Reviewed, UniProtKB/Swiss-Prot Q8CHN6 (SGPL1_RAT)

Names and origin

Protein names

Recommended name:
    Sphingosine-1-phosphate lyase 1
      Short name=SP-lyase 1
      Short name=SPL 1
      Short name=SPL
    EC=4.1.2.27
Alternative name(s):
    Sphingosine-1-phosphate aldolase

Gene names

Name:	Sgpl1
Synonyms:	Spl

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis By similarity.
Catalytic activity	Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.
Sequence similarities	Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.

Ontologies

Keywords
Biological process	Apoptosis Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Ligand	Pyridoxal phosphate
Molecular function	Lyase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	apoptosis Inferred from sequence or structural similarity. Source: UniProtKB carboxylic acid metabolic process Inferred from electronic annotation. Source: InterPro ceramide metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: InterPro pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro sphinganine-1-phosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Sphingosine-1-phosphate lyase 1		PRO_0000147014
Regions
	Topological domain	1 – 41	41	Lumenal Potential
	Transmembrane	42 – 62	21	Signal-anchor for type III membrane protein Potential
	Topological domain	63 – 568	506	Cytoplasmic Potential
Amino acid modifications
	Modified residue	353	1	N6-(pyridoxal phosphate)lysine By similarity
	Modified residue	353	1	N6-acetyllysine By similarity
	Modified residue	564	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CHN6-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A0B59A072CC79F48	FASTA	568	63,758
	10 20 30 40 50 60 MPSTDLLKLK DFEPYLEILE AYSTKAKNYV NGYCTKYEPW QLIAGSVLCT LLVVWVYELI 70 80 90 100 110 120 FQPESLWSRF KNKLFRLIRK MPFIGRKIQQ QLTKAKKDLV KNMPFLKLDK DYVKTLPAQG 130 140 150 160 170 180 LSTAEVLERL KEYSSMDVFW QEGKASGAVY SGEPKLTELL VQAYGEFTWS NPLHPDIFPG 190 200 210 220 230 240 LRKLEAEIVR MTCSLFNGGP DSCGCVTSGG TESILMACKA YRDLALEKGI KTPEIVAPES 250 260 270 280 290 300 AHAAFDKAAH YFGMKIVRVA QKKNMEVDVR AMKRAISRNT AMLVCSAPQF PHGVIDPIPE 310 320 330 340 350 360 VAKLAVKYKI PFHVDACLGG FLIVFMEKAG YPLEKPFDFR VKGVTSISAD THKYGYAPKG 370 380 390 400 410 420 SSVVMYSNEK YRKYQFFVDA DWQGGIYASP SIAGSRPGGI IAACWAALMH FGENGYVEAT 430 440 450 460 470 480 KQIIKTARFL KSELENIKNI FILGDPQLSV IALGSNDFDI YRLSNMMSAK GWNFNYLQFP 490 500 510 520 530 540 RSIHFCITLV HTRKRVAIQF LKDIRESVTQ IMKNPKAKTT GMGAIYGMAQ ATIDRKMVAE 550 560 ISSVFLDSLY STDPVTQGNQ MNGSPKPR

References

[1]	"Comparison of sphingosine-1-phosphate lyases." Van Veldhoven P.P. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ512838 mRNA. Translation: CAD55407.1.
IPI	IPI00193172.
RefSeq	NP_775139.1.
UniGene	Rn.26953
3D structure databases
SMR	Q8CHN6. Positions 81-506.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CHN6.
Proteomic databases
PRIDE	Q8CHN6.
Genome annotation databases
Ensembl	ENSRNOT00000065127; ENSRNOP00000059089; ENSRNOG00000000565; Rattus norvegicus. [Genome view]
GeneID	286896.
KEGG	rno:286896.
UCSC	NM_173116. rat.
Organism-specific databases
CTD	286896.
RGD	628599. Sgpl1.
Phylogenomic databases
eggNOG	roNOG05892.
HOVERGEN	Q8CHN6.
InParanoid	Q8CHN6.
PhylomeDB	Q8CHN6.
Enzyme and pathway databases
BRENDA	4.1.2.27. 248.
Gene expression databases
ArrayExpress	Q8CHN6.
Genevestigator	Q8CHN6.
GermOnline	ENSRNOG00000000565. Rattus norvegicus.
Family and domain databases
InterPro	IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	624977.

Entry information

Entry name

SGPL1_RAT

Accession

Primary (citable) accession number: Q8CHN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)