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Protein

Cell division cycle and apoptosis regulator protein 1

Gene

Ccar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the retinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (By similarity).By similarity

GO - Molecular functioni

  1. ligand-dependent nuclear receptor transcription coactivator activity Source: MGI
  2. poly(A) RNA binding Source: MGI

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cell cycle Source: UniProtKB-KW
  3. positive regulation of apoptotic process Source: MGI
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_307866. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle and apoptosis regulator protein 1
Alternative name(s):
Cell cycle and apoptosis regulatory protein 1
Short name:
CARP-1
Gene namesi
Name:Ccar1
Synonyms:Carp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1914750. Ccar1.

Subcellular locationi

Cytoplasmperinuclear region By similarity

GO - Cellular componenti

  1. nuclear envelope lumen Source: MGI
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11461146Cell division cycle and apoptosis regulator protein 1PRO_0000233149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki634 – 634Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei664 – 6641PhosphothreonineBy similarity
Modified residuei682 – 6821PhosphoserineBy similarity
Modified residuei694 – 6941PhosphoserineBy similarity
Modified residuei858 – 8581PhosphothreonineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8CH18.
PaxDbiQ8CH18.
PRIDEiQ8CH18.

PTM databases

PhosphoSiteiQ8CH18.

Expressioni

Gene expression databases

BgeeiQ8CH18.
CleanExiMM_CCAR1.
GenevestigatoriQ8CH18.

Interactioni

Subunit structurei

Directly interacts with CALCOCO1, ESR1, NR3C1 and TP53.1 Publication

Protein-protein interaction databases

BioGridi212231. 6 interactions.
IntActiQ8CH18. 3 interactions.
MINTiMINT-1550931.

Structurei

3D structure databases

ProteinModelPortaliQ8CH18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini633 – 66735SAPPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili591 – 61525Sequence AnalysisAdd
BLAST
Coiled coili1029 – 111082Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 287285Gln-richAdd
BLAST
Compositional biasi290 – 35869Arg-richAdd
BLAST
Compositional biasi670 – 886217Glu-richAdd
BLAST

Sequence similaritiesi

Contains 1 SAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG313311.
GeneTreeiENSGT00530000063672.
HOVERGENiHBG079908.
InParanoidiQ8CH18.
OMAiPNWAAKN.
OrthoDBiEOG7CK365.
PhylomeDBiQ8CH18.
TreeFamiTF316387.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR025224. CCAR1/CCAR2.
IPR025954. DBC1/CARP1_inactive_NUDIX_dom.
IPR025223. S1-like_RNA-bd_dom.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR14304. PTHR14304. 1 hit.
PfamiPF14443. DBC1. 1 hit.
PF14444. S1-like. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CH18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA
60 70 80 90 100
AGLTTQTPAN YQLTQTAALQ QQAAAVLQQQ YSQPQQALYS VQQQLQQPQQ
110 120 130 140 150
TILTQPAVAL PTSLSLSTPQ PAAQITVSYP TPRSSQQQTQ PQKQRVFTGV
160 170 180 190 200
VTKLHDTFGF VDEDVFFQLG AVKGKTPQVG DRVLVEATYN PNMPFKWNAQ
210 220 230 240 250
RIQTLPNQNQ SQTQPLLKTP TAVIQPIVPQ TTFGVQAQPQ PQSLLQAQIS
260 270 280 290 300
AASITPLLQT QPQPLLQQPQ QKAGLLQPPV RIVSQPQPAR RLDPPSRFSG
310 320 330 340 350
RNDRGDQVPN RKDDRSRERD RERRRSRERS PQRKRSRERS PRRERERSPR
360 370 380 390 400
RVRRVVPRYT VQFSKFSLDC PSCDMMELRR RYQNLYIPSD FFDAQFTWVD
410 420 430 440 450
AFPLSRPFQL GNYCNFYVMH REVESLEKNM AVLDPPDADH LYSAKVMLMA
460 470 480 490 500
SPSMEDLYHK SCALAEDPQD LRDGFQHPAR LVKFLVGMKG KDEAMAIGGH
510 520 530 540 550
WSPSLDGPNP EKDPSVLIKT AIRCCKALTG IDLSVCTQWY RFAEIRYHRP
560 570 580 590 600
EETHKGRTVP AHVETVVLFF PDVWHCLPTR SEWETLSRGY KQQLVEKLQG
610 620 630 640 650
ERKKADGEQD EEEKDDGEVK EIATPTHWSK LDPKAMKVND LRKELESRAL
660 670 680 690 700
SSKGLKSQLI ARLTKQLKIE EQKEEQKELE KSEKEEEDED DKKSEDDKEE
710 720 730 740 750
EERKRQEEVE RQRQERRYIL PDEPAIIVHP NWAAKSGKFD CSIMSLSVLL
760 770 780 790 800
DYRLEDNKEH SFEVSLFAEL FNEMLQRDFG VRIYKSLLSL PEKEDKKDKE
810 820 830 840 850
KKSKKEERKD KKEEREDDID EPKPKRRKSG DDKDKKEDRD ERKKEEKRKD
860 870 880 890 900
DSKDDDETEE DNNQDEYDPM EAEEAEDEDD DREEEEVKRD DKRDVSRYCK
910 920 930 940 950
DRPAKDKEKE KPQMVTVNRD LLMAFVYFDQ SHCGYLLEKD LEEILYTLGL
960 970 980 990 1000
HLSRAQVKKL LNKVVLRESC FYRKLTDTSK DDENHEESEA LQEDMLGNRL
1010 1020 1030 1040 1050
LLPTPTIKQE SKDGEENVGL IVYNGAMVDV GSLLQKLEKS EKVRAEVEQK
1060 1070 1080 1090 1100
LQLLEEKTDE DGKTILNLEN SNKSLSGELR EVKKDLGQLQ ENLEVSENMN
1110 1120 1130 1140
LQFENQLNKT LRNLSTVMDD IHTVLKKDNV KSEDRDEKSK ENGSGV
Length:1,146
Mass (Da):132,060
Last modified:March 1, 2003 - v1
Checksum:i6005A057B4F0BFF0
GO
Isoform 2 (identifier: Q8CH18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-327: SRERDRERRRSR → RYVLRQCGGLEK
     328-1146: Missing.

Note: No experimental confirmation available.

Show »
Length:327
Mass (Da):35,718
Checksum:i42FF713BD7603559
GO
Isoform 3 (identifier: Q8CH18-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     638-699: Missing.

Note: No experimental confirmation available.

Show »
Length:1,084
Mass (Da):124,746
Checksum:iAB6C39FC58378B7C
GO

Sequence cautioni

The sequence AAH10199.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH34174.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH39939.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH51052.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH60130.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH79652.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti279 – 2791P → L in AAH79652 (PubMed:15489334).Curated
Sequence conflicti558 – 5581T → A in AAH39939 (PubMed:15489334).Curated
Sequence conflicti802 – 8021K → E in BAC37267 (PubMed:16141072).Curated
Sequence conflicti821 – 8211E → G in BAC37267 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei316 – 32712SRERD…RRRSR → RYVLRQCGGLEK in isoform 2. 1 PublicationVSP_018054Add
BLAST
Alternative sequencei328 – 1146819Missing in isoform 2. 1 PublicationVSP_018055Add
BLAST
Alternative sequencei638 – 69962Missing in isoform 3. 1 PublicationVSP_037737Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465615 mRNA. Translation: AAO17318.1.
AK012111 mRNA. Translation: BAB28040.2.
AK077151 mRNA. Translation: BAC36646.1.
AK078424 mRNA. Translation: BAC37267.1.
BC010199 mRNA. Translation: AAH10199.1. Sequence problems.
BC034174 mRNA. Translation: AAH34174.1. Sequence problems.
BC039939 mRNA. Translation: AAH39939.1. Sequence problems.
BC051052 mRNA. Translation: AAH51052.1. Sequence problems.
BC060130 mRNA. Translation: AAH60130.1. Sequence problems.
BC079652 mRNA. Translation: AAH79652.1. Sequence problems.
CCDSiCCDS35922.1. [Q8CH18-1]
RefSeqiNP_080477.1. NM_026201.3. [Q8CH18-1]
XP_006514057.1. XM_006513994.2. [Q8CH18-1]
XP_006514058.1. XM_006513995.2. [Q8CH18-1]
XP_006514059.1. XM_006513996.2. [Q8CH18-1]
XP_006514060.1. XM_006513997.1. [Q8CH18-3]
UniGeneiMm.196371.

Genome annotation databases

EnsembliENSMUST00000020268; ENSMUSP00000020268; ENSMUSG00000020074. [Q8CH18-1]
GeneIDi67500.
KEGGimmu:67500.
UCSCiuc007fja.2. mouse. [Q8CH18-1]
uc007fjc.2. mouse. [Q8CH18-2]
uc011xff.1. mouse. [Q8CH18-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465615 mRNA. Translation: AAO17318.1.
AK012111 mRNA. Translation: BAB28040.2.
AK077151 mRNA. Translation: BAC36646.1.
AK078424 mRNA. Translation: BAC37267.1.
BC010199 mRNA. Translation: AAH10199.1. Sequence problems.
BC034174 mRNA. Translation: AAH34174.1. Sequence problems.
BC039939 mRNA. Translation: AAH39939.1. Sequence problems.
BC051052 mRNA. Translation: AAH51052.1. Sequence problems.
BC060130 mRNA. Translation: AAH60130.1. Sequence problems.
BC079652 mRNA. Translation: AAH79652.1. Sequence problems.
CCDSiCCDS35922.1. [Q8CH18-1]
RefSeqiNP_080477.1. NM_026201.3. [Q8CH18-1]
XP_006514057.1. XM_006513994.2. [Q8CH18-1]
XP_006514058.1. XM_006513995.2. [Q8CH18-1]
XP_006514059.1. XM_006513996.2. [Q8CH18-1]
XP_006514060.1. XM_006513997.1. [Q8CH18-3]
UniGeneiMm.196371.

3D structure databases

ProteinModelPortaliQ8CH18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212231. 6 interactions.
IntActiQ8CH18. 3 interactions.
MINTiMINT-1550931.

PTM databases

PhosphoSiteiQ8CH18.

Proteomic databases

MaxQBiQ8CH18.
PaxDbiQ8CH18.
PRIDEiQ8CH18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020268; ENSMUSP00000020268; ENSMUSG00000020074. [Q8CH18-1]
GeneIDi67500.
KEGGimmu:67500.
UCSCiuc007fja.2. mouse. [Q8CH18-1]
uc007fjc.2. mouse. [Q8CH18-2]
uc011xff.1. mouse. [Q8CH18-3]

Organism-specific databases

CTDi55749.
MGIiMGI:1914750. Ccar1.

Phylogenomic databases

eggNOGiNOG313311.
GeneTreeiENSGT00530000063672.
HOVERGENiHBG079908.
InParanoidiQ8CH18.
OMAiPNWAAKN.
OrthoDBiEOG7CK365.
PhylomeDBiQ8CH18.
TreeFamiTF316387.

Enzyme and pathway databases

ReactomeiREACT_307866. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiCcar1. mouse.
NextBioi324750.
PROiQ8CH18.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CH18.
CleanExiMM_CCAR1.
GenevestigatoriQ8CH18.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR025224. CCAR1/CCAR2.
IPR025954. DBC1/CARP1_inactive_NUDIX_dom.
IPR025223. S1-like_RNA-bd_dom.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR14304. PTHR14304. 1 hit.
PfamiPF14443. DBC1. 1 hit.
PF14444. S1-like. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TAZ binding partners identified by mass spectrometry."
    Tian Y., Li D., Benjamin T.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Swiss Webster.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryo, Testis and Wolffian duct.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-833 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-797 (ISOFORM 3).
    Strain: C57BL/6, Czech II and FVB/N.
    Tissue: Brain, Kidney and Mammary tumor.
  4. "CCAR1, a key regulator of mediator complex recruitment to nuclear receptor transcription complexes."
    Kim J.H., Yang C.K., Heo K., Roeder R.G., An W., Stallcup M.R.
    Mol. Cell 31:510-519(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CALCOCO1; ESR1 AND NR3C.

Entry informationi

Entry nameiCCAR1_MOUSE
AccessioniPrimary (citable) accession number: Q8CH18
Secondary accession number(s): Q05BR1
, Q05DK6, Q6AXC9, Q6PAR2, Q80XE4, Q8BJY0, Q8BVN2, Q8CGG1, Q9CSR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.