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Q8CH87 (GCNT3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

EC=2.4.1.102
EC=2.4.1.150
Alternative name(s):
C2GnT-mucin type
Short name=C2GnT-M
dI/C2/C4GnT
Short name=dIGnT
Gene names
Name:Gcnt3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the glycosyltransferase 14 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7-8.5. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 437437Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
PRO_0000288547

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2721Helical; Signal-anchor for type II membrane protein; Potential
Topological domain28 – 437410Lumenal Potential

Amino acid modifications

Glycosylation2881N-linked (GlcNAc...) Potential
Disulfide bond70 ↔ 227 By similarity
Disulfide bond161 ↔ 381 By similarity
Disulfide bond182 ↔ 209 By similarity
Disulfide bond390 ↔ 422 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CH87 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 94CA5C1B883A1D82

FASTA43750,642
        10         20         30         40         50         60 
MVSWRRFCWH YHGWTLGCYM LLAIIALKLS LRLKCDFDVM DLDSKEFQSQ YCRDLLYKTL 

        70         80         90        100        110        120 
ELPAKSSINC SGVIRGEQKA VTQALLNNLE LKRKRQSFTE ADYLSMTADC EHFKTQRKFI 

       130        140        150        160        170        180 
QVPLSKEEAN FPIAYSMVIH EKIENFERLL RAVYTPQNIY CVHVDQKSSE TFQQAVRAIV 

       190        200        210        220        230        240 
SCFPNVFIAN KLVSVVYASW SRVQADLNCM EDLLQSPVPW EYLLNTCGTD FPIKTNAEMV 

       250        260        270        280        290        300 
KALKLLNGQN SMESEVPPPH KTFRWKYHYE VADTLYRTSK EKTPPPNNIT MFTGNAYMVA 

       310        320        330        340        350        360 
SRDFIEHVLS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHPK FDLSDMRSIA 

       370        380        390        400        410        420 
RLTKWQDHEG DIENGAPYTS CSGIHQRAIC VYGSGDLHWI LQNHHLLANK FDPKVDDNVL 

       430 
QCLEEYLRHK AIYGTEL 

« Hide

References

[1]"Purification and cDNA cloning of UDP-GlcNAc:GlcNAcbeta1-3Galbeta1-4Glc(NAc)-R [GlcNAc to Gal]beta1,6N-acetylglucosaminyltransferase from rat small intestine: a major carrier of dIGnT activity in rat small intestine."
Korekane H., Taguchi T., Sakamoto Y., Honke K., Dohmae N., Salminen H., Toivonen S., Helin J., Takio K., Renkonen O., Taniguchi N.
Glycobiology 13:387-400(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 118-126; 266-280 AND 313-324, BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB098520 mRNA. Translation: BAC53607.1.
RefSeqNP_775434.1. NM_173312.2.
XP_006243426.1. XM_006243364.1.
XP_006243427.1. XM_006243365.1.
XP_006243428.1. XM_006243366.1.
XP_006243429.1. XM_006243367.1.
XP_006243430.1. XM_006243368.1.
UniGeneRn.43518.

3D structure databases

ProteinModelPortalQ8CH87.
SMRQ8CH87. Positions 67-432.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000014727.

Protein family/group databases

CAZyGT14. Glycosyltransferase Family 14.

PTM databases

PhosphoSiteQ8CH87.

Proteomic databases

PRIDEQ8CH87.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000014727; ENSRNOP00000014727; ENSRNOG00000011086.
GeneID286976.
KEGGrno:286976.
UCSCRGD:631333. rat.

Organism-specific databases

CTD9245.
RGD631333. Gcnt3.

Phylogenomic databases

eggNOGNOG253565.
GeneTreeENSGT00750000117608.
HOGENOMHOG000293251.
HOVERGENHBG051711.
InParanoidQ8CH87.
KOK09662.
OMAMTADCEH.
OrthoDBEOG74N5HW.
PhylomeDBQ8CH87.
TreeFamTF315534.

Enzyme and pathway databases

BRENDA2.4.1.164. 5301.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ8CH87.

Family and domain databases

InterProIPR003406. Glyco_trans_14.
[Graphical view]
PfamPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio625213.
PROQ8CH87.

Entry information

Entry nameGCNT3_RAT
AccessionPrimary (citable) accession number: Q8CH87
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways