Reviewed, UniProtKB/Swiss-Prot Q8CHR6 (DPYD_MOUSE)

Names and origin

Protein names

Recommended name:
    Dihydropyrimidine dehydrogenase [NADP+]
      Short name=DHPDHase
      Short name=DPD
    EC=1.3.1.2
Alternative name(s):
    Dihydrouracil dehydrogenase
    Dihydrothymine dehydrogenase

Gene names

Name:

Dpyd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine By similarity.
Catalytic activity	5,6-dihydrouracil + NADP(+) = uracil + NADPH.
Cofactor	Binds 2 4Fe-4S clusters By similarity. FAD By similarity. FMN By similarity.
Enzyme regulation	Inactivated by 5-iodouracil By similarity.
Pathway	Amino-acid biosynthesis; beta-alanine biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the dihydropyrimidine dehydrogenase family. Contains 3 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	4Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW thymidine catabolic process Inferred from sequence or structural similarity. Source: UniProtKB uracil catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from direct assay. Source: MGI
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro dihydropyrimidine dehydrogenase (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1025	1025	Dihydropyrimidine dehydrogenase [NADP+]		PRO_0000327501
Regions
	Domain	69 – 100	32	4Fe-4S ferredoxin-type 1
	Domain	944 – 976	33	4Fe-4S ferredoxin-type 2
	Domain	978 – 1007	30	4Fe-4S ferredoxin-type 3
Sites
	Metal binding	953	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	956	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	959	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	963	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	986	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	989	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	992	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	996	1	Iron-sulfur 2 (4Fe-4S) Potential
Amino acid modifications
	Modified residue	577	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CHR6-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: AA1D1B3E3B72C504	FASTA	1,025	111,253
	10 20 30 40 50 60 MAGVLSRDAP DIESILALNP RVQAHATLRS TAAKKLDKKH WKRNTDKNCF TCEKLESNFD 70 80 90 100 110 120 DIKHTTLGER GALREAVRCL KCADAPCQKS CPTSLDIKSF ITSIANKNYY GAAKLIFSDN 130 140 150 160 170 180 PLGLTCGMVC PTSDLCVGGC NLHAAEEGPI NIGGLQQFAT EVFKAMNIPQ IRNPSLPPPE 190 200 210 220 230 240 HMPEAYSAKI ALFGAGPASI SCASFLARLG YSNITIFEKQ EYVGGLSTSE IPQFRLPYDV 250 260 270 280 290 300 VNFEIELMKD LGVKIICGKS LSTDEMTLSS LKENGYRAAF IGIGLPEPKK DHIFQGLTQV 310 320 330 340 350 360 QGFYTSKDFL PLVAKSSKTG MCACHSPLPS IRGAVIVLGA GDTAFDCATS ALRCGALRVF 370 380 390 400 410 420 IVFRKGFVNI RAVPEEMELA KEEKCEFLPF LSPRKVIVKD GKIVAMQFVR TEQDETGNWV 430 440 450 460 470 480 EDEEQTVRLK ADVVISAFGS VLEDPKVKEA LSPIKFNRWG LPEVNPETMQ TSEPWVFAGG 490 500 510 520 530 540 DVVGMANTTV ESVNDGKQAS WYIHKHIQAQ YGTSVPSQPT MPLFYTPVDL VDISVEMAGL 550 560 570 580 590 600 RFPNPFGLAS ATPATSTPMI RRAFEAGWGF ALTKTFSLDK DIVTNVSPRI IRGTTSGPLY 610 620 630 640 650 660 GPGQSSFLNI ELISEKTAAY WCHSVTELKA DFPDNILIAS IMCSYNKSDW MELSKMAEAS 670 680 690 700 710 720 GADALELNLS CPHGMGERGM GLACGQDPEL VRNICRWVRQ AVRVPFFAKL TPNVTDIVSI 730 740 750 760 770 780 ARAAKEGGAD GVTATNTVSG LMGLKADGTP WPAVGIGRRT TYGGVSGTAI RPIALRAVTA 790 800 810 820 830 840 IARALPGFPI LATGGIDSAE SGLQFLHSGA SVLQVCSAIQ NQDFTVIEDY CTGLKALLYL 850 860 870 880 890 900 KSIEELADWD GQSPPIISHQ KGKPVPRVAE LMGQKLPSFG PYLEQRKKII AASKIRQKDQ 910 920 930 940 950 960 NTACSPLQRK HFNSQKPIPA IKDVIGKSLQ YLGTFGEMSI MEQVVALIDE EMCINCGKCY 970 980 990 1000 1010 1020 MTCNDSGYQA IQFDPETHLP TVSDTCTGCT LCLSVCPIMD CIRMVSRATP YQPKRGLPLA VKPVC

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC039699 mRNA. Translation: AAH39699.1.
RefSeq	NP_740748.1.
UniGene	Mm.27907
3D structure databases
HSSP	HSSP built from PDB template 1GTE based on UniProtKB Q28943.
SMR	Q8CHR6. Positions 2-1017.
ModBase	Search...
PTM databases
PhosphoSite	Q8CHR6.
Genome annotation databases
Ensembl	ENSMUSG00000033308. Mus musculus. [Contig view]
GeneID	99586.
KEGG	mmu:99586.
Organism-specific databases
MGI	MGI:2139667. Dpyd.
Phylogenomic databases
HOGENOM	Q8CHR6.
HOVERGEN	Q8CHR6.
Gene expression databases
ArrayExpress	Q8CHR6.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR000759. Adrndx_reductase. IPR013785. Aldolase_TIM. IPR005720. DHO_DHase_1_core. IPR001295. Dihydroorotate_DHase_core. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR012285. Fum_reductase_C. IPR016040. NAD(P)-bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. PF00037. Fer4. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN. PR00419. ADXRDTASE. PR00368. FADPNR. PR00469. PNDRDTASEII.
TIGRFAMs	TIGR01037. pyrD_sub1_fam. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	354029.
SOURCE	Search...

Entry information

Entry name

DPYD_MOUSE

Accession

Primary (citable) accession number: Q8CHR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2003
Last modified:	November 25, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)