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Protein

E3 ubiquitin-protein ligase TRIM32

Gene

Trim32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin). May ubiquitinate BBS2 (By similarity). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha.By similarity2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri21 – 6646RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri96 – 13944B box-type; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • myosin binding Source: MGI
  • protein self-association Source: BHF-UCL
  • RNA binding Source: BHF-UCL
  • translation initiation factor binding Source: MGI
  • ubiquitin binding Source: BHF-UCL
  • ubiquitin protein ligase activity Source: BHF-UCL
  • ubiquitin-protein transferase activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • actin ubiquitination Source: MGI
  • axon development Source: MGI
  • fat cell differentiation Source: BHF-UCL
  • innate immune response Source: MGI
  • muscle cell cellular homeostasis Source: MGI
  • negative regulation of fibroblast proliferation Source: BHF-UCL
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage Source: BHF-UCL
  • negative regulation of keratinocyte apoptotic process Source: BHF-UCL
  • negative regulation of viral release from host cell Source: MGI
  • negative regulation of viral transcription Source: MGI
  • positive regulation of cell cycle Source: BHF-UCL
  • positive regulation of cell growth Source: BHF-UCL
  • positive regulation of cell migration Source: BHF-UCL
  • positive regulation of cell motility Source: BHF-UCL
  • positive regulation of chemokine (C-C motif) ligand 20 production Source: MGI
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • positive regulation of interleukin-17-mediated signaling pathway Source: MGI
  • positive regulation of neurogenesis Source: BHF-UCL
  • positive regulation of neuron differentiation Source: BHF-UCL
  • positive regulation of NF-kappaB transcription factor activity Source: BHF-UCL
  • positive regulation of protein catabolic process Source: BHF-UCL
  • positive regulation of proteolysis Source: BHF-UCL
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • positive regulation of striated muscle cell differentiation Source: MGI
  • positive regulation of tumor necrosis factor-mediated signaling pathway Source: MGI
  • protein polyubiquitination Source: BHF-UCL
  • protein ubiquitination Source: BHF-UCL
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: BHF-UCL
  • response to tumor necrosis factor Source: BHF-UCL
  • response to UV Source: BHF-UCL
  • tissue homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3134975. Regulation of innate immune responses to cytosolic DNA.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM32 (EC:6.3.2.-)
Alternative name(s):
Tripartite motif-containing protein 32
Gene namesi
Name:Trim32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917057. Trim32.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655E3 ubiquitin-protein ligase TRIM32PRO_0000056247Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei330 – 3301PhosphoserineCombined sources
Modified residuei337 – 3371PhosphoserineCombined sources
Modified residuei341 – 3411PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CH72.
MaxQBiQ8CH72.
PaxDbiQ8CH72.
PRIDEiQ8CH72.

PTM databases

iPTMnetiQ8CH72.
PhosphoSiteiQ8CH72.

Expressioni

Tissue specificityi

Ubiquitous. High expression in brain.

Inductioni

By interferon alpha/UVB treatment.

Gene expression databases

BgeeiQ8CH72.
ExpressionAtlasiQ8CH72. baseline and differential.
GenevisibleiQ8CH72. MM.

Interactioni

Subunit structurei

Interacts with DTNBP1 (By similarity). It self-associates (By similarity). Interacts with PIAS4/PIASY upon treatment with UVB and TNF-alpha.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Ago1Q8CJG12EBI-773837,EBI-2291996
MycP011082EBI-773837,EBI-1183114

GO - Molecular functioni

  • myosin binding Source: MGI
  • protein self-association Source: BHF-UCL
  • translation initiation factor binding Source: MGI
  • ubiquitin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi213691. 16 interactions.
IntActiQ8CH72. 10 interactions.
STRINGi10090.ENSMUSP00000062277.

Structurei

3D structure databases

ProteinModelPortaliQ8CH72.
SMRiQ8CH72. Positions 4-91, 355-634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati360 – 40344NHL 1Add
BLAST
Repeati417 – 46044NHL 2Add
BLAST
Repeati461 – 50141NHL 3Add
BLAST
Repeati564 – 60744NHL 4Add
BLAST
Repeati608 – 64841NHL 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili139 – 19860Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 76Poly-Ala

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 5 NHL repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri21 – 6646RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri96 – 13944B box-type; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFD. Eukaryota.
ENOG410XRCH. LUCA.
GeneTreeiENSGT00550000074377.
HOGENOMiHOG000120114.
HOVERGENiHBG060344.
InParanoidiQ8CH72.
KOiK10607.
OMAiEDFRCIT.
OrthoDBiEOG7BCNB8.
PhylomeDBiQ8CH72.
TreeFamiTF331018.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR033569. TRIM32.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR24103:SF363. PTHR24103:SF363. 2 hits.
PfamiPF01436. NHL. 3 hits.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS51125. NHL. 5 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CH72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAASHL NLDALREVLE CPICMESFTE EQLRPKLLHC GHTICRQCLE
60 70 80 90 100
KLLASSINGV RCPFCSKITR ITSLTQLTDN LTVLKIIDTA GLSEAVGLLM
110 120 130 140 150
CRGCGRRLPR QFCRSCGVVL CEPCREADHQ PPGHCTLPVK EAAEERRRDF
160 170 180 190 200
GEKLTRLREL TGELQRRKAA LEGVSRDLQA RYKAVLQEYG HEERRIQEEL
210 220 230 240 250
ARSRKFFTGS LAEVEKSNSQ VVEEQSYLLN IAEVQAVSRC DYFLAKIKQA
260 270 280 290 300
DVALLEETAD EEEPELTASL PRELTLQDVE LLKVGHVGPL QIGQAVKKPR
310 320 330 340 350
TVNMEDSWAG EEGAASSASA SVTFREMDMS PEEVAPSPRA SPAKQRSSEA
360 370 380 390 400
ASGIQQCLFL KKMGAKGSTP GMFNLPVSLY VTSQSEVLVA DRGNYRIQVF
410 420 430 440 450
NRKGFLKEIR RSPSGIDSFV LSFLGADLPN LTPLSVAMNC HGLIGVTDSY
460 470 480 490 500
DNSLKVYTMD GHCVACHRSQ LSKPWGITAL PSGQFVVTDV EGGKLWCFTV
510 520 530 540 550
DRGAGVVKYS CLCSAVRPKF VTCDAEGTVY FTQGLGLNVE NRQNEHHLEG
560 570 580 590 600
GFSIGSVGPD GQLGRQISHF FSENEDFRCI AGMCVDARGD LIVADSSRKE
610 620 630 640 650
ILHFPKGGGY SVLIREGLTC PVGIALTPKG QLLVLDCWDH CVKIYSYHLR

RYSTP
Length:655
Mass (Da):72,057
Last modified:November 23, 2004 - v2
Checksum:i32C01387DA7E9224
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691A → V in AAO13297 (PubMed:14578165).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347694 mRNA. Translation: AAO13297.1.
AL691456 Genomic DNA. Translation: CAD62276.1.
BC034104 mRNA. Translation: AAH34104.1.
CCDSiCCDS18270.1.
RefSeqiNP_001155254.1. NM_001161782.1.
NP_444314.2. NM_053084.2.
XP_006538298.1. XM_006538235.1.
XP_006538299.1. XM_006538236.2.
XP_006538300.1. XM_006538237.1.
XP_006538301.1. XM_006538238.1.
UniGeneiMm.22786.

Genome annotation databases

EnsembliENSMUST00000050850; ENSMUSP00000062277; ENSMUSG00000051675.
ENSMUST00000107366; ENSMUSP00000102989; ENSMUSG00000051675.
GeneIDi69807.
KEGGimmu:69807.
UCSCiuc008thq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347694 mRNA. Translation: AAO13297.1.
AL691456 Genomic DNA. Translation: CAD62276.1.
BC034104 mRNA. Translation: AAH34104.1.
CCDSiCCDS18270.1.
RefSeqiNP_001155254.1. NM_001161782.1.
NP_444314.2. NM_053084.2.
XP_006538298.1. XM_006538235.1.
XP_006538299.1. XM_006538236.2.
XP_006538300.1. XM_006538237.1.
XP_006538301.1. XM_006538238.1.
UniGeneiMm.22786.

3D structure databases

ProteinModelPortaliQ8CH72.
SMRiQ8CH72. Positions 4-91, 355-634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213691. 16 interactions.
IntActiQ8CH72. 10 interactions.
STRINGi10090.ENSMUSP00000062277.

PTM databases

iPTMnetiQ8CH72.
PhosphoSiteiQ8CH72.

Proteomic databases

EPDiQ8CH72.
MaxQBiQ8CH72.
PaxDbiQ8CH72.
PRIDEiQ8CH72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050850; ENSMUSP00000062277; ENSMUSG00000051675.
ENSMUST00000107366; ENSMUSP00000102989; ENSMUSG00000051675.
GeneIDi69807.
KEGGimmu:69807.
UCSCiuc008thq.2. mouse.

Organism-specific databases

CTDi22954.
MGIiMGI:1917057. Trim32.

Phylogenomic databases

eggNOGiENOG410ITFD. Eukaryota.
ENOG410XRCH. LUCA.
GeneTreeiENSGT00550000074377.
HOGENOMiHOG000120114.
HOVERGENiHBG060344.
InParanoidiQ8CH72.
KOiK10607.
OMAiEDFRCIT.
OrthoDBiEOG7BCNB8.
PhylomeDBiQ8CH72.
TreeFamiTF331018.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-3134975. Regulation of innate immune responses to cytosolic DNA.

Miscellaneous databases

PROiQ8CH72.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CH72.
ExpressionAtlasiQ8CH72. baseline and differential.
GenevisibleiQ8CH72. MM.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR033569. TRIM32.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR24103:SF363. PTHR24103:SF363. 2 hits.
PfamiPF01436. NHL. 3 hits.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
SMARTiSM00336. BBOX. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS51125. NHL. 5 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RING protein Trim32 associated with skin carcinogenesis has anti-apoptotic and E3-ubiquitin ligase properties."
    Horn E.J., Albor A., Liu Y., El-Hizawi S., Vanderbeek G.E., Babcock M., Bowden G.T., Hennings H., Lozano G., Weinberg W.C., Kulesz-Martin M.
    Carcinogenesis 25:157-167(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, UBIQUITINATION.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Retina.
  4. "The interaction of Piasy with Trim32, an E3-ubiquitin ligase mutated in limb-girdle muscular dystrophy type 2H, promotes Piasy degradation and regulates UVB-induced keratinocyte apoptosis through NFkappaB."
    Albor A., El-Hizawi S., Horn E.J., Laederich M., Frosk P., Wrogemann K., Kulesz-Martin M.
    J. Biol. Chem. 281:25850-25866(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PIAS4.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330 AND SER-337, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiTRI32_MOUSE
AccessioniPrimary (citable) accession number: Q8CH72
Secondary accession number(s): Q8K055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 23, 2004
Last modified: July 6, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.