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Protein

2-hydroxyacyl-CoA lyase 1

Gene

Hacl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.

Catalytic activityi

2-hydroxyphytanoyl-CoA = pristanal + formyl-CoA.

Cofactori

Protein has several cofactor binding sites:

Kineticsi

  1. KM=15 µM for 2-hydroxy-3-methylhexadecanoyl-CoA1 Publication

    pH dependencei

    Optimum pH is 7.5-8.1 Publication

    Pathwayi: fatty acid metabolism

    This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei63Thiamine pyrophosphateBy similarity1
    Metal bindingi458MagnesiumBy similarity1
    Metal bindingi485MagnesiumBy similarity1

    GO - Molecular functioni

    • carbon-carbon lyase activity Source: HGNC
    • magnesium ion binding Source: InterPro
    • thiamine pyrophosphate binding Source: HGNC

    GO - Biological processi

    • fatty acid alpha-oxidation Source: HGNC
    • protein oligomerization Source: Ensembl
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17701.
    ReactomeiR-RNO-389599. Alpha-oxidation of phytanate.
    UniPathwayiUPA00199.

    Chemistry databases

    SwissLipidsiSLP:000001016.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-hydroxyacyl-CoA lyase 1 (EC:4.1.-.-)
    Alternative name(s):
    2-hydroxyphytanoyl-CoA lyase
    Short name:
    2-HPCL
    Phytanoyl-CoA 2-hydroxylase 2
    Gene namesi
    Name:Hacl1
    Synonyms:2hpcl, Hpcl2, Phyh2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 16

    Organism-specific databases

    RGDi619849. Hacl1.

    Subcellular locationi

    GO - Cellular componenti

    • peroxisome Source: HGNC
    Complete GO annotation...

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003376751 – 5812-hydroxyacyl-CoA lyase 1Add BLAST581

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2PhosphoserineCombined sources1
    Modified residuei4PhosphoserineCombined sources1
    Modified residuei6PhosphoserineCombined sources1
    Modified residuei361N6-succinyllysineBy similarity1
    Modified residuei368N6-succinyllysineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8CHM7.
    PRIDEiQ8CHM7.

    PTM databases

    iPTMnetiQ8CHM7.
    PhosphoSitePlusiQ8CHM7.

    Expressioni

    Gene expression databases

    BgeeiENSRNOG00000019630.
    GenevisibleiQ8CHM7. RN.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000026588.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CHM7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni404 – 489Thiamine pyrophosphate bindingBy similarityAdd BLAST86

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi579 – 581Microbody targeting signalBy similarity3

    Sequence similaritiesi

    Belongs to the TPP enzyme family.Curated

    Phylogenomic databases

    eggNOGiKOG1185. Eukaryota.
    COG0028. LUCA.
    GeneTreeiENSGT00530000063412.
    HOGENOMiHOG000053808.
    HOVERGENiHBG027302.
    InParanoidiQ8CHM7.
    KOiK12261.
    OMAiFRHEQHA.
    OrthoDBiEOG091G03OS.
    PhylomeDBiQ8CHM7.
    TreeFamiTF105690.

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q8CHM7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSESNSAEGS DRSEEQVSGA KVIAQALKTQ DVEYMFGVVG IPVTEIALAA
    60 70 80 90 100
    QELGIKYIGM RNEQAACYAA SAVGYLTGRP GVCLVVSGPG LIHALGGMAN
    110 120 130 140 150
    ANMNCWPLIV IGGSSERNQE AMGAFQEFPQ VEACRLYSKF SARPSSIQHI
    160 170 180 190 200
    PFVIEKAVRS SIYGRPGACY IDVPADLVTL QESTTSIKYK ECCMPPPLSL
    210 220 230 240 250
    AETSAVRAAA SVLRSAKQPL LIIGKGAAYS HAEDSIRKLV ELCNLPFLPT
    260 270 280 290 300
    PMGKGVVPDN HPNCVGAARS RALQFADVIV LFGARLNWIL HFGLPPRYQA
    310 320 330 340 350
    DVKFIQIDIC AEELGNNVRP SVTLLGDVNA VSKQLLEQFV KNPWQYPTDS
    360 370 380 390 400
    KWWETLREKR KNNEAVSKEL ASKKSLPMNY YTVFYHVQEQ LPRNCFIVSE
    410 420 430 440 450
    GANTMDIGRT VLQNYLPRHR LDAGSFGTMG VGLGFAIAAA VVAKERSPGQ
    460 470 480 490 500
    RVICVEGDSA FGFSGMEVET ICRYNLPIII LVVNNNGIYQ GFDADTWGKI
    510 520 530 540 550
    LNFQGTATTI PPMCLLPNSH YEQVMTAFGG KGYFVQTPEE LQDSLRQALK
    560 570 580
    DTSKPCLINI MIEPQSTRKA QDFHWLTRSN I
    Length:581
    Mass (Da):63,616
    Last modified:March 1, 2003 - v1
    Checksum:i007F0738173A8BA9
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BC078697 mRNA. Translation: AAH78697.1.
    AJ517469 mRNA. Translation: CAD56981.1.
    RefSeqiNP_445945.1. NM_053493.1.
    UniGeneiRn.219233.

    Genome annotation databases

    EnsembliENSRNOT00000026588; ENSRNOP00000026588; ENSRNOG00000019630.
    GeneIDi85255.
    KEGGirno:85255.
    UCSCiRGD:619849. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BC078697 mRNA. Translation: AAH78697.1.
    AJ517469 mRNA. Translation: CAD56981.1.
    RefSeqiNP_445945.1. NM_053493.1.
    UniGeneiRn.219233.

    3D structure databases

    ProteinModelPortaliQ8CHM7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000026588.

    Chemistry databases

    SwissLipidsiSLP:000001016.

    PTM databases

    iPTMnetiQ8CHM7.
    PhosphoSitePlusiQ8CHM7.

    Proteomic databases

    PaxDbiQ8CHM7.
    PRIDEiQ8CHM7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000026588; ENSRNOP00000026588; ENSRNOG00000019630.
    GeneIDi85255.
    KEGGirno:85255.
    UCSCiRGD:619849. rat.

    Organism-specific databases

    CTDi26061.
    RGDi619849. Hacl1.

    Phylogenomic databases

    eggNOGiKOG1185. Eukaryota.
    COG0028. LUCA.
    GeneTreeiENSGT00530000063412.
    HOGENOMiHOG000053808.
    HOVERGENiHBG027302.
    InParanoidiQ8CHM7.
    KOiK12261.
    OMAiFRHEQHA.
    OrthoDBiEOG091G03OS.
    PhylomeDBiQ8CHM7.
    TreeFamiTF105690.

    Enzyme and pathway databases

    UniPathwayiUPA00199.
    BioCyciMetaCyc:MONOMER-17701.
    ReactomeiR-RNO-389599. Alpha-oxidation of phytanate.

    Miscellaneous databases

    PROiQ8CHM7.

    Gene expression databases

    BgeeiENSRNOG00000019630.
    GenevisibleiQ8CHM7. RN.

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHACL1_RAT
    AccessioniPrimary (citable) accession number: Q8CHM7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 1, 2003
    Last modified: November 2, 2016
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.