Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8CHQ0

- FBX4_MOUSE

UniProt

Q8CHQ0 - FBX4_MOUSE

Protein

F-box only protein 4

Gene

Fbxo4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1 By similarity.By similarity

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin-protein transferase activity Source: Ensembl

    GO - Biological processi

    1. positive regulation of protein ubiquitination Source: UniProtKB
    2. protein polyubiquitination Source: UniProtKB
    3. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    4. telomere maintenance Source: UniProtKB
    5. ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    F-box only protein 4
    Gene namesi
    Name:Fbxo4
    Synonyms:Fbx4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:2146220. Fbxo4.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. SCF ubiquitin ligase complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385F-box only protein 4PRO_0000119880Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation at Ser-11 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase. Phosphorylation at Ser-11 is important for homodimerization and for optimal ubiquitin ligase activity towards CCND1.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8CHQ0.
    PRIDEiQ8CHQ0.

    PTM databases

    PhosphoSiteiQ8CHQ0.

    Expressioni

    Gene expression databases

    BgeeiQ8CHQ0.
    CleanExiMM_FBXO4.
    GenevestigatoriQ8CHQ0.

    Interactioni

    Subunit structurei

    Homodimer. Directly interacts with SKP1 and CUL1. Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO4) formed of CUL1, SKP1, RBX1 and FBXO4. Interacts with TERF1; this interaction is prevented in the presence of GNL3L. Identified in a complex with CRYAB and CCND1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    YwhaeP622592EBI-3895153,EBI-356480

    Protein-protein interaction databases

    BioGridi222983. 3 interactions.
    IntActiQ8CHQ0. 1 interaction.
    STRINGi10090.ENSMUSP00000022791.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CHQ0.
    SMRiQ8CHQ0. Positions 55-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 10047F-boxPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 F-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG39270.
    GeneTreeiENSGT00390000014416.
    HOGENOMiHOG000112550.
    HOVERGENiHBG051585.
    InParanoidiQ8CHQ0.
    KOiK10291.
    OMAiHEWQDEF.
    OrthoDBiEOG7GBG00.
    TreeFamiTF331105.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    [Graphical view]
    PfamiPF12937. F-box-like. 1 hit.
    [Graphical view]
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF81383. SSF81383. 1 hit.
    PROSITEiPS50181. FBOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CHQ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGSEPRGAG SPPPASDWGR LEAAILSGWR TFWYSVAKER ATPTASRKEA    50
    AEETSALTRL PVDVQLYILS FLSPHDLCQL GSTDHYWNKT IRDPILWRYF 100
    LLRDLPSWSS VDWKSLPDLE ILKKPISEVT DSTCLDYMEV YKMCCPYTRR 150
    ALKASRPMYG VVTSFLHSLI IQNEPRFAMF GPGLEELNTS LVLSLMSSED 200
    LCPTAGLPHR QIDGIGSGVN FQLNNQQKFN ILILYSTTRK ERDRAREEHT 250
    STVNKMFSLQ SEGDEQQGSR YSVIPQIQKV CEVVDGFIYV ANAEAHRRHE 300
    WQDEFSRIMA MTDPAFGSSG RPMLVLSCIS QADVKRMPCF YLAHELHLSL 350
    LNHPWMVQDT EAETLTGFLN GIEWILEEVE SKRAK 385
    Length:385
    Mass (Da):43,777
    Last modified:July 27, 2011 - v2
    Checksum:i36D78194EAF9EE95
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti91 – 911I → V in AAH40086. (PubMed:15489334)Curated
    Sequence conflicti347 – 3471H → R in AAH40086. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137902 Genomic DNA. No translation available.
    BC040086 mRNA. Translation: AAH40086.1.
    AJ300659 mRNA. Translation: CAC36404.1.
    CCDSiCCDS27359.1.
    RefSeqiNP_598860.2. NM_134099.2.
    UniGeneiMm.234191.

    Genome annotation databases

    EnsembliENSMUST00000022791; ENSMUSP00000022791; ENSMUSG00000022184.
    GeneIDi106052.
    KEGGimmu:106052.
    UCSCiuc007vcf.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC137902 Genomic DNA. No translation available.
    BC040086 mRNA. Translation: AAH40086.1 .
    AJ300659 mRNA. Translation: CAC36404.1 .
    CCDSi CCDS27359.1.
    RefSeqi NP_598860.2. NM_134099.2.
    UniGenei Mm.234191.

    3D structure databases

    ProteinModelPortali Q8CHQ0.
    SMRi Q8CHQ0. Positions 55-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 222983. 3 interactions.
    IntActi Q8CHQ0. 1 interaction.
    STRINGi 10090.ENSMUSP00000022791.

    PTM databases

    PhosphoSitei Q8CHQ0.

    Proteomic databases

    PaxDbi Q8CHQ0.
    PRIDEi Q8CHQ0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022791 ; ENSMUSP00000022791 ; ENSMUSG00000022184 .
    GeneIDi 106052.
    KEGGi mmu:106052.
    UCSCi uc007vcf.2. mouse.

    Organism-specific databases

    CTDi 26272.
    MGIi MGI:2146220. Fbxo4.

    Phylogenomic databases

    eggNOGi NOG39270.
    GeneTreei ENSGT00390000014416.
    HOGENOMi HOG000112550.
    HOVERGENi HBG051585.
    InParanoidi Q8CHQ0.
    KOi K10291.
    OMAi HEWQDEF.
    OrthoDBi EOG7GBG00.
    TreeFami TF331105.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_199115. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    NextBioi 358036.
    PROi Q8CHQ0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CHQ0.
    CleanExi MM_FBXO4.
    Genevestigatori Q8CHQ0.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    [Graphical view ]
    Pfami PF12937. F-box-like. 1 hit.
    [Graphical view ]
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81383. SSF81383. 1 hit.
    PROSITEi PS50181. FBOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.
    3. "Osteoblasts express the mouse F-box protein mFbx4."
      Kopecky B.S., Varga F., Klaushofer K.
      Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 245-385.
      Tissue: Osteoblast.
    4. "Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex."
      Lin D.I., Barbash O., Kumar K.G., Weber J.D., Harper J.W., Klein-Szanto A.J., Rustgi A., Fuchs S.Y., Diehl J.A.
      Mol. Cell 24:355-366(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN A SCF UBIQUITIN-PROTEIN LIGASE COMPLEX, SUBUNIT, SUBCELLULAR LOCATION.
    5. "Mutations in Fbx4 inhibit dimerization of the SCF(Fbx4) ligase and contribute to cyclin D1 overexpression in human cancer."
      Barbash O., Zamfirova P., Lin D.I., Chen X., Yang K., Nakagawa H., Lu F., Rustgi A.K., Diehl J.A.
      Cancer Cell 14:68-78(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT SER-11.
    6. "GNL3L stabilizes the TRF1 complex and promotes mitotic transition."
      Zhu Q., Meng L., Hsu J.K., Lin T., Teishima J., Tsai R.Y.
      J. Cell Biol. 185:827-839(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TERF1.
    7. "Lysine 269 is essential for cyclin D1 ubiquitylation by the SCF(Fbx4/alphaB-crystallin) ligase and subsequent proteasome-dependent degradation."
      Barbash O., Egan E., Pontano L.L., Kosak J., Diehl J.A.
      Oncogene 28:4317-4325(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiFBX4_MOUSE
    AccessioniPrimary (citable) accession number: Q8CHQ0
    Secondary accession number(s): E9QPM9, Q99JG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3