Q8CGV2 (TPH2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 5-hydroxylase 2
EC=1.14.16.4

Alternative name(s):
Neuronal tryptophan hydroxylase
Tryptophan 5-monooxygenase 2

Gene names

Name:	Tph2
Synonyms:	Ntph

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Tissue specificity	Brain specific.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	serotonin biosynthetic process from tryptophan Inferred by curator. Source: MGI
Cellular component	neuron projection Inferred from direct assay. Source: MGI
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro tryptophan 5-monooxygenase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Tryptophan 5-hydroxylase 2		PRO_0000205575
Regions
	Domain	62 – 134	73	ACT
Sites
	Metal binding	316	1	Iron By similarity
	Metal binding	321	1	Iron By similarity
	Metal binding	361	1	Iron By similarity
Amino acid modifications
	Modified residue	380	1	Phosphoserine By similarity
	Modified residue	390	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	447	1	P → R in AAM08923. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8CGV2 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 876B59C784E80E4A	FASTA	488	55,859
	10 20 30 40 50 60 MQPAMMMFSS KYWARRGLSL DSAVPEDHQL LGSLTQNKAI KSEDKKSGKE PGKGDTTESS 70 80 90 100 110 120 KTAVVFSLKN EVGGLVKALR LFQEKHVNML HIESRRSRRR SSEVEIFVDC ECGKTEFNEL 130 140 150 160 170 180 IQLLKFQTTI VTLNPPESIW TEEEDLEDVP WFPRKISELD RCSHRVLMYG TELDADHPGF 190 200 210 220 230 240 KDNVYRQRRK YFVDVAMGYK YGQPIPRVEY TEEETKTWGV VFRELSKLYP THACREYLKN 250 260 270 280 290 300 LPLLTKYCGY REDNVPQLED VSMFLKERSG FTVRPVAGYL SPRDFLAGLA YRVFHCTQYV 310 320 330 340 350 360 RHGSDPLYTP EPDTCHELLG HVPLLADPKF AQFSQEIGLA SLGASDEDVQ KLATCYFFTI 370 380 390 400 410 420 EFGLCKQEGQ LRAYGAGLLS SIGELKHALS DKACVKSFDP KTTCLQECLI TTFQDAYFVS 430 440 450 460 470 480 DSFEEAKEKM RDFAKSITRP FSVYFNPYTQ SIEILKDTRS IENVVQDLRS DLNTVCDALN KMNQYLGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Synthesis of serotonin by a second tryptophan hydroxylase isoform." Walther D.J., Peter J.-U., Bashammakh S., Hortnagl H., Voits M., Fink H., Bader M. Science 299:76-76(2003) [PubMed: 12511643] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY090565 mRNA. Translation: AAM08923.1. BC120514 mRNA. Translation: AAI20515.1. BC125430 mRNA. Translation: AAI25431.1.
IPI	IPI00229583.
RefSeq	NP_775567.2. NM_173391.3.
UniGene	Mm.31597.
3D structure databases
ProteinModelPortal	Q8CGV2.
SMR	Q8CGV2. Positions 53-482.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CGV2.
PTM databases
PhosphoSite	Q8CGV2.
Proteomic databases
PRIDE	Q8CGV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000006949; ENSMUSP00000006949; ENSMUSG00000006764.
GeneID	216343.
KEGG	mmu:216343.
Organism-specific databases
CTD	121278.
MGI	MGI:2651811. Tph2.
Phylogenomic databases
eggNOG	roNOG15466.
GeneTree	ENSGT00390000010268.
HOGENOM	HBG484724.
HOVERGEN	HBG006841.
InParanoid	Q8CGV2.
OrthoDB	EOG4S7JPZ.
Enzyme and pathway databases
BRENDA	1.14.16.4. 3474.
Gene expression databases
ArrayExpress	Q8CGV2.
Bgee	Q8CGV2.
CleanEx	MM_TPH2.
Genevestigator	Q8CGV2.
GermOnline	ENSMUSG00000006764. Mus musculus.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
KO	K00502.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
SUPFAM	SSF56534. Aaa_hydroxylase. 1 hit.
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BH4_AAA_HYDROXYL_1. 1 hit. PS51410. BH4_AAA_HYDROXYL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	375107.
SOURCE	Search...

Entry information

Entry name

TPH2_MOUSE

Accession

Primary (citable) accession number: Q8CGV2
Secondary accession number(s): Q0VBT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program