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Protein

Synaptojanin-1

Gene

Synj1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Source: MGI
  • phosphatidylinositol phosphate 5-phosphatase activity Source: MGI
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • inositol phosphate dephosphorylation Source: MGI
  • learning Source: MGI
  • neurotransmitter transport Source: MGI
  • phosphatidylinositol dephosphorylation Source: MGI
  • phosphatidylinositol-mediated signaling Source: MGI
  • phosphatidylinositol metabolic process Source: MGI
  • synaptic vesicle endocytosis Source: MGI
  • synaptic vesicle priming Source: MGI
  • synaptic vesicle transport Source: MGI
  • synaptic vesicle uncoating Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptojanin-1 (EC:3.1.3.36)
Alternative name(s):
Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1
Gene namesi
Name:Synj1
Synonyms:Kiaa0910
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1354961. Synj1.

Subcellular locationi

GO - Cellular componenti

  • clathrin coat Source: MGI
  • membrane coat Source: MGI
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15741574Synaptojanin-1PRO_0000209731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei820 – 8201PhosphoserineCombined sources
Modified residuei830 – 8301PhosphoserineCombined sources
Modified residuei1053 – 10531PhosphoserineBy similarity
Modified residuei1147 – 11471PhosphoserineCombined sources
Modified residuei1175 – 11751PhosphoserineBy similarity
Modified residuei1217 – 12171PhosphothreonineBy similarity
Modified residuei1350 – 13501PhosphoserineCombined sources
Modified residuei1354 – 13541PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CHC4.
MaxQBiQ8CHC4.
PaxDbiQ8CHC4.
PRIDEiQ8CHC4.

PTM databases

iPTMnetiQ8CHC4.
PhosphoSiteiQ8CHC4.
SwissPalmiQ8CHC4.

Expressioni

Gene expression databases

CleanExiMM_SYNJ1.

Interactioni

Subunit structurei

Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with ASH/GRB2. Interacts with MYO1E (via SH3 domain) (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3.By similarity1 Publication

Protein-protein interaction databases

IntActiQ8CHC4. 4 interactions.
MINTiMINT-268854.
STRINGi10090.ENSMUSP00000113308.

Structurei

3D structure databases

ProteinModelPortaliQ8CHC4.
SMRiQ8CHC4. Positions 895-971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 442324SACPROSITE-ProRule annotationAdd
BLAST
Domaini894 – 97178RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1042 – 1286245Pro-richAdd
BLAST
Compositional biasi1533 – 156634Pro-richAdd
BLAST

Domaini

Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.By similarity
The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.

Sequence similaritiesi

Belongs to the synaptojanin family.Curated
In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation
Contains 1 SAC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0566. Eukaryota.
COG5329. LUCA.
COG5411. LUCA.
HOGENOMiHOG000007937.
HOVERGENiHBG079225.
InParanoidiQ8CHC4.
PhylomeDBiQ8CHC4.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
3.60.10.10. 1 hit.
InterProiIPR015047. DUF1866.
IPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF08952. DUF1866. 1 hit.
PF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM01165. DUF1866. 1 hit.
SM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF56219. SSF56219. 2 hits.
PROSITEiPS50102. RRM. 1 hit.
PS50275. SAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CHC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSKGFRIY HKLDPPPFSL IVETRHKEEC LMFESGAVAV LSSAEKEAIK
60 70 80 90 100
GTYAKVLDAY GLLGVLRLNL GDTMLHYLVL VTGCMSVGKI QESEVFRVTS
110 120 130 140 150
TEFISLRVDA SDEDRISEVR KVLNSGNFYF AWSASGVSLD LSLNAHRSMQ
160 170 180 190 200
EHTTDNRFFW NQSLHLHLKH YGVNCDDWLL RLMCGGVEIR TIYAAHKQAK
210 220 230 240 250
ACLISRLSCE RAGTRFNVRG TNDDGHVANF VETEQVIYLD DCVSSFIQIR
260 270 280 290 300
GSVPLFWEQP GLQVGSHRVR MSRGFEANAP AFDRHFRTLK DLYGKQIVVN
310 320 330 340 350
LLGSKEGEHM LSKAFQSHLK ASEHASDIHM VSFDYHQMVK GGKAEKLHSI
360 370 380 390 400
LKPQVQKFLD YGFFYFDGSE VQRCQSGTVR TNCLDCLDRT NSVQAFLGLE
410 420 430 440 450
MLAKQLEALG LAEKPQLVTR FQEVFRSMWS VNGDSISKIY AGTGALEGKA
460 470 480 490 500
KLKDGARSVT RTIQNNFFDS SKQEAIDVLL LGNTLNSDLA DKARALLTTG
510 520 530 540 550
SLRVSEQTLQ SASSKVLKNM CENFYKYSKP KKIRVCVGTW NVNGGKQFRS
560 570 580 590 600
IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK PTDIFAIGFE EMVELNAGNI
610 620 630 640 650
VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV FIRPQHAPFI
660 670 680 690 700
RDVAVDTVKT GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER
710 720 730 740 750
NEDFVEIARK LSFPMGRMLF SHDYVFWCGD FNYRIDLPNE EVKELIRQQN
760 770 780 790 800
WDSLIAGDQL INQKNAGQIF RGFLEGKVTF APTYKYDLFS EDYDTSEKCR
810 820 830 840 850
TPAWTDRVLW RRRKWPFDRS AEDLDLLNAS FQDESKILYT WTPGTLLHYG
860 870 880 890 900
RAELKTSDHR PVVALIDIDI FEVEAEERQK IYKEVIAVQG PPDGTVLVSI
910 920 930 940 950
KSSAQESTFF DDALIDELLR QFAHFGEVIL IRFVEDKMWV TFLEGSSALN
960 970 980 990 1000
ALSLNGKELL NRTITITLKS PDWIKHLEEE MSLEKISVTL PSSASSTLLG
1010 1020 1030 1040 1050
EDAEVAADFD MEGDVDDYSA EVEELLPQHL QPSSSSGLGT SPSSSPRTSP
1060 1070 1080 1090 1100
CQSPTVPEYS APSLPIRPSR APSRTPGPPS SQGSPVDTQP AAQKDSSQTL
1110 1120 1130 1140 1150
EPKRPPPPRP VAPPARPAPP QRPPPPSGAR SPAPARKEFG GVGAPPSPGV
1160 1170 1180 1190 1200
ARREIEAPKS PGTARKDNIG RNQPSPQAGL AGPGPAGYGA ARPTIPARAG
1210 1220 1230 1240 1250
VISAPQSQAR VCAGRPTPDS QSKPSETLKG PAVLPEPLKP QAAFPQQPSL
1260 1270 1280 1290 1300
PTPAQKLQDP LVPIAAPTMP PSGPQPNLET PPQPPPRSRS SQSLPSDSSP
1310 1320 1330 1340 1350
QLQQEQPTGQ VKINGISGVK QEPTLKSDPF EDLSLSVLAV SKAQPSVQIS
1360 1370 1380 1390 1400
PVLTPDPKML IQLPSASQSQ VNPLSSVSCM PTRPPGPEES KSQESMGSSA
1410 1420 1430 1440 1450
NPFPSLPCRN PFTDRTAAPG NPFRVQSQES EATSWLSKEE PVPNSPFPPL
1460 1470 1480 1490 1500
MPLSHDTSKA SSSLGGFEDN FDLQSQSTVK TSNPKGWVTF DEDDNFPTTG
1510 1520 1530 1540 1550
KSKSVCPDLV GNAPASFDDD WSKGASVSFC VLPARRPPPP PPPVPLLPPG
1560 1570
TTSSAGPSTT LPSKAPSTLD FTER
Length:1,574
Mass (Da):172,617
Last modified:January 16, 2004 - v3
Checksum:iF791EE3AD6A37B82
GO

Sequence cautioni

The sequence BAC41456.2 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093272 Transcribed RNA. Translation: BAC41456.2. Different initiation.
UniGeneiMm.187079.

Genome annotation databases

UCSCiuc007zww.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093272 Transcribed RNA. Translation: BAC41456.2. Different initiation.
UniGeneiMm.187079.

3D structure databases

ProteinModelPortaliQ8CHC4.
SMRiQ8CHC4. Positions 895-971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CHC4. 4 interactions.
MINTiMINT-268854.
STRINGi10090.ENSMUSP00000113308.

PTM databases

iPTMnetiQ8CHC4.
PhosphoSiteiQ8CHC4.
SwissPalmiQ8CHC4.

Proteomic databases

EPDiQ8CHC4.
MaxQBiQ8CHC4.
PaxDbiQ8CHC4.
PRIDEiQ8CHC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007zww.2. mouse.

Organism-specific databases

MGIiMGI:1354961. Synj1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0566. Eukaryota.
COG5329. LUCA.
COG5411. LUCA.
HOGENOMiHOG000007937.
HOVERGENiHBG079225.
InParanoidiQ8CHC4.
PhylomeDBiQ8CHC4.

Miscellaneous databases

ChiTaRSiSynj1. mouse.
PROiQ8CHC4.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SYNJ1.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
3.60.10.10. 1 hit.
InterProiIPR015047. DUF1866.
IPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF08952. DUF1866. 1 hit.
PF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM01165. DUF1866. 1 hit.
SM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
SSF56219. SSF56219. 2 hits.
PROSITEiPS50102. RRM. 1 hit.
PS50275. SAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
    DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis."
    Modregger J., Ritter B., Witter B., Paulsson M., Plomann M.
    J. Cell Sci. 113:4511-4521(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PACSIN1; PACSIN2 AND PACSIN3.
  4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-820; SER-830; SER-1147; SER-1350 AND THR-1354, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiSYNJ1_MOUSE
AccessioniPrimary (citable) accession number: Q8CHC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: May 11, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.