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Protein

Cell division cycle and apoptosis regulator protein 1

Gene

Ccar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the retinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (By similarity). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Apoptosis, Cell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle and apoptosis regulator protein 1
Alternative name(s):
Cell cycle and apoptosis regulatory protein 1
Short name:
CARP-1
Gene namesi
Name:Ccar1
Synonyms:Carp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1914750. Ccar1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002331491 – 1146Cell division cycle and apoptosis regulator protein 1Add BLAST1146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei453PhosphoserineBy similarity1
Modified residuei624PhosphothreonineBy similarity1
Cross-linki634Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei664PhosphothreonineBy similarity1
Modified residuei682PhosphoserineBy similarity1
Modified residuei694PhosphoserineBy similarity1
Modified residuei858PhosphothreonineBy similarity1
Cross-linki1008Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki1008Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1063Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki1131Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CH18.
PaxDbiQ8CH18.
PeptideAtlasiQ8CH18.
PRIDEiQ8CH18.

PTM databases

iPTMnetiQ8CH18.
PhosphoSitePlusiQ8CH18.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020074.
CleanExiMM_CCAR1.
GenevisibleiQ8CH18. MM.

Interactioni

Subunit structurei

Directly interacts with ESR1, NR3C1 and p53/TP53. Interacts (via N-terminus) with CALCOCO1. Interacts with MED1 and GATA1. Interacts with AR and GATA2 (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi212231. 6 interactors.
IntActiQ8CH18. 3 interactors.
MINTiMINT-1550931.
STRINGi10090.ENSMUSP00000020268.

Structurei

3D structure databases

ProteinModelPortaliQ8CH18.
SMRiQ8CH18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini633 – 667SAPPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 246Interaction with ARBy similarityAdd BLAST246
Regioni200 – 657Interaction with GATA2By similarityAdd BLAST458
Regioni640 – 1146Interaction with GATA11 PublicationAdd BLAST507

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili591 – 615Sequence analysisAdd BLAST25
Coiled coili1029 – 1110Sequence analysisAdd BLAST82

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 287Gln-richAdd BLAST285
Compositional biasi290 – 358Arg-richAdd BLAST69
Compositional biasi670 – 886Glu-richAdd BLAST217

Sequence similaritiesi

Contains 1 SAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4246. Eukaryota.
ENOG410Z0UJ. LUCA.
GeneTreeiENSGT00530000063672.
HOVERGENiHBG079908.
InParanoidiQ8CH18.
OMAiHPARLIK.
OrthoDBiEOG091G03QV.
PhylomeDBiQ8CH18.
TreeFamiTF316387.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR025224. CCAR1/CCAR2.
IPR025954. DBC1/CARP1_inactive_NUDIX_dom.
IPR011992. EF-hand-dom_pair.
IPR025223. S1-like_RNA-bd_dom.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR14304. PTHR14304. 2 hits.
PfamiPF14443. DBC1. 1 hit.
PF14444. S1-like. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM01122. DBC1. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CH18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA
60 70 80 90 100
AGLTTQTPAN YQLTQTAALQ QQAAAVLQQQ YSQPQQALYS VQQQLQQPQQ
110 120 130 140 150
TILTQPAVAL PTSLSLSTPQ PAAQITVSYP TPRSSQQQTQ PQKQRVFTGV
160 170 180 190 200
VTKLHDTFGF VDEDVFFQLG AVKGKTPQVG DRVLVEATYN PNMPFKWNAQ
210 220 230 240 250
RIQTLPNQNQ SQTQPLLKTP TAVIQPIVPQ TTFGVQAQPQ PQSLLQAQIS
260 270 280 290 300
AASITPLLQT QPQPLLQQPQ QKAGLLQPPV RIVSQPQPAR RLDPPSRFSG
310 320 330 340 350
RNDRGDQVPN RKDDRSRERD RERRRSRERS PQRKRSRERS PRRERERSPR
360 370 380 390 400
RVRRVVPRYT VQFSKFSLDC PSCDMMELRR RYQNLYIPSD FFDAQFTWVD
410 420 430 440 450
AFPLSRPFQL GNYCNFYVMH REVESLEKNM AVLDPPDADH LYSAKVMLMA
460 470 480 490 500
SPSMEDLYHK SCALAEDPQD LRDGFQHPAR LVKFLVGMKG KDEAMAIGGH
510 520 530 540 550
WSPSLDGPNP EKDPSVLIKT AIRCCKALTG IDLSVCTQWY RFAEIRYHRP
560 570 580 590 600
EETHKGRTVP AHVETVVLFF PDVWHCLPTR SEWETLSRGY KQQLVEKLQG
610 620 630 640 650
ERKKADGEQD EEEKDDGEVK EIATPTHWSK LDPKAMKVND LRKELESRAL
660 670 680 690 700
SSKGLKSQLI ARLTKQLKIE EQKEEQKELE KSEKEEEDED DKKSEDDKEE
710 720 730 740 750
EERKRQEEVE RQRQERRYIL PDEPAIIVHP NWAAKSGKFD CSIMSLSVLL
760 770 780 790 800
DYRLEDNKEH SFEVSLFAEL FNEMLQRDFG VRIYKSLLSL PEKEDKKDKE
810 820 830 840 850
KKSKKEERKD KKEEREDDID EPKPKRRKSG DDKDKKEDRD ERKKEEKRKD
860 870 880 890 900
DSKDDDETEE DNNQDEYDPM EAEEAEDEDD DREEEEVKRD DKRDVSRYCK
910 920 930 940 950
DRPAKDKEKE KPQMVTVNRD LLMAFVYFDQ SHCGYLLEKD LEEILYTLGL
960 970 980 990 1000
HLSRAQVKKL LNKVVLRESC FYRKLTDTSK DDENHEESEA LQEDMLGNRL
1010 1020 1030 1040 1050
LLPTPTIKQE SKDGEENVGL IVYNGAMVDV GSLLQKLEKS EKVRAEVEQK
1060 1070 1080 1090 1100
LQLLEEKTDE DGKTILNLEN SNKSLSGELR EVKKDLGQLQ ENLEVSENMN
1110 1120 1130 1140
LQFENQLNKT LRNLSTVMDD IHTVLKKDNV KSEDRDEKSK ENGSGV
Length:1,146
Mass (Da):132,060
Last modified:March 1, 2003 - v1
Checksum:i6005A057B4F0BFF0
GO
Isoform 2 (identifier: Q8CH18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-327: SRERDRERRRSR → RYVLRQCGGLEK
     328-1146: Missing.

Note: No experimental confirmation available.
Show »
Length:327
Mass (Da):35,718
Checksum:i42FF713BD7603559
GO
Isoform 3 (identifier: Q8CH18-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     638-699: Missing.

Note: No experimental confirmation available.
Show »
Length:1,084
Mass (Da):124,746
Checksum:iAB6C39FC58378B7C
GO

Sequence cautioni

The sequence AAH10199 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH34174 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH39939 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH51052 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH60130 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH79652 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279P → L in AAH79652 (PubMed:15489334).Curated1
Sequence conflicti558T → A in AAH39939 (PubMed:15489334).Curated1
Sequence conflicti802K → E in BAC37267 (PubMed:16141072).Curated1
Sequence conflicti821E → G in BAC37267 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018054316 – 327SRERD…RRRSR → RYVLRQCGGLEK in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_018055328 – 1146Missing in isoform 2. 1 PublicationAdd BLAST819
Alternative sequenceiVSP_037737638 – 699Missing in isoform 3. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465615 mRNA. Translation: AAO17318.1.
AK012111 mRNA. Translation: BAB28040.2.
AK077151 mRNA. Translation: BAC36646.1.
AK078424 mRNA. Translation: BAC37267.1.
BC010199 mRNA. Translation: AAH10199.1. Sequence problems.
BC034174 mRNA. Translation: AAH34174.1. Sequence problems.
BC039939 mRNA. Translation: AAH39939.1. Sequence problems.
BC051052 mRNA. Translation: AAH51052.1. Sequence problems.
BC060130 mRNA. Translation: AAH60130.1. Sequence problems.
BC079652 mRNA. Translation: AAH79652.1. Sequence problems.
CCDSiCCDS35922.1. [Q8CH18-1]
RefSeqiNP_080477.1. NM_026201.3. [Q8CH18-1]
XP_006514057.1. XM_006513994.3. [Q8CH18-1]
XP_006514058.1. XM_006513995.3. [Q8CH18-1]
XP_006514059.1. XM_006513996.3. [Q8CH18-1]
XP_006514060.1. XM_006513997.2. [Q8CH18-3]
XP_011241839.1. XM_011243537.2. [Q8CH18-1]
UniGeneiMm.196371.

Genome annotation databases

EnsembliENSMUST00000020268; ENSMUSP00000020268; ENSMUSG00000020074. [Q8CH18-1]
GeneIDi67500.
KEGGimmu:67500.
UCSCiuc007fja.2. mouse. [Q8CH18-1]
uc007fjc.2. mouse. [Q8CH18-2]
uc011xff.1. mouse. [Q8CH18-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465615 mRNA. Translation: AAO17318.1.
AK012111 mRNA. Translation: BAB28040.2.
AK077151 mRNA. Translation: BAC36646.1.
AK078424 mRNA. Translation: BAC37267.1.
BC010199 mRNA. Translation: AAH10199.1. Sequence problems.
BC034174 mRNA. Translation: AAH34174.1. Sequence problems.
BC039939 mRNA. Translation: AAH39939.1. Sequence problems.
BC051052 mRNA. Translation: AAH51052.1. Sequence problems.
BC060130 mRNA. Translation: AAH60130.1. Sequence problems.
BC079652 mRNA. Translation: AAH79652.1. Sequence problems.
CCDSiCCDS35922.1. [Q8CH18-1]
RefSeqiNP_080477.1. NM_026201.3. [Q8CH18-1]
XP_006514057.1. XM_006513994.3. [Q8CH18-1]
XP_006514058.1. XM_006513995.3. [Q8CH18-1]
XP_006514059.1. XM_006513996.3. [Q8CH18-1]
XP_006514060.1. XM_006513997.2. [Q8CH18-3]
XP_011241839.1. XM_011243537.2. [Q8CH18-1]
UniGeneiMm.196371.

3D structure databases

ProteinModelPortaliQ8CH18.
SMRiQ8CH18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212231. 6 interactors.
IntActiQ8CH18. 3 interactors.
MINTiMINT-1550931.
STRINGi10090.ENSMUSP00000020268.

PTM databases

iPTMnetiQ8CH18.
PhosphoSitePlusiQ8CH18.

Proteomic databases

EPDiQ8CH18.
PaxDbiQ8CH18.
PeptideAtlasiQ8CH18.
PRIDEiQ8CH18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020268; ENSMUSP00000020268; ENSMUSG00000020074. [Q8CH18-1]
GeneIDi67500.
KEGGimmu:67500.
UCSCiuc007fja.2. mouse. [Q8CH18-1]
uc007fjc.2. mouse. [Q8CH18-2]
uc011xff.1. mouse. [Q8CH18-3]

Organism-specific databases

CTDi55749.
MGIiMGI:1914750. Ccar1.

Phylogenomic databases

eggNOGiKOG4246. Eukaryota.
ENOG410Z0UJ. LUCA.
GeneTreeiENSGT00530000063672.
HOVERGENiHBG079908.
InParanoidiQ8CH18.
OMAiHPARLIK.
OrthoDBiEOG091G03QV.
PhylomeDBiQ8CH18.
TreeFamiTF316387.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiCcar1. mouse.
PROiQ8CH18.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020074.
CleanExiMM_CCAR1.
GenevisibleiQ8CH18. MM.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR025224. CCAR1/CCAR2.
IPR025954. DBC1/CARP1_inactive_NUDIX_dom.
IPR011992. EF-hand-dom_pair.
IPR025223. S1-like_RNA-bd_dom.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR14304. PTHR14304. 2 hits.
PfamiPF14443. DBC1. 1 hit.
PF14444. S1-like. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM01122. DBC1. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCCAR1_MOUSE
AccessioniPrimary (citable) accession number: Q8CH18
Secondary accession number(s): Q05BR1
, Q05DK6, Q6AXC9, Q6PAR2, Q80XE4, Q8BJY0, Q8BVN2, Q8CGG1, Q9CSR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.