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Q8CGV9 (TSH3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Teashirt homolog 3
Alternative name(s):
Zinc finger protein 537
Gene names
Name:Tshz3
Synonyms:Kiaa1474, Tsh3, Zfp537, Znf537
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1081 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter. Ref.1 Ref.3 Ref.5 Ref.6

Subunit structure

Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 By similarity. Interacts (via homeobox domain) with APBB1 (via PID domain 1). Ref.4

Subcellular location

Nucleus By similarity. Cell projectiongrowth cone By similarity. Note: Colocalizes with APBB1 in the nucleus. Colocalizes with APBB1 in axonal growth cone By similarity.

Developmental stage

Expressed in branchio and viscero motoneurons at 10.5 dpc. Expressed in the nucleus ambiguus (nA) motoneurons at 15.5 dpc. Expressed in the brainstem respiratory rhythm generator (RRG), including two interacting neuronal networks constituting two oscillators: the pre-Boetzinger complex (preBoetC) at 15.5 dpc and the embryonic parafacial respiratory group (e-pF or pFRG) at 14.4 dpc, that contributes both to motor coordination of the respiratory apparatus and confers central chemosensitivity, as well as in cranial motoneurons targeting chest muscles that control the upper airway opening. Expressed in the developing urinary tract. Expressed in ureteric bud (UB) stalk at 11.5 dpc. Expressed in mesenchymal cells along and around the UB stalk, and absent from the UB epithelium and in scattered cells within the metanephric medullary stroma at 12.5 dpc. Expressed in mesenchymal cells in proximal ureters at 14 dpc, preceding the smooth muscle precursor cells differentiation and the expression of contractile proteins from 15 dpc. Expressed in mesenchymal cells of the ureter and renal pelvis, and in renal medullary stroma, in the mesenchymal cells adjacent to the epithelium and in the peripheral mesenchyme where smooth muscle (SM) starts to differentiate; the outer rim of nephrogenic mesenchyme was negative at 15.5. In the bladder, expressed in the submucosal loose connective tissue adjacent to the epithelium and in the detrusor SM layer at 18.5 dpc. Ref.3 Ref.5 Ref.6

Induction

Up-regulated by BMP4.

Disruption phenotype

Mice fail to breathe and die at birth. Display pronounced cell death of motoneurons in the nucleus ambiguus and induce strong alterations of rhythmogenesis in the embryonic parafacial respiratory group (e-pF or pFRG) oscillator and cranial motoneurons that control the upper airways. Mice also fail to form small smooth cells in the proximal ureter and urine flow is impaired because of functional obstruction caused by absent peristalsis in the proximal ureter that leads to hydronephrosis and hydroureter phenotypes. Ref.3 Ref.5

Sequence similarities

Belongs to the teashirt C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Contains 1 homeobox DNA-binding domain.

Sequence caution

The sequence BAD90255.1 differs from that shown. Reason: Frameshift at position 78.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCell projection
Nucleus
   DomainCoiled coil
Homeobox
Repeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionDevelopmental protein
Repressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processin utero embryonic development

Inferred from mutant phenotype Ref.3. Source: MGI

kidney morphogenesis

Inferred from mutant phenotype Ref.3. Source: MGI

kidney smooth muscle cell differentiation

Inferred from mutant phenotype Ref.3. Source: UniProtKB

lung development

Inferred from mutant phenotype Ref.3. Source: UniProtKB

metanephros development

Inferred from expression pattern Ref.3. Source: UniProtKB

musculoskeletal movement

Inferred from mutant phenotype Ref.3. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of smooth muscle cell differentiation

Inferred from mutant phenotype Ref.3. Source: MGI

regulation of respiratory gaseous exchange by neurological system process

Inferred from mutant phenotype Ref.5. Source: UniProtKB

sensory perception of touch

Inferred from mutant phenotype Ref.3. Source: MGI

smooth muscle tissue development

Inferred from mutant phenotype Ref.3. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ureter smooth muscle cell differentiation

Inferred from mutant phenotype Ref.3. Source: UniProtKB

ureteric bud development

Inferred from mutant phenotype Ref.3. Source: MGI

ureteric peristalsis

Inferred from mutant phenotype Ref.3. Source: UniProtKB

   Cellular_componentgrowth cone

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 18614015. Source: MGI

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionchromatin binding

Inferred from sequence or structural similarity. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10811081Teashirt homolog 3
PRO_0000047067

Regions

Zinc finger214 – 23825C2H2-type 1
Zinc finger275 – 29925C2H2-type 2
Zinc finger387 – 41024C2H2-type 3; atypical
DNA binding891 – 96171Homeobox; atypical
Zinc finger976 – 99823C2H2-type 4
Zinc finger1041 – 106424C2H2-type 5
Coiled coil606 – 63025 Potential
Compositional bias142 – 16423Ser-rich
Compositional bias493 – 4964Poly-Glu

Amino acid modifications

Modified residue6821Phosphoserine By similarity

Experimental info

Sequence conflict1101T → I in AAL38978. Ref.1
Sequence conflict6631S → G in BAD90255. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8CGV9 [UniParc].

Last modified January 10, 2006. Version 2.
Checksum: C69AD8A9A2816669

FASTA1,081118,626
        10         20         30         40         50         60 
MPRRKQQAPR RAAAYVSDEL KAAALVEDDV EPEEQAADGE PSAKYMCPEK ELSKACPSYQ 

        70         80         90        100        110        120 
NSPAAEFSSH EMDSESHISE TSDRMADFES SSIKNEEETK EVQVPLEDTT VSDSLEQMKA 

       130        140        150        160        170        180 
VYNNFLSNSY WSNLNLNLHQ PSSENNGGSS SSSSSSSSSC GSGSFDWHQS AMAKTLQQVS 

       190        200        210        220        230        240 
QNRMLPEPSL FSTVQLYRQS SKLYGSIFTG ASKFRCKDCS AAYDTLVELT VHMNETGHYR 

       250        260        270        280        290        300 
DDNHETDNNN PKRWSKPRKR SLLEMEGKED AQKVLKCMYC GHSFESLQDL SVHMIKTKHY 

       310        320        330        340        350        360 
QKVPLKEPVT PVAAKIIPAA RKKPSLELEL PSSPDSTGGT PKATLSDASD ALQKNSNPYI 

       370        380        390        400        410        420 
TPNNRYGHQN GASYAWHFEA RKSQILKCME CGSSHDTLQE LTAHMMVTGH FIKVTNSAMK 

       430        440        450        460        470        480 
KGKPIMETPV TPTITTLLDE KVQSVPLAAT TFTSPSNTPA SVSPKLAVEI KKEVDKEKAV 

       490        500        510        520        530        540 
PDEKPKEREK PSEEEEKYDI SSKYHYLTEN DLEESPKGGL DILKSLENTV TSAINKAQNG 

       550        560        570        580        590        600 
TPSWGGYPSI HAAYQLPNMM KLSLGSSGKS TPLKPMFGNS EIVSPTKTQT LVSPPSSQTS 

       610        620        630        640        650        660 
PMPKTNFHAM EELVKKVTEK VAKVEEKMKE PEGKLSPPKR ATPSPCSSEQ SEPIKMEASS 

       670        680        690        700        710        720 
DGSFKSQENS PSPPRDACKE ASPSAEPVEN GKELVKPLSG GLSGSTAIIT DHPPEQPFVN 

       730        740        750        760        770        780 
PLSALQSVMN IHLGKAAKPS LPALDPMSML FKMSNSLAEK AAVATPPPLQ AKKAEHLDRY 

       790        800        810        820        830        840 
FYHVNNDQPI DLTKGKSDKG CSLGSGLLSP TSTSPATSSS TVTTAKTSAV VSFMSNSPLR 

       850        860        870        880        890        900 
ENALSDISDM LKNLTESHTS KSSTPSSISE KSDIDGATLE EAEESTPAQK RKGRQSNWNP 

       910        920        930        940        950        960 
QHLLILQAQF AASLRQTSEG KYIMSDLSPQ ERMHISRFTG LSMTTISHWL ANVKYQLRRT 

       970        980        990       1000       1010       1020 
GGTKFLKNLD TGHPVFFCND CASQIRTPST YISHLESHLG FRLRDLSKLS TEQINNQIAQ 

      1030       1040       1050       1060       1070       1080 
TKSPSEKLVT SSPEEDLGTT YQCKLCNRTF ASKHAVKLHL SKTHGKSPED HLLFVSELEK 


Q 

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References

« Hide 'large scale' references
[1]"Three putative murine Teashirt orthologues specify trunk structures in Drosophila in the same way as the Drosophila teashirt gene."
Manfroid I., Caubit X., Kerridge S., Fasano L.
Development 131:1065-1073(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Fetal brain.
[3]"Teashirt 3 is necessary for ureteral smooth muscle differentiation downstream of SHH and BMP4."
Caubit X., Lye C.M., Martin E., Core N., Long D.A., Vola C., Jenkins D., Garratt A.N., Skaer H., Woolf A.S., Fasano L.
Development 135:3301-3310(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[4]"FE65 binds Teashirt, inhibiting expression of the primate-specific caspase-4."
Kajiwara Y., Akram A., Katsel P., Haroutunian V., Schmeidler J., Beecham G., Haines J.L., Pericak-Vance M.A., Buxbaum J.D.
PLoS ONE 4:E5071-E5071(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH APBB1.
[5]"Teashirt 3 regulates development of neurons involved in both respiratory rhythm and airflow control."
Caubit X., Thoby-Brisson M., Voituron N., Filippi P., Bevengut M., Faralli H., Zanella S., Fortin G., Hilaire G., Fasano L.
J. Neurosci. 30:9465-9476(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[6]"Analysis of TSHZ2 and TSHZ3 genes in congenital pelvi-ureteric junction obstruction."
Jenkins D., Caubit X., Dimovski A., Matevska N., Lye C.M., Cabuk F., Gucev Z., Tasic V., Fasano L., Woolf A.S.
Nephrol. Dial. Transplant. 25:54-60(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Life's first breath - Issue 122 of October 2010

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY063491 mRNA. Translation: AAL38978.1.
AK220396 mRNA. Translation: BAD90255.1. Frameshift.
RefSeqNP_758502.1. NM_172298.2.
UniGeneMm.44141.

3D structure databases

ProteinModelPortalQ8CGV9.
SMRQ8CGV9. Positions 200-303.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8CGV9.

Proteomic databases

PRIDEQ8CGV9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021641; ENSMUSP00000021641; ENSMUSG00000021217.
GeneID243931.
KEGGmmu:243931.
UCSCuc009gkj.1. mouse.

Organism-specific databases

CTD57616.
MGIMGI:2442819. Tshz3.
RougeSearch...

Phylogenomic databases

eggNOGNOG83709.
GeneTreeENSGT00390000014977.
HOGENOMHOG000231480.
HOVERGENHBG079626.
InParanoidQ8CGV9.
KOK09236.
OrthoDBEOG7NPFSF.
PhylomeDBQ8CGV9.
TreeFamTF328447.

Gene expression databases

ArrayExpressQ8CGV9.
BgeeQ8CGV9.
CleanExMM_TSHZ3.
GenevestigatorQ8CGV9.

Family and domain databases

InterProIPR001356. Homeobox_dom.
IPR027008. Teashirt_fam.
IPR026810. Tshz3.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERPTHR12487. PTHR12487. 1 hit.
PTHR12487:SF5. PTHR12487:SF5. 1 hit.
SMARTSM00389. HOX. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS50071. HOMEOBOX_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio386042.
PROQ8CGV9.
SOURCESearch...

Entry information

Entry nameTSH3_MOUSE
AccessionPrimary (citable) accession number: Q8CGV9
Secondary accession number(s): Q5DTX4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot