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Q8CHQ0

- FBX4_MOUSE

UniProt

Q8CHQ0 - FBX4_MOUSE

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Protein
F-box only protein 4
Gene
Fbxo4, Fbx4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1 By similarity.3 Publications

Pathwayi

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. ubiquitin-protein transferase activity Source: Ensembl

GO - Biological processi

  1. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. positive regulation of protein ubiquitination Source: UniProtKB
  3. protein polyubiquitination Source: UniProtKB
  4. telomere maintenance Source: UniProtKB
  5. ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 4
Gene namesi
Name:Fbxo4
Synonyms:Fbx4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:2146220. Fbxo4.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. SCF ubiquitin ligase complex Source: UniProtKB
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385F-box only protein 4
PRO_0000119880Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Ser-11 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase. Phosphorylation at Ser-11 is important for homodimerization and for optimal ubiquitin ligase activity towards CCND1.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CHQ0.
PRIDEiQ8CHQ0.

PTM databases

PhosphoSiteiQ8CHQ0.

Expressioni

Gene expression databases

BgeeiQ8CHQ0.
CleanExiMM_FBXO4.
GenevestigatoriQ8CHQ0.

Interactioni

Subunit structurei

Homodimer. Directly interacts with SKP1 and CUL1. Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO4) formed of CUL1, SKP1, RBX1 and FBXO4. Interacts with TERF1; this interaction is prevented in the presence of GNL3L. Identified in a complex with CRYAB and CCND1 By similarity.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YwhaeP622592EBI-3895153,EBI-356480

Protein-protein interaction databases

BioGridi222983. 3 interactions.
IntActiQ8CHQ0. 1 interaction.
STRINGi10090.ENSMUSP00000022791.

Structurei

3D structure databases

ProteinModelPortaliQ8CHQ0.
SMRiQ8CHQ0. Positions 55-382.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 10047F-box
Add
BLAST

Sequence similaritiesi

Contains 1 F-box domain.

Phylogenomic databases

eggNOGiNOG39270.
GeneTreeiENSGT00390000014416.
HOGENOMiHOG000112550.
HOVERGENiHBG051585.
InParanoidiQ8CHQ0.
KOiK10291.
OMAiHEWQDEF.
OrthoDBiEOG7GBG00.
TreeFamiTF331105.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CHQ0-1 [UniParc]FASTAAdd to Basket

« Hide

MAGSEPRGAG SPPPASDWGR LEAAILSGWR TFWYSVAKER ATPTASRKEA    50
AEETSALTRL PVDVQLYILS FLSPHDLCQL GSTDHYWNKT IRDPILWRYF 100
LLRDLPSWSS VDWKSLPDLE ILKKPISEVT DSTCLDYMEV YKMCCPYTRR 150
ALKASRPMYG VVTSFLHSLI IQNEPRFAMF GPGLEELNTS LVLSLMSSED 200
LCPTAGLPHR QIDGIGSGVN FQLNNQQKFN ILILYSTTRK ERDRAREEHT 250
STVNKMFSLQ SEGDEQQGSR YSVIPQIQKV CEVVDGFIYV ANAEAHRRHE 300
WQDEFSRIMA MTDPAFGSSG RPMLVLSCIS QADVKRMPCF YLAHELHLSL 350
LNHPWMVQDT EAETLTGFLN GIEWILEEVE SKRAK 385
Length:385
Mass (Da):43,777
Last modified:July 27, 2011 - v2
Checksum:i36D78194EAF9EE95
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911I → V in AAH40086. 1 Publication
Sequence conflicti347 – 3471H → R in AAH40086. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC137902 Genomic DNA. No translation available.
BC040086 mRNA. Translation: AAH40086.1.
AJ300659 mRNA. Translation: CAC36404.1.
CCDSiCCDS27359.1.
RefSeqiNP_598860.2. NM_134099.2.
UniGeneiMm.234191.

Genome annotation databases

EnsembliENSMUST00000022791; ENSMUSP00000022791; ENSMUSG00000022184.
GeneIDi106052.
KEGGimmu:106052.
UCSCiuc007vcf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC137902 Genomic DNA. No translation available.
BC040086 mRNA. Translation: AAH40086.1 .
AJ300659 mRNA. Translation: CAC36404.1 .
CCDSi CCDS27359.1.
RefSeqi NP_598860.2. NM_134099.2.
UniGenei Mm.234191.

3D structure databases

ProteinModelPortali Q8CHQ0.
SMRi Q8CHQ0. Positions 55-382.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 222983. 3 interactions.
IntActi Q8CHQ0. 1 interaction.
STRINGi 10090.ENSMUSP00000022791.

PTM databases

PhosphoSitei Q8CHQ0.

Proteomic databases

PaxDbi Q8CHQ0.
PRIDEi Q8CHQ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022791 ; ENSMUSP00000022791 ; ENSMUSG00000022184 .
GeneIDi 106052.
KEGGi mmu:106052.
UCSCi uc007vcf.2. mouse.

Organism-specific databases

CTDi 26272.
MGIi MGI:2146220. Fbxo4.

Phylogenomic databases

eggNOGi NOG39270.
GeneTreei ENSGT00390000014416.
HOGENOMi HOG000112550.
HOVERGENi HBG051585.
InParanoidi Q8CHQ0.
KOi K10291.
OMAi HEWQDEF.
OrthoDBi EOG7GBG00.
TreeFami TF331105.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi 358036.
PROi Q8CHQ0.
SOURCEi Search...

Gene expression databases

Bgeei Q8CHQ0.
CleanExi MM_FBXO4.
Genevestigatori Q8CHQ0.

Family and domain databases

InterProi IPR001810. F-box_dom.
[Graphical view ]
Pfami PF12937. F-box-like. 1 hit.
[Graphical view ]
SMARTi SM00256. FBOX. 1 hit.
[Graphical view ]
SUPFAMi SSF81383. SSF81383. 1 hit.
PROSITEi PS50181. FBOX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  3. "Osteoblasts express the mouse F-box protein mFbx4."
    Kopecky B.S., Varga F., Klaushofer K.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 245-385.
    Tissue: Osteoblast.
  4. "Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex."
    Lin D.I., Barbash O., Kumar K.G., Weber J.D., Harper J.W., Klein-Szanto A.J., Rustgi A., Fuchs S.Y., Diehl J.A.
    Mol. Cell 24:355-366(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A SCF UBIQUITIN-PROTEIN LIGASE COMPLEX, SUBUNIT, SUBCELLULAR LOCATION.
  5. "Mutations in Fbx4 inhibit dimerization of the SCF(Fbx4) ligase and contribute to cyclin D1 overexpression in human cancer."
    Barbash O., Zamfirova P., Lin D.I., Chen X., Yang K., Nakagawa H., Lu F., Rustgi A.K., Diehl J.A.
    Cancer Cell 14:68-78(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-11.
  6. "GNL3L stabilizes the TRF1 complex and promotes mitotic transition."
    Zhu Q., Meng L., Hsu J.K., Lin T., Teishima J., Tsai R.Y.
    J. Cell Biol. 185:827-839(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TERF1.
  7. "Lysine 269 is essential for cyclin D1 ubiquitylation by the SCF(Fbx4/alphaB-crystallin) ligase and subsequent proteasome-dependent degradation."
    Barbash O., Egan E., Pontano L.L., Kosak J., Diehl J.A.
    Oncogene 28:4317-4325(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiFBX4_MOUSE
AccessioniPrimary (citable) accession number: Q8CHQ0
Secondary accession number(s): E9QPM9, Q99JG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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