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Protein

Dihydropyrimidine dehydrogenase [NADP(+)]

Gene

Dpyd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine (By similarity).By similarity

Catalytic activityi

5,6-dihydrouracil + NADP+ = uracil + NADPH.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters. Contains approximately 16 iron atoms per subunit.By similarity
  • FADBy similarity
  • FMNBy similarity

Enzyme regulationi

Inactivated by 5-iodouracil.By similarity

Pathwayi: beta-alanine biosynthesis

This protein is involved in the pathway beta-alanine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway beta-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi87Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi91Iron-sulfur 2 (4Fe-4S)By similarity1
Binding sitei129FAD; via carbonyl oxygenBy similarity1
Metal bindingi130Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi136Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi140Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi156Iron-sulfur 2 (4Fe-4S)By similarity1
Binding sitei235FADBy similarity1
Binding sitei261FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei371NADPBy similarity1
Binding sitei550FMNBy similarity1
Binding sitei609SubstrateBy similarity1
Active sitei671Proton acceptorBy similarity1
Binding sitei709FMNBy similarity1
Binding sitei767FMN; via amide nitrogenBy similarity1
Metal bindingi953Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi956Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi959Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi963Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi986Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi989Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi992Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi996Iron-sulfur 4 (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi194 – 198FADBy similarity5
Nucleotide bindingi218 – 226FADBy similarity9
Nucleotide bindingi340 – 343NADPBy similarity4
Nucleotide bindingi364 – 365NADPBy similarity2
Nucleotide bindingi437 – 439NADPBy similarity3
Nucleotide bindingi480 – 489FADBy similarity10
Nucleotide bindingi481 – 487NADPBy similarity7
Nucleotide bindingi574 – 575FMNBy similarity2
Nucleotide bindingi793 – 795FMNBy similarity3
Nucleotide bindingi816 – 817FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73621. Pyrimidine catabolism.
UniPathwayiUPA00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropyrimidine dehydrogenase [NADP(+)] (EC:1.3.1.2)
Short name:
DHPDHase
Short name:
DPD
Alternative name(s):
Dihydrothymine dehydrogenase
Dihydrouracil dehydrogenase
Gene namesi
Name:Dpyd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2139667. Dpyd.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003275011 – 1025Dihydropyrimidine dehydrogenase [NADP(+)]Add BLAST1025

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei384N6-acetyllysineBy similarity1
Modified residuei905PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8CHR6.
PaxDbiQ8CHR6.
PeptideAtlasiQ8CHR6.
PRIDEiQ8CHR6.

PTM databases

iPTMnetiQ8CHR6.
PhosphoSitePlusiQ8CHR6.
SwissPalmiQ8CHR6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033308.
ExpressionAtlasiQ8CHR6. baseline and differential.
GenevisibleiQ8CHR6. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8CHR6. 2 interactors.
MINTiMINT-4116112.
STRINGi10090.ENSMUSP00000039429.

Structurei

3D structure databases

ProteinModelPortaliQ8CHR6.
SMRiQ8CHR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 1004Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST32
Domaini944 – 9764Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST33
Domaini978 – 10074Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni668 – 670Substrate bindingBy similarity3
Regioni736 – 737Substrate bindingBy similarity2

Sequence similaritiesi

Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0399. Eukaryota.
COG0167. LUCA.
COG0493. LUCA.
COG1146. LUCA.
GeneTreeiENSGT00500000044896.
HOGENOMiHOG000007797.
HOVERGENiHBG004351.
InParanoidiQ8CHR6.
KOiK00207.
OMAiYQAIEFD.
OrthoDBiEOG091G00YL.
PhylomeDBiQ8CHR6.
TreeFamiTF105791.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.50.50.60. 3 hits.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
PF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR01037. pyrD_sub1_fam. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CHR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVLSRDAP DIESILALNP RVQAHATLRS TAAKKLDKKH WKRNTDKNCF
60 70 80 90 100
TCEKLESNFD DIKHTTLGER GALREAVRCL KCADAPCQKS CPTSLDIKSF
110 120 130 140 150
ITSIANKNYY GAAKLIFSDN PLGLTCGMVC PTSDLCVGGC NLHAAEEGPI
160 170 180 190 200
NIGGLQQFAT EVFKAMNIPQ IRNPSLPPPE HMPEAYSAKI ALFGAGPASI
210 220 230 240 250
SCASFLARLG YSNITIFEKQ EYVGGLSTSE IPQFRLPYDV VNFEIELMKD
260 270 280 290 300
LGVKIICGKS LSTDEMTLSS LKENGYRAAF IGIGLPEPKK DHIFQGLTQV
310 320 330 340 350
QGFYTSKDFL PLVAKSSKTG MCACHSPLPS IRGAVIVLGA GDTAFDCATS
360 370 380 390 400
ALRCGALRVF IVFRKGFVNI RAVPEEMELA KEEKCEFLPF LSPRKVIVKD
410 420 430 440 450
GKIVAMQFVR TEQDETGNWV EDEEQTVRLK ADVVISAFGS VLEDPKVKEA
460 470 480 490 500
LSPIKFNRWG LPEVNPETMQ TSEPWVFAGG DVVGMANTTV ESVNDGKQAS
510 520 530 540 550
WYIHKHIQAQ YGTSVPSQPT MPLFYTPVDL VDISVEMAGL RFPNPFGLAS
560 570 580 590 600
ATPATSTPMI RRAFEAGWGF ALTKTFSLDK DIVTNVSPRI IRGTTSGPLY
610 620 630 640 650
GPGQSSFLNI ELISEKTAAY WCHSVTELKA DFPDNILIAS IMCSYNKSDW
660 670 680 690 700
MELSKMAEAS GADALELNLS CPHGMGERGM GLACGQDPEL VRNICRWVRQ
710 720 730 740 750
AVRVPFFAKL TPNVTDIVSI ARAAKEGGAD GVTATNTVSG LMGLKADGTP
760 770 780 790 800
WPAVGIGRRT TYGGVSGTAI RPIALRAVTA IARALPGFPI LATGGIDSAE
810 820 830 840 850
SGLQFLHSGA SVLQVCSAIQ NQDFTVIEDY CTGLKALLYL KSIEELADWD
860 870 880 890 900
GQSPPIISHQ KGKPVPRVAE LMGQKLPSFG PYLEQRKKII AASKIRQKDQ
910 920 930 940 950
NTACSPLQRK HFNSQKPIPA IKDVIGKSLQ YLGTFGEMSI MEQVVALIDE
960 970 980 990 1000
EMCINCGKCY MTCNDSGYQA IQFDPETHLP TVSDTCTGCT LCLSVCPIMD
1010 1020
CIRMVSRATP YQPKRGLPLA VKPVC
Length:1,025
Mass (Da):111,253
Last modified:March 1, 2003 - v1
Checksum:iAA1D1B3E3B72C504
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039699 mRNA. Translation: AAH39699.1.
CCDSiCCDS17797.1.
RefSeqiNP_740748.1. NM_170778.2.
UniGeneiMm.27907.

Genome annotation databases

EnsembliENSMUST00000039177; ENSMUSP00000039429; ENSMUSG00000033308.
GeneIDi99586.
KEGGimmu:99586.
UCSCiuc008rdh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039699 mRNA. Translation: AAH39699.1.
CCDSiCCDS17797.1.
RefSeqiNP_740748.1. NM_170778.2.
UniGeneiMm.27907.

3D structure databases

ProteinModelPortaliQ8CHR6.
SMRiQ8CHR6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CHR6. 2 interactors.
MINTiMINT-4116112.
STRINGi10090.ENSMUSP00000039429.

PTM databases

iPTMnetiQ8CHR6.
PhosphoSitePlusiQ8CHR6.
SwissPalmiQ8CHR6.

Proteomic databases

MaxQBiQ8CHR6.
PaxDbiQ8CHR6.
PeptideAtlasiQ8CHR6.
PRIDEiQ8CHR6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039177; ENSMUSP00000039429; ENSMUSG00000033308.
GeneIDi99586.
KEGGimmu:99586.
UCSCiuc008rdh.1. mouse.

Organism-specific databases

CTDi1806.
MGIiMGI:2139667. Dpyd.

Phylogenomic databases

eggNOGiKOG0399. Eukaryota.
COG0167. LUCA.
COG0493. LUCA.
COG1146. LUCA.
GeneTreeiENSGT00500000044896.
HOGENOMiHOG000007797.
HOVERGENiHBG004351.
InParanoidiQ8CHR6.
KOiK00207.
OMAiYQAIEFD.
OrthoDBiEOG091G00YL.
PhylomeDBiQ8CHR6.
TreeFamiTF105791.

Enzyme and pathway databases

UniPathwayiUPA00131.
ReactomeiR-MMU-73621. Pyrimidine catabolism.

Miscellaneous databases

ChiTaRSiDpyd. mouse.
PROiQ8CHR6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033308.
ExpressionAtlasiQ8CHR6. baseline and differential.
GenevisibleiQ8CHR6. MM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.50.50.60. 3 hits.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR028261. DPD_II.
IPR023753. FAD/NAD-binding_dom.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
PF14691. Fer4_20. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR01037. pyrD_sub1_fam. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPYD_MOUSE
AccessioniPrimary (citable) accession number: Q8CHR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.