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Protein

Synaptojanin-1

Gene

Synj1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphatase that acts on various phosphoinositides, including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate (By similarity). Has a role in clathrin-mediated endocytosis (By similarity). Hydrolyzes PIP2 bound to actin regulatory proteins resulting in the rearrangement of actin filaments downstream of tyrosine kinase and ASH/GRB2 (By similarity).By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

GO - Molecular functioni

  • phosphatidylinositol-3-phosphatase activity Source: MGI
  • phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Source: MGI
  • phosphatidylinositol-4-phosphate phosphatase activity Source: MGI
  • phosphatidylinositol phosphate 5-phosphatase activity Source: MGI
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • inositol phosphate dephosphorylation Source: MGI
  • learning Source: MGI
  • neurotransmitter transport Source: MGI
  • phosphatidylinositol dephosphorylation Source: MGI
  • phosphatidylinositol-mediated signaling Source: MGI
  • phosphatidylinositol metabolic process Source: MGI
  • positive regulation of endosome organization Source: MGI
  • positive regulation of synaptic vesicle recycling Source: BHF-UCL
  • postsynaptic neurotransmitter receptor internalization Source: SynGO
  • synaptic vesicle endocytosis Source: MGI
  • synaptic vesicle priming Source: MGI
  • synaptic vesicle transport Source: MGI
  • synaptic vesicle uncoating Source: SynGO

Keywordsi

Molecular functionHydrolase, RNA-binding
Biological processEndocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptojanin-1 (EC:3.1.3.36)
Alternative name(s):
Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1
Gene namesi
Name:Synj1
Synonyms:Kiaa0910
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1354961 Synj1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002097311 – 1574Synaptojanin-1Add BLAST1574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei820PhosphoserineCombined sources1
Modified residuei830PhosphoserineCombined sources1
Modified residuei1053PhosphoserineBy similarity1
Modified residuei1147PhosphoserineCombined sources1
Modified residuei1175PhosphoserineBy similarity1
Modified residuei1198Omega-N-methylarginineCombined sources1
Modified residuei1217PhosphothreonineBy similarity1
Modified residuei1290PhosphoserineBy similarity1
Modified residuei1350PhosphoserineCombined sources1
Modified residuei1354PhosphothreonineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ8CHC4
MaxQBiQ8CHC4
PaxDbiQ8CHC4
PeptideAtlasiQ8CHC4
PRIDEiQ8CHC4

PTM databases

iPTMnetiQ8CHC4
PhosphoSitePlusiQ8CHC4
SwissPalmiQ8CHC4

Expressioni

Gene expression databases

CleanExiMM_SYNJ1

Interactioni

Subunit structurei

Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with ASH/GRB2. Interacts with MYO1E (via SH3 domain) (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3.By similarity1 Publication

Protein-protein interaction databases

IntActiQ8CHC4, 6 interactors
MINTiQ8CHC4
STRINGi10090.ENSMUSP00000113308

Structurei

3D structure databases

ProteinModelPortaliQ8CHC4
SMRiQ8CHC4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini119 – 442SACPROSITE-ProRule annotationAdd BLAST324
Domaini894 – 971RRMPROSITE-ProRule annotationAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1042 – 1286Pro-richAdd BLAST245
Compositional biasi1533 – 1566Pro-richAdd BLAST34

Domaini

Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.By similarity
The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.

Sequence similaritiesi

Belongs to the synaptojanin family.Curated
In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0566 Eukaryota
COG5329 LUCA
COG5411 LUCA
HOGENOMiHOG000007937
HOVERGENiHBG079225
InParanoidiQ8CHC4
PhylomeDBiQ8CHC4

Family and domain databases

CDDicd12719 RRM_SYNJ1, 1 hit
Gene3Di3.30.70.330, 1 hit
3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR015047 DUF1866
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR000300 IPPc
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR002013 SAC_dom
IPR034972 SYNJ1
IPR034971 SYNJ1_RRM
PANTHERiPTHR11200:SF158 PTHR11200:SF158, 2 hits
PfamiView protein in Pfam
PF08952 DUF1866, 1 hit
PF03372 Exo_endo_phos, 1 hit
PF02383 Syja_N, 1 hit
SMARTiView protein in SMART
SM01165 DUF1866, 1 hit
SM00128 IPPc, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF56219 SSF56219, 2 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50275 SAC, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CHC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSKGFRIY HKLDPPPFSL IVETRHKEEC LMFESGAVAV LSSAEKEAIK
60 70 80 90 100
GTYAKVLDAY GLLGVLRLNL GDTMLHYLVL VTGCMSVGKI QESEVFRVTS
110 120 130 140 150
TEFISLRVDA SDEDRISEVR KVLNSGNFYF AWSASGVSLD LSLNAHRSMQ
160 170 180 190 200
EHTTDNRFFW NQSLHLHLKH YGVNCDDWLL RLMCGGVEIR TIYAAHKQAK
210 220 230 240 250
ACLISRLSCE RAGTRFNVRG TNDDGHVANF VETEQVIYLD DCVSSFIQIR
260 270 280 290 300
GSVPLFWEQP GLQVGSHRVR MSRGFEANAP AFDRHFRTLK DLYGKQIVVN
310 320 330 340 350
LLGSKEGEHM LSKAFQSHLK ASEHASDIHM VSFDYHQMVK GGKAEKLHSI
360 370 380 390 400
LKPQVQKFLD YGFFYFDGSE VQRCQSGTVR TNCLDCLDRT NSVQAFLGLE
410 420 430 440 450
MLAKQLEALG LAEKPQLVTR FQEVFRSMWS VNGDSISKIY AGTGALEGKA
460 470 480 490 500
KLKDGARSVT RTIQNNFFDS SKQEAIDVLL LGNTLNSDLA DKARALLTTG
510 520 530 540 550
SLRVSEQTLQ SASSKVLKNM CENFYKYSKP KKIRVCVGTW NVNGGKQFRS
560 570 580 590 600
IAFKNQTLTD WLLDAPKLAG IQEFQDKRSK PTDIFAIGFE EMVELNAGNI
610 620 630 640 650
VNASTTNQKL WAVELQKTIS RDNKYVLLAS EQLVGVCLFV FIRPQHAPFI
660 670 680 690 700
RDVAVDTVKT GMGGATGNKG AVAIRMLFHT TSLCFVCSHF AAGQSQVKER
710 720 730 740 750
NEDFVEIARK LSFPMGRMLF SHDYVFWCGD FNYRIDLPNE EVKELIRQQN
760 770 780 790 800
WDSLIAGDQL INQKNAGQIF RGFLEGKVTF APTYKYDLFS EDYDTSEKCR
810 820 830 840 850
TPAWTDRVLW RRRKWPFDRS AEDLDLLNAS FQDESKILYT WTPGTLLHYG
860 870 880 890 900
RAELKTSDHR PVVALIDIDI FEVEAEERQK IYKEVIAVQG PPDGTVLVSI
910 920 930 940 950
KSSAQESTFF DDALIDELLR QFAHFGEVIL IRFVEDKMWV TFLEGSSALN
960 970 980 990 1000
ALSLNGKELL NRTITITLKS PDWIKHLEEE MSLEKISVTL PSSASSTLLG
1010 1020 1030 1040 1050
EDAEVAADFD MEGDVDDYSA EVEELLPQHL QPSSSSGLGT SPSSSPRTSP
1060 1070 1080 1090 1100
CQSPTVPEYS APSLPIRPSR APSRTPGPPS SQGSPVDTQP AAQKDSSQTL
1110 1120 1130 1140 1150
EPKRPPPPRP VAPPARPAPP QRPPPPSGAR SPAPARKEFG GVGAPPSPGV
1160 1170 1180 1190 1200
ARREIEAPKS PGTARKDNIG RNQPSPQAGL AGPGPAGYGA ARPTIPARAG
1210 1220 1230 1240 1250
VISAPQSQAR VCAGRPTPDS QSKPSETLKG PAVLPEPLKP QAAFPQQPSL
1260 1270 1280 1290 1300
PTPAQKLQDP LVPIAAPTMP PSGPQPNLET PPQPPPRSRS SQSLPSDSSP
1310 1320 1330 1340 1350
QLQQEQPTGQ VKINGISGVK QEPTLKSDPF EDLSLSVLAV SKAQPSVQIS
1360 1370 1380 1390 1400
PVLTPDPKML IQLPSASQSQ VNPLSSVSCM PTRPPGPEES KSQESMGSSA
1410 1420 1430 1440 1450
NPFPSLPCRN PFTDRTAAPG NPFRVQSQES EATSWLSKEE PVPNSPFPPL
1460 1470 1480 1490 1500
MPLSHDTSKA SSSLGGFEDN FDLQSQSTVK TSNPKGWVTF DEDDNFPTTG
1510 1520 1530 1540 1550
KSKSVCPDLV GNAPASFDDD WSKGASVSFC VLPARRPPPP PPPVPLLPPG
1560 1570
TTSSAGPSTT LPSKAPSTLD FTER
Length:1,574
Mass (Da):172,617
Last modified:January 16, 2004 - v3
Checksum:iF791EE3AD6A37B82
GO

Sequence cautioni

The sequence BAC41456 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093272 Transcribed RNA Translation: BAC41456.2 Different initiation.
UniGeneiMm.187079

Genome annotation databases

UCSCiuc007zww.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSYNJ1_MOUSE
AccessioniPrimary (citable) accession number: Q8CHC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: May 23, 2018
This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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