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Q8CH87

- GCNT3_RAT

UniProt

Q8CH87 - GCNT3_RAT

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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

Gcnt3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

pH dependencei

Optimum pH is 7-8.5.1 Publication

Pathwayi

GO - Molecular functioni

  1. acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: Ensembl
  2. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  3. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: RGD

GO - Biological processi

  1. immunoglobulin production in mucosal tissue Source: Ensembl
  2. intestinal absorption Source: Ensembl
  3. kidney morphogenesis Source: Ensembl
  4. protein glycosylation Source: UniProtKB-UniPathway
  5. tissue morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.164. 5301.
ReactomeiREACT_196259. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
dI/C2/C4GnT
Short name:
dIGnT
Gene namesi
Name:Gcnt3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi631333. Gcnt3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini28 – 437410LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288547Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 227By similarity
Disulfide bondi161 ↔ 381By similarity
Disulfide bondi182 ↔ 209By similarity
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi390 ↔ 422By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8CH87.

PTM databases

PhosphoSiteiQ8CH87.

Expressioni

Gene expression databases

GenevestigatoriQ8CH87.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014727.

Structurei

3D structure databases

ProteinModelPortaliQ8CH87.
SMRiQ8CH87. Positions 67-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253565.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ8CH87.
KOiK09662.
OMAiMTADCEH.
OrthoDBiEOG74N5HW.
PhylomeDBiQ8CH87.
TreeFamiTF315534.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CH87-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVSWRRFCWH YHGWTLGCYM LLAIIALKLS LRLKCDFDVM DLDSKEFQSQ
60 70 80 90 100
YCRDLLYKTL ELPAKSSINC SGVIRGEQKA VTQALLNNLE LKRKRQSFTE
110 120 130 140 150
ADYLSMTADC EHFKTQRKFI QVPLSKEEAN FPIAYSMVIH EKIENFERLL
160 170 180 190 200
RAVYTPQNIY CVHVDQKSSE TFQQAVRAIV SCFPNVFIAN KLVSVVYASW
210 220 230 240 250
SRVQADLNCM EDLLQSPVPW EYLLNTCGTD FPIKTNAEMV KALKLLNGQN
260 270 280 290 300
SMESEVPPPH KTFRWKYHYE VADTLYRTSK EKTPPPNNIT MFTGNAYMVA
310 320 330 340 350
SRDFIEHVLS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHPK
360 370 380 390 400
FDLSDMRSIA RLTKWQDHEG DIENGAPYTS CSGIHQRAIC VYGSGDLHWI
410 420 430
LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL
Length:437
Mass (Da):50,642
Last modified:March 1, 2003 - v1
Checksum:i94CA5C1B883A1D82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB098520 mRNA. Translation: BAC53607.1.
RefSeqiNP_775434.1. NM_173312.2.
XP_006243428.1. XM_006243366.2.
XP_008764442.1. XM_008766220.1.
UniGeneiRn.43518.

Genome annotation databases

EnsembliENSRNOT00000014727; ENSRNOP00000014727; ENSRNOG00000011086.
GeneIDi286976.
KEGGirno:286976.
UCSCiRGD:631333. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB098520 mRNA. Translation: BAC53607.1 .
RefSeqi NP_775434.1. NM_173312.2.
XP_006243428.1. XM_006243366.2.
XP_008764442.1. XM_008766220.1.
UniGenei Rn.43518.

3D structure databases

ProteinModelPortali Q8CH87.
SMRi Q8CH87. Positions 67-432.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000014727.

Protein family/group databases

CAZyi GT14. Glycosyltransferase Family 14.

PTM databases

PhosphoSitei Q8CH87.

Proteomic databases

PRIDEi Q8CH87.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000014727 ; ENSRNOP00000014727 ; ENSRNOG00000011086 .
GeneIDi 286976.
KEGGi rno:286976.
UCSCi RGD:631333. rat.

Organism-specific databases

CTDi 9245.
RGDi 631333. Gcnt3.

Phylogenomic databases

eggNOGi NOG253565.
GeneTreei ENSGT00760000119183.
HOGENOMi HOG000293251.
HOVERGENi HBG051711.
InParanoidi Q8CH87.
KOi K09662.
OMAi MTADCEH.
OrthoDBi EOG74N5HW.
PhylomeDBi Q8CH87.
TreeFami TF315534.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.164. 5301.
Reactomei REACT_196259. O-linked glycosylation of mucins.

Miscellaneous databases

NextBioi 625213.
PROi Q8CH87.

Gene expression databases

Genevestigatori Q8CH87.

Family and domain databases

InterProi IPR003406. Glyco_trans_14.
[Graphical view ]
Pfami PF02485. Branch. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification and cDNA cloning of UDP-GlcNAc:GlcNAcbeta1-3Galbeta1-4Glc(NAc)-R [GlcNAc to Gal]beta1,6N-acetylglucosaminyltransferase from rat small intestine: a major carrier of dIGnT activity in rat small intestine."
    Korekane H., Taguchi T., Sakamoto Y., Honke K., Dohmae N., Salminen H., Toivonen S., Helin J., Takio K., Renkonen O., Taniguchi N.
    Glycobiology 13:387-400(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 118-126; 266-280 AND 313-324, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiGCNT3_RAT
AccessioniPrimary (citable) accession number: Q8CH87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3