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Q8CGV9

- TSH3_MOUSE

UniProt

Q8CGV9 - TSH3_MOUSE

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Protein

Teashirt homolog 3

Gene

Tshz3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri214 – 23825C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri275 – 29925C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri387 – 41024C2H2-type 3; atypicalPROSITE-ProRule annotationAdd
BLAST
DNA bindingi891 – 96171Homeobox; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri976 – 99823C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1041 – 106424C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: UniProtKB
  2. DNA binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. in utero embryonic development Source: MGI
  2. kidney morphogenesis Source: MGI
  3. kidney smooth muscle cell differentiation Source: UniProtKB
  4. lung development Source: UniProtKB
  5. metanephros development Source: UniProtKB
  6. musculoskeletal movement Source: MGI
  7. negative regulation of transcription, DNA-templated Source: UniProtKB
  8. positive regulation of smooth muscle cell differentiation Source: MGI
  9. regulation of respiratory gaseous exchange by neurological system process Source: UniProtKB
  10. sensory perception of touch Source: MGI
  11. smooth muscle tissue development Source: MGI
  12. transcription, DNA-templated Source: UniProtKB-KW
  13. ureteric bud development Source: MGI
  14. ureteric peristalsis Source: UniProtKB
  15. ureter smooth muscle cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Teashirt homolog 3
Alternative name(s):
Zinc finger protein 537
Gene namesi
Name:Tshz3
Synonyms:Kiaa1474, Tsh3, Zfp537, Znf537
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2442819. Tshz3.

Subcellular locationi

Nucleus PROSITE-ProRule annotation. Cell projectiongrowth cone By similarity
Note: Colocalizes with APBB1 in the nucleus. Colocalizes with APBB1 in axonal growth cone (By similarity).By similarity

GO - Cellular componenti

  1. growth cone Source: Ensembl
  2. mitochondrion Source: MGI
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice fail to breathe and die at birth. Display pronounced cell death of motoneurons in the nucleus ambiguus and induce strong alterations of rhythmogenesis in the embryonic parafacial respiratory group (e-pF or pFRG) oscillator and cranial motoneurons that control the upper airways. Mice also fail to form small smooth cells in the proximal ureter and urine flow is impaired because of functional obstruction caused by absent peristalsis in the proximal ureter that leads to hydronephrosis and hydroureter phenotypes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10811081Teashirt homolog 3PRO_0000047067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei682 – 6821PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CGV9.
PRIDEiQ8CGV9.

PTM databases

PhosphoSiteiQ8CGV9.

Expressioni

Developmental stagei

Expressed in branchio and viscero motoneurons at 10.5 dpc. Expressed in the nucleus ambiguus (nA) motoneurons at 15.5 dpc. Expressed in the brainstem respiratory rhythm generator (RRG), including two interacting neuronal networks constituting two oscillators: the pre-Boetzinger complex (preBoetC) at 15.5 dpc and the embryonic parafacial respiratory group (e-pF or pFRG) at 14.4 dpc, that contributes both to motor coordination of the respiratory apparatus and confers central chemosensitivity, as well as in cranial motoneurons targeting chest muscles that control the upper airway opening. Expressed in the developing urinary tract. Expressed in ureteric bud (UB) stalk at 11.5 dpc. Expressed in mesenchymal cells along and around the UB stalk, and absent from the UB epithelium and in scattered cells within the metanephric medullary stroma at 12.5 dpc. Expressed in mesenchymal cells in proximal ureters at 14 dpc, preceding the smooth muscle precursor cells differentiation and the expression of contractile proteins from 15 dpc. Expressed in mesenchymal cells of the ureter and renal pelvis, and in renal medullary stroma, in the mesenchymal cells adjacent to the epithelium and in the peripheral mesenchyme where smooth muscle (SM) starts to differentiate; the outer rim of nephrogenic mesenchyme was negative at 15.5. In the bladder, expressed in the submucosal loose connective tissue adjacent to the epithelium and in the detrusor SM layer at 18.5 dpc.3 Publications

Inductioni

Up-regulated by BMP4.

Gene expression databases

BgeeiQ8CGV9.
CleanExiMM_TSHZ3.
ExpressionAtlasiQ8CGV9. baseline and differential.
GenevestigatoriQ8CGV9.

Interactioni

Subunit structurei

Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 (By similarity). Interacts (via homeobox domain) with APBB1 (via PID domain 1).By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8CGV9.
SMRiQ8CGV9. Positions 200-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili606 – 63025Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi142 – 16423Ser-richAdd
BLAST
Compositional biasi493 – 4964Poly-Glu

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri214 – 23825C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri275 – 29925C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri387 – 41024C2H2-type 3; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri976 – 99823C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1041 – 106424C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG83709.
GeneTreeiENSGT00390000014977.
HOGENOMiHOG000231480.
HOVERGENiHBG079626.
InParanoidiQ8CGV9.
KOiK09236.
OrthoDBiEOG7NPFSF.
PhylomeDBiQ8CGV9.
TreeFamiTF328447.

Family and domain databases

InterProiIPR001356. Homeobox_dom.
IPR027008. Teashirt_fam.
IPR026810. Tshz3.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERiPTHR12487. PTHR12487. 1 hit.
PTHR12487:SF5. PTHR12487:SF5. 1 hit.
SMARTiSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CGV9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRRKQQAPR RAAAYVSDEL KAAALVEDDV EPEEQAADGE PSAKYMCPEK
60 70 80 90 100
ELSKACPSYQ NSPAAEFSSH EMDSESHISE TSDRMADFES SSIKNEEETK
110 120 130 140 150
EVQVPLEDTT VSDSLEQMKA VYNNFLSNSY WSNLNLNLHQ PSSENNGGSS
160 170 180 190 200
SSSSSSSSSC GSGSFDWHQS AMAKTLQQVS QNRMLPEPSL FSTVQLYRQS
210 220 230 240 250
SKLYGSIFTG ASKFRCKDCS AAYDTLVELT VHMNETGHYR DDNHETDNNN
260 270 280 290 300
PKRWSKPRKR SLLEMEGKED AQKVLKCMYC GHSFESLQDL SVHMIKTKHY
310 320 330 340 350
QKVPLKEPVT PVAAKIIPAA RKKPSLELEL PSSPDSTGGT PKATLSDASD
360 370 380 390 400
ALQKNSNPYI TPNNRYGHQN GASYAWHFEA RKSQILKCME CGSSHDTLQE
410 420 430 440 450
LTAHMMVTGH FIKVTNSAMK KGKPIMETPV TPTITTLLDE KVQSVPLAAT
460 470 480 490 500
TFTSPSNTPA SVSPKLAVEI KKEVDKEKAV PDEKPKEREK PSEEEEKYDI
510 520 530 540 550
SSKYHYLTEN DLEESPKGGL DILKSLENTV TSAINKAQNG TPSWGGYPSI
560 570 580 590 600
HAAYQLPNMM KLSLGSSGKS TPLKPMFGNS EIVSPTKTQT LVSPPSSQTS
610 620 630 640 650
PMPKTNFHAM EELVKKVTEK VAKVEEKMKE PEGKLSPPKR ATPSPCSSEQ
660 670 680 690 700
SEPIKMEASS DGSFKSQENS PSPPRDACKE ASPSAEPVEN GKELVKPLSG
710 720 730 740 750
GLSGSTAIIT DHPPEQPFVN PLSALQSVMN IHLGKAAKPS LPALDPMSML
760 770 780 790 800
FKMSNSLAEK AAVATPPPLQ AKKAEHLDRY FYHVNNDQPI DLTKGKSDKG
810 820 830 840 850
CSLGSGLLSP TSTSPATSSS TVTTAKTSAV VSFMSNSPLR ENALSDISDM
860 870 880 890 900
LKNLTESHTS KSSTPSSISE KSDIDGATLE EAEESTPAQK RKGRQSNWNP
910 920 930 940 950
QHLLILQAQF AASLRQTSEG KYIMSDLSPQ ERMHISRFTG LSMTTISHWL
960 970 980 990 1000
ANVKYQLRRT GGTKFLKNLD TGHPVFFCND CASQIRTPST YISHLESHLG
1010 1020 1030 1040 1050
FRLRDLSKLS TEQINNQIAQ TKSPSEKLVT SSPEEDLGTT YQCKLCNRTF
1060 1070 1080
ASKHAVKLHL SKTHGKSPED HLLFVSELEK Q
Length:1,081
Mass (Da):118,626
Last modified:January 10, 2006 - v2
Checksum:iC69AD8A9A2816669
GO

Sequence cautioni

The sequence BAD90255.1 differs from that shown. Reason: Frameshift at position 78.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101T → I in AAL38978. (PubMed:14973285)Curated
Sequence conflicti663 – 6631S → G in BAD90255. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY063491 mRNA. Translation: AAL38978.1.
AK220396 mRNA. Translation: BAD90255.1. Frameshift.
CCDSiCCDS39913.1.
RefSeqiNP_758502.1. NM_172298.2.
UniGeneiMm.44141.

Genome annotation databases

EnsembliENSMUST00000021641; ENSMUSP00000021641; ENSMUSG00000021217.
GeneIDi243931.
KEGGimmu:243931.
UCSCiuc009gkj.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Life's first breath - Issue 122 of October 2010

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY063491 mRNA. Translation: AAL38978.1 .
AK220396 mRNA. Translation: BAD90255.1 . Frameshift.
CCDSi CCDS39913.1.
RefSeqi NP_758502.1. NM_172298.2.
UniGenei Mm.44141.

3D structure databases

ProteinModelPortali Q8CGV9.
SMRi Q8CGV9. Positions 200-303.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8CGV9.

Proteomic databases

MaxQBi Q8CGV9.
PRIDEi Q8CGV9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021641 ; ENSMUSP00000021641 ; ENSMUSG00000021217 .
GeneIDi 243931.
KEGGi mmu:243931.
UCSCi uc009gkj.1. mouse.

Organism-specific databases

CTDi 57616.
MGIi MGI:2442819. Tshz3.
Rougei Search...

Phylogenomic databases

eggNOGi NOG83709.
GeneTreei ENSGT00390000014977.
HOGENOMi HOG000231480.
HOVERGENi HBG079626.
InParanoidi Q8CGV9.
KOi K09236.
OrthoDBi EOG7NPFSF.
PhylomeDBi Q8CGV9.
TreeFami TF328447.

Miscellaneous databases

NextBioi 386042.
PROi Q8CGV9.
SOURCEi Search...

Gene expression databases

Bgeei Q8CGV9.
CleanExi MM_TSHZ3.
ExpressionAtlasi Q8CGV9. baseline and differential.
Genevestigatori Q8CGV9.

Family and domain databases

InterProi IPR001356. Homeobox_dom.
IPR027008. Teashirt_fam.
IPR026810. Tshz3.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
PANTHERi PTHR12487. PTHR12487. 1 hit.
PTHR12487:SF5. PTHR12487:SF5. 1 hit.
SMARTi SM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view ]
PROSITEi PS50071. HOMEOBOX_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Three putative murine Teashirt orthologues specify trunk structures in Drosophila in the same way as the Drosophila teashirt gene."
    Manfroid I., Caubit X., Kerridge S., Fasano L.
    Development 131:1065-1073(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal brain.
  3. "Teashirt 3 is necessary for ureteral smooth muscle differentiation downstream of SHH and BMP4."
    Caubit X., Lye C.M., Martin E., Core N., Long D.A., Vola C., Jenkins D., Garratt A.N., Skaer H., Woolf A.S., Fasano L.
    Development 135:3301-3310(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  4. Cited for: INTERACTION WITH APBB1.
  5. "Teashirt 3 regulates development of neurons involved in both respiratory rhythm and airflow control."
    Caubit X., Thoby-Brisson M., Voituron N., Filippi P., Bevengut M., Faralli H., Zanella S., Fortin G., Hilaire G., Fasano L.
    J. Neurosci. 30:9465-9476(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
  6. "Analysis of TSHZ2 and TSHZ3 genes in congenital pelvi-ureteric junction obstruction."
    Jenkins D., Caubit X., Dimovski A., Matevska N., Lye C.M., Cabuk F., Gucev Z., Tasic V., Fasano L., Woolf A.S.
    Nephrol. Dial. Transplant. 25:54-60(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiTSH3_MOUSE
AccessioniPrimary (citable) accession number: Q8CGV9
Secondary accession number(s): Q5DTX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: October 29, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3