Q8CHK2 (REL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Relaxin-3

Alternative name(s):
Insulin-like peptide INSL7
Short name=Insulin-like peptide 7
Prorelaxin M3

Cleaved into the following 2 chains:

1. Relaxin-3 B chain
2. Relaxin-3 A chain

Gene names

Name:	Rln3
Synonyms:	Insl7

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in neuropeptide signaling processes. Ligand for LGR7, relaxin-3 receptor-1 and relaxin-3 receptor-2 By similarity.
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds.
Subcellular location	Secreted.
Tissue specificity	High expression in the brain localized to the pons/medulla with highest levels in pars ventromedialis of the dorsal tegmental nucleus. Significant expression is also detected in the spleen, thymus, lung, testis and ovary. Ref.2
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of cAMP biosynthetic process Inferred from sequence alignment Ref.2. Source: MGI
Cellular_component	extracellular region Traceable author statement Ref.2. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	By similarity
	Peptide	25 – 51	27	Relaxin-3 B chain By similarity		PRO_0000016085
	Propeptide	54 – 117	64	Connecting peptide By similarity		PRO_0000016086
	Peptide	118 – 141	24	Relaxin-3 A chain By similarity		PRO_0000016087
Amino acid modifications
	Disulfide bond	34 ↔ 128		Interchain (between B and A chains) By similarity
	Disulfide bond	46 ↔ 141		Interchain (between B and A chains) By similarity
	Disulfide bond	127 ↔ 132		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CHK2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0899CD9F57552951	FASTA	141	14,926
	10 20 30 40 50 60 MAMLGLLLLA SWALLGALGL QAEARPAPYG VKLCGREFIR AVIFTCGGSR WRRADILAHE 70 80 90 100 110 120 SLGDFFADGE ANTDHLASEL DEAVGSSEWL ALTKSPQAFY GGRASWQGSP GVVRGSRDVL 130 140 AGLSSSCCEW GCSKSQISSL C

References

[1]	"Production of recombinant human relaxin 3 in AtT20 cells." Kizawa H., Nishi K., Ishibashi Y., Harada M., Asano T., Ito Y., Suzuki N., Hinuma S., Fujisawa Y., Onda H., Nishimura O., Fujino M. Regul. Pept. 113:79-84(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human relaxin gene 3 (H3) and the equivalent mouse relaxin (M3) gene. Novel members of the relaxin peptide family." Bathgate R.A.D., Samuel C.S., Burazin T.C.D., Layfield S., Claasz A.A., Reytomas I.G.T., Dawson N.F., Zhao C., Bond C., Summers R.J., Parry L.J., Wade J.D., Tregear G.W. J. Biol. Chem. 277:1148-1157(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB076565 mRNA. Translation: BAC53760.1.
IPI	IPI00229664.
RefSeq	NP_775276.1. NM_173184.1.
UniGene	Mm.261583.
3D structure databases
ProteinModelPortal	Q8CHK2.
SMR	Q8CHK2. Positions 25-51.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000051392.
PTM databases
PhosphoSite	Q8CHK2.
Proteomic databases
PRIDE	Q8CHK2.
Protocols and materials databases
DNASU	212108.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000061923; ENSMUSP00000051392; ENSMUSG00000045232.
GeneID	212108.
KEGG	mmu:212108.
UCSC	uc009mls.1. mouse.
Organism-specific databases
CTD	117579.
MGI	MGI:2158015. Rln3.
Phylogenomic databases
eggNOG	NOG45013.
GeneTree	ENSGT00510000048203.
HOGENOM	HOG000111888.
HOVERGEN	HBG045048.
InParanoid	Q8CHK2.
KO	K05255.
OMA	RPSWQGT.
OrthoDB	EOG4VHK7X.
Gene expression databases
Bgee	Q8CHK2.
CleanEx	MM_RLN3.
Genevestigator	Q8CHK2.
Family and domain databases
InterPro	IPR016179. Insulin-like. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR00276. INSULINFAMLY.
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	373450.
SOURCE	Search...

Entry information

Entry name

REL3_MOUSE

Accession

Primary (citable) accession number: Q8CHK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program