Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8CHL0 (FZD5_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Frizzled-5

Short name=Fz-5
Gene names
Name:Fzd5
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length585 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway By similarity.

Subunit structure

Interacts with GOPC By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Golgi apparatus membrane; Multi-pass membrane protein By similarity. Note: Localized at the plasma membrane and also found at the Golgi By similarity.

Domain

The PDZ-binding motif mediates interaction with GOPC By similarity.

Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway By similarity.

The FZ domain is involved in binding with Wnt ligands By similarity.

Post-translational modification

Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor Fz/Smo family.

Contains 1 FZ (frizzled) domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCell membrane
Golgi apparatus
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionDevelopmental protein
G-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell differentiation in thymus

Inferred from Biological aspect of Ancestor. Source: RefGenome

angiogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

apoptotic process

Inferred from Biological aspect of Ancestor. Source: RefGenome

axonogenesis

Inferred from Biological aspect of Ancestor. Source: RefGenome

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

canonical Wnt signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

embryonic camera-type eye development

Inferred from Biological aspect of Ancestor. Source: RefGenome

gonad development

Inferred from Biological aspect of Ancestor. Source: RefGenome

labyrinthine layer blood vessel development

Inferred from Biological aspect of Ancestor. Source: RefGenome

post-embryonic camera-type eye development

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell projection

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

PDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from physical interaction PubMed 18567805. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 585559Frizzled-5
PRO_0000243937

Regions

Topological domain27 – 238212Extracellular Potential
Transmembrane239 – 25921Helical; Name=1; Potential
Topological domain260 – 27011Cytoplasmic Potential
Transmembrane271 – 29121Helical; Name=2; Potential
Topological domain292 – 31524Extracellular Potential
Transmembrane316 – 33621Helical; Name=3; Potential
Topological domain337 – 35822Cytoplasmic Potential
Transmembrane359 – 37921Helical; Name=4; Potential
Topological domain380 – 40223Extracellular Potential
Transmembrane403 – 42321Helical; Name=5; Potential
Topological domain424 – 44926Cytoplasmic Potential
Transmembrane450 – 47021Helical; Name=6; Potential
Topological domain471 – 50030Extracellular Potential
Transmembrane501 – 52121Helical; Name=7; Potential
Topological domain522 – 58564Cytoplasmic Potential
Domain28 – 150123FZ
Motif525 – 5306Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members By similarity
Motif583 – 5853PDZ-binding By similarity

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation1511N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 94 By similarity
Disulfide bond41 ↔ 87 By similarity
Disulfide bond78 ↔ 116 By similarity
Disulfide bond105 ↔ 147 By similarity
Disulfide bond109 ↔ 133 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CHL0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: BF26772B29FA12C8

FASTA58564,112
        10         20         30         40         50         60 
MARPDPSAPP SLLLLLLAQL VGRAAAASKA PVCQEITVPM CRGIGYNLTH MPNQFNHDTQ 

        70         80         90        100        110        120 
DEAGLEVHQF WPLVEIHCSP DLRFFLCSMY TPICLPDYHK PLPPCRSVCE RAKAGCSPLM 

       130        140        150        160        170        180 
RQYGFAWPER MSCDRLPVLG GDAEVLCMDY NRSEATTASP KSFPAKPTLP GPPGAPSSGG 

       190        200        210        220        230        240 
ECPSGGPSVC TCREPFVPIL KESHPLYNKV RTGQVPNCAV PCYQPSFSPD ERTFATFWIG 

       250        260        270        280        290        300 
LWSVLCFIST STTVATFLID MERFRYPERP IIFLSACYLC VSLGFLVRLV VGHASVACSR 

       310        320        330        340        350        360 
EHSHIHYETT GPALCTVVFL LVYFFGMASS IWWVILSLTW FLAAGMKWGN EAIAGYAQYF 

       370        380        390        400        410        420 
HLAAWLIPSV KSITALALSS VDGDPVAGVC YVGNQNLNSL RGFVLGPLVL YLLVGTLFLL 

       430        440        450        460        470        480 
AGFVSLFRIR SVIKQGGTKT DKLEKLMIRI GIFTLLYTVP ASIVVACYLY EQHYRESWEA 

       490        500        510        520        530        540 
ALTCACPGSD AGQPRAKPEY WVLMLKYFMC LVVGITSGVW IWSGKTLESW RRFTSRCCCS 

       550        560        570        580 
SRRGHKSGGA MVAGDYAEAS AALTGRTGPP GPAAAYHKQV SLSHV 

« Hide

References

[1]"Molecular cloning and characterization of a gene encoding for rat Frizzled 5."
Ito S., Imamura T., Shiota K.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB052909 mRNA. Translation: BAC53980.1.
RefSeqNP_776210.1. NM_173838.1.
UniGeneRn.24792.

3D structure databases

ProteinModelPortalQ8CHL0.
SMRQ8CHL0. Positions 33-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000062259.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ8CHL0.

Proteomic databases

PaxDbQ8CHL0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID317674.
KEGGrno:317674.
UCSCRGD:631406. rat.

Organism-specific databases

CTD7855.
RGD631406. Fzd5.

Phylogenomic databases

eggNOGNOG257258.
HOGENOMHOG000233236.
HOVERGENHBG006977.
KOK02375.
PhylomeDBQ8CHL0.

Gene expression databases

GenevestigatorQ8CHL0.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR000539. Frizzled.
IPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR026547. FZD5.
IPR017981. GPCR_2-like.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF29. PTHR11309:SF29. 1 hit.
PfamPF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
[Graphical view]
PRINTSPR00489. FRIZZLED.
SMARTSM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio672065.
PROQ8CHL0.

Entry information

Entry nameFZD5_RAT
AccessionPrimary (citable) accession number: Q8CHL0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries