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Protein

Afadin- and alpha-actinin-binding protein

Gene

Ssx2ip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (PubMed:12446711). Involved in ciliogenesis (By similarity). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: RGD

GO - Biological processi

Keywordsi

Biological processCell adhesion, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Afadin- and alpha-actinin-binding protein
Short name:
ADIP
Alternative name(s):
Afadin DIL domain-interacting protein
Gene namesi
Name:Ssx2ip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi708490 Ssx2ip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176790

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644571 – 613Afadin- and alpha-actinin-binding proteinAdd BLAST613

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei290PhosphoserineBy similarity1
Modified residuei293PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei537PhosphoserineBy similarity1
Modified residuei541PhosphoserineBy similarity1
Modified residuei543PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CGZ2
PRIDEiQ8CGZ2

PTM databases

iPTMnetiQ8CGZ2
PhosphoSitePlusiQ8CGZ2

Expressioni

Gene expression databases

BgeeiENSRNOG00000015425
ExpressionAtlasiQ8CGZ2 baseline and differential

Interactioni

Subunit structurei

Interacts with SSX2 and SSX3 (By similarity). Does not interact with SSX1 and SSX4. Interacts with VAV2 (By similarity). Interacts with afadin and alpha-actinin (PubMed:12446711). Interacts with WRAP73 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: RGD

Protein-protein interaction databases

IntActiQ8CGZ2, 1 interactor
STRINGi10116.ENSRNOP00000043877

Structurei

3D structure databases

ProteinModelPortaliQ8CGZ2
SMRiQ8CGZ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili126 – 227Sequence analysisAdd BLAST102
Coiled coili266 – 293Sequence analysisAdd BLAST28
Coiled coili375 – 461Sequence analysisAdd BLAST87

Sequence similaritiesi

Belongs to the ADIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IED3 Eukaryota
ENOG410XWZ9 LUCA
GeneTreeiENSGT00390000007688
HOGENOMiHOG000033818
HOVERGENiHBG050460
InParanoidiQ8CGZ2
KOiK06085
OMAiNDYEYRQ
OrthoDBiEOG091G0HRZ
PhylomeDBiQ8CGZ2
TreeFamiTF332889

Family and domain databases

InterProiView protein in InterPro
IPR021622 Afadin/alpha-actinin-bd
PfamiView protein in Pfam
PF11559 ADIP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CGZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWMTVTDP VLCTENKNLS QYTSETKMSP SSLYSQQVLC SATPLSKNVH
60 70 80 90 100
GVFSAFCTGE NIEQSISYLD QELTTFGFPS LYEESKSKEA KRELSIVALL
110 120 130 140 150
NCMNELLVLQ RKNLLAQESV ETQNLKLGSD MDHLQSCYAK LKEQLEASRR
160 170 180 190 200
EMISLQERDR QLQCKNRNLH QLLKNEKEEV QKLQNIIASR ATQYNHDVKR
210 220 230 240 250
KEREYNKLKE RLHQLVMNKK DKNIAMDVLN YVGRVDGKRG SWRTDKTEAR
260 270 280 290 300
NEDEMYKILL NDYEYRQKQI LLENAELKKV LQQMKKEMIS LLSPQKKKPR
310 320 330 340 350
ERAEDSTGTV VISDVEDDAG ELSRDGVWSL SCDTVREQLT NSIRKQWRIL
360 370 380 390 400
KSHVEKLDNQ ASKVHSEGFH EEDVISRQDH EQETEKLELE IERCKEMIKA
410 420 430 440 450
QQQLLQQQLA TACDDDTTSL LRDCYLLEEK ERLKEEWSLF KEQKKNFERE
460 470 480 490 500
RRSFTEAAIR LGLERKAFEE ERASWVKQQF LNMTTFDHQN SENVKLFSAF
510 520 530 540 550
SGSSDPDNLI VHPRPRQKKP HSVANGVPAC TSKLAKSLPT SPSDFCPSRS
560 570 580 590 600
CVSEHSPVSA LTVTPEETKP NEVGRESTDQ KWSVVSRPSS REGCYGGCSS
610
AYTSSHVERD DLP
Length:613
Mass (Da):70,679
Last modified:March 1, 2003 - v1
Checksum:i55A6174A810AB8DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532970 mRNA Translation: AAO15016.1
BC078687 mRNA Translation: AAH78687.1
RefSeqiNP_783187.1, NM_175597.3
XP_006233516.1, XM_006233454.3
XP_006233517.1, XM_006233455.3
UniGeneiRn.70527

Genome annotation databases

EnsembliENSRNOT00000041186; ENSRNOP00000043877; ENSRNOG00000015425
ENSRNOT00000084034; ENSRNOP00000072590; ENSRNOG00000015425
GeneIDi308023
KEGGirno:308023

Similar proteinsi

Entry informationi

Entry nameiADIP_RAT
AccessioniPrimary (citable) accession number: Q8CGZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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