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Protein

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit

Gene

Ogt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Probably by glycosylating KMT2E, stabilizes KMT2E by preventing its ubiquitination (By similarity).Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues. O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (By similarity). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:23337503, PubMed:23395176). O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (By similarity).By similarity2 Publications

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine.By similarity
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei508Proton acceptorBy similarity1
Binding sitei849UDPBy similarity1
Binding sitei852UDPBy similarity1
Binding sitei935UDPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi905 – 908UDPBy similarity4
Nucleotide bindingi911 – 914UDPBy similarity4
Nucleotide bindingi929 – 931UDPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Glycosyltransferase, Transferase
Biological processBiological rhythms
LigandLipid-binding

Enzyme and pathway databases

BRENDAi2.4.1.255 3474
ReactomeiR-MMU-3214847 HATs acetylate histones
R-MMU-5689603 UCH proteinases
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT41 Glycosyltransferase Family 41

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (EC:2.4.1.255By similarity)
Alternative name(s):
O-GlcNAc transferase subunit p110
O-linked N-acetylglucosamine transferase 110 kDa subunit
Short name:
OGT
Gene namesi
Name:Ogt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1339639 Ogt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002852162 – 1046UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunitAdd BLAST1045

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei3Phosphoserine; by GSK3-beta; alternate1 Publication1
Glycosylationi3O-linked (GlcNAc) serine; alternate1 Publication1
Modified residuei4Phosphoserine; by GSK3-beta; alternate1 Publication1
Glycosylationi4O-linked (GlcNAc) serine; alternate1 Publication1
Modified residuei20PhosphoserineCombined sources1
Modified residuei989PhosphotyrosineBy similarity1

Post-translational modificationi

Ubiquitinated, leading to its proteasomal degradation.By similarity
Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.1 Publication

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8CGY8
PeptideAtlasiQ8CGY8
PRIDEiQ8CGY8

PTM databases

iPTMnetiQ8CGY8
PhosphoSitePlusiQ8CGY8

Expressioni

Inductioni

Expression in the liver oscillates in a circadian manner.1 Publication

Gene expression databases

BgeeiENSMUSG00000034160
GenevisibleiQ8CGY8 MM

Interactioni

Subunit structurei

Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7 (By similarity). Component of a THAP1/THAP3-HCFC1-OGT complex (By similarity). Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1 (By similarity). Interacts directly with HCFC1; the interaction O-glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus (By similarity). Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase (By similarity). Interacts (via TPR 5-6) with TET1, TET2 and TET3 (PubMed:23352454). Interacts (via TPR repeats 6 and 7) with ATXN10 (PubMed:16182253). Interacts with histone H2B (By similarity). Interacts with ARNTL/BMAL1 (PubMed:23337503). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1 (PubMed:28554894). Interacts with SINHCAF (PubMed:28554894). Component of a complex composed of KMT2E, OGT and USP7; the complex stabilizes KMT2E, preventing KMT2E ubiquitination and proteosomal-mediated degradation (By similarity). Interacts (via TRP repeats) with KMT2E (via N-terminus) (By similarity). Interacts with USP7 (By similarity).By similarity4 Publications

Protein-protein interaction databases

BioGridi223870, 16 interactors
DIPiDIP-35700N
IntActiQ8CGY8, 10 interactors
MINTiQ8CGY8
STRINGi10090.ENSMUSP00000045409

Structurei

3D structure databases

ProteinModelPortaliQ8CGY8
SMRiQ8CGY8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati21 – 54TPR 1Add BLAST34
Repeati89 – 122TPR 2Add BLAST34
Repeati123 – 156TPR 3Add BLAST34
Repeati157 – 190TPR 4Add BLAST34
Repeati191 – 224TPR 5Add BLAST34
Repeati225 – 258TPR 6Add BLAST34
Repeati259 – 292TPR 7Add BLAST34
Repeati293 – 326TPR 8Add BLAST34
Repeati327 – 360TPR 9Add BLAST34
Repeati361 – 394TPR 10Add BLAST34
Repeati395 – 428TPR 11Add BLAST34
Repeati429 – 462TPR 12Add BLAST34
Repeati463 – 473TPR 13; truncatedAdd BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni991 – 1010Required for phosphatidylinositol 3,4,5-triphosphate bindingAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi487 – 503Nuclear localization signalSequence analysisAdd BLAST17

Domaini

The TPR repeat domain is required for substrate binding and oligomerization.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124 Eukaryota
KOG4626 Eukaryota
COG3914 LUCA
GeneTreeiENSGT00550000074327
HOGENOMiHOG000003765
HOVERGENiHBG000351
InParanoidiQ8CGY8
KOiK09667
OMAiPFMDYII
OrthoDBiEOG091G024Y
PhylomeDBiQ8CGY8
TreeFamiTF105785

Family and domain databases

Gene3Di1.25.40.10, 2 hits
InterProiView protein in InterPro
IPR037919 OGT
IPR029489 OGT/SEC/SPY_C
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PANTHERiPTHR44366 PTHR44366, 1 hit
PfamiView protein in Pfam
PF13844 Glyco_transf_41, 1 hit
PF00515 TPR_1, 2 hits
PF13181 TPR_8, 2 hits
SMARTiView protein in SMART
SM00028 TPR, 12 hits
SUPFAMiSSF48452 SSF48452, 5 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 12 hits
PS50293 TPR_REGION, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CGY8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSVGNVAD STEPTKRMLS FQGLAELAHR EYQAGDFEAA ERHCMQLWRQ
60 70 80 90 100
EPDNTGVLLL LSSIHFQCRR LDRSAHFSTL AIKQNPLLAE AYSNLGNVYK
110 120 130 140 150
ERGQLQEAIE HYRHALRLKP DFIDGYINLA AALVAAGDME GAVQAYVSAL
160 170 180 190 200
QYNPDLYCVR SDLGNLLKAL GRLEEAKACY LKAIETQPNF AVAWSNLGCV
210 220 230 240 250
FNAQGEIWLA IHHFEKAVTL DPNFLDAYIN LGNVLKEARI FDRAVAAYLR
260 270 280 290 300
ALSLSPNHAV VHGNLACVYY EQGLIDLAID TYRRAIELQP HFPDAYCNLA
310 320 330 340 350
NALKEKGSVA EAEDCYNTAL RLCPTHADSL NNLANIKREQ GNIEEAVRLY
360 370 380 390 400
RKALEVFPEF AAAHSNLASV LQQQGKLQEA LMHYKEAIRI SPTFADAYSN
410 420 430 440 450
MGNTLKEMQD VQGALQCYTR AIQINPAFAD AHSNLASIHK DSGNIPEAIA
460 470 480 490 500
SYRTALKLKP DFPDAYCNLA HCLQIVCDWT DYDERMKKLV SIVAEQLEKN
510 520 530 540 550
RLPSVHPHHS MLYPLSHGFR KAIAERHGNL CLDKINVLHK PPYEHPKDLK
560 570 580 590 600
LSDGRLRVGY VSSDFGNHPT SHLMQSIPGM HNPDKFEVFC YALSPDDGTN
610 620 630 640 650
FRVKVMAEAN HFIDLSQIPC NGKAADRIHQ DGIHILVNMN GYTKGARNEL
660 670 680 690 700
FALRPAPIQA MWLGYPGTSG ALFMDYIITD QETSPAEVAE QYSEKLAYMP
710 720 730 740 750
HTFFIGDHAN MFPHLKKKAV IDFKSNGHIY DNRIVLNGID LKAFLDSLPD
760 770 780 790 800
VKIVKMKCPD GGDNPDSSNT ALNMPVIPMN TIAEAVIEMI NRGQIQITIN
810 820 830 840 850
GFSISNGLAT TQINNKAATG EEVPRTIIVT TRSQYGLPED AIVYCNFNQL
860 870 880 890 900
YKIDPSTLQM WANILKRVPN SVLWLLRFPA VGEPNIQQYA QNMGLPQNRI
910 920 930 940 950
IFSPVAPKEE HVRRGQLADV CLDTPLCNGH TTGMDVLWAG TPMVTMPGET
960 970 980 990 1000
LASRVAASQL TCLGCLELIA KSRQEYEDIA VKLGTDLEYL KKIRGKVWKQ
1010 1020 1030 1040
RISSPLFNTK QYTMELERLY LQMWEHYAAG NKPDHMIKPV EVTESA
Length:1,046
Mass (Da):116,952
Last modified:May 1, 2007 - v2
Checksum:iFD5EE12844E00097
GO
Isoform 2 (identifier: Q8CGY8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     13-22: Missing.

Show »
Length:1,036
Mass (Da):115,733
Checksum:iBBCD509790239136
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57V → A in AAO17363 (PubMed:12504895).Curated1
Sequence conflicti992K → R in AAK39123 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02484413 – 22Missing in isoform 2. Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539527 Genomic DNA Translation: AAO17363.1
AF363030 mRNA Translation: AAK39123.1
AL806534, AL805980 Genomic DNA Translation: CAM21281.1
AL806534, AL805980 Genomic DNA Translation: CAM21282.1
AL805980, AL806534 Genomic DNA Translation: CAM25748.1
AL805980, AL806534 Genomic DNA Translation: CAM25749.1
BC057319 mRNA Translation: AAH57319.1
AK047095 mRNA Translation: BAC32961.1
CCDSiCCDS30318.1 [Q8CGY8-1]
CCDS72415.1 [Q8CGY8-2]
RefSeqiNP_001277464.1, NM_001290535.1 [Q8CGY8-2]
NP_631883.2, NM_139144.4 [Q8CGY8-1]
XP_006527799.1, XM_006527736.3 [Q8CGY8-1]
XP_011245805.1, XM_011247503.2 [Q8CGY8-2]
UniGeneiMm.487332
Mm.491168

Genome annotation databases

EnsembliENSMUST00000044475; ENSMUSP00000045409; ENSMUSG00000034160 [Q8CGY8-1]
ENSMUST00000119299; ENSMUSP00000113454; ENSMUSG00000034160 [Q8CGY8-2]
GeneIDi108155
KEGGimmu:108155
UCSCiuc009tyc.3 mouse [Q8CGY8-1]
uc057ask.1 mouse [Q8CGY8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiOGT1_MOUSE
AccessioniPrimary (citable) accession number: Q8CGY8
Secondary accession number(s): A2ALY1
, Q6PG10, Q8BXH6, Q91Y38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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