Reviewed, UniProtKB/Swiss-Prot Q8CGZ0 (CHERP_MOUSE)

Names and origin

Protein names

Recommended name:
    Calcium homeostasis endoplasmic reticulum protein
Alternative name(s):
    SR-related CTD-associated factor 6

Gene names

Name:	Cherp
Synonyms:	Scaf6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	936 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in calcium homeostasis, growth and proliferation By similarity. UniProtKB Q8IWX8
Subcellular location	Cytoplasm By similarity. Cytoplasm › perinuclear region By similarity. Endoplasmic reticulum By similarity. Note: Distributed throughout the cytoplasm and also localizes to the perinuclear region. Colocalizes with ITPR1 By similarity. UniProtKB Q8IWX8
Sequence similarities	Contains 1 CID domain. Contains 1 G-patch domain. Contains 1 SURP motif repeat.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.3 (identifier: Q8CGZ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: Q8CGZ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: C → WALTRGRSLPHS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 936	936	Calcium homeostasis endoplasmic reticulum protein		PRO_0000299493
Regions
	Repeat	15 – 57	43	SURP motif
	Domain	149 – 289	141	CID
	Domain	850 – 900	51	G-patch
	Compositional bias	279 – 355	77	Gln-rich
	Compositional bias	343 – 691	349	Pro-rich
	Compositional bias	727 – 825	99	Arg-rich
Amino acid modifications
	Modified residue	723	1	Phosphotyrosine Ref.3
	Modified residue	822	1	Phosphoserine Ref.4
	Modified residue	824	1	Phosphoserine By similarity
	Modified residue	826	1	Phosphoserine
	Modified residue	828	1	Phosphothreonine
	Modified residue	831	1	Phosphoserine By similarity
	Modified residue	837	1	Phosphoserine By similarity
	Modified residue	839	1	Phosphoserine By similarity
	Modified residue	913	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	225	1	C → WALTRGRSLPHS in isoform 2. Ref.2		VSP_052525
Experimental info
	Sequence conflict	348 – 349	2	Missing in AAH20488. Ref.2
	Sequence conflict	348 – 349	2	Missing in AAH32191. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8C29DF0822CA9EB3	FASTA	936	106,169
	10 20 30 40 50 60 MEMPMPPDDQ ELRNVIDKLA QFVARNGPEF EKMTMEKQKD NPKFSFLFGG EFYSYYKCKL 70 80 90 100 110 120 ALEQQQLICK QQAPELEPTS AMPPLPQPPL APTASLTPAQ GTPSMDELIQ QSQWSLQQQE 130 140 150 160 170 180 QHLLALRQEQ VTTAVAHAVE QQMQKLLEET QLDMSEFDNL LQPIIDTCTK DAISAGKNWM 190 200 210 220 230 240 FSNAKSPPHC ELMAGHLRNR ITADGAHFEL RLHLIYLIND VLHHCQRKQA RELLAALQKV 250 260 270 280 290 300 VVPIYCTSFL AVEEDKQQKI ARLLQLWEKN GYFDDSIIQQ LQSPALGLGQ YQATLINEYS 310 320 330 340 350 360 SVVQPVQLAF QQQIQSLKTQ HEEFVSSLAQ QQQQQQQQQQ QQPQPQPQPQ IQLPQMEADV 370 380 390 400 410 420 KATPPPPAPP PASAPAPTIP PTTQPDDNKP PIQMPGSSEY DTSAGVQDPA AAGPRGPGPH 430 440 450 460 470 480 EQIPPNKPPW FDQPHPVAPW GQQQPPEQPP YPHHQGGPPH CPPWNNSHEG MWGEQRGDPG 490 500 510 520 530 540 WNGQRDAPWN NQPDPNWNNQ FEGPWNNQHE PPPWGGAQRE PPFRMQRPPH FRGPFPPHQQ 550 560 570 580 590 600 HPQFNQPPHP HNFNRFPPRF MQDDFPPRHP FERPPYPHRF DYPQGDFPAD MGPPHHHPGH 610 620 630 640 650 660 RMPHPGINEH PPWAGPQHPD FGPPPHGFNG QPPHMRRQGP PHINHDDPSL VPNVPYFDLP 670 680 690 700 710 720 AGLMAPLVKL EDHEYKPLDP KDIRLPPPMP PSERLLAAVE AFYSPPSHDR PRNSEGWEQN 730 740 750 760 770 780 GLYEFFRAKM RARRRKGQEK RNSGPSRSRS RSKSRGRSSS RSSSRSSKSS RSSSRSHSRS 790 800 810 820 830 840 RSRSSSRSRS RSRSRSRSSR SRSRSRSRSR SKSYSPGRRR RSRSRSPTPP SAAGLGSNSA 850 860 870 880 890 900 PPIPDSRLGE ENKGHQMLVK MGWSGSGGLG AKEQGIQDPI KGGDVRDKWD QYKGVGVALD 910 920 930 DPYENYRRNK SYSFIARMKA RDEFSTFGTR KEEKED
	Isoform 2. Checksum: D76311CCF38EE732	FASTA	947	107,428
	10 20 30 40 50 60 MEMPMPPDDQ ELRNVIDKLA QFVARNGPEF EKMTMEKQKD NPKFSFLFGG EFYSYYKCKL 70 80 90 100 110 120 ALEQQQLICK QQAPELEPTS AMPPLPQPPL APTASLTPAQ GTPSMDELIQ QSQWSLQQQE 130 140 150 160 170 180 QHLLALRQEQ VTTAVAHAVE QQMQKLLEET QLDMSEFDNL LQPIIDTCTK DAISAGKNWM 190 200 210 220 230 240 FSNAKSPPHC ELMAGHLRNR ITADGAHFEL RLHLIYLIND VLHHWALTRG RSLPHSQRKQ 250 260 270 280 290 300 ARELLAALQK VVVPIYCTSF LAVEEDKQQK IARLLQLWEK NGYFDDSIIQ QLQSPALGLG 310 320 330 340 350 360 QYQATLINEY SSVVQPVQLA FQQQIQSLKT QHEEFVSSLA QQQQQQQQQQ QQQPQPQPQP 370 380 390 400 410 420 QIQLPQMEAD VKATPPPPAP PPASAPAPTI PPTTQPDDNK PPIQMPGSSE YDTSAGVQDP 430 440 450 460 470 480 AAAGPRGPGP HEQIPPNKPP WFDQPHPVAP WGQQQPPEQP PYPHHQGGPP HCPPWNNSHE 490 500 510 520 530 540 GMWGEQRGDP GWNGQRDAPW NNQPDPNWNN QFEGPWNNQH EPPPWGGAQR EPPFRMQRPP 550 560 570 580 590 600 HFRGPFPPHQ QHPQFNQPPH PHNFNRFPPR FMQDDFPPRH PFERPPYPHR FDYPQGDFPA 610 620 630 640 650 660 DMGPPHHHPG HRMPHPGINE HPPWAGPQHP DFGPPPHGFN GQPPHMRRQG PPHINHDDPS 670 680 690 700 710 720 LVPNVPYFDL PAGLMAPLVK LEDHEYKPLD PKDIRLPPPM PPSERLLAAV EAFYSPPSHD 730 740 750 760 770 780 RPRNSEGWEQ NGLYEFFRAK MRARRRKGQE KRNSGPSRSR SRSKSRGRSS SRSSSRSSKS 790 800 810 820 830 840 SRSSSRSHSR SRSRSSSRSR SRSRSRSRSS RSRSRSRSRS RSKSYSPGRR RRSRSRSPTP 850 860 870 880 890 900 PSAAGLGSNS APPIPDSRLG EENKGHQMLV KMGWSGSGGL GAKEQGIQDP IKGGDVRDKW 910 920 930 940 DQYKGVGVAL DDPYENYRRN KSYSFIARMK ARDEFSTFGT RKEEKED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional characterization of the novel SR-related CTD associated factor, SCAF6." Sampson N.D., Hewitt J.E. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: FVB/N. Tissue: Colon and Mammary tumor.
[3]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-723, MASS SPECTROMETRY. Tissue: Mast cell.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-822, MASS SPECTROMETRY. Tissue: Liver.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-826 AND THR-828, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF536541 mRNA. Translation: AAN77182.1. BC020488 mRNA. Translation: AAH20488.2. BC032191 mRNA. Translation: AAH32191.1. Different initiation.
IPI	IPI00229604. IPI00858053.
RefSeq	NP_613051.3.
UniGene	Mm.30136
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8CGZ0.
Proteomic databases
PRIDE	Q8CGZ0.
Genome annotation databases
Ensembl	ENSMUSG00000052488. Mus musculus. [Contig view]
GeneID	27967.
KEGG	mmu:27967.
Organism-specific databases
MGI	MGI:106417. Cherp.
Phylogenomic databases
HOGENOM	Q8CGZ0.
HOVERGEN	Q8CGZ0.
Gene expression databases
Bgee	Q8CGZ0.
CleanEx	MM_CHERP.
Family and domain databases
InterPro	IPR006903. DUF618. IPR000467. G_patch. IPR006569. RPR. IPR000061. Surp. [Graphical view]
Pfam	PF04818. DUF618. 1 hit. PF01585. G-patch. 1 hit. PF01805. Surp. 1 hit. [Graphical view]
SMART	SM00443. G_patch. 1 hit. SM00582. RPR. 1 hit. SM00648. SWAP. 1 hit. [Graphical view]
PROSITE	PS51391. CID. 1 hit. PS50174. G_PATCH. 1 hit. PS50128. SURP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	306460.
SOURCE	Search...

Entry information

Entry name

CHERP_MOUSE

Accession

Primary (citable) accession number: Q8CGZ0
Secondary accession number(s): Q8K291, Q8VCD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)