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Q8CH87

- GCNT3_RAT

UniProt

Q8CH87 - GCNT3_RAT

Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

Gcnt3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

    pH dependencei

    Optimum pH is 7-8.5.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: Ensembl
    2. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    3. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: RGD

    GO - Biological processi

    1. immunoglobulin production in mucosal tissue Source: Ensembl
    2. intestinal absorption Source: Ensembl
    3. kidney morphogenesis Source: Ensembl
    4. protein glycosylation Source: UniProtKB-UniPathway
    5. tissue morphogenesis Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.164. 5301.
    ReactomeiREACT_196259. O-linked glycosylation of mucins.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT14. Glycosyltransferase Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
    Alternative name(s):
    C2GnT-mucin type
    Short name:
    C2GnT-M
    dI/C2/C4GnT
    Short name:
    dIGnT
    Gene namesi
    Name:Gcnt3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi631333. Gcnt3.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 437437Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288547Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi70 ↔ 227By similarity
    Disulfide bondi161 ↔ 381By similarity
    Disulfide bondi182 ↔ 209By similarity
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi390 ↔ 422By similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8CH87.

    PTM databases

    PhosphoSiteiQ8CH87.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8CH87.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000014727.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CH87.
    SMRiQ8CH87. Positions 67-432.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini28 – 437410LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 14 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG253565.
    GeneTreeiENSGT00750000117608.
    HOGENOMiHOG000293251.
    HOVERGENiHBG051711.
    InParanoidiQ8CH87.
    KOiK09662.
    OMAiMTADCEH.
    OrthoDBiEOG74N5HW.
    PhylomeDBiQ8CH87.
    TreeFamiTF315534.

    Family and domain databases

    InterProiIPR003406. Glyco_trans_14.
    [Graphical view]
    PfamiPF02485. Branch. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CH87-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSWRRFCWH YHGWTLGCYM LLAIIALKLS LRLKCDFDVM DLDSKEFQSQ    50
    YCRDLLYKTL ELPAKSSINC SGVIRGEQKA VTQALLNNLE LKRKRQSFTE 100
    ADYLSMTADC EHFKTQRKFI QVPLSKEEAN FPIAYSMVIH EKIENFERLL 150
    RAVYTPQNIY CVHVDQKSSE TFQQAVRAIV SCFPNVFIAN KLVSVVYASW 200
    SRVQADLNCM EDLLQSPVPW EYLLNTCGTD FPIKTNAEMV KALKLLNGQN 250
    SMESEVPPPH KTFRWKYHYE VADTLYRTSK EKTPPPNNIT MFTGNAYMVA 300
    SRDFIEHVLS NSKARQLIEW VKDTYSPDEH LWATLQRASW MPGSDPLHPK 350
    FDLSDMRSIA RLTKWQDHEG DIENGAPYTS CSGIHQRAIC VYGSGDLHWI 400
    LQNHHLLANK FDPKVDDNVL QCLEEYLRHK AIYGTEL 437
    Length:437
    Mass (Da):50,642
    Last modified:March 1, 2003 - v1
    Checksum:i94CA5C1B883A1D82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB098520 mRNA. Translation: BAC53607.1.
    RefSeqiNP_775434.1. NM_173312.2.
    XP_006243426.1. XM_006243364.1.
    XP_006243427.1. XM_006243365.1.
    XP_006243428.1. XM_006243366.1.
    XP_006243429.1. XM_006243367.1.
    XP_006243430.1. XM_006243368.1.
    UniGeneiRn.43518.

    Genome annotation databases

    EnsembliENSRNOT00000014727; ENSRNOP00000014727; ENSRNOG00000011086.
    GeneIDi286976.
    KEGGirno:286976.
    UCSCiRGD:631333. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB098520 mRNA. Translation: BAC53607.1 .
    RefSeqi NP_775434.1. NM_173312.2.
    XP_006243426.1. XM_006243364.1.
    XP_006243427.1. XM_006243365.1.
    XP_006243428.1. XM_006243366.1.
    XP_006243429.1. XM_006243367.1.
    XP_006243430.1. XM_006243368.1.
    UniGenei Rn.43518.

    3D structure databases

    ProteinModelPortali Q8CH87.
    SMRi Q8CH87. Positions 67-432.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000014727.

    Protein family/group databases

    CAZyi GT14. Glycosyltransferase Family 14.

    PTM databases

    PhosphoSitei Q8CH87.

    Proteomic databases

    PRIDEi Q8CH87.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000014727 ; ENSRNOP00000014727 ; ENSRNOG00000011086 .
    GeneIDi 286976.
    KEGGi rno:286976.
    UCSCi RGD:631333. rat.

    Organism-specific databases

    CTDi 9245.
    RGDi 631333. Gcnt3.

    Phylogenomic databases

    eggNOGi NOG253565.
    GeneTreei ENSGT00750000117608.
    HOGENOMi HOG000293251.
    HOVERGENi HBG051711.
    InParanoidi Q8CH87.
    KOi K09662.
    OMAi MTADCEH.
    OrthoDBi EOG74N5HW.
    PhylomeDBi Q8CH87.
    TreeFami TF315534.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 2.4.1.164. 5301.
    Reactomei REACT_196259. O-linked glycosylation of mucins.

    Miscellaneous databases

    NextBioi 625213.
    PROi Q8CH87.

    Gene expression databases

    Genevestigatori Q8CH87.

    Family and domain databases

    InterProi IPR003406. Glyco_trans_14.
    [Graphical view ]
    Pfami PF02485. Branch. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification and cDNA cloning of UDP-GlcNAc:GlcNAcbeta1-3Galbeta1-4Glc(NAc)-R [GlcNAc to Gal]beta1,6N-acetylglucosaminyltransferase from rat small intestine: a major carrier of dIGnT activity in rat small intestine."
      Korekane H., Taguchi T., Sakamoto Y., Honke K., Dohmae N., Salminen H., Toivonen S., Helin J., Takio K., Renkonen O., Taniguchi N.
      Glycobiology 13:387-400(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 118-126; 266-280 AND 313-324, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiGCNT3_RAT
    AccessioniPrimary (citable) accession number: Q8CH87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3