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Protein

Afadin- and alpha-actinin-binding protein

Gene

Ssx2ip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (PubMed:12446711). Involved in ciliogenesis (By similarity). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: RGD
  • protein domain specific binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Afadin- and alpha-actinin-binding protein
Short name:
ADIP
Alternative name(s):
Afadin DIL domain-interacting protein
Gene namesi
Name:Ssx2ip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi708490. Ssx2ip.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176790.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644571 – 613Afadin- and alpha-actinin-binding proteinAdd BLAST613

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei290PhosphoserineBy similarity1
Modified residuei293PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei537PhosphoserineBy similarity1
Modified residuei541PhosphoserineBy similarity1
Modified residuei543PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CGZ2.
PRIDEiQ8CGZ2.

PTM databases

iPTMnetiQ8CGZ2.
PhosphoSitePlusiQ8CGZ2.

Expressioni

Gene expression databases

BgeeiENSRNOG00000015425.

Interactioni

Subunit structurei

Interacts with SSX2 and SSX3 (By similarity). Does not interact with SSX1 and SSX4. Interacts with VAV2 (By similarity). Interacts with afadin and alpha-actinin (PubMed:12446711). Interacts with WRAP73 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

IntActiQ8CGZ2. 1 interactor.
STRINGi10116.ENSRNOP00000043877.

Structurei

3D structure databases

ProteinModelPortaliQ8CGZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili126 – 227Sequence analysisAdd BLAST102
Coiled coili266 – 293Sequence analysisAdd BLAST28
Coiled coili375 – 461Sequence analysisAdd BLAST87

Sequence similaritiesi

Belongs to the ADIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IED3. Eukaryota.
ENOG410XWZ9. LUCA.
GeneTreeiENSGT00390000007688.
HOGENOMiHOG000033818.
HOVERGENiHBG050460.
InParanoidiQ8CGZ2.
KOiK06085.
OMAiFLNMTTF.
OrthoDBiEOG091G0HRZ.
PhylomeDBiQ8CGZ2.
TreeFamiTF332889.

Family and domain databases

InterProiIPR021622. Afadin/alpha-actinin-bd.
[Graphical view]
PfamiPF11559. ADIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CGZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWMTVTDP VLCTENKNLS QYTSETKMSP SSLYSQQVLC SATPLSKNVH
60 70 80 90 100
GVFSAFCTGE NIEQSISYLD QELTTFGFPS LYEESKSKEA KRELSIVALL
110 120 130 140 150
NCMNELLVLQ RKNLLAQESV ETQNLKLGSD MDHLQSCYAK LKEQLEASRR
160 170 180 190 200
EMISLQERDR QLQCKNRNLH QLLKNEKEEV QKLQNIIASR ATQYNHDVKR
210 220 230 240 250
KEREYNKLKE RLHQLVMNKK DKNIAMDVLN YVGRVDGKRG SWRTDKTEAR
260 270 280 290 300
NEDEMYKILL NDYEYRQKQI LLENAELKKV LQQMKKEMIS LLSPQKKKPR
310 320 330 340 350
ERAEDSTGTV VISDVEDDAG ELSRDGVWSL SCDTVREQLT NSIRKQWRIL
360 370 380 390 400
KSHVEKLDNQ ASKVHSEGFH EEDVISRQDH EQETEKLELE IERCKEMIKA
410 420 430 440 450
QQQLLQQQLA TACDDDTTSL LRDCYLLEEK ERLKEEWSLF KEQKKNFERE
460 470 480 490 500
RRSFTEAAIR LGLERKAFEE ERASWVKQQF LNMTTFDHQN SENVKLFSAF
510 520 530 540 550
SGSSDPDNLI VHPRPRQKKP HSVANGVPAC TSKLAKSLPT SPSDFCPSRS
560 570 580 590 600
CVSEHSPVSA LTVTPEETKP NEVGRESTDQ KWSVVSRPSS REGCYGGCSS
610
AYTSSHVERD DLP
Length:613
Mass (Da):70,679
Last modified:March 1, 2003 - v1
Checksum:i55A6174A810AB8DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532970 mRNA. Translation: AAO15016.1.
BC078687 mRNA. Translation: AAH78687.1.
RefSeqiNP_783187.1. NM_175597.3.
XP_006233516.1. XM_006233454.3.
XP_006233517.1. XM_006233455.3.
UniGeneiRn.70527.

Genome annotation databases

EnsembliENSRNOT00000041186; ENSRNOP00000043877; ENSRNOG00000015425.
ENSRNOT00000084034; ENSRNOP00000072590; ENSRNOG00000015425.
GeneIDi308023.
KEGGirno:308023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532970 mRNA. Translation: AAO15016.1.
BC078687 mRNA. Translation: AAH78687.1.
RefSeqiNP_783187.1. NM_175597.3.
XP_006233516.1. XM_006233454.3.
XP_006233517.1. XM_006233455.3.
UniGeneiRn.70527.

3D structure databases

ProteinModelPortaliQ8CGZ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CGZ2. 1 interactor.
STRINGi10116.ENSRNOP00000043877.

Chemistry databases

ChEMBLiCHEMBL2176790.

PTM databases

iPTMnetiQ8CGZ2.
PhosphoSitePlusiQ8CGZ2.

Proteomic databases

PaxDbiQ8CGZ2.
PRIDEiQ8CGZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000041186; ENSRNOP00000043877; ENSRNOG00000015425.
ENSRNOT00000084034; ENSRNOP00000072590; ENSRNOG00000015425.
GeneIDi308023.
KEGGirno:308023.

Organism-specific databases

CTDi117178.
RGDi708490. Ssx2ip.

Phylogenomic databases

eggNOGiENOG410IED3. Eukaryota.
ENOG410XWZ9. LUCA.
GeneTreeiENSGT00390000007688.
HOGENOMiHOG000033818.
HOVERGENiHBG050460.
InParanoidiQ8CGZ2.
KOiK06085.
OMAiFLNMTTF.
OrthoDBiEOG091G0HRZ.
PhylomeDBiQ8CGZ2.
TreeFamiTF332889.

Miscellaneous databases

PROiQ8CGZ2.

Gene expression databases

BgeeiENSRNOG00000015425.

Family and domain databases

InterProiIPR021622. Afadin/alpha-actinin-bd.
[Graphical view]
PfamiPF11559. ADIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADIP_RAT
AccessioniPrimary (citable) accession number: Q8CGZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.