F-box only protein 4 - Mus musculus (Mouse)

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Q8CHQ0 (FBX4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
F-box only protein 4

Gene names

Name:	Fbxo4
Synonyms:	Fbx4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1 By similarity. Ref.4 Ref.5 Ref.7
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Homodimer. Directly interacts with SKP1 and CUL1. Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO4) formed of CUL1, SKP1, RBX1 and FBXO4. Interacts with TERF1; this interaction is prevented in the presence of GNL3L. Identified in a complex with CRYAB and CCND1 By similarity. Ref.4 Ref.6
Subcellular location	Cytoplasm Ref.4.
Post-translational modification	Phosphorylation at Ser-11 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase. Phosphorylation at Ser-11 is important for homodimerization and for optimal ubiquitin ligase activity towards CCND1.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein polyubiquitination Inferred from sequence or structural similarity. Source: UniProtKB telomere maintenance Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	SCF ubiquitin ligase complex Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin-protein ligase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ywhae	P62259	2	EBI-3895153,EBI-356480

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 385

385

F-box only protein 4

PRO_0000119880

Regions

Domain

54 – 100

F-box

Amino acid modifications

Modified residue

Phosphoserine Ref.5

Experimental info

Sequence conflict

I → V in AAH40086. Ref.2

Sequence conflict

347

H → R in AAH40086. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CHQ0 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 36D78194EAF9EE95
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FASTA

385

43,777

        10         20         30         40         50         60 
MAGSEPRGAG SPPPASDWGR LEAAILSGWR TFWYSVAKER ATPTASRKEA AEETSALTRL 

        70         80         90        100        110        120 
PVDVQLYILS FLSPHDLCQL GSTDHYWNKT IRDPILWRYF LLRDLPSWSS VDWKSLPDLE 

       130        140        150        160        170        180 
ILKKPISEVT DSTCLDYMEV YKMCCPYTRR ALKASRPMYG VVTSFLHSLI IQNEPRFAMF 

       190        200        210        220        230        240 
GPGLEELNTS LVLSLMSSED LCPTAGLPHR QIDGIGSGVN FQLNNQQKFN ILILYSTTRK 

       250        260        270        280        290        300 
ERDRAREEHT STVNKMFSLQ SEGDEQQGSR YSVIPQIQKV CEVVDGFIYV ANAEAHRRHE 

       310        320        330        340        350        360 
WQDEFSRIMA MTDPAFGSSG RPMLVLSCIS QADVKRMPCF YLAHELHLSL LNHPWMVQDT 

       370        380 
EAETLTGFLN GIEWILEEVE SKRAK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
[3]	"Osteoblasts express the mouse F-box protein mFbx4." Kopecky B.S., Varga F., Klaushofer K. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 245-385. Tissue: Osteoblast.
[4]	"Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex." Lin D.I., Barbash O., Kumar K.G., Weber J.D., Harper J.W., Klein-Szanto A.J., Rustgi A., Fuchs S.Y., Diehl J.A. Mol. Cell 24:355-366(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A SCF UBIQUITIN-PROTEIN LIGASE COMPLEX, SUBUNIT, SUBCELLULAR LOCATION.
[5]	"Mutations in Fbx4 inhibit dimerization of the SCF(Fbx4) ligase and contribute to cyclin D1 overexpression in human cancer." Barbash O., Zamfirova P., Lin D.I., Chen X., Yang K., Nakagawa H., Lu F., Rustgi A.K., Diehl J.A. Cancer Cell 14:68-78(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT SER-11.
[6]	"GNL3L stabilizes the TRF1 complex and promotes mitotic transition." Zhu Q., Meng L., Hsu J.K., Lin T., Teishima J., Tsai R.Y. J. Cell Biol. 185:827-839(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TERF1.
[7]	"Lysine 269 is essential for cyclin D1 ubiquitylation by the SCF(Fbx4/alphaB-crystallin) ligase and subsequent proteasome-dependent degradation." Barbash O., Egan E., Pontano L.L., Kosak J., Diehl J.A. Oncogene 28:4317-4325(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC137902 Genomic DNA. No translation available. BC040086 mRNA. Translation: AAH40086.1. AJ300659 mRNA. Translation: CAC36404.1.
IPI	IPI00336840.
RefSeq	NP_598860.2. NM_134099.2.
UniGene	Mm.234191.
3D structure databases
ProteinModelPortal	Q8CHQ0.
SMR	Q8CHQ0. Positions 55-382.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CHQ0. 1 interaction.
STRING	10090.ENSMUSP00000022791.
PTM databases
PhosphoSite	Q8CHQ0.
Proteomic databases
PaxDb	Q8CHQ0.
PRIDE	Q8CHQ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022791; ENSMUSP00000022791; ENSMUSG00000022184.
GeneID	106052.
KEGG	mmu:106052.
UCSC	uc007vcf.2. mouse.
Organism-specific databases
CTD	26272.
MGI	MGI:2146220. Fbxo4.
Phylogenomic databases
eggNOG	NOG39270.
GeneTree	ENSGT00390000014416.
HOGENOM	HOG000112550.
HOVERGEN	HBG051585.
InParanoid	Q8CHQ0.
KO	K10291.
OMA	HEWQDEF.
OrthoDB	EOG4VQ9PC.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	Q8CHQ0.
CleanEx	MM_FBXO4.
Genevestigator	Q8CHQ0.
GermOnline	ENSMUSG00000022184. Mus musculus.
Family and domain databases
InterPro	IPR001810. F-box_dom_cyclin-like. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	358036.
SOURCE	Search...

Entry information

Entry name

FBX4_MOUSE

Accession

Primary (citable) accession number: Q8CHQ0
Secondary accession number(s): E9QPM9, Q99JG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents