Q8CH02 (SUGP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SURP and G-patch domain-containing protein 1

Alternative name(s):
Splicing factor 4

Gene names

Name:	Sugp1
Synonyms:	Sf4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in pre-mRNA splicing By similarity.
Subunit structure	Component of the spliceosome By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 G-patch domain. Contains 2 SURP motif repeats.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	spliceosomal complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 643

643

SURP and G-patch domain-containing protein 1

PRO_0000097702

Regions

Repeat

187 – 229

43

SURP motif 1

Repeat

262 – 305

44

SURP motif 2

Domain

560 – 607

48

G-patch

Motif

378 – 384

7

Nuclear localization signal Potential

Compositional bias

324 – 371

48

Pro-rich

Compositional bias

439 – 478

40

Gln/Met-rich

Amino acid modifications

Modified residue

407

1

Phosphoserine By similarity

Modified residue

409

1

Phosphoserine By similarity

Modified residue

412

1

Phosphoserine By similarity

Modified residue

483

1

Phosphoserine Ref.4

Experimental info

Sequence conflict

326

1

P → L in AAI20920. Ref.3

Sequence conflict

326

1

P → L in AAI20921. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

643

Helix Strand Turn

Details...

Helix

167 – 172

6

Helix

179 – 181

3

Helix

182 – 198

17

Helix

200 – 209

10

Turn

210 – 212

3

Beta strand

214 – 216

3

Helix

217 – 220

4

Helix

225 – 239

15

Helix

257 – 272

16

Helix

277 – 287

11

Turn

291 – 295

5

Beta strand

297 – 300

4

Helix

301 – 313

13

Sequences

	Sequence		Length	Mass (Da)
	Q8CH02 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A1FCD38A26998E78	FASTA	643	72,649
	10 20 30 40 50 60 MSLKMDNRDV AGKANRWFGM AQPKSGKMNM NILHQEELIA QKKREIEARM EQKARQSHVP 70 80 90 100 110 120 SPQPPHPGEI ADAHNSCISN KFANDGSFLQ QFLKLQKAQT STDSAPRAPP SMPTPSSLKK 130 140 150 160 170 180 PLVLSKRTGL GLSSPTGPVK NYSHAKQLPV AHRPSVFQSP DDDEEEDYEQ WLEIKVSPPE 190 200 210 220 230 240 GAETRRVIEK LARFVAEGGP ELEKVAMEDY KDNPAFTFLH DKNSREFLYY RRKVAEIRKE 250 260 270 280 290 300 AQKPQAATQK VSPPEDEEAK NLAEKLARFI ADGGPEVETI ALQNNRENQA FSFLYDPNSQ 310 320 330 340 350 360 GYRYYRQKLD EFRKAKAGST GSFPAPAPNP SLRRKSAPEA LSGAVPPITA CPTPVAPAPA 370 380 390 400 410 420 VNPTPSIPGK PTATAAVKRK RKSRWGPEED KVELPPAELA QRDIDASPSP LSVQDLKGLG 430 440 450 460 470 480 YEKGKPVGLV GVTELSDAQK KQLKEQQEMQ QMYDMIMQHK RAMQDMQLLW EKALQQHQHG 490 500 510 520 530 540 YDSDEEVDSE LGTWEHQLRR MEMDKTREWA EQLTQMGRGK HFIGDFLPPD ELEKFMETFK 550 560 570 580 590 600 ALKEGREPDY SEYKEFKLTV ENIGYQMLMK MGWKEGEGLG TEGQGIKNPV NKGATTIDGA 610 620 630 640 GFGIDRPAEL SKEDDEYEAF RKRMMLAYRF RPNPLNNPRR PYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11." Sampson N.D., Hewitt J.E. Gene 305:91-100(2003) [PubMed: 12594045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Spleen.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-483, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Solution structure of SURP domain in BAB30904." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 165-239.
[6]	"Solution structure of SURP domain in splicing factor 4." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 250-314.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF521129 mRNA. Translation: AAN77124.1.
AK156508 mRNA. Translation: BAE33738.1.
BC120919 mRNA. Translation: AAI20920.1.
BC120920 mRNA. Translation: AAI20921.1.

IPI

IPI00454015.

RefSeq

NP_081757.1. NM_027481.2.

UniGene

Mm.17665.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UG0	NMR	-	A	165-239	[»]
1X4O	NMR	-	A	250-314	[»]

ProteinModelPortal

Q8CH02.

SMR

Q8CH02. Positions 165-241, 250-319.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8CH02.

PTM databases

PhosphoSite

Q8CH02.

Proteomic databases

PRIDE

Q8CH02.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000011450; ENSMUSP00000011450; ENSMUSG00000011306.

GeneID

70616.

KEGG

mmu:70616.

UCSC

uc009lyn.1. mouse.

Organism-specific databases

CTD

57794.

MGI

MGI:1917866. Sugp1.

Phylogenomic databases

eggNOG

roNOG11907.

GeneTree

ENSGT00410000025695.

HOGENOM

HBG356498.

HOVERGEN

HBG079172.

InParanoid

Q8CH02.

OMA

EKVAMEN.

OrthoDB

EOG4VX24Z.

PhylomeDB

Q8CH02.

Gene expression databases

ArrayExpress

Q8CH02.

Bgee

Q8CH02.

CleanEx

MM_SF4.

Genevestigator

Q8CH02.

GermOnline

ENSMUSG00000011306. Mus musculus.

Family and domain databases

InterPro

IPR000467. G_patch.
IPR000061. Surp.
[Graphical view]

KO

K13096.

Pfam

PF01585. G-patch. 1 hit.
PF01805. Surp. 2 hits.
[Graphical view]

SMART

SM00443. G_patch. 1 hit.
SM00648. SWAP. 2 hits.
[Graphical view]

PROSITE

PS50174. G_PATCH. 1 hit.
PS50128. SURP. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

331984.

SOURCE

Search...

Entry information

Entry name

SUGP1_MOUSE

Accession

Primary (citable) accession number: Q8CH02
Secondary accession number(s): Q0VAT9, Q3U0W3, Q8R094

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 1, 2003
Last modified:	November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program