PH domain leucine-rich repeat-containing protein phosphatase 1

Sequence or UniProt identifier

>sp|Q8CHE4|PHLP1_MOUSE PH domain leucine-rich repeat-containing protein phosphatase 1 OS=Mus musculus GN=Phlpp1 PE=2 SV=2 MEPAAAAPAQRLADPTGEDQALAAAAAEGGRCPDPALSAAAPSGGNGGAAREEAPCEAPP GPLPGRAGGTGRRRRRGAPQPAAGGAAPVPAAGGGANSLLLKRGRLKRNLSAAAAASSSS SPSSASSAAGGLPASCSASASLCTRSLDRKTLLLKHRQLLQLQPSDRDWVRHQLQRGCVH VFDRHMASSYLRPVLCTLDTTAAEVAARLLQLGHKGGGVVKVLGYGPPPAAAPAASDQTL DGEHGRDVEPPPSSGTVGAVRGPARAPPADLPLPGGAWTRCAPRISPAPSDSSPGELFAG GPGSPPRAPRPASDTESFSLSPSAESVSDRLDPYSSGGGGSSSSSEELEADPAMPHRPGR PAQPRPPSPKTSALLQPKAPTGVDSTGVIAGEGPGDDKAMAAAAPDVPLSTSGRIRETVQ KTSPPSLYVQLHGETTRRLEADEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFY AGKPHSTGSSERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSVSGKMHV LPLIGGKVEEVKKHQHCLAFSSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDL SCCSLEHLPANLFYSQDLTHLNLKQNFLRQTPTLPAARGLGELQRFTKLKSLNLSNNHLG AFPSAVCSIPTLAELNVSCNALREVPAAVGDMQNLQTFLLDGNFLQSLPAELESMHQLSY LGLSFNEFTDIPEVLEKLTAVDKLCMAGNCVETLRLQALRRMPHIKHVDLRLNILRKLMA DEVDFVQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNVCGYFLKALYASSNEL AQLDVYPVPNYLSYMDVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRK LLAGHNRLARLPERLERTSVEVLDVQHNQITELPPNLLMKADSLRFLNASANKLETLPPA TLSEETSSILQELYLTNNCLTDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEE IDISGNKLKAIPTTIMNCRRMHTVIAHSNCIEVFPEVMQLPEVKCVDLSCNELSEITLPE NLPPKLQELDLTGNPRLALDHKSLELLNNIRCFKIDQPSAGDASGAPAVWSHGYTEASGV KNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQKTKNEEEYMV NTFIVMQRKLGTAGQKLGGAAVLCHIKPDPVDLGGSFTLTSANVGKCQTVLCRNGKPLSL SRSYIMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTP QDEFFILGSKGLWDSLSIDEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSV TEDSFCCCELSAGGSMPPPSPGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTS EMSSEVGSTASDEPPSGVLNESSPAYPNEQRCMLHPVCLSNSFQRQLSSATFSSAFSDNG LDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLLQVPAEASDEGIVISANEDES GLSKKADFSAVGTIGRRRANGSVAPQERSHNVIEVAADAPLRKPGGYFAAPAQPDPDDQF IIPPELEEEVKEIMKHHQEQQQQQQQQQLPPPPQPPQPQPQPQPQPQPQPQRHFQMDHLP DCYDTPL

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Q8CHE4 (PHLP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PH domain leucine-rich repeat-containing protein phosphatase 1
EC=3.1.3.16

Alternative name(s):
Pleckstrin homology domain-containing family E member 1
Short name=PH domain-containing family E member 1
Suprachiasmatic nucleus circadian oscillatory protein

Gene names

Name:	Phlpp1
Synonyms:	Kiaa0606, Phlpp, Plekhe1, Scop

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1687 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB, and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. May act as a negative regulator of K-Ras signaling in membrane rafts By similarity.
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Enzyme regulation	Insensitive to okadaic acid By similarity.
Subunit structure	Interacts with the nucleotide free form of K-Ras (KRAS) via its LRR repeats. Interacts with AKT2, AKT3 and PRKCB By similarity.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Nucleus By similarity.
Induction	Up-regulated in the hippocampus upon chronic methamphetamine treatment. Ref.4
Domain	The PH domain is required for interaction with PRKCB and its dephosphorylation By similarity.
Sequence similarities	Contains 21 LRR (leucine-rich) repeats. Contains 1 PH domain. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAH59254.1 differs from that shown. Reason: Frameshift at position 34.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Membrane Nucleus
Domain	Leucine-rich repeat Repeat
Ligand	Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW entrainment of circadian clock Inferred from mutant phenotype PubMed 20080691. Source: MGI metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1687

1687

PH domain leucine-rich repeat-containing protein phosphatase 1

PRO_0000057782

Regions

Domain

492 – 592

101

Repeat

594 – 615

LRR 1

Repeat

617 – 638

LRR 2

Repeat

648 – 669

LRR 3

Repeat

671 – 692

LRR 4

Repeat

694 – 715

LRR 5

Repeat

717 – 739

LRR 6

Repeat

740 – 760

LRR 7

Repeat

764 – 785

LRR 8

Repeat

788 – 809

LRR 9

Repeat

829 – 850

LRR 10

Repeat

851 – 872

LRR 11

Repeat

874 – 895

LRR 12

Repeat

897 – 918

LRR 13

Repeat

919 – 940

LRR 14

Repeat

943 – 964

LRR 15

Repeat

969 – 989

LRR 16

Repeat

993 – 1014

LRR 17

Repeat

1017 – 1038

LRR 18

Repeat

1040 – 1061

LRR 19

Repeat

1062 – 1083

LRR 20

Repeat

1085 – 1106

LRR 21

Domain

1121 – 1376

256

PP2C-like

Motif

1685 – 1687

PDZ-binding

Compositional bias

4 – 9

Poly-Ala

Compositional bias

21 – 27

Poly-Ala

Compositional bias

112 – 116

Poly-Ala

Compositional bias

337 – 340

Poly-Gly

Compositional bias

341 – 345

Poly-Ser

Compositional bias

399 – 404

Poly-Ala

Compositional bias

1424 – 1427

Poly-Ser

Compositional bias

1638 – 1648

Poly-Gln

Sites

Metal binding

1166

Manganese 1 By similarity

Metal binding

1166

Manganese 2 By similarity

Metal binding

1167

Manganese 1; via carbonyl oxygen By similarity

Metal binding

1330

Manganese 2 By similarity

Metal binding

1369

Manganese 2 By similarity

Experimental info

Sequence conflict

724

S → F in AAH59254. Ref.3

Sequence conflict

1506

E → K in AAH22703. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CHE4 [UniParc].

Last modified June 7, 2005. Version 2.
Checksum: 32B0809AAB3D2412
Show »

FASTA

1,687

182,367

        10         20         30         40         50         60 
MEPAAAAPAQ RLADPTGEDQ ALAAAAAEGG RCPDPALSAA APSGGNGGAA REEAPCEAPP 

        70         80         90        100        110        120 
GPLPGRAGGT GRRRRRGAPQ PAAGGAAPVP AAGGGANSLL LKRGRLKRNL SAAAAASSSS 

       130        140        150        160        170        180 
SPSSASSAAG GLPASCSASA SLCTRSLDRK TLLLKHRQLL QLQPSDRDWV RHQLQRGCVH 

       190        200        210        220        230        240 
VFDRHMASSY LRPVLCTLDT TAAEVAARLL QLGHKGGGVV KVLGYGPPPA AAPAASDQTL 

       250        260        270        280        290        300 
DGEHGRDVEP PPSSGTVGAV RGPARAPPAD LPLPGGAWTR CAPRISPAPS DSSPGELFAG 

       310        320        330        340        350        360 
GPGSPPRAPR PASDTESFSL SPSAESVSDR LDPYSSGGGG SSSSSEELEA DPAMPHRPGR 

       370        380        390        400        410        420 
PAQPRPPSPK TSALLQPKAP TGVDSTGVIA GEGPGDDKAM AAAAPDVPLS TSGRIRETVQ 

       430        440        450        460        470        480 
KTSPPSLYVQ LHGETTRRLE ADEKPLQIQN DYLFQLGFGE LWRVQEEGMD SEIGCLIRFY 

       490        500        510        520        530        540 
AGKPHSTGSS ERIQLSGMYN VRKGKMQLPV NRWTRRQVIL CGTCLIVSSV KDSVSGKMHV 

       550        560        570        580        590        600 
LPLIGGKVEE VKKHQHCLAF SSSGPQSQTY YICFDTFTEY LRWLRQVSKV ASQRISSVDL 

       610        620        630        640        650        660 
SCCSLEHLPA NLFYSQDLTH LNLKQNFLRQ TPTLPAARGL GELQRFTKLK SLNLSNNHLG 

       670        680        690        700        710        720 
AFPSAVCSIP TLAELNVSCN ALREVPAAVG DMQNLQTFLL DGNFLQSLPA ELESMHQLSY 

       730        740        750        760        770        780 
LGLSFNEFTD IPEVLEKLTA VDKLCMAGNC VETLRLQALR RMPHIKHVDL RLNILRKLMA 

       790        800        810        820        830        840 
DEVDFVQHVT QLDLRDNKLG DLDAMIFNNI EVLHCERNQL VTLNVCGYFL KALYASSNEL 

       850        860        870        880        890        900 
AQLDVYPVPN YLSYMDVSRN CLESVPEWVC ESRKLEVLDI GHNQICELPA RLFCNSSLRK 

       910        920        930        940        950        960 
LLAGHNRLAR LPERLERTSV EVLDVQHNQI TELPPNLLMK ADSLRFLNAS ANKLETLPPA 

       970        980        990       1000       1010       1020 
TLSEETSSIL QELYLTNNCL TDKCVPLLTG HPRLKILHMA YNRLQSFPAS KMAKLEELEE 

      1030       1040       1050       1060       1070       1080 
IDISGNKLKA IPTTIMNCRR MHTVIAHSNC IEVFPEVMQL PEVKCVDLSC NELSEITLPE 

      1090       1100       1110       1120       1130       1140 
NLPPKLQELD LTGNPRLALD HKSLELLNNI RCFKIDQPSA GDASGAPAVW SHGYTEASGV 

      1150       1160       1170       1180       1190       1200 
KNKLCVAALS VNNFRDNREA LYGVFDGDRN VEVPYLLQCT MSDILAEELQ KTKNEEEYMV 

      1210       1220       1230       1240       1250       1260 
NTFIVMQRKL GTAGQKLGGA AVLCHIKPDP VDLGGSFTLT SANVGKCQTV LCRNGKPLSL 

      1270       1280       1290       1300       1310       1320 
SRSYIMSCEE ERKRIKQHKA IITEDGKVNG VTESTRILGY TFLHPSVVPR PHVQSVLLTP 

      1330       1340       1350       1360       1370       1380 
QDEFFILGSK GLWDSLSIDE AVEAVRNVPD ALAAAKKLCT LAQSYGCHDS ISAVVVQLSV 

      1390       1400       1410       1420       1430       1440 
TEDSFCCCEL SAGGSMPPPS PGIFPPSVNM VIKDRPSDGL GVPSSSSGMA SEISSELSTS 

      1450       1460       1470       1480       1490       1500 
EMSSEVGSTA SDEPPSGVLN ESSPAYPNEQ RCMLHPVCLS NSFQRQLSSA TFSSAFSDNG 

      1510       1520       1530       1540       1550       1560 
LDSDDEEPIE GVFSNGSRVE VEVDIHCSRA KEKERQQHLL QVPAEASDEG IVISANEDES 

      1570       1580       1590       1600       1610       1620 
GLSKKADFSA VGTIGRRRAN GSVAPQERSH NVIEVAADAP LRKPGGYFAA PAQPDPDDQF 

      1630       1640       1650       1660       1670       1680 
IIPPELEEEV KEIMKHHQEQ QQQQQQQQLP PPPQPPQPQP QPQPQPQPQP QRHFQMDHLP 


DCYDTPL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H. DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 370-1687. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 424-1687. Strain: C57BL/6, Czech II and FVB/N. Tissue: Brain and Mammary tumor.
[4]	"Changes in expression of the mouse homologues of KIAA genes after subchronic methamphetamine treatment." Yamamoto H., Imai K., Takamatsu Y., Kamegaya E., Hara Y., Shimada K., Yamamoto T., Shen H.W., Hagino Y., Kobayashi H., Ide S., Sora I., Koga H., Ikedaa K. Ann. N. Y. Acad. Sci. 1025:92-101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC101663 Genomic DNA. No translation available. AC124710 Genomic DNA. No translation available. AC144773 Genomic DNA. No translation available. AB093251 mRNA. Translation: BAC41435.1. BC004581 mRNA. Translation: AAH04581.1. BC022703 mRNA. Translation: AAH22703.1. BC024670 mRNA. Translation: AAH24670.1. BC059254 mRNA. Translation: AAH59254.1. Frameshift.
IPI	IPI00172310.
RefSeq	NP_598582.3. NM_133821.3.
UniGene	Mm.24115.
3D structure databases
ProteinModelPortal	Q8CHE4.
SMR	Q8CHE4. Positions 584-1107, 1152-1378.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4997715.
PTM databases
PhosphoSite	Q8CHE4.
Proteomic databases
PaxDb	Q8CHE4.
PRIDE	Q8CHE4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000061047; ENSMUSP00000056530; ENSMUSG00000044340.
GeneID	98432.
KEGG	mmu:98432.
UCSC	uc007cgv.2. mouse.
Organism-specific databases
CTD	23239.
MGI	MGI:2138327. Phlpp1.
Rouge	Search...
Phylogenomic databases
eggNOG	COG4886.
GeneTree	ENSGT00440000037833.
HOGENOM	HOG000115529.
InParanoid	Q8CHE4.
KO	K16340.
OMA	CCELSAG.
OrthoDB	EOG4HMJ8G.
Gene expression databases
Bgee	Q8CHE4.
Genevestigator	Q8CHE4.
GermOnline	ENSMUSG00000044340. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit. 3.60.40.10. 1 hit.
InterPro	IPR001611. Leu-rich_rpt. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR001932. PP2C-like. [Graphical view]
Pfam	PF00560. LRR_1. 3 hits. PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS51450. LRR. 17 hits. PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	353478.
SOURCE	Search...

Entry information

Entry name

PHLP1_MOUSE

Accession

Primary (citable) accession number: Q8CHE4
Secondary accession number(s): Q6PCN0

Q99KL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 29, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents