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Protein

2-hydroxyacyl-CoA lyase 1

Gene

Hacl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.

Catalytic activityi

2-hydroxyphytanoyl-CoA = pristanal + formyl-CoA.

Cofactori

Protein has several cofactor binding sites:

Kineticsi

  1. KM=15 µM for 2-hydroxy-3-methylhexadecanoyl-CoA1 Publication

    pH dependencei

    Optimum pH is 7.5-8.1 Publication

    Pathwayi: fatty acid metabolism

    This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei63Thiamine pyrophosphateBy similarity1
    Metal bindingi458MagnesiumBy similarity1
    Metal bindingi485MagnesiumBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • fatty acid alpha-oxidation Source: HGNC
    • lipid metabolic process Source: RGD
    • protein oligomerization Source: RGD

    Keywordsi

    Molecular functionLyase
    LigandMagnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17701
    ReactomeiR-RNO-389599 Alpha-oxidation of phytanate
    SABIO-RKiQ8CHM7
    UniPathwayiUPA00199

    Chemistry databases

    SwissLipidsiSLP:000001016

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-hydroxyacyl-CoA lyase 1 (EC:4.1.-.-)
    Alternative name(s):
    2-hydroxyphytanoyl-CoA lyase
    Short name:
    2-HPCL
    Phytanoyl-CoA 2-hydroxylase 2
    Gene namesi
    Name:Hacl1
    Synonyms:2hpcl, Hpcl2, Phyh2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 16

    Organism-specific databases

    RGDi619849 Hacl1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003376751 – 5812-hydroxyacyl-CoA lyase 1Add BLAST581

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2PhosphoserineCombined sources1
    Modified residuei4PhosphoserineCombined sources1
    Modified residuei6PhosphoserineCombined sources1
    Modified residuei361N6-succinyllysineBy similarity1
    Modified residuei368N6-succinyllysineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8CHM7
    PRIDEiQ8CHM7

    PTM databases

    iPTMnetiQ8CHM7
    PhosphoSitePlusiQ8CHM7

    Expressioni

    Gene expression databases

    BgeeiENSRNOG00000019630
    GenevisibleiQ8CHM7 RN

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    GO - Molecular functioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000026588

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CHM7
    SMRiQ8CHM7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni404 – 489Thiamine pyrophosphate bindingBy similarityAdd BLAST86

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi579 – 581Microbody targeting signalBy similarity3

    Sequence similaritiesi

    Belongs to the TPP enzyme family.Curated

    Phylogenomic databases

    eggNOGiKOG1185 Eukaryota
    COG0028 LUCA
    GeneTreeiENSGT00530000063412
    HOGENOMiHOG000053808
    HOVERGENiHBG027302
    InParanoidiQ8CHM7
    KOiK12261
    OMAiQGMVRQW
    OrthoDBiEOG091G03OS
    PhylomeDBiQ8CHM7
    TreeFamiTF105690

    Family and domain databases

    InterProiView protein in InterPro
    IPR029035 DHS-like_NAD/FAD-binding_dom
    IPR029061 THDP-binding
    IPR012000 Thiamin_PyroP_enz_cen_dom
    IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
    IPR011766 TPP_enzyme-bd_C
    PfamiView protein in Pfam
    PF02775 TPP_enzyme_C, 1 hit
    PF00205 TPP_enzyme_M, 1 hit
    PF02776 TPP_enzyme_N, 1 hit
    SUPFAMiSSF52467 SSF52467, 1 hit
    SSF52518 SSF52518, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q8CHM7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSESNSAEGS DRSEEQVSGA KVIAQALKTQ DVEYMFGVVG IPVTEIALAA
    60 70 80 90 100
    QELGIKYIGM RNEQAACYAA SAVGYLTGRP GVCLVVSGPG LIHALGGMAN
    110 120 130 140 150
    ANMNCWPLIV IGGSSERNQE AMGAFQEFPQ VEACRLYSKF SARPSSIQHI
    160 170 180 190 200
    PFVIEKAVRS SIYGRPGACY IDVPADLVTL QESTTSIKYK ECCMPPPLSL
    210 220 230 240 250
    AETSAVRAAA SVLRSAKQPL LIIGKGAAYS HAEDSIRKLV ELCNLPFLPT
    260 270 280 290 300
    PMGKGVVPDN HPNCVGAARS RALQFADVIV LFGARLNWIL HFGLPPRYQA
    310 320 330 340 350
    DVKFIQIDIC AEELGNNVRP SVTLLGDVNA VSKQLLEQFV KNPWQYPTDS
    360 370 380 390 400
    KWWETLREKR KNNEAVSKEL ASKKSLPMNY YTVFYHVQEQ LPRNCFIVSE
    410 420 430 440 450
    GANTMDIGRT VLQNYLPRHR LDAGSFGTMG VGLGFAIAAA VVAKERSPGQ
    460 470 480 490 500
    RVICVEGDSA FGFSGMEVET ICRYNLPIII LVVNNNGIYQ GFDADTWGKI
    510 520 530 540 550
    LNFQGTATTI PPMCLLPNSH YEQVMTAFGG KGYFVQTPEE LQDSLRQALK
    560 570 580
    DTSKPCLINI MIEPQSTRKA QDFHWLTRSN I
    Length:581
    Mass (Da):63,616
    Last modified:March 1, 2003 - v1
    Checksum:i007F0738173A8BA9
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BC078697 mRNA Translation: AAH78697.1
    AJ517469 mRNA Translation: CAD56981.1
    RefSeqiNP_445945.1, NM_053493.1
    UniGeneiRn.219233

    Genome annotation databases

    EnsembliENSRNOT00000026588; ENSRNOP00000026588; ENSRNOG00000019630
    GeneIDi85255
    KEGGirno:85255
    UCSCiRGD:619849 rat

    Similar proteinsi

    Entry informationi

    Entry nameiHACL1_RAT
    AccessioniPrimary (citable) accession number: Q8CHM7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 1, 2003
    Last modified: March 28, 2018
    This is version 106 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health