352 – 35434
Repeat370 – 37235
Repeat387 – 38936
Repeat494 – 49631
Repeat508 – 51032
Repeat591 – 59333
Region313 – 389776 X 3 AA repeats of [DE]-P-W
Region494 – 5931003 X 3 AA repeats of N-P-F

Sites

Binding site81Phosphatidylinositol lipid headgroup By similarity
Binding site111Phosphatidylinositol lipid headgroup By similarity
Binding site251Phosphatidylinositol lipid headgroup By similarity
Binding site301Phosphatidylinositol lipid headgroup By similarity
Binding site631Phosphatidylinositol lipid headgroup By similarity
Binding site731Phosphatidylinositol lipid headgroup By similarity

Natural variations

Alternative sequence237 – 24812Missing in isoform 2.
VSP_009157

Experimental info

Sequence conflict751Y → S in AAC97476. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 255DC7BC7E1B2D13

FASTA59563,472
        10         20         30         40         50         60 
MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV VAFSEIMSMV 

        70         80         90        100        110        120 
WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI FAIQTLKDFQ YIDRDGKDQG 

       130        140        150        160        170        180 
INVREKSKQL VALLKDEERL KVERVQALKT KERMAQVATG VGSNQITFGR GSSQPNLSTS 

       190        200        210        220        230        240 
YSEQEYGKAG GSPASYHGST SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQSSES 

       250        260        270        280        290        300 
VQTARGSKEE RLRRGDDLRL QMALEESRRD TVKVPKKKEA KACCKPGSHS QQTTLLDLMD 

       310        320        330        340        350        360 
ALPSSGPVTQ KTEPWSAGAS ANQTNPWGGT VAPSNITDPW PSFGTKPAAS VDPWGVPTTA 

       370        380        390        400        410        420 
STQSVPKNSD PWAASQQPAS NAGKTTDAWG AAKPSSASGS FELFSNFNGT VKDDFSEFDN 

       430        440        450        460        470        480 
LRTSKKPAES GASVPPQDSR TTSPDLFESQ SLTSASSKPS SARKTPESFL GPNAALVNLD 

       490        500        510        520        530        540 
SLVTKPAPPA QSLNPFLAPG AAAPAPVNPF QVNQPQPLTL NQLRGSPVLG SSASFGSGPG 

       550        560        570        580        590 
VETVAPMTSV APHSSVGASG SSLTPLGPTA MNMVGSVGIP PSAAQSTGTT NPFLL 

« Hide

Isoform 2 [UniParc].

Checksum: EFC06A1371A85980
Show »

FASTA58362,253

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Cerebellum.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Intersectin, a novel adaptor protein with two eps15 homology and five src homology 3 domains."
Yamabhai M., Hoffman N.G., Hardison N.L., McPherson P.S., Castagnoli L., Cesareni G., Kay B.K.
J. Biol. Chem. 273:31401-31407(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-595 (ISOFORM 2), INTERACTION WITH ITSN1.
[5]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 77-86, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[6]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK036331 mRNA. Translation: BAC29387.1.
AL604029 Genomic DNA. Translation: CAI24192.1.
BC039138 mRNA. Translation: AAH39138.1.
AF057286 mRNA. Translation: AAC97476.1. Different initiation.
RefSeqNP_001239118.1. NM_001252189.1.
NP_034278.1. NM_010148.3.
UniGeneMm.139695.
Mm.489800.

3D structure databases

ProteinModelPortalQ8CHU3.
SMRQ8CHU3. Positions 1-144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199486. 2 interactions.
IntActQ8CHU3. 3 interactions.
MINTMINT-93098.
STRING10090.ENSMUSP00000104352.

PTM databases

PhosphoSiteQ8CHU3.

Proteomic databases

PaxDbQ8CHU3.
PRIDEQ8CHU3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036. [Q8CHU3-1]
ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036. [Q8CHU3-2]
ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036. [Q8CHU3-1]
GeneID13855.
KEGGmmu:13855.
UCSCuc007jhv.2. mouse. [Q8CHU3-1]

Organism-specific databases

CTD22905.
MGIMGI:1333766. Epn2.

Phylogenomic databases

eggNOGNOG263730.
GeneTreeENSGT00550000074611.
HOGENOMHOG000008298.
HOVERGENHBG006690.
KOK12471.
PhylomeDBQ8CHU3.
TreeFamTF313361.

Gene expression databases

ArrayExpressQ8CHU3.
BgeeQ8CHU3.
CleanExMM_EPN2.
GenevestigatorQ8CHU3.

Family and domain databases

Gene3D1.25.40.90. 1 hit.
InterProIPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
IPR001026. Epsin_dom_N.
IPR003903. Ubiquitin-int_motif.
[Graphical view]
PfamPF01417. ENTH. 1 hit.
[Graphical view]
SMARTSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMSSF48464. SSF48464. 1 hit.
PROSITEPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEPN2. mouse.
NextBio284722.
PROQ8CHU3.
SOURCESearch...

Entry information

Entry nameEPN2_MOUSE
AccessionPrimary (citable) accession number: Q8CHU3
Secondary accession number(s): O70447, Q5NCM4, Q8BZ85
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot