Q8CH18 (CCAR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division cycle and apoptosis regulator protein 1

Alternative name(s):
Cell cycle and apoptosis regulatory protein 1
Short name=CARP-1

Gene names

Name:	Ccar1
Synonyms:	Carp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1146 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the retinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation By similarity. Ref.4
Subunit structure	Directly interacts with CALCOCO1, ESR1, NR3C1 and TP53. Ref.4
Subcellular location	Cytoplasm › perinuclear region By similarity.
Sequence similarities	Contains 1 SAP domain.
Sequence caution	The sequence AAH10199.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH34174.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH39939.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH51052.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH60130.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH79652.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle Transcription Transcription regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ligand-dependent nuclear receptor transcription coactivator activity Inferred from electronic annotation. Source: Compara nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CH18-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CH18-2)

The sequence of this isoform differs from the canonical sequence as follows:
     316-327: SRERDRERRRSR → RYVLRQCGGLEK
     328-1146: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8CH18-3)

The sequence of this isoform differs from the canonical sequence as follows:
     638-699: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1146	1146	Cell division cycle and apoptosis regulator protein 1		PRO_0000233149
Regions
	Domain	633 – 667	35	SAP
	Coiled coil	591 – 615	25	Potential
	Coiled coil	1029 – 1110	82	Potential
	Compositional bias	3 – 287	285	Gln-rich
	Compositional bias	290 – 358	69	Arg-rich
	Compositional bias	670 – 886	217	Glu-rich
Amino acid modifications
	Modified residue	682	1	Phosphoserine By similarity
	Modified residue	694	1	Phosphoserine By similarity
	Modified residue	858	1	Phosphothreonine By similarity
	Cross-link	634		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Natural variations
	Alternative sequence	316 – 327	12	SRERD…RRRSR → RYVLRQCGGLEK in isoform 2.		VSP_018054
	Alternative sequence	328 – 1146	819	Missing in isoform 2.		VSP_018055
	Alternative sequence	638 – 699	62	Missing in isoform 3.		VSP_037737
Experimental info
	Sequence conflict	279	1	P → L in AAH79652. Ref.3
	Sequence conflict	558	1	T → A in AAH39939. Ref.3
	Sequence conflict	802	1	K → E in BAC37267. Ref.2
	Sequence conflict	821	1	E → G in BAC37267. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6005A057B4F0BFF0	FASTA	1,146	132,060
	10 20 30 40 50 60 MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA AGLTTQTPAN 70 80 90 100 110 120 YQLTQTAALQ QQAAAVLQQQ YSQPQQALYS VQQQLQQPQQ TILTQPAVAL PTSLSLSTPQ 130 140 150 160 170 180 PAAQITVSYP TPRSSQQQTQ PQKQRVFTGV VTKLHDTFGF VDEDVFFQLG AVKGKTPQVG 190 200 210 220 230 240 DRVLVEATYN PNMPFKWNAQ RIQTLPNQNQ SQTQPLLKTP TAVIQPIVPQ TTFGVQAQPQ 250 260 270 280 290 300 PQSLLQAQIS AASITPLLQT QPQPLLQQPQ QKAGLLQPPV RIVSQPQPAR RLDPPSRFSG 310 320 330 340 350 360 RNDRGDQVPN RKDDRSRERD RERRRSRERS PQRKRSRERS PRRERERSPR RVRRVVPRYT 370 380 390 400 410 420 VQFSKFSLDC PSCDMMELRR RYQNLYIPSD FFDAQFTWVD AFPLSRPFQL GNYCNFYVMH 430 440 450 460 470 480 REVESLEKNM AVLDPPDADH LYSAKVMLMA SPSMEDLYHK SCALAEDPQD LRDGFQHPAR 490 500 510 520 530 540 LVKFLVGMKG KDEAMAIGGH WSPSLDGPNP EKDPSVLIKT AIRCCKALTG IDLSVCTQWY 550 560 570 580 590 600 RFAEIRYHRP EETHKGRTVP AHVETVVLFF PDVWHCLPTR SEWETLSRGY KQQLVEKLQG 610 620 630 640 650 660 ERKKADGEQD EEEKDDGEVK EIATPTHWSK LDPKAMKVND LRKELESRAL SSKGLKSQLI 670 680 690 700 710 720 ARLTKQLKIE EQKEEQKELE KSEKEEEDED DKKSEDDKEE EERKRQEEVE RQRQERRYIL 730 740 750 760 770 780 PDEPAIIVHP NWAAKSGKFD CSIMSLSVLL DYRLEDNKEH SFEVSLFAEL FNEMLQRDFG 790 800 810 820 830 840 VRIYKSLLSL PEKEDKKDKE KKSKKEERKD KKEEREDDID EPKPKRRKSG DDKDKKEDRD 850 860 870 880 890 900 ERKKEEKRKD DSKDDDETEE DNNQDEYDPM EAEEAEDEDD DREEEEVKRD DKRDVSRYCK 910 920 930 940 950 960 DRPAKDKEKE KPQMVTVNRD LLMAFVYFDQ SHCGYLLEKD LEEILYTLGL HLSRAQVKKL 970 980 990 1000 1010 1020 LNKVVLRESC FYRKLTDTSK DDENHEESEA LQEDMLGNRL LLPTPTIKQE SKDGEENVGL 1030 1040 1050 1060 1070 1080 IVYNGAMVDV GSLLQKLEKS EKVRAEVEQK LQLLEEKTDE DGKTILNLEN SNKSLSGELR 1090 1100 1110 1120 1130 1140 EVKKDLGQLQ ENLEVSENMN LQFENQLNKT LRNLSTVMDD IHTVLKKDNV KSEDRDEKSK ENGSGV
	Isoform 2 [UniParc]. Checksum: 42FF713BD7603559	FASTA	327	35,718
	10 20 30 40 50 60 MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA AGLTTQTPAN 70 80 90 100 110 120 YQLTQTAALQ QQAAAVLQQQ YSQPQQALYS VQQQLQQPQQ TILTQPAVAL PTSLSLSTPQ 130 140 150 160 170 180 PAAQITVSYP TPRSSQQQTQ PQKQRVFTGV VTKLHDTFGF VDEDVFFQLG AVKGKTPQVG 190 200 210 220 230 240 DRVLVEATYN PNMPFKWNAQ RIQTLPNQNQ SQTQPLLKTP TAVIQPIVPQ TTFGVQAQPQ 250 260 270 280 290 300 PQSLLQAQIS AASITPLLQT QPQPLLQQPQ QKAGLLQPPV RIVSQPQPAR RLDPPSRFSG 310 320 RNDRGDQVPN RKDDRRYVLR QCGGLEK
	Isoform 3 [UniParc]. Checksum: AB6C39FC58378B7C	FASTA	1,084	124,746
	10 20 30 40 50 60 MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA AGLTTQTPAN 70 80 90 100 110 120 YQLTQTAALQ QQAAAVLQQQ YSQPQQALYS VQQQLQQPQQ TILTQPAVAL PTSLSLSTPQ 130 140 150 160 170 180 PAAQITVSYP TPRSSQQQTQ PQKQRVFTGV VTKLHDTFGF VDEDVFFQLG AVKGKTPQVG 190 200 210 220 230 240 DRVLVEATYN PNMPFKWNAQ RIQTLPNQNQ SQTQPLLKTP TAVIQPIVPQ TTFGVQAQPQ 250 260 270 280 290 300 PQSLLQAQIS AASITPLLQT QPQPLLQQPQ QKAGLLQPPV RIVSQPQPAR RLDPPSRFSG 310 320 330 340 350 360 RNDRGDQVPN RKDDRSRERD RERRRSRERS PQRKRSRERS PRRERERSPR RVRRVVPRYT 370 380 390 400 410 420 VQFSKFSLDC PSCDMMELRR RYQNLYIPSD FFDAQFTWVD AFPLSRPFQL GNYCNFYVMH 430 440 450 460 470 480 REVESLEKNM AVLDPPDADH LYSAKVMLMA SPSMEDLYHK SCALAEDPQD LRDGFQHPAR 490 500 510 520 530 540 LVKFLVGMKG KDEAMAIGGH WSPSLDGPNP EKDPSVLIKT AIRCCKALTG IDLSVCTQWY 550 560 570 580 590 600 RFAEIRYHRP EETHKGRTVP AHVETVVLFF PDVWHCLPTR SEWETLSRGY KQQLVEKLQG 610 620 630 640 650 660 ERKKADGEQD EEEKDDGEVK EIATPTHWSK LDPKAMKEEE RKRQEEVERQ RQERRYILPD 670 680 690 700 710 720 EPAIIVHPNW AAKSGKFDCS IMSLSVLLDY RLEDNKEHSF EVSLFAELFN EMLQRDFGVR 730 740 750 760 770 780 IYKSLLSLPE KEDKKDKEKK SKKEERKDKK EEREDDIDEP KPKRRKSGDD KDKKEDRDER 790 800 810 820 830 840 KKEEKRKDDS KDDDETEEDN NQDEYDPMEA EEAEDEDDDR EEEEVKRDDK RDVSRYCKDR 850 860 870 880 890 900 PAKDKEKEKP QMVTVNRDLL MAFVYFDQSH CGYLLEKDLE EILYTLGLHL SRAQVKKLLN 910 920 930 940 950 960 KVVLRESCFY RKLTDTSKDD ENHEESEALQ EDMLGNRLLL PTPTIKQESK DGEENVGLIV 970 980 990 1000 1010 1020 YNGAMVDVGS LLQKLEKSEK VRAEVEQKLQ LLEEKTDEDG KTILNLENSN KSLSGELREV 1030 1040 1050 1060 1070 1080 KKDLGQLQEN LEVSENMNLQ FENQLNKTLR NLSTVMDDIH TVLKKDNVKS EDRDEKSKEN GSGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TAZ binding partners identified by mass spectrometry." Tian Y., Li D., Benjamin T. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Swiss Webster.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Embryo, Testis and Wolffian duct.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-833 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-797 (ISOFORM 3). Strain: C57BL/6, Czech II and FVB/N. Tissue: Brain, Kidney and Mammary tumor.
[4]	"CCAR1, a key regulator of mediator complex recruitment to nuclear receptor transcription complexes." Kim J.H., Yang C.K., Heo K., Roeder R.G., An W., Stallcup M.R. Mol. Cell 31:510-519(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CALCOCO1; ESR1 AND NR3C.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF465615 mRNA. Translation: AAO17318.1. AK012111 mRNA. Translation: BAB28040.2. AK077151 mRNA. Translation: BAC36646.1. AK078424 mRNA. Translation: BAC37267.1. BC010199 mRNA. Translation: AAH10199.1. Sequence problems. BC034174 mRNA. Translation: AAH34174.1. Sequence problems. BC039939 mRNA. Translation: AAH39939.1. Sequence problems. BC051052 mRNA. Translation: AAH51052.1. Sequence problems. BC060130 mRNA. Translation: AAH60130.1. Sequence problems. BC079652 mRNA. Translation: AAH79652.1. Sequence problems.
IPI	IPI00135207. IPI00753725. IPI00943440.
RefSeq	NP_080477.1. NM_026201.3.
UniGene	Mm.196371.
3D structure databases
ProteinModelPortal	Q8CH18.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CH18. 2 interactions.
PTM databases
PhosphoSite	Q8CH18.
Proteomic databases
PaxDb	Q8CH18.
PRIDE	Q8CH18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020268; ENSMUSP00000020268; ENSMUSG00000020074.
GeneID	67500.
KEGG	mmu:67500.
UCSC	uc007fja.2. mouse. uc007fjc.2. mouse. uc011xff.1. mouse.
Organism-specific databases
CTD	55749.
MGI	MGI:1914750. Ccar1.
Phylogenomic databases
eggNOG	NOG313311.
GeneTree	ENSGT00530000063672.
HOVERGEN	HBG079908.
InParanoid	Q05BR1.
OMA	MITVNRD.
OrthoDB	EOG4CC410.
Gene expression databases
Bgee	Q8CH18.
CleanEx	MM_CCAR1.
Genevestigator	Q8CH18.
GermOnline	ENSMUSG00000020074. Mus musculus.
Family and domain databases
Gene3D	1.10.720.30. 1 hit.
InterPro	IPR025224. DBC1/CARP1. IPR025954. DBC1/CARP1_inactive_NUDIX_dom. IPR012340. NA-bd_OB-fold. IPR025223. S1-like_RNA-bd_dom. IPR003034. SAP_dom. [Graphical view]
PANTHER	PTHR14304. PTHR14304. 1 hit.
Pfam	PF14443. DBC1. 1 hit. PF14444. S1-like. 1 hit. PF02037. SAP. 1 hit. [Graphical view]
SMART	SM00513. SAP. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS50800. SAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CCAR1. mouse.
NextBio	324750.
SOURCE	Search...

Entry information

Entry name

CCAR1_MOUSE

Accession

Primary (citable) accession number: Q8CH18
Secondary accession number(s): Q05BR1 , Q05DK6, Q6AXC9, Q6PAR2, Q80XE4, Q8BJY0, Q8BVN2, Q8CGG1, Q9CSR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program