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Protein

Frizzled-5

Gene

Fzd5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway (By similarity).By similarity

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: GO_Central
  2. Wnt-activated receptor activity Source: GO_Central
  3. Wnt-protein binding Source: RGD

GO - Biological processi

  1. canonical Wnt signaling pathway Source: GO_Central
  2. multicellular organismal development Source: UniProtKB-KW
  3. non-canonical Wnt signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-5
Short name:
Fz-5
Gene namesi
Name:Fzd5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi631406. Fzd5.

Subcellular locationi

  1. Cell membrane By similarity; Multi-pass membrane protein By similarity
  2. Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity

  3. Note: Localized at the plasma membrane and also found at the Golgi.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 238212ExtracellularSequence AnalysisAdd
BLAST
Transmembranei239 – 25921Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini260 – 27011CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei271 – 29121Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini292 – 31524ExtracellularSequence AnalysisAdd
BLAST
Transmembranei316 – 33621Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini337 – 35822CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei359 – 37921Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini380 – 40223ExtracellularSequence AnalysisAdd
BLAST
Transmembranei403 – 42321Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini424 – 44926CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei450 – 47021Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini471 – 50030ExtracellularSequence AnalysisAdd
BLAST
Transmembranei501 – 52121Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini522 – 58564CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: GO_Central
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 585559Frizzled-5PRO_0000243937Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 94PROSITE-ProRule annotation
Disulfide bondi41 ↔ 87PROSITE-ProRule annotation
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi78 ↔ 116PROSITE-ProRule annotation
Disulfide bondi105 ↔ 147PROSITE-ProRule annotation
Disulfide bondi109 ↔ 133PROSITE-ProRule annotation
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8CHL0.

PTM databases

PhosphoSiteiQ8CHL0.

Expressioni

Gene expression databases

GenevestigatoriQ8CHL0.

Interactioni

Subunit structurei

Interacts with GOPC.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062259.

Structurei

3D structure databases

ProteinModelPortaliQ8CHL0.
SMRiQ8CHL0. Positions 33-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 150123FZPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi525 – 5306Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family membersBy similarity
Motifi583 – 5853PDZ-bindingBy similarity

Domaini

The PDZ-binding motif mediates interaction with GOPC.By similarity
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.By similarity
The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257258.
HOGENOMiHOG000233236.
HOVERGENiHBG006977.
InParanoidiQ8CHL0.
KOiK02375.
PhylomeDBiQ8CHL0.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026547. FZD5.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF29. PTHR11309:SF29. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CHL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARPDPSAPP SLLLLLLAQL VGRAAAASKA PVCQEITVPM CRGIGYNLTH
60 70 80 90 100
MPNQFNHDTQ DEAGLEVHQF WPLVEIHCSP DLRFFLCSMY TPICLPDYHK
110 120 130 140 150
PLPPCRSVCE RAKAGCSPLM RQYGFAWPER MSCDRLPVLG GDAEVLCMDY
160 170 180 190 200
NRSEATTASP KSFPAKPTLP GPPGAPSSGG ECPSGGPSVC TCREPFVPIL
210 220 230 240 250
KESHPLYNKV RTGQVPNCAV PCYQPSFSPD ERTFATFWIG LWSVLCFIST
260 270 280 290 300
STTVATFLID MERFRYPERP IIFLSACYLC VSLGFLVRLV VGHASVACSR
310 320 330 340 350
EHSHIHYETT GPALCTVVFL LVYFFGMASS IWWVILSLTW FLAAGMKWGN
360 370 380 390 400
EAIAGYAQYF HLAAWLIPSV KSITALALSS VDGDPVAGVC YVGNQNLNSL
410 420 430 440 450
RGFVLGPLVL YLLVGTLFLL AGFVSLFRIR SVIKQGGTKT DKLEKLMIRI
460 470 480 490 500
GIFTLLYTVP ASIVVACYLY EQHYRESWEA ALTCACPGSD AGQPRAKPEY
510 520 530 540 550
WVLMLKYFMC LVVGITSGVW IWSGKTLESW RRFTSRCCCS SRRGHKSGGA
560 570 580
MVAGDYAEAS AALTGRTGPP GPAAAYHKQV SLSHV
Length:585
Mass (Da):64,112
Last modified:March 1, 2003 - v1
Checksum:iBF26772B29FA12C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052909 mRNA. Translation: BAC53980.1.
RefSeqiNP_776210.1. NM_173838.1.
UniGeneiRn.24792.

Genome annotation databases

GeneIDi317674.
KEGGirno:317674.
UCSCiRGD:631406. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052909 mRNA. Translation: BAC53980.1.
RefSeqiNP_776210.1. NM_173838.1.
UniGeneiRn.24792.

3D structure databases

ProteinModelPortaliQ8CHL0.
SMRiQ8CHL0. Positions 33-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062259.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ8CHL0.

Proteomic databases

PaxDbiQ8CHL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi317674.
KEGGirno:317674.
UCSCiRGD:631406. rat.

Organism-specific databases

CTDi7855.
RGDi631406. Fzd5.

Phylogenomic databases

eggNOGiNOG257258.
HOGENOMiHOG000233236.
HOVERGENiHBG006977.
InParanoidiQ8CHL0.
KOiK02375.
PhylomeDBiQ8CHL0.

Miscellaneous databases

NextBioi672065.
PROiQ8CHL0.

Gene expression databases

GenevestigatoriQ8CHL0.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026547. FZD5.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF29. PTHR11309:SF29. 1 hit.
PfamiPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSiPR00489. FRIZZLED.
SMARTiSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a gene encoding for rat Frizzled 5."
    Ito S., Imamura T., Shiota K.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiFZD5_RAT
AccessioniPrimary (citable) accession number: Q8CHL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: February 4, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.