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Protein

Septin-8

Gene

Sept8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106GTP; via amide nitrogenBy similarity1
Binding sitei241GTP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei256GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi51 – 58GTPBy similarity8
Nucleotide bindingi187 – 195GTPBy similarity9

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Septin-8
Gene namesi
Name:Sept8
Synonyms:Kiaa0202
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:894310. Sept8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • cytoskeleton Source: UniProtKB-SubCell
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001735342 – 429Septin-8Add BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei10PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CHH9.
PaxDbiQ8CHH9.
PRIDEiQ8CHH9.

2D gel databases

UCD-2DPAGEQ8CHH9.

PTM databases

iPTMnetiQ8CHH9.
PhosphoSitePlusiQ8CHH9.
SwissPalmiQ8CHH9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000018398.
CleanExiMM_SEPT8.
ExpressionAtlasiQ8CHH9. baseline and differential.
GenevisibleiQ8CHH9. MM.

Interactioni

Subunit structurei

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with CDK14, SEPT4, SEPT5 and SEPT7 (By similarity).By similarity

Protein-protein interaction databases

BioGridi203177. 1 interactor.
IntActiQ8CHH9. 5 interactors.
MINTiMINT-4134214.
STRINGi10090.ENSMUSP00000112920.

Structurei

3D structure databases

ProteinModelPortaliQ8CHH9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 307Septin-type GAdd BLAST267

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili320 – 412Sequence analysisAdd BLAST93

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2655. Eukaryota.
COG5019. LUCA.
GeneTreeiENSGT00860000133721.
HOGENOMiHOG000233586.
HOVERGENiHBG065093.
InParanoidiQ8CHH9.
KOiK16939.
PhylomeDBiQ8CHH9.
TreeFamiTF101080.

Family and domain databases

CDDicd01850. CDC_Septin. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR030646. SEPT8.
IPR016491. Septin.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 1 hit.
PTHR18884:SF54. PTHR18884:SF54. 1 hit.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
PIRSFiPIRSF006698. Septin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CHH9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATDLERVS NAEPEPRSLS LGGHVGFDSL PDQLVSKSVT QGFSFNILCV
60 70 80 90 100
GETGIGKSTL MNTLFNTTFE TEEASHHEEC VRLRPQTYDL QESNVHLKLT
110 120 130 140 150
IVDAVGFGDQ INKDDSYRPI VDYIDAQFEN YLQEELKIRR SLFDYHDTRI
160 170 180 190 200
HVCLYFITPT GHSLKSLDLV TMKKLDSKVN IIPIIAKADT ISKSELHKFK
210 220 230 240 250
IKIMGELVSN GVQIYQFPTD DEAVAEINAV MNAHLPFAVV GSTEEVKVGN
260 270 280 290 300
KLVRARQYPW GVVQVENENH CDFVKLREML IRVNMEDLRE QTHSRHYELY
310 320 330 340 350
RRCKLEEMGF QDSDGDSQPF SLQETYEAKR KEFLSELQRK EEEMRQMFVN
360 370 380 390 400
KVKETELELK EKERELHEKF EHLKRIHQEE KRKVEEKRRE LEEETNAFNC
410 420
RKAAMEALQS QALHATSQQP LRKDKDKKN
Length:429
Mass (Da):49,812
Last modified:January 23, 2007 - v4
Checksum:i133485FF0E8D3300
GO
Isoform 2 (identifier: Q8CHH9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-429: N → KF

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:430
Mass (Da):49,974
Checksum:i69B44485FF0E8D33
GO

Sequence cautioni

The sequence AAH49819 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC41399 differs from that shown. Reason: Frameshift at position 37.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58S → A in BAC41399 (PubMed:14621295).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009645429N → KF in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093215 mRNA. Translation: BAC41399.1. Sequence problems.
AL596095 Genomic DNA. Translation: CAP19145.1.
BC049819 mRNA. Translation: AAH49819.1. Different initiation.
BC059248 mRNA. Translation: AAH59248.1.
AF179996 mRNA. Translation: AAG09408.1.
CCDSiCCDS48794.1. [Q8CHH9-2]
CCDS56768.1. [Q8CHH9-1]
RefSeqiNP_001239261.1. NM_001252332.1. [Q8CHH9-1]
NP_001239262.1. NM_001252333.1.
NP_149156.1. NM_033144.2. [Q8CHH9-2]
UniGeneiMm.274399.

Genome annotation databases

EnsembliENSMUST00000108987; ENSMUSP00000104615; ENSMUSG00000018398. [Q8CHH9-1]
ENSMUST00000117061; ENSMUSP00000112920; ENSMUSG00000018398. [Q8CHH9-2]
GeneIDi20362.
KEGGimmu:20362.
UCSCiuc007iwh.2. mouse. [Q8CHH9-1]
uc007iwj.2. mouse. [Q8CHH9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093215 mRNA. Translation: BAC41399.1. Sequence problems.
AL596095 Genomic DNA. Translation: CAP19145.1.
BC049819 mRNA. Translation: AAH49819.1. Different initiation.
BC059248 mRNA. Translation: AAH59248.1.
AF179996 mRNA. Translation: AAG09408.1.
CCDSiCCDS48794.1. [Q8CHH9-2]
CCDS56768.1. [Q8CHH9-1]
RefSeqiNP_001239261.1. NM_001252332.1. [Q8CHH9-1]
NP_001239262.1. NM_001252333.1.
NP_149156.1. NM_033144.2. [Q8CHH9-2]
UniGeneiMm.274399.

3D structure databases

ProteinModelPortaliQ8CHH9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203177. 1 interactor.
IntActiQ8CHH9. 5 interactors.
MINTiMINT-4134214.
STRINGi10090.ENSMUSP00000112920.

PTM databases

iPTMnetiQ8CHH9.
PhosphoSitePlusiQ8CHH9.
SwissPalmiQ8CHH9.

2D gel databases

UCD-2DPAGEQ8CHH9.

Proteomic databases

EPDiQ8CHH9.
PaxDbiQ8CHH9.
PRIDEiQ8CHH9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108987; ENSMUSP00000104615; ENSMUSG00000018398. [Q8CHH9-1]
ENSMUST00000117061; ENSMUSP00000112920; ENSMUSG00000018398. [Q8CHH9-2]
GeneIDi20362.
KEGGimmu:20362.
UCSCiuc007iwh.2. mouse. [Q8CHH9-1]
uc007iwj.2. mouse. [Q8CHH9-2]

Organism-specific databases

CTDi23176.
MGIiMGI:894310. Sept8.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2655. Eukaryota.
COG5019. LUCA.
GeneTreeiENSGT00860000133721.
HOGENOMiHOG000233586.
HOVERGENiHBG065093.
InParanoidiQ8CHH9.
KOiK16939.
PhylomeDBiQ8CHH9.
TreeFamiTF101080.

Miscellaneous databases

ChiTaRSiSept8. mouse.
PROiQ8CHH9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018398.
CleanExiMM_SEPT8.
ExpressionAtlasiQ8CHH9. baseline and differential.
GenevisibleiQ8CHH9. MM.

Family and domain databases

CDDicd01850. CDC_Septin. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR030646. SEPT8.
IPR016491. Septin.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 1 hit.
PTHR18884:SF54. PTHR18884:SF54. 1 hit.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
PIRSFiPIRSF006698. Septin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEPT8_MOUSE
AccessioniPrimary (citable) accession number: Q8CHH9
Secondary accession number(s): B1AQY7, Q80YC7, Q9ESF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.