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Q8CGV9

- TSH3_MOUSE

UniProt

Q8CGV9 - TSH3_MOUSE

Protein

Teashirt homolog 3

Gene

Tshz3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri214 – 23825C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri275 – 29925C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri387 – 41024C2H2-type 3; atypicalPROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi891 – 96171Homeobox; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri976 – 99823C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1041 – 106424C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding Source: InterPro
    5. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. kidney morphogenesis Source: MGI
    3. kidney smooth muscle cell differentiation Source: UniProtKB
    4. lung development Source: UniProtKB
    5. metanephros development Source: UniProtKB
    6. musculoskeletal movement Source: MGI
    7. negative regulation of transcription, DNA-templated Source: UniProtKB
    8. positive regulation of smooth muscle cell differentiation Source: MGI
    9. regulation of respiratory gaseous exchange by neurological system process Source: UniProtKB
    10. sensory perception of touch Source: MGI
    11. smooth muscle tissue development Source: MGI
    12. transcription, DNA-templated Source: UniProtKB-KW
    13. ureteric bud development Source: MGI
    14. ureteric peristalsis Source: UniProtKB
    15. ureter smooth muscle cell differentiation Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Teashirt homolog 3
    Alternative name(s):
    Zinc finger protein 537
    Gene namesi
    Name:Tshz3
    Synonyms:Kiaa1474, Tsh3, Zfp537, Znf537
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2442819. Tshz3.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation. Cell projectiongrowth cone By similarity
    Note: Colocalizes with APBB1 in the nucleus. Colocalizes with APBB1 in axonal growth cone By similarity.By similarity

    GO - Cellular componenti

    1. growth cone Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice fail to breathe and die at birth. Display pronounced cell death of motoneurons in the nucleus ambiguus and induce strong alterations of rhythmogenesis in the embryonic parafacial respiratory group (e-pF or pFRG) oscillator and cranial motoneurons that control the upper airways. Mice also fail to form small smooth cells in the proximal ureter and urine flow is impaired because of functional obstruction caused by absent peristalsis in the proximal ureter that leads to hydronephrosis and hydroureter phenotypes.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10811081Teashirt homolog 3PRO_0000047067Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei682 – 6821PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ8CGV9.

    PTM databases

    PhosphoSiteiQ8CGV9.

    Expressioni

    Developmental stagei

    Expressed in branchio and viscero motoneurons at 10.5 dpc. Expressed in the nucleus ambiguus (nA) motoneurons at 15.5 dpc. Expressed in the brainstem respiratory rhythm generator (RRG), including two interacting neuronal networks constituting two oscillators: the pre-Boetzinger complex (preBoetC) at 15.5 dpc and the embryonic parafacial respiratory group (e-pF or pFRG) at 14.4 dpc, that contributes both to motor coordination of the respiratory apparatus and confers central chemosensitivity, as well as in cranial motoneurons targeting chest muscles that control the upper airway opening. Expressed in the developing urinary tract. Expressed in ureteric bud (UB) stalk at 11.5 dpc. Expressed in mesenchymal cells along and around the UB stalk, and absent from the UB epithelium and in scattered cells within the metanephric medullary stroma at 12.5 dpc. Expressed in mesenchymal cells in proximal ureters at 14 dpc, preceding the smooth muscle precursor cells differentiation and the expression of contractile proteins from 15 dpc. Expressed in mesenchymal cells of the ureter and renal pelvis, and in renal medullary stroma, in the mesenchymal cells adjacent to the epithelium and in the peripheral mesenchyme where smooth muscle (SM) starts to differentiate; the outer rim of nephrogenic mesenchyme was negative at 15.5. In the bladder, expressed in the submucosal loose connective tissue adjacent to the epithelium and in the detrusor SM layer at 18.5 dpc.3 Publications

    Inductioni

    Up-regulated by BMP4.

    Gene expression databases

    ArrayExpressiQ8CGV9.
    BgeeiQ8CGV9.
    CleanExiMM_TSHZ3.
    GenevestigatoriQ8CGV9.

    Interactioni

    Subunit structurei

    Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 By similarity. Interacts (via homeobox domain) with APBB1 (via PID domain 1).By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CGV9.
    SMRiQ8CGV9. Positions 200-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili606 – 63025Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi142 – 16423Ser-richAdd
    BLAST
    Compositional biasi493 – 4964Poly-Glu

    Sequence similaritiesi

    Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri214 – 23825C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri275 – 29925C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri387 – 41024C2H2-type 3; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri976 – 99823C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri1041 – 106424C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Homeobox, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG83709.
    GeneTreeiENSGT00390000014977.
    HOGENOMiHOG000231480.
    HOVERGENiHBG079626.
    InParanoidiQ8CGV9.
    KOiK09236.
    OrthoDBiEOG7NPFSF.
    PhylomeDBiQ8CGV9.
    TreeFamiTF328447.

    Family and domain databases

    InterProiIPR001356. Homeobox_dom.
    IPR027008. Teashirt_fam.
    IPR026810. Tshz3.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PANTHERiPTHR12487. PTHR12487. 1 hit.
    PTHR12487:SF5. PTHR12487:SF5. 1 hit.
    SMARTiSM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 5 hits.
    [Graphical view]
    PROSITEiPS50071. HOMEOBOX_2. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CGV9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRRKQQAPR RAAAYVSDEL KAAALVEDDV EPEEQAADGE PSAKYMCPEK     50
    ELSKACPSYQ NSPAAEFSSH EMDSESHISE TSDRMADFES SSIKNEEETK 100
    EVQVPLEDTT VSDSLEQMKA VYNNFLSNSY WSNLNLNLHQ PSSENNGGSS 150
    SSSSSSSSSC GSGSFDWHQS AMAKTLQQVS QNRMLPEPSL FSTVQLYRQS 200
    SKLYGSIFTG ASKFRCKDCS AAYDTLVELT VHMNETGHYR DDNHETDNNN 250
    PKRWSKPRKR SLLEMEGKED AQKVLKCMYC GHSFESLQDL SVHMIKTKHY 300
    QKVPLKEPVT PVAAKIIPAA RKKPSLELEL PSSPDSTGGT PKATLSDASD 350
    ALQKNSNPYI TPNNRYGHQN GASYAWHFEA RKSQILKCME CGSSHDTLQE 400
    LTAHMMVTGH FIKVTNSAMK KGKPIMETPV TPTITTLLDE KVQSVPLAAT 450
    TFTSPSNTPA SVSPKLAVEI KKEVDKEKAV PDEKPKEREK PSEEEEKYDI 500
    SSKYHYLTEN DLEESPKGGL DILKSLENTV TSAINKAQNG TPSWGGYPSI 550
    HAAYQLPNMM KLSLGSSGKS TPLKPMFGNS EIVSPTKTQT LVSPPSSQTS 600
    PMPKTNFHAM EELVKKVTEK VAKVEEKMKE PEGKLSPPKR ATPSPCSSEQ 650
    SEPIKMEASS DGSFKSQENS PSPPRDACKE ASPSAEPVEN GKELVKPLSG 700
    GLSGSTAIIT DHPPEQPFVN PLSALQSVMN IHLGKAAKPS LPALDPMSML 750
    FKMSNSLAEK AAVATPPPLQ AKKAEHLDRY FYHVNNDQPI DLTKGKSDKG 800
    CSLGSGLLSP TSTSPATSSS TVTTAKTSAV VSFMSNSPLR ENALSDISDM 850
    LKNLTESHTS KSSTPSSISE KSDIDGATLE EAEESTPAQK RKGRQSNWNP 900
    QHLLILQAQF AASLRQTSEG KYIMSDLSPQ ERMHISRFTG LSMTTISHWL 950
    ANVKYQLRRT GGTKFLKNLD TGHPVFFCND CASQIRTPST YISHLESHLG 1000
    FRLRDLSKLS TEQINNQIAQ TKSPSEKLVT SSPEEDLGTT YQCKLCNRTF 1050
    ASKHAVKLHL SKTHGKSPED HLLFVSELEK Q 1081
    Length:1,081
    Mass (Da):118,626
    Last modified:January 10, 2006 - v2
    Checksum:iC69AD8A9A2816669
    GO

    Sequence cautioni

    The sequence BAD90255.1 differs from that shown. Reason: Frameshift at position 78.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1101T → I in AAL38978. (PubMed:14973285)Curated
    Sequence conflicti663 – 6631S → G in BAD90255. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY063491 mRNA. Translation: AAL38978.1.
    AK220396 mRNA. Translation: BAD90255.1. Frameshift.
    CCDSiCCDS39913.1.
    RefSeqiNP_758502.1. NM_172298.2.
    UniGeneiMm.44141.

    Genome annotation databases

    EnsembliENSMUST00000021641; ENSMUSP00000021641; ENSMUSG00000021217.
    GeneIDi243931.
    KEGGimmu:243931.
    UCSCiuc009gkj.1. mouse.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Life's first breath - Issue 122 of October 2010

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY063491 mRNA. Translation: AAL38978.1 .
    AK220396 mRNA. Translation: BAD90255.1 . Frameshift.
    CCDSi CCDS39913.1.
    RefSeqi NP_758502.1. NM_172298.2.
    UniGenei Mm.44141.

    3D structure databases

    ProteinModelPortali Q8CGV9.
    SMRi Q8CGV9. Positions 200-303.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8CGV9.

    Proteomic databases

    PRIDEi Q8CGV9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021641 ; ENSMUSP00000021641 ; ENSMUSG00000021217 .
    GeneIDi 243931.
    KEGGi mmu:243931.
    UCSCi uc009gkj.1. mouse.

    Organism-specific databases

    CTDi 57616.
    MGIi MGI:2442819. Tshz3.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG83709.
    GeneTreei ENSGT00390000014977.
    HOGENOMi HOG000231480.
    HOVERGENi HBG079626.
    InParanoidi Q8CGV9.
    KOi K09236.
    OrthoDBi EOG7NPFSF.
    PhylomeDBi Q8CGV9.
    TreeFami TF328447.

    Miscellaneous databases

    NextBioi 386042.
    PROi Q8CGV9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CGV9.
    Bgeei Q8CGV9.
    CleanExi MM_TSHZ3.
    Genevestigatori Q8CGV9.

    Family and domain databases

    InterProi IPR001356. Homeobox_dom.
    IPR027008. Teashirt_fam.
    IPR026810. Tshz3.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    PANTHERi PTHR12487. PTHR12487. 1 hit.
    PTHR12487:SF5. PTHR12487:SF5. 1 hit.
    SMARTi SM00389. HOX. 1 hit.
    SM00355. ZnF_C2H2. 5 hits.
    [Graphical view ]
    PROSITEi PS50071. HOMEOBOX_2. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Three putative murine Teashirt orthologues specify trunk structures in Drosophila in the same way as the Drosophila teashirt gene."
      Manfroid I., Caubit X., Kerridge S., Fasano L.
      Development 131:1065-1073(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Fetal brain.
    3. "Teashirt 3 is necessary for ureteral smooth muscle differentiation downstream of SHH and BMP4."
      Caubit X., Lye C.M., Martin E., Core N., Long D.A., Vola C., Jenkins D., Garratt A.N., Skaer H., Woolf A.S., Fasano L.
      Development 135:3301-3310(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    4. Cited for: INTERACTION WITH APBB1.
    5. "Teashirt 3 regulates development of neurons involved in both respiratory rhythm and airflow control."
      Caubit X., Thoby-Brisson M., Voituron N., Filippi P., Bevengut M., Faralli H., Zanella S., Fortin G., Hilaire G., Fasano L.
      J. Neurosci. 30:9465-9476(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    6. "Analysis of TSHZ2 and TSHZ3 genes in congenital pelvi-ureteric junction obstruction."
      Jenkins D., Caubit X., Dimovski A., Matevska N., Lye C.M., Cabuk F., Gucev Z., Tasic V., Fasano L., Woolf A.S.
      Nephrol. Dial. Transplant. 25:54-60(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiTSH3_MOUSE
    AccessioniPrimary (citable) accession number: Q8CGV9
    Secondary accession number(s): Q5DTX4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3