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Protein

Tryptophan 5-hydroxylase 2

Gene

Tph2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactori

Fe2+By similarity

Pathwayi: serotonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes serotonin from L-tryptophan.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Tryptophan 5-hydroxylase 1 (Tph1), Tryptophan 5-hydroxylase 2 (Tph2)
  2. no protein annotated in this organism
This subpathway is part of the pathway serotonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes serotonin from L-tryptophan, the pathway serotonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi316IronBy similarity1
Metal bindingi321IronBy similarity1
Metal bindingi361IronBy similarity1

GO - Molecular functioni

  • amino acid binding Source: InterPro
  • iron ion binding Source: InterPro
  • tryptophan 5-monooxygenase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Serotonin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.16.4. 3474.
ReactomeiR-MMU-209931. Serotonin and melatonin biosynthesis.
UniPathwayiUPA00846; UER00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 5-hydroxylase 2 (EC:1.14.16.4)
Alternative name(s):
Neuronal tryptophan hydroxylase
Tryptophan 5-monooxygenase 2
Gene namesi
Name:Tph2
Synonyms:Ntph
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2651811. Tph2.

Subcellular locationi

GO - Cellular componenti

  • neuron projection Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055751 – 488Tryptophan 5-hydroxylase 2Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CGV2.
PaxDbiQ8CGV2.
PRIDEiQ8CGV2.

PTM databases

iPTMnetiQ8CGV2.
PhosphoSitePlusiQ8CGV2.

Expressioni

Tissue specificityi

Brain specific.

Gene expression databases

BgeeiENSMUSG00000006764.
CleanExiMM_TPH2.
GenevisibleiQ8CGV2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006949.

Structurei

3D structure databases

ProteinModelPortaliQ8CGV2.
SMRiQ8CGV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 138ACTPROSITE-ProRule annotationAdd BLAST76

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3820. Eukaryota.
COG3186. LUCA.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiQ8CGV2.
KOiK00502.
OMAiKNDDKGN.
OrthoDBiEOG091G05MZ.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CGV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPAMMMFSS KYWARRGLSL DSAVPEDHQL LGSLTQNKAI KSEDKKSGKE
60 70 80 90 100
PGKGDTTESS KTAVVFSLKN EVGGLVKALR LFQEKHVNML HIESRRSRRR
110 120 130 140 150
SSEVEIFVDC ECGKTEFNEL IQLLKFQTTI VTLNPPESIW TEEEDLEDVP
160 170 180 190 200
WFPRKISELD RCSHRVLMYG TELDADHPGF KDNVYRQRRK YFVDVAMGYK
210 220 230 240 250
YGQPIPRVEY TEEETKTWGV VFRELSKLYP THACREYLKN LPLLTKYCGY
260 270 280 290 300
REDNVPQLED VSMFLKERSG FTVRPVAGYL SPRDFLAGLA YRVFHCTQYV
310 320 330 340 350
RHGSDPLYTP EPDTCHELLG HVPLLADPKF AQFSQEIGLA SLGASDEDVQ
360 370 380 390 400
KLATCYFFTI EFGLCKQEGQ LRAYGAGLLS SIGELKHALS DKACVKSFDP
410 420 430 440 450
KTTCLQECLI TTFQDAYFVS DSFEEAKEKM RDFAKSITRP FSVYFNPYTQ
460 470 480
SIEILKDTRS IENVVQDLRS DLNTVCDALN KMNQYLGI
Length:488
Mass (Da):55,859
Last modified:July 27, 2011 - v2
Checksum:i876B59C784E80E4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti447P → R in AAM08923 (PubMed:12511643).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090565 mRNA. Translation: AAM08923.1.
BC120514 mRNA. Translation: AAI20515.1.
BC125430 mRNA. Translation: AAI25431.1.
CCDSiCCDS36061.1.
RefSeqiNP_775567.2. NM_173391.3.
UniGeneiMm.31597.

Genome annotation databases

EnsembliENSMUST00000006949; ENSMUSP00000006949; ENSMUSG00000006764.
GeneIDi216343.
KEGGimmu:216343.
UCSCiuc007haw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY090565 mRNA. Translation: AAM08923.1.
BC120514 mRNA. Translation: AAI20515.1.
BC125430 mRNA. Translation: AAI25431.1.
CCDSiCCDS36061.1.
RefSeqiNP_775567.2. NM_173391.3.
UniGeneiMm.31597.

3D structure databases

ProteinModelPortaliQ8CGV2.
SMRiQ8CGV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006949.

PTM databases

iPTMnetiQ8CGV2.
PhosphoSitePlusiQ8CGV2.

Proteomic databases

MaxQBiQ8CGV2.
PaxDbiQ8CGV2.
PRIDEiQ8CGV2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006949; ENSMUSP00000006949; ENSMUSG00000006764.
GeneIDi216343.
KEGGimmu:216343.
UCSCiuc007haw.2. mouse.

Organism-specific databases

CTDi121278.
MGIiMGI:2651811. Tph2.

Phylogenomic databases

eggNOGiKOG3820. Eukaryota.
COG3186. LUCA.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiQ8CGV2.
KOiK00502.
OMAiKNDDKGN.
OrthoDBiEOG091G05MZ.
TreeFamiTF313327.

Enzyme and pathway databases

UniPathwayiUPA00846; UER00799.
BRENDAi1.14.16.4. 3474.
ReactomeiR-MMU-209931. Serotonin and melatonin biosynthesis.

Miscellaneous databases

PROiQ8CGV2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006764.
CleanExiMM_TPH2.
GenevisibleiQ8CGV2. MM.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPH2_MOUSE
AccessioniPrimary (citable) accession number: Q8CGV2
Secondary accession number(s): Q0VBT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.