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Protein

GRIP and coiled-coil domain-containing protein 2

Gene

Gcc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
GRIP and coiled-coil domain-containing protein 2
Alternative name(s):
185 kDa Golgi coiled-coil protein
Short name:
GCC185
Gene namesi
Name:Gcc2
Synonyms:Kiaa0336
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1917547. Gcc2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16791679GRIP and coiled-coil domain-containing protein 2PRO_0000190075Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei1474 – 14741PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CHG3.
MaxQBiQ8CHG3.
PaxDbiQ8CHG3.
PRIDEiQ8CHG3.

PTM databases

iPTMnetiQ8CHG3.
PhosphoSiteiQ8CHG3.

Expressioni

Gene expression databases

CleanExiMM_GCC2.

Interactioni

Subunit structurei

Homodimer. Interacts (via GRIP domain) with RAB6A (preferentially in its GTP-bound form). May interact (RAB6A-dependent) with ARL1; might be involved in GCC2 Golgi localization. Interacts (probably via GRIP domain) with RAB9A (preferentially in its GTP-bound form). Interacts with CLASP1 and CLASP2; recruits both proteins to membranes of the TGN. Interacts with STX16 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Wasf2Q8BH436EBI-643470,EBI-643162

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8CHG3. 1 interaction.
MINTiMINT-1748522.
STRINGi10090.ENSMUSP00000054033.

Structurei

3D structure databases

ProteinModelPortaliQ8CHG3.
SMRiQ8CHG3. Positions 1565-1602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1604 – 165451GRIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1569 – 1679111Mediates interaction with RAB9ABy similarityAdd
BLAST
Regioni1569 – 160840Mediates interaction with RAB6ABy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili31 – 16131583Sequence analysisAdd
BLAST

Domaini

Extended rod-like protein with coiled-coil domains.

Sequence similaritiesi

Contains 1 GRIP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IUCE. Eukaryota.
ENOG410Y42S. LUCA.
HOVERGENiHBG045522.
InParanoidiQ8CHG3.

Family and domain databases

Gene3Di1.10.220.60. 1 hit.
InterProiIPR032023. GCC2_Rab_bind.
IPR000237. GRIP.
[Graphical view]
PfamiPF01465. GRIP. 1 hit.
PF16704. Rab_bind. 1 hit.
[Graphical view]
SMARTiSM00755. Grip. 1 hit.
[Graphical view]
PROSITEiPS50913. GRIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CHG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSAPDAVA AAPSGTPKSK LETLPREDLI KFAKKQMMLL QKAKARCTEL
60 70 80 90 100
DKEVEELKSK PVDGGTDDII KVLTERLDAL LLEKAETEQQ CLCLKKENVK
110 120 130 140 150
MKQEVEDSVT KLEETHKEFE QSHRNYVKEI ESCKNELMAV HSEHSKETAI
160 170 180 190 200
LQKELEEAVH KQVELREQLK SQSDSEDNVR KLQEEIQNIT AAFEEQISCL
210 220 230 240 250
EKKLEATSDE KQQEIIHLQK VIEDKAQHYQ KDINTFQAEI LQLRATHKEE
260 270 280 290 300
VTELMSQIET SAKEHEAEIN KLKENRVTQC EASENIPEKY QCESENLNEV
310 320 330 340 350
ASDASPESQN CSVALQEDPS AEQTVCDKVR QLEDSLKELE SQHSILKDEV
360 370 380 390 400
TYMNNLKLKL EMDAQHIKDE FFHEREDLEF KINELLLAKE EQGYVVEKLK
410 420 430 440 450
YEREDLNRQL CCAVEQHNKE IQRLQEHHQK EVSELSETFI SGSEKEKLAL
460 470 480 490 500
MFEIQGLKEQ CENLQHEKQE VVLNYESLRE MMEILQTELG ESAGKISQEF
510 520 530 540 550
ETMKQQQASD VHELQQKLRS AFNEKDALLE TVNRLQGENE KLLSQQELVP
560 570 580 590 600
ELESTIKNLQ ADNSMYLASL GQKDTMLQEL EAKISSLAKE KDDFISKIKT
610 620 630 640 650
SHEEMDDLHQ KWEREQRLSV ELREAAGQAA QHNSELRQRV SELTGKLDEL
660 670 680 690 700
VREKSQNDQS ITVQMKTMTE DQEALSSKIK SLYEENNRLH SEKAQLSRDL
710 720 730 740 750
EALQAQQDFA HKEHVAEFEK KLQLMVEERD DLNKLLENEQ VQKSFVKTQL
760 770 780 790 800
YEYLKQLRAS ILEENEEEDV VKLIQAVGES LVKVKEEEHN LVFEYDARVL
810 820 830 840 850
ELENKIKCLQ EDSAVQCEEL RTLVRDSEQE KILLRKELDA VTSAKEALQL
860 870 880 890 900
DLLEMKNTNE KASLENQTLS TQVEELSQTL HSRNEVHDEK VLVIEHENLR
910 920 930 940 950
LLLKQRESEL QDVRAELILL KDSLEKSPSV KDQLSLVKEL EEKIESLEKE
960 970 980 990 1000
SKDKDEKISK IKLVAVKAKK ELDSNRKEAQ TLREELESVR SEKDRLSASM
1010 1020 1030 1040 1050
KEFLQGAESY KSLLLEYDKQ SEQLDVEKER AHNFERHIED LTKQLRNSTC
1060 1070 1080 1090 1100
QYERLTSDNE DLLARIETLQ ANAKLLEAQI LEVQKAKGVV EKELDAEELQ
1110 1120 1130 1140 1150
KEQKIKEHVS TVNELEELQL QFQKEKKQLQ KTMQELELVK KDAQQTTLMN
1160 1170 1180 1190 1200
MEIADYERLM KELNQKLTNK NSTIEDLEQE MKIQKEKQET LQEEITSLQS
1210 1220 1230 1240 1250
SVQHYEEKNT KIKQLLVKTK KELADAKQAE TDHLLLQASL KGELEASQQQ
1260 1270 1280 1290 1300
VEVYKIQLAE MTSEKHKIHE HLKTSAEQHQ RTLSAYQQRV VALQEESRAA
1310 1320 1330 1340 1350
KAEQAAVTSE FESYKVRVHN VLKQQKNKSV SQVETEGAKQ EREHLEMLID
1360 1370 1380 1390 1400
QLKIKLQDSQ NSLQISVSEY QTLQAEHDTL LERHNRMLQE TVTKEAELRE
1410 1420 1430 1440 1450
KLCSVQSENT MMKSEHSQTM CQLTSQNEAL RTSFRDQVRH LQDEHRKTVE
1460 1470 1480 1490 1500
TLQHQLSKLE AQLFQLKSEP STRSPASSHQ PSKSLRERRT TDLPLLDMHT
1510 1520 1530 1540 1550
VAREEGEGME TTDSESVSSA GTHIQSLEQL LSSPDTKLER LAETSLWHNE
1560 1570 1580 1590 1600
FTKEELAEKL SSTTKSADHL NGLLRETEAT NAILMEQIKL LKSEIRRLER
1610 1620 1630 1640 1650
NQEREKSVAN LEYLKNVLLR FIFLKPGSER ERLLPVIDTM LQLSPEEKGK
1660 1670
LATVAQGEEE SASRSSGWAS YLHSWSGLR
Length:1,679
Mass (Da):194,445
Last modified:July 3, 2003 - v2
Checksum:i6E2F84263E5E0103
GO

Sequence cautioni

The sequence AAH27339.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti440 – 4401I → M in AAH27339 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027339 mRNA. Translation: AAH27339.1. Different initiation.
AK011206 mRNA. Translation: BAB27466.3.
AK045701 mRNA. Translation: BAC32463.2.
AB093232 Transcribed RNA. Translation: BAC41416.1.
UniGeneiMm.25531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC027339 mRNA. Translation: AAH27339.1. Different initiation.
AK011206 mRNA. Translation: BAB27466.3.
AK045701 mRNA. Translation: BAC32463.2.
AB093232 Transcribed RNA. Translation: BAC41416.1.
UniGeneiMm.25531.

3D structure databases

ProteinModelPortaliQ8CHG3.
SMRiQ8CHG3. Positions 1565-1602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CHG3. 1 interaction.
MINTiMINT-1748522.
STRINGi10090.ENSMUSP00000054033.

PTM databases

iPTMnetiQ8CHG3.
PhosphoSiteiQ8CHG3.

Proteomic databases

EPDiQ8CHG3.
MaxQBiQ8CHG3.
PaxDbiQ8CHG3.
PRIDEiQ8CHG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1917547. Gcc2.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IUCE. Eukaryota.
ENOG410Y42S. LUCA.
HOVERGENiHBG045522.
InParanoidiQ8CHG3.

Miscellaneous databases

PROiQ8CHG3.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GCC2.

Family and domain databases

Gene3Di1.10.220.60. 1 hit.
InterProiIPR032023. GCC2_Rab_bind.
IPR000237. GRIP.
[Graphical view]
PfamiPF01465. GRIP. 1 hit.
PF16704. Rab_bind. 1 hit.
[Graphical view]
SMARTiSM00755. Grip. 1 hit.
[Graphical view]
PROSITEiPS50913. GRIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-769.
    Tissue: Mammary gland.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-443.
    Strain: C57BL/6J.
    Tissue: Brain and Embryo.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 49-1679.
    Tissue: Brain.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 970-977, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiGCC2_MOUSE
AccessioniPrimary (citable) accession number: Q8CHG3
Secondary accession number(s): Q8BR44, Q8R2Q5, Q9CT45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: July 3, 2003
Last modified: March 16, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.