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Protein

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit

Gene

Ogt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (By similarity). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2.By similarity2 Publications

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine.By similarity
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei508Proton acceptorBy similarity1
Binding sitei849UDPBy similarity1
Binding sitei852UDPBy similarity1
Binding sitei935UDPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi905 – 908UDPBy similarity4
Nucleotide bindingi911 – 914UDPBy similarity4
Nucleotide bindingi929 – 931UDPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Glycosyltransferase, Transferase

Keywords - Biological processi

Biological rhythms

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BRENDAi2.4.1.255. 3474.
ReactomeiR-MMU-3214847. HATs acetylate histones.
R-MMU-5689603. UCH proteinases.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT41. Glycosyltransferase Family 41.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (EC:2.4.1.255By similarity)
Alternative name(s):
O-GlcNAc transferase subunit p110
O-linked N-acetylglucosamine transferase 110 kDa subunit
Short name:
OGT
Gene namesi
Name:Ogt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1339639. Ogt.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Cell membrane By similarity

  • Note: Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • histone acetyltransferase complex Source: UniProtKB
  • intracellular Source: MGI
  • MLL5-L complex Source: MGI
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002852162 – 1046UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunitAdd BLAST1045

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei3Phosphoserine; by GSK3-beta; alternate1 Publication1
Glycosylationi3O-linked (GlcNAc); alternate1 Publication1
Modified residuei4Phosphoserine; by GSK3-beta; alternate1 Publication1
Glycosylationi4O-linked (GlcNAc); alternate1 Publication1
Modified residuei20PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated, leading to its proteasomal degradation.By similarity
Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8CGY8.
PeptideAtlasiQ8CGY8.
PRIDEiQ8CGY8.

PTM databases

iPTMnetiQ8CGY8.
PhosphoSitePlusiQ8CGY8.

Expressioni

Inductioni

Expression in the liver oscillates in a circadian manner.1 Publication

Gene expression databases

BgeeiENSMUSG00000034160.
GenevisibleiQ8CGY8. MM.

Interactioni

Subunit structurei

Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7 (By similarity). Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, HCFC1, PPP1CC and ACTB. Component of a THAP1/THAP3-HCFC1-OGT complex. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts directly with HCFC1; the interaction O-glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus. Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase (By similarity). Interacts (via TPR 5-6) with TET1, TET2 and TET3. Interacts (via TPR repeats 6 and 7) with ATXN10. Interacts with ARNTL/BMAL1.By similarity3 Publications

Protein-protein interaction databases

BioGridi223870. 16 interactors.
DIPiDIP-35700N.
IntActiQ8CGY8. 7 interactors.
MINTiMINT-5097933.
STRINGi10090.ENSMUSP00000045409.

Structurei

3D structure databases

ProteinModelPortaliQ8CGY8.
SMRiQ8CGY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 54TPR 1Add BLAST34
Repeati89 – 122TPR 2Add BLAST34
Repeati123 – 156TPR 3Add BLAST34
Repeati157 – 190TPR 4Add BLAST34
Repeati191 – 224TPR 5Add BLAST34
Repeati225 – 258TPR 6Add BLAST34
Repeati259 – 292TPR 7Add BLAST34
Repeati293 – 326TPR 8Add BLAST34
Repeati327 – 360TPR 9Add BLAST34
Repeati361 – 394TPR 10Add BLAST34
Repeati395 – 428TPR 11Add BLAST34
Repeati429 – 462TPR 12Add BLAST34
Repeati463 – 473TPR 13; truncatedAdd BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni991 – 1010Required for phosphatidylinositol 3,4,5-triphosphate bindingAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi487 – 503Nuclear localization signalSequence analysisAdd BLAST17

Domaini

The TPR repeat domain is required for substrate binding and oligomerization.By similarity

Sequence similaritiesi

Contains 13 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
KOG4626. Eukaryota.
COG3914. LUCA.
GeneTreeiENSGT00550000074327.
HOGENOMiHOG000003765.
HOVERGENiHBG000351.
InParanoidiQ8CGY8.
KOiK09667.
OMAiKLAYMPN.
OrthoDBiEOG091G024Y.
PhylomeDBiQ8CGY8.
TreeFamiTF105785.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR029489. OGT/SEC/SPY_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13844. Glyco_transf_41. 1 hit.
PF00515. TPR_1. 2 hits.
PF13414. TPR_11. 3 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 12 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 5 hits.
PROSITEiPS50005. TPR. 12 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CGY8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSVGNVAD STEPTKRMLS FQGLAELAHR EYQAGDFEAA ERHCMQLWRQ
60 70 80 90 100
EPDNTGVLLL LSSIHFQCRR LDRSAHFSTL AIKQNPLLAE AYSNLGNVYK
110 120 130 140 150
ERGQLQEAIE HYRHALRLKP DFIDGYINLA AALVAAGDME GAVQAYVSAL
160 170 180 190 200
QYNPDLYCVR SDLGNLLKAL GRLEEAKACY LKAIETQPNF AVAWSNLGCV
210 220 230 240 250
FNAQGEIWLA IHHFEKAVTL DPNFLDAYIN LGNVLKEARI FDRAVAAYLR
260 270 280 290 300
ALSLSPNHAV VHGNLACVYY EQGLIDLAID TYRRAIELQP HFPDAYCNLA
310 320 330 340 350
NALKEKGSVA EAEDCYNTAL RLCPTHADSL NNLANIKREQ GNIEEAVRLY
360 370 380 390 400
RKALEVFPEF AAAHSNLASV LQQQGKLQEA LMHYKEAIRI SPTFADAYSN
410 420 430 440 450
MGNTLKEMQD VQGALQCYTR AIQINPAFAD AHSNLASIHK DSGNIPEAIA
460 470 480 490 500
SYRTALKLKP DFPDAYCNLA HCLQIVCDWT DYDERMKKLV SIVAEQLEKN
510 520 530 540 550
RLPSVHPHHS MLYPLSHGFR KAIAERHGNL CLDKINVLHK PPYEHPKDLK
560 570 580 590 600
LSDGRLRVGY VSSDFGNHPT SHLMQSIPGM HNPDKFEVFC YALSPDDGTN
610 620 630 640 650
FRVKVMAEAN HFIDLSQIPC NGKAADRIHQ DGIHILVNMN GYTKGARNEL
660 670 680 690 700
FALRPAPIQA MWLGYPGTSG ALFMDYIITD QETSPAEVAE QYSEKLAYMP
710 720 730 740 750
HTFFIGDHAN MFPHLKKKAV IDFKSNGHIY DNRIVLNGID LKAFLDSLPD
760 770 780 790 800
VKIVKMKCPD GGDNPDSSNT ALNMPVIPMN TIAEAVIEMI NRGQIQITIN
810 820 830 840 850
GFSISNGLAT TQINNKAATG EEVPRTIIVT TRSQYGLPED AIVYCNFNQL
860 870 880 890 900
YKIDPSTLQM WANILKRVPN SVLWLLRFPA VGEPNIQQYA QNMGLPQNRI
910 920 930 940 950
IFSPVAPKEE HVRRGQLADV CLDTPLCNGH TTGMDVLWAG TPMVTMPGET
960 970 980 990 1000
LASRVAASQL TCLGCLELIA KSRQEYEDIA VKLGTDLEYL KKIRGKVWKQ
1010 1020 1030 1040
RISSPLFNTK QYTMELERLY LQMWEHYAAG NKPDHMIKPV EVTESA
Length:1,046
Mass (Da):116,952
Last modified:May 1, 2007 - v2
Checksum:iFD5EE12844E00097
GO
Isoform 2 (identifier: Q8CGY8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     13-22: Missing.

Show »
Length:1,036
Mass (Da):115,733
Checksum:iBBCD509790239136
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57V → A in AAO17363 (PubMed:12504895).Curated1
Sequence conflicti992K → R in AAK39123 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02484413 – 22Missing in isoform 2. Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539527 Genomic DNA. Translation: AAO17363.1.
AF363030 mRNA. Translation: AAK39123.1.
AL806534, AL805980 Genomic DNA. Translation: CAM21281.1.
AL806534, AL805980 Genomic DNA. Translation: CAM21282.1.
AL805980, AL806534 Genomic DNA. Translation: CAM25748.1.
AL805980, AL806534 Genomic DNA. Translation: CAM25749.1.
BC057319 mRNA. Translation: AAH57319.1.
AK047095 mRNA. Translation: BAC32961.1.
CCDSiCCDS30318.1. [Q8CGY8-1]
CCDS72415.1. [Q8CGY8-2]
RefSeqiNP_001277464.1. NM_001290535.1. [Q8CGY8-2]
NP_631883.2. NM_139144.4. [Q8CGY8-1]
XP_006527799.1. XM_006527736.3. [Q8CGY8-1]
XP_011245805.1. XM_011247503.2. [Q8CGY8-2]
UniGeneiMm.487332.
Mm.491168.

Genome annotation databases

EnsembliENSMUST00000044475; ENSMUSP00000045409; ENSMUSG00000034160. [Q8CGY8-1]
ENSMUST00000119299; ENSMUSP00000113454; ENSMUSG00000034160. [Q8CGY8-2]
GeneIDi108155.
KEGGimmu:108155.
UCSCiuc009tyc.3. mouse. [Q8CGY8-1]
uc057ask.1. mouse. [Q8CGY8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539527 Genomic DNA. Translation: AAO17363.1.
AF363030 mRNA. Translation: AAK39123.1.
AL806534, AL805980 Genomic DNA. Translation: CAM21281.1.
AL806534, AL805980 Genomic DNA. Translation: CAM21282.1.
AL805980, AL806534 Genomic DNA. Translation: CAM25748.1.
AL805980, AL806534 Genomic DNA. Translation: CAM25749.1.
BC057319 mRNA. Translation: AAH57319.1.
AK047095 mRNA. Translation: BAC32961.1.
CCDSiCCDS30318.1. [Q8CGY8-1]
CCDS72415.1. [Q8CGY8-2]
RefSeqiNP_001277464.1. NM_001290535.1. [Q8CGY8-2]
NP_631883.2. NM_139144.4. [Q8CGY8-1]
XP_006527799.1. XM_006527736.3. [Q8CGY8-1]
XP_011245805.1. XM_011247503.2. [Q8CGY8-2]
UniGeneiMm.487332.
Mm.491168.

3D structure databases

ProteinModelPortaliQ8CGY8.
SMRiQ8CGY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223870. 16 interactors.
DIPiDIP-35700N.
IntActiQ8CGY8. 7 interactors.
MINTiMINT-5097933.
STRINGi10090.ENSMUSP00000045409.

Protein family/group databases

CAZyiGT41. Glycosyltransferase Family 41.

PTM databases

iPTMnetiQ8CGY8.
PhosphoSitePlusiQ8CGY8.

Proteomic databases

PaxDbiQ8CGY8.
PeptideAtlasiQ8CGY8.
PRIDEiQ8CGY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044475; ENSMUSP00000045409; ENSMUSG00000034160. [Q8CGY8-1]
ENSMUST00000119299; ENSMUSP00000113454; ENSMUSG00000034160. [Q8CGY8-2]
GeneIDi108155.
KEGGimmu:108155.
UCSCiuc009tyc.3. mouse. [Q8CGY8-1]
uc057ask.1. mouse. [Q8CGY8-2]

Organism-specific databases

CTDi8473.
MGIiMGI:1339639. Ogt.

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
KOG4626. Eukaryota.
COG3914. LUCA.
GeneTreeiENSGT00550000074327.
HOGENOMiHOG000003765.
HOVERGENiHBG000351.
InParanoidiQ8CGY8.
KOiK09667.
OMAiKLAYMPN.
OrthoDBiEOG091G024Y.
PhylomeDBiQ8CGY8.
TreeFamiTF105785.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.255. 3474.
ReactomeiR-MMU-3214847. HATs acetylate histones.
R-MMU-5689603. UCH proteinases.

Miscellaneous databases

ChiTaRSiOgt. mouse.
PROiQ8CGY8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034160.
GenevisibleiQ8CGY8. MM.

Family and domain databases

Gene3Di1.25.40.10. 5 hits.
InterProiIPR029489. OGT/SEC/SPY_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13844. Glyco_transf_41. 1 hit.
PF00515. TPR_1. 2 hits.
PF13414. TPR_11. 3 hits.
PF13181. TPR_8. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 12 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 5 hits.
PROSITEiPS50005. TPR. 12 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOGT1_MOUSE
AccessioniPrimary (citable) accession number: Q8CGY8
Secondary accession number(s): A2ALY1
, Q6PG10, Q8BXH6, Q91Y38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.