Reviewed,
UniProtKB/Swiss-Prot Q8CHT0 (AL4A1_MOUSE)
Last modified
June 16, 2009.
Version 62.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Short name=P5C dehydrogenase EC=1.5.1.12 Alternative name(s): Aldehyde dehydrogenase family 4 member A1 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 562 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes By similarity. |
| Catalytic activity | (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Mitochondrion matrix By similarity. |
| Post-translational modification | Acetylation of Lys-98, Lys-113 and Lys-401 is observed in liver mitochondria from fasted mice but not from fed mice. |
| Sequence similarities | Belongs to the aldehyde dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Proline metabolism |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Acetylation Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic processInferred from electronic annotation. Source: InterPro |
| Cellular component | mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 23 | 23 | Mitochondrion By similarity | ||||||
| Chain | 24 – 562 | 539 | Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial | PRO_0000007174 | |||||
Regions | |||||||||
| Nucleotide binding | 295 – 300 | 6 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 313 | 1 | By similarity | ||||||
| Active site | 347 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 98 | 1 | N6-acetyllysine Ref.3 | ||||||
| Modified residue | 113 | 1 | N6-acetyllysine Ref.3 | ||||||
| Modified residue | 401 | 1 | N6-acetyllysine Ref.3 | ||||||
| Modified residue | 504 | 1 | Phosphotyrosine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 47 | 1 | D → G in BAC32045. Ref.1 | ||||||
Sequences
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References
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Retina. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney and Liver. |
| [3] | "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-98; LYS-113 AND LYS-401, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AK044712 mRNA. Translation: BAC32045.1. BC024133 mRNA. Translation: AAH24133.1. BC026589 mRNA. Translation: AAH26589.1. BC039281 mRNA. Translation: AAH39281.2. BC056226 mRNA. Translation: AAH56226.1. | |
| IPI | IPI00405699. |
| UniGene | Mm.273571 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q8CHT0. |
Proteomic databases | |
| PRIDE | Q8CHT0. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000028737. Mus musculus. [Contig view] |
| KEGG | mmu:212647. |
Organism-specific databases | |
| MGI | MGI:2443883. Aldh4a1. |
Phylogenomic databases | |
| HOGENOM | Q8CHT0. |
| HOVERGEN | Q8CHT0. |
Enzyme and pathway databases | |
| BRENDA | 1.5.1.12. 244. |
Gene expression databases | |
| ArrayExpress | Q8CHT0. |
| Bgee | Q8CHT0. |
| CleanEx | MM_ALDH4A1. |
| GermOnline | ENSMUSG00000028737. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005931. d-1-pyrroline-5-COlate_DH-1. [Graphical view] |
| Gene3D | G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| PANTHER | PTHR11699. Aldehyde_dehyd. 1 hit. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01236. D1pyr5carbox1. 1 hit. |
| PROSITE | PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 373656. |
| SOURCE | Search... |
Entry information
| Entry name | AL4A1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8CHT0 Secondary accession number(s): Q7TND0 Q8R1S2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


