Q8CGY8 (OGT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
EC=2.4.1.-

Alternative name(s):
O-GlcNAc transferase subunit p110
O-linked N-acetylglucosamine transferase 110 kDa subunit

Gene names

Name:

Ogt

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1046 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1 By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Heterotrimer of two 110 kDa and one 70 kDa subunits. It is not known if the 70 kDa subunit is encoded by a separate gene or is the product of either of a proteolytic degradation or an alternative initiation of the 110 kDa subunit By similarity. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts directly with HCFC1 By similarity. Interacts with ATXN10. Ref.7
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Mostly in the nucleus By similarity.
Domain	The TPR repeat domain mediates recognition of protein substrates By similarity.
Post-translational modification	Ubiquitinated, leading to its proteasomal degradation By similarity.
Sequence similarities	Belongs to the O-GlcNAc transferase family. Contains 13 TPR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat TPR repeat
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of proteolysis Inferred from sequence or structural similarity. Source: UniProtKB protein O-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	N-acetyltransferase activity Traceable author statement. Source: MGI protein N-acetylglucosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CGY8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CGY8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     13-22: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1046	1045	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit		PRO_0000285216
Regions
	Repeat	21 – 54	34	TPR 1
	Repeat	89 – 122	34	TPR 2
	Repeat	123 – 156	34	TPR 3
	Repeat	157 – 190	34	TPR 4
	Repeat	191 – 224	34	TPR 5
	Repeat	225 – 258	34	TPR 6
	Repeat	259 – 292	34	TPR 7
	Repeat	293 – 326	34	TPR 8
	Repeat	327 – 360	34	TPR 9
	Repeat	361 – 394	34	TPR 10
	Repeat	395 – 428	34	TPR 11
	Repeat	429 – 462	34	TPR 12
	Repeat	463 – 473	11	TPR 13; truncated
	Nucleotide binding	905 – 908	4	UDP By similarity
	Nucleotide binding	911 – 914	4	UDP By similarity
	Nucleotide binding	929 – 931	3	UDP By similarity
	Motif	487 – 503	17	Nuclear localization signal Potential
Sites
	Active site	508	1	Proton acceptor By similarity
	Binding site	849	1	UDP By similarity
	Binding site	852	1	UDP By similarity
	Binding site	935	1	UDP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	20	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	13 – 22	10	Missing in isoform 2.		VSP_024844
Experimental info
	Sequence conflict	57	1	V → A in AAO17363. Ref.1
	Sequence conflict	992	1	K → R in AAK39123. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: FD5EE12844E00097	FASTA	1,046	116,952
	10 20 30 40 50 60 MASSVGNVAD STEPTKRMLS FQGLAELAHR EYQAGDFEAA ERHCMQLWRQ EPDNTGVLLL 70 80 90 100 110 120 LSSIHFQCRR LDRSAHFSTL AIKQNPLLAE AYSNLGNVYK ERGQLQEAIE HYRHALRLKP 130 140 150 160 170 180 DFIDGYINLA AALVAAGDME GAVQAYVSAL QYNPDLYCVR SDLGNLLKAL GRLEEAKACY 190 200 210 220 230 240 LKAIETQPNF AVAWSNLGCV FNAQGEIWLA IHHFEKAVTL DPNFLDAYIN LGNVLKEARI 250 260 270 280 290 300 FDRAVAAYLR ALSLSPNHAV VHGNLACVYY EQGLIDLAID TYRRAIELQP HFPDAYCNLA 310 320 330 340 350 360 NALKEKGSVA EAEDCYNTAL RLCPTHADSL NNLANIKREQ GNIEEAVRLY RKALEVFPEF 370 380 390 400 410 420 AAAHSNLASV LQQQGKLQEA LMHYKEAIRI SPTFADAYSN MGNTLKEMQD VQGALQCYTR 430 440 450 460 470 480 AIQINPAFAD AHSNLASIHK DSGNIPEAIA SYRTALKLKP DFPDAYCNLA HCLQIVCDWT 490 500 510 520 530 540 DYDERMKKLV SIVAEQLEKN RLPSVHPHHS MLYPLSHGFR KAIAERHGNL CLDKINVLHK 550 560 570 580 590 600 PPYEHPKDLK LSDGRLRVGY VSSDFGNHPT SHLMQSIPGM HNPDKFEVFC YALSPDDGTN 610 620 630 640 650 660 FRVKVMAEAN HFIDLSQIPC NGKAADRIHQ DGIHILVNMN GYTKGARNEL FALRPAPIQA 670 680 690 700 710 720 MWLGYPGTSG ALFMDYIITD QETSPAEVAE QYSEKLAYMP HTFFIGDHAN MFPHLKKKAV 730 740 750 760 770 780 IDFKSNGHIY DNRIVLNGID LKAFLDSLPD VKIVKMKCPD GGDNPDSSNT ALNMPVIPMN 790 800 810 820 830 840 TIAEAVIEMI NRGQIQITIN GFSISNGLAT TQINNKAATG EEVPRTIIVT TRSQYGLPED 850 860 870 880 890 900 AIVYCNFNQL YKIDPSTLQM WANILKRVPN SVLWLLRFPA VGEPNIQQYA QNMGLPQNRI 910 920 930 940 950 960 IFSPVAPKEE HVRRGQLADV CLDTPLCNGH TTGMDVLWAG TPMVTMPGET LASRVAASQL 970 980 990 1000 1010 1020 TCLGCLELIA KSRQEYEDIA VKLGTDLEYL KKIRGKVWKQ RISSPLFNTK QYTMELERLY 1030 1040 LQMWEHYAAG NKPDHMIKPV EVTESA
	Isoform 2 [UniParc]. Checksum: BBCD509790239136	FASTA	1,036	115,733
	10 20 30 40 50 60 MASSVGNVAD STGLAELAHR EYQAGDFEAA ERHCMQLWRQ EPDNTGVLLL LSSIHFQCRR 70 80 90 100 110 120 LDRSAHFSTL AIKQNPLLAE AYSNLGNVYK ERGQLQEAIE HYRHALRLKP DFIDGYINLA 130 140 150 160 170 180 AALVAAGDME GAVQAYVSAL QYNPDLYCVR SDLGNLLKAL GRLEEAKACY LKAIETQPNF 190 200 210 220 230 240 AVAWSNLGCV FNAQGEIWLA IHHFEKAVTL DPNFLDAYIN LGNVLKEARI FDRAVAAYLR 250 260 270 280 290 300 ALSLSPNHAV VHGNLACVYY EQGLIDLAID TYRRAIELQP HFPDAYCNLA NALKEKGSVA 310 320 330 340 350 360 EAEDCYNTAL RLCPTHADSL NNLANIKREQ GNIEEAVRLY RKALEVFPEF AAAHSNLASV 370 380 390 400 410 420 LQQQGKLQEA LMHYKEAIRI SPTFADAYSN MGNTLKEMQD VQGALQCYTR AIQINPAFAD 430 440 450 460 470 480 AHSNLASIHK DSGNIPEAIA SYRTALKLKP DFPDAYCNLA HCLQIVCDWT DYDERMKKLV 490 500 510 520 530 540 SIVAEQLEKN RLPSVHPHHS MLYPLSHGFR KAIAERHGNL CLDKINVLHK PPYEHPKDLK 550 560 570 580 590 600 LSDGRLRVGY VSSDFGNHPT SHLMQSIPGM HNPDKFEVFC YALSPDDGTN FRVKVMAEAN 610 620 630 640 650 660 HFIDLSQIPC NGKAADRIHQ DGIHILVNMN GYTKGARNEL FALRPAPIQA MWLGYPGTSG 670 680 690 700 710 720 ALFMDYIITD QETSPAEVAE QYSEKLAYMP HTFFIGDHAN MFPHLKKKAV IDFKSNGHIY 730 740 750 760 770 780 DNRIVLNGID LKAFLDSLPD VKIVKMKCPD GGDNPDSSNT ALNMPVIPMN TIAEAVIEMI 790 800 810 820 830 840 NRGQIQITIN GFSISNGLAT TQINNKAATG EEVPRTIIVT TRSQYGLPED AIVYCNFNQL 850 860 870 880 890 900 YKIDPSTLQM WANILKRVPN SVLWLLRFPA VGEPNIQQYA QNMGLPQNRI IFSPVAPKEE 910 920 930 940 950 960 HVRRGQLADV CLDTPLCNGH TTGMDVLWAG TPMVTMPGET LASRVAASQL TCLGCLELIA 970 980 990 1000 1010 1020 KSRQEYEDIA VKLGTDLEYL KKIRGKVWKQ RISSPLFNTK QYTMELERLY LQMWEHYAAG 1030 NKPDHMIKPV EVTESA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial and nucleocytoplasmic isoforms of O-linked GlcNAc transferase encoded by a single mammalian gene." Hanover J.A., Yu S., Lubas W.B., Shin S.H., Ragano-Caracciola M., Kochran J., Love D.C. Arch. Biochem. Biophys. 409:287-297(2003) [PubMed: 12504895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6.
[2]	"Molecular cloning of the mouse O-GlcNAc transferase." Rumberger J.M., Wu T., Hering M.A., Marshall S. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c. Tissue: Skeletal muscle.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6 and FVB/N. Tissue: Brain and Mammary tumor.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 894-1046 (ISOFORMS 1/2). Strain: C57BL/6J. Tissue: Cerebellum and Head.
[6]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 217-236 AND 421-440, MASS SPECTROMETRY. Tissue: Hippocampus.
[7]	"Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic beta cells." Andrali S.S., Maerz P., Ozcan S. Biochem. Biophys. Res. Commun. 337:149-153(2005) [PubMed: 16182253] [Abstract] Cited for: INTERACTION WITH ATXN10.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF539527 Genomic DNA. Translation: AAO17363.1. AF363030 mRNA. Translation: AAK39123.1. AL806534, AL805980 Genomic DNA. Translation: CAM21281.1. AL806534, AL805980 Genomic DNA. Translation: CAM21282.1. AL805980, AL806534 Genomic DNA. Translation: CAM25748.1. AL805980, AL806534 Genomic DNA. Translation: CAM25749.1. BC057319 mRNA. Translation: AAH57319.1. AK047095 mRNA. Translation: BAC32961.1.
IPI	IPI00420870. IPI00845528.
RefSeq	NP_631883.2. NM_139144.3.
UniGene	Mm.485463.
3D structure databases
HSSP	HSSP built from PDB template 1W3B based on UniProtKB O15294.
ProteinModelPortal	Q8CGY8.
SMR	Q8CGY8. Positions 23-1038.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CGY8. 3 interactions.
STRING	Q8CGY8.
Protein family/group databases
CAZy	GT41. Glycosyltransferase Family 41.
Proteomic databases
PRIDE	Q8CGY8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000044475; ENSMUSP00000045409; ENSMUSG00000034160. ENSMUST00000119299; ENSMUSP00000113454; ENSMUSG00000034160.
GeneID	108155.
KEGG	mmu:108155.
NMPDR	fig|10090.3.peg.21960.
UCSC	uc009tyc.2. mouse. uc009tyd.2. mouse.
Organism-specific databases
CTD	8473.
MGI	MGI:1339639. Ogt.
Phylogenomic databases
eggNOG	roNOG07233.
GeneTree	ENSGT00550000074327.
HOGENOM	HBG317910.
HOVERGEN	HBG000351.
InParanoid	Q8CGY8.
OMA	NFPDAYC.
OrthoDB	EOG4HQDHJ.
PhylomeDB	Q8CGY8.
Gene expression databases
ArrayExpress	Q8CGY8.
Bgee	Q8CGY8.
Genevestigator	Q8CGY8.
Family and domain databases
InterPro	IPR001440. TPR-1. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 2 hits.
KO	K09667.
Pfam	PF00515. TPR_1. 10 hits. [Graphical view]
SMART	SM00028. TPR. 12 hits. [Graphical view]
PROSITE	PS50005. TPR. 12 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	360182.
SOURCE	Search...

Entry information

Entry name

OGT1_MOUSE

Accession

Primary (citable) accession number: Q8CGY8
Secondary accession number(s): A2ALY1 , Q6PG10, Q8BXH6, Q91Y38

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	November 16, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program