Dihydropyrimidine dehydrogenase [NADP+]

Preferred order of sections

Names and origin

Protein names

Recommended name:
Dihydropyrimidine dehydrogenase [NADP+]
Short name=DHPDHase
Short name=DPD
EC=1.3.1.2

Alternative name(s):
Dihydrothymine dehydrogenase
Dihydrouracil dehydrogenase

Gene names

Name:

Dpyd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine By similarity.
Catalytic activity	5,6-dihydrouracil + NADP⁺ = uracil + NADPH.
Cofactor	Binds 2 4Fe-4S clusters By similarity. FAD By similarity. FMN By similarity.
Enzyme regulation	Inactivated by 5-iodouracil By similarity.
Pathway	Amino-acid biosynthesis; beta-alanine biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the dihydropyrimidine dehydrogenase family. Contains 3 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	4Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro thymidine catabolic process Inferred from sequence or structural similarity. Source: UniProtKB uracil catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from direct assay. Source: MGI
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro dihydropyrimidine dehydrogenase (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1025

1025

Dihydropyrimidine dehydrogenase [NADP+]

PRO_0000327501

Regions

Domain

69 – 100

32

4Fe-4S ferredoxin-type 1

Domain

944 – 976

33

4Fe-4S ferredoxin-type 2

Domain

978 – 1007

30

4Fe-4S ferredoxin-type 3

Sites

Metal binding

953

1

Iron-sulfur 1 (4Fe-4S) Potential

Metal binding

956

1

Iron-sulfur 1 (4Fe-4S) Potential

Metal binding

959

1

Iron-sulfur 1 (4Fe-4S) Potential

Metal binding

963

1

Iron-sulfur 1 (4Fe-4S) Potential

Metal binding

986

1

Iron-sulfur 2 (4Fe-4S) Potential

Metal binding

989

1

Iron-sulfur 2 (4Fe-4S) Potential

Metal binding

992

1

Iron-sulfur 2 (4Fe-4S) Potential

Metal binding

996

1

Iron-sulfur 2 (4Fe-4S) Potential

Amino acid modifications

Modified residue

384

1

N6-acetyllysine By similarity

Modified residue

577

1

Phosphoserine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CHR6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: AA1D1B3E3B72C504
Show »

FASTA

1,025

111,253

        10         20         30         40         50         60 
MAGVLSRDAP DIESILALNP RVQAHATLRS TAAKKLDKKH WKRNTDKNCF TCEKLESNFD 

        70         80         90        100        110        120 
DIKHTTLGER GALREAVRCL KCADAPCQKS CPTSLDIKSF ITSIANKNYY GAAKLIFSDN 

       130        140        150        160        170        180 
PLGLTCGMVC PTSDLCVGGC NLHAAEEGPI NIGGLQQFAT EVFKAMNIPQ IRNPSLPPPE 

       190        200        210        220        230        240 
HMPEAYSAKI ALFGAGPASI SCASFLARLG YSNITIFEKQ EYVGGLSTSE IPQFRLPYDV 

       250        260        270        280        290        300 
VNFEIELMKD LGVKIICGKS LSTDEMTLSS LKENGYRAAF IGIGLPEPKK DHIFQGLTQV 

       310        320        330        340        350        360 
QGFYTSKDFL PLVAKSSKTG MCACHSPLPS IRGAVIVLGA GDTAFDCATS ALRCGALRVF 

       370        380        390        400        410        420 
IVFRKGFVNI RAVPEEMELA KEEKCEFLPF LSPRKVIVKD GKIVAMQFVR TEQDETGNWV 

       430        440        450        460        470        480 
EDEEQTVRLK ADVVISAFGS VLEDPKVKEA LSPIKFNRWG LPEVNPETMQ TSEPWVFAGG 

       490        500        510        520        530        540 
DVVGMANTTV ESVNDGKQAS WYIHKHIQAQ YGTSVPSQPT MPLFYTPVDL VDISVEMAGL 

       550        560        570        580        590        600 
RFPNPFGLAS ATPATSTPMI RRAFEAGWGF ALTKTFSLDK DIVTNVSPRI IRGTTSGPLY 

       610        620        630        640        650        660 
GPGQSSFLNI ELISEKTAAY WCHSVTELKA DFPDNILIAS IMCSYNKSDW MELSKMAEAS 

       670        680        690        700        710        720 
GADALELNLS CPHGMGERGM GLACGQDPEL VRNICRWVRQ AVRVPFFAKL TPNVTDIVSI 

       730        740        750        760        770        780 
ARAAKEGGAD GVTATNTVSG LMGLKADGTP WPAVGIGRRT TYGGVSGTAI RPIALRAVTA 

       790        800        810        820        830        840 
IARALPGFPI LATGGIDSAE SGLQFLHSGA SVLQVCSAIQ NQDFTVIEDY CTGLKALLYL 

       850        860        870        880        890        900 
KSIEELADWD GQSPPIISHQ KGKPVPRVAE LMGQKLPSFG PYLEQRKKII AASKIRQKDQ 

       910        920        930        940        950        960 
NTACSPLQRK HFNSQKPIPA IKDVIGKSLQ YLGTFGEMSI MEQVVALIDE EMCINCGKCY 

       970        980        990       1000       1010       1020 
MTCNDSGYQA IQFDPETHLP TVSDTCTGCT LCLSVCPIMD CIRMVSRATP YQPKRGLPLA 


VKPVC

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References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC039699 mRNA. Translation: AAH39699.1.
IPI	IPI00229705.
RefSeq	NP_740748.1. NM_170778.2.
UniGene	Mm.27907.
3D structure databases
HSSP	HSSP built from PDB template 1GTE based on UniProtKB Q28943.
ProteinModelPortal	Q8CHR6.
SMR	Q8CHR6. Positions 2-1019.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CHR6.
PTM databases
PhosphoSite	Q8CHR6.
Proteomic databases
PRIDE	Q8CHR6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000039177; ENSMUSP00000039429; ENSMUSG00000033308.
GeneID	99586.
KEGG	mmu:99586.
UCSC	uc008rdh.1. mouse.
Organism-specific databases
CTD	1806.
MGI	MGI:2139667. Dpyd.
Phylogenomic databases
HOGENOM	HBG314257.
HOVERGEN	HBG004351.
InParanoid	Q8CHR6.
OMA	AYWCQSV.
OrthoDB	EOG44J2H8.
PhylomeDB	Q8CHR6.
Gene expression databases
ArrayExpress	Q8CHR6.
Bgee	Q8CHR6.
Genevestigator	Q8CHR6.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR013785. Aldolase_TIM. IPR005720. Dihydroorotate_DH. IPR012135. Dihydroorotate_DH_1_2. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
KO	K00207.
Pfam	PF01180. DHO_dh. 1 hit. PF12838. Fer4_7. 1 hit. [Graphical view]
SUPFAM	SSF46548. Helical_ferredxn. 1 hit.
TIGRFAMs	TIGR01037. PyrD_sub1_fam. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	354029.
SOURCE	Search...

Entry information

Entry name

DPYD_MOUSE

Accession

Primary (citable) accession number: Q8CHR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents