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Q8CHU3

- EPN2_MOUSE

UniProt

Q8CHU3 - EPN2_MOUSE

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Protein
Epsin-2
Gene
Epn2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in the formation of clathrin-coated invaginations and endocytosis By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 81Phosphatidylinositol lipid headgroup By similarity
Binding sitei11 – 111Phosphatidylinositol lipid headgroup By similarity
Binding sitei25 – 251Phosphatidylinositol lipid headgroup By similarity
Binding sitei30 – 301Phosphatidylinositol lipid headgroup By similarity
Binding sitei63 – 631Phosphatidylinositol lipid headgroup By similarity
Binding sitei73 – 731Phosphatidylinositol lipid headgroup By similarity

GO - Molecular functioni

  1. lipid binding Source: UniProtKB-KW

GO - Biological processi

  1. Notch signaling pathway Source: MGI
  2. embryonic organ development Source: MGI
  3. endocytosis Source: UniProtKB-KW
  4. in utero embryonic development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-2
Alternative name(s):
EPS-15-interacting protein 2
Intersectin-EH-binding protein 2
Short name:
Ibp2
Gene namesi
Name:Epn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1333766. Epn2.

Subcellular locationi

Cytoplasm By similarity
Note: In punctate structures throughout the cell and particularly concentrated in the region of the Golgi complex By similarity.

GO - Cellular componenti

  1. clathrin coat of endocytic vesicle Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Epsin-2
PRO_0000074517Add
BLAST

Post-translational modificationi

Ubiquitinated By similarity.

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ8CHU3.
PaxDbiQ8CHU3.
PRIDEiQ8CHU3.

PTM databases

PhosphoSiteiQ8CHU3.

Expressioni

Gene expression databases

ArrayExpressiQ8CHU3.
BgeeiQ8CHU3.
CleanExiMM_EPN2.
GenevestigatoriQ8CHU3.

Interactioni

Subunit structurei

Binds EPS15, AP-2 and clathrin By similarity.

Protein-protein interaction databases

BioGridi199486. 3 interactions.
IntActiQ8CHU3. 3 interactions.
MINTiMINT-93098.
STRINGi10090.ENSMUSP00000104352.

Structurei

3D structure databases

ProteinModelPortaliQ8CHU3.
SMRiQ8CHU3. Positions 1-144.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 144133ENTH
Add
BLAST
Repeati218 – 23720UIM 1
Add
BLAST
Repeati255 – 27420UIM 2
Add
BLAST
Repeati313 – 31531
Repeati325 – 32732
Repeati338 – 34033
Repeati352 – 35434
Repeati370 – 37235
Repeati387 – 38936
Repeati494 – 49631
Repeati508 – 51032
Repeati591 – 59333

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni313 – 389776 X 3 AA repeats of [DE]-P-W
Add
BLAST
Regioni494 – 5931003 X 3 AA repeats of N-P-F
Add
BLAST

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.

Sequence similaritiesi

Belongs to the epsin family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG263730.
GeneTreeiENSGT00550000074611.
HOGENOMiHOG000008298.
HOVERGENiHBG006690.
KOiK12471.
PhylomeDBiQ8CHU3.
TreeFamiTF313361.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR027319. Epsin-2_metazoa.
IPR013809. Epsin-like_N.
IPR001026. Epsin_dom_N.
IPR003903. Ubiquitin-int_motif.
[Graphical view]
PANTHERiPTHR12276:SF50. PTHR12276:SF50. 1 hit.
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8CHU3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV    50
VAFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI 100
FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KVERVQALKT 150
KERMAQVATG VGSNQITFGR GSSQPNLSTS YSEQEYGKAG GSPASYHGST 200
SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQSSES VQTARGSKEE 250
RLRRGDDLRL QMALEESRRD TVKVPKKKEA KACCKPGSHS QQTTLLDLMD 300
ALPSSGPVTQ KTEPWSAGAS ANQTNPWGGT VAPSNITDPW PSFGTKPAAS 350
VDPWGVPTTA STQSVPKNSD PWAASQQPAS NAGKTTDAWG AAKPSSASGS 400
FELFSNFNGT VKDDFSEFDN LRTSKKPAES GASVPPQDSR TTSPDLFESQ 450
SLTSASSKPS SARKTPESFL GPNAALVNLD SLVTKPAPPA QSLNPFLAPG 500
AAAPAPVNPF QVNQPQPLTL NQLRGSPVLG SSASFGSGPG VETVAPMTSV 550
APHSSVGASG SSLTPLGPTA MNMVGSVGIP PSAAQSTGTT NPFLL 595
Length:595
Mass (Da):63,472
Last modified:March 1, 2003 - v1
Checksum:i255DC7BC7E1B2D13
GO
Isoform 2 (identifier: Q8CHU3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     237-248: Missing.

Note: No experimental confirmation available.

Show »
Length:583
Mass (Da):62,253
Checksum:iEFC06A1371A85980
GO

Sequence cautioni

The sequence AAC97476.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei237 – 24812Missing in isoform 2.
VSP_009157Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751Y → S in AAC97476. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK036331 mRNA. Translation: BAC29387.1.
AL604029 Genomic DNA. Translation: CAI24192.1.
BC039138 mRNA. Translation: AAH39138.1.
AF057286 mRNA. Translation: AAC97476.1. Different initiation.
CCDSiCCDS24816.1. [Q8CHU3-1]
CCDS56779.1. [Q8CHU3-2]
RefSeqiNP_001239118.1. NM_001252189.1. [Q8CHU3-2]
NP_034278.1. NM_010148.3. [Q8CHU3-1]
UniGeneiMm.139695.
Mm.489800.

Genome annotation databases

EnsembliENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036. [Q8CHU3-1]
ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036. [Q8CHU3-2]
ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036. [Q8CHU3-1]
GeneIDi13855.
KEGGimmu:13855.
UCSCiuc007jhv.2. mouse. [Q8CHU3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK036331 mRNA. Translation: BAC29387.1 .
AL604029 Genomic DNA. Translation: CAI24192.1 .
BC039138 mRNA. Translation: AAH39138.1 .
AF057286 mRNA. Translation: AAC97476.1 . Different initiation.
CCDSi CCDS24816.1. [Q8CHU3-1 ]
CCDS56779.1. [Q8CHU3-2 ]
RefSeqi NP_001239118.1. NM_001252189.1. [Q8CHU3-2 ]
NP_034278.1. NM_010148.3. [Q8CHU3-1 ]
UniGenei Mm.139695.
Mm.489800.

3D structure databases

ProteinModelPortali Q8CHU3.
SMRi Q8CHU3. Positions 1-144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199486. 3 interactions.
IntActi Q8CHU3. 3 interactions.
MINTi MINT-93098.
STRINGi 10090.ENSMUSP00000104352.

PTM databases

PhosphoSitei Q8CHU3.

Proteomic databases

MaxQBi Q8CHU3.
PaxDbi Q8CHU3.
PRIDEi Q8CHU3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001063 ; ENSMUSP00000001063 ; ENSMUSG00000001036 . [Q8CHU3-1 ]
ENSMUST00000108713 ; ENSMUSP00000104353 ; ENSMUSG00000001036 . [Q8CHU3-2 ]
ENSMUST00000178202 ; ENSMUSP00000136553 ; ENSMUSG00000001036 . [Q8CHU3-1 ]
GeneIDi 13855.
KEGGi mmu:13855.
UCSCi uc007jhv.2. mouse. [Q8CHU3-1 ]

Organism-specific databases

CTDi 22905.
MGIi MGI:1333766. Epn2.

Phylogenomic databases

eggNOGi NOG263730.
GeneTreei ENSGT00550000074611.
HOGENOMi HOG000008298.
HOVERGENi HBG006690.
KOi K12471.
PhylomeDBi Q8CHU3.
TreeFami TF313361.

Miscellaneous databases

ChiTaRSi EPN2. mouse.
NextBioi 284722.
PROi Q8CHU3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8CHU3.
Bgeei Q8CHU3.
CleanExi MM_EPN2.
Genevestigatori Q8CHU3.

Family and domain databases

Gene3Di 1.25.40.90. 1 hit.
InterProi IPR008942. ENTH_VHS.
IPR027319. Epsin-2_metazoa.
IPR013809. Epsin-like_N.
IPR001026. Epsin_dom_N.
IPR003903. Ubiquitin-int_motif.
[Graphical view ]
PANTHERi PTHR12276:SF50. PTHR12276:SF50. 1 hit.
Pfami PF01417. ENTH. 1 hit.
[Graphical view ]
SMARTi SM00273. ENTH. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view ]
SUPFAMi SSF48464. SSF48464. 1 hit.
PROSITEi PS50942. ENTH. 1 hit.
PS50330. UIM. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Intersectin, a novel adaptor protein with two eps15 homology and five src homology 3 domains."
    Yamabhai M., Hoffman N.G., Hardison N.L., McPherson P.S., Castagnoli L., Cesareni G., Kay B.K.
    J. Biol. Chem. 273:31401-31407(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-595 (ISOFORM 2), INTERACTION WITH ITSN1.
  5. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 77-86, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.

Entry informationi

Entry nameiEPN2_MOUSE
AccessioniPrimary (citable) accession number: Q8CHU3
Secondary accession number(s): O70447, Q5NCM4, Q8BZ85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi