Reviewed, UniProtKB/Swiss-Prot Q8CHL0 (FZD5_RAT)

Names and origin

Protein names

Recommended name:
    Frizzled-5
      Short name=Fz-5

Gene names

Name:

Fzd5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway By similarity.
Subunit structure	Interacts with GOPC By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Golgi apparatus membrane; Multi-pass membrane protein By similarity. Note: Localized at the plasma membrane and also found at the Golgi By similarity.
Domain	The PDZ-binding motif mediates interaction with GOPC By similarity. Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity. The FZ domain is involved in binding with Wnt ligands By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell membrane Golgi apparatus Membrane
Domain	Signal Transmembrane
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW non-G-protein coupled 7TM receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 585	559	Frizzled-5		PRO_0000243937
Regions
	Topological domain	27 – 238	212	Extracellular Potential
	Transmembrane	239 – 259	21	1 Potential
	Topological domain	260 – 270	11	Cytoplasmic Potential
	Transmembrane	271 – 291	21	2 Potential
	Topological domain	292 – 315	24	Extracellular Potential
	Transmembrane	316 – 336	21	3 Potential
	Topological domain	337 – 358	22	Cytoplasmic Potential
	Transmembrane	359 – 379	21	4 Potential
	Topological domain	380 – 402	23	Extracellular Potential
	Transmembrane	403 – 423	21	5 Potential
	Topological domain	424 – 449	26	Cytoplasmic Potential
	Transmembrane	450 – 470	21	6 Potential
	Topological domain	471 – 500	30	Extracellular Potential
	Transmembrane	501 – 521	21	7 Potential
	Topological domain	522 – 585	64	Cytoplasmic Potential
	Domain	28 – 150	123	FZ
	Motif	525 – 530	6	Lys-Thr-X-X-X-Trp motif
	Motif	583 – 585	3	PDZ-binding By similarity
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33 ↔ 94		By similarity
	Disulfide bond	41 ↔ 87		By similarity
	Disulfide bond	78 ↔ 116		By similarity
	Disulfide bond	105 ↔ 147		By similarity
	Disulfide bond	109 ↔ 133		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CHL0-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BF26772B29FA12C8	FASTA	585	64,112
	10 20 30 40 50 60 MARPDPSAPP SLLLLLLAQL VGRAAAASKA PVCQEITVPM CRGIGYNLTH MPNQFNHDTQ 70 80 90 100 110 120 DEAGLEVHQF WPLVEIHCSP DLRFFLCSMY TPICLPDYHK PLPPCRSVCE RAKAGCSPLM 130 140 150 160 170 180 RQYGFAWPER MSCDRLPVLG GDAEVLCMDY NRSEATTASP KSFPAKPTLP GPPGAPSSGG 190 200 210 220 230 240 ECPSGGPSVC TCREPFVPIL KESHPLYNKV RTGQVPNCAV PCYQPSFSPD ERTFATFWIG 250 260 270 280 290 300 LWSVLCFIST STTVATFLID MERFRYPERP IIFLSACYLC VSLGFLVRLV VGHASVACSR 310 320 330 340 350 360 EHSHIHYETT GPALCTVVFL LVYFFGMASS IWWVILSLTW FLAAGMKWGN EAIAGYAQYF 370 380 390 400 410 420 HLAAWLIPSV KSITALALSS VDGDPVAGVC YVGNQNLNSL RGFVLGPLVL YLLVGTLFLL 430 440 450 460 470 480 AGFVSLFRIR SVIKQGGTKT DKLEKLMIRI GIFTLLYTVP ASIVVACYLY EQHYRESWEA 490 500 510 520 530 540 ALTCACPGSD AGQPRAKPEY WVLMLKYFMC LVVGITSGVW IWSGKTLESW RRFTSRCCCS 550 560 570 580 SRRGHKSGGA MVAGDYAEAS AALTGRTGPP GPAAAYHKQV SLSHV

References

[1]	"Molecular cloning and characterization of a gene encoding for rat Frizzled 5." Ito S., Imamura T., Shiota K. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB052909 mRNA. Translation: BAC53980.1.
IPI	IPI00193129.
RefSeq	NP_776210.1.
UniGene	Rn.24792
3D structure databases
HSSP	HSSP built from PDB template 1IJY based on UniProtKB Q61091.
SMR	Q8CHL0. Positions 33-152.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q8CHL0.
Genome annotation databases
GeneID	317674.
KEGG	rno:317674.
UCSC	NM_173838. rat.
Organism-specific databases
CTD	317674.
RGD	631406. Fzd5.
Phylogenomic databases
HOVERGEN	Q8CHL0.
Gene expression databases
Genevestigator	Q8CHL0.
Family and domain databases
InterPro	IPR000539. Frizzled. IPR020067. Frizzled-like_dom. IPR000024. Frizzled_Cys-rich. IPR020068. Frizzled_dom_subgr. IPR015526. Frizzled_related. IPR017981. GPCR_2-like. IPR017983. GPCR_2_secretin-like_CS. [Graphical view]
Gene3D	G3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHER	PTHR11309. Fz_related. 1 hit.
Pfam	PF01534. Frizzled. 1 hit. PF01392. Fz. 1 hit. [Graphical view]
PRINTS	PR00489. FRIZZLED.
SMART	SM00063. FRI. 1 hit. [Graphical view]
PROSITE	PS50038. FZ. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. False negative. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	672065.

Entry information

Entry name

FZD5_RAT

Accession

Primary (citable) accession number: Q8CHL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 1, 2003
Last modified:	October 13, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)