Reviewed, UniProtKB/Swiss-Prot Q8CGV2 (TPH2_MOUSE)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase 2
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase 2
    Neuronal tryptophan hydroxylase

Gene names

Name:	Tph2
Synonyms:	Ntph

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Tissue specificity	Brain specific.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW serotonin biosynthetic process from tryptophan Inferred by curator. Source: MGI
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Tryptophan 5-hydroxylase 2		PRO_0000205575
Regions
	Domain	62 – 134	73	ACT
Sites
	Metal binding	316	1	Iron By similarity
	Metal binding	321	1	Iron By similarity
	Metal binding	361	1	Iron By similarity
Amino acid modifications
	Modified residue	380	1	Phosphoserine By similarity
	Modified residue	390	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CGV2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8769FB4DAEE80E4A	FASTA	488	55,918
	10 20 30 40 50 60 MQPAMMMFSS KYWARRGLSL DSAVPEDHQL LGSLTQNKAI KSEDKKSGKE PGKGDTTESS 70 80 90 100 110 120 KTAVVFSLKN EVGGLVKALR LFQEKHVNML HIESRRSRRR SSEVEIFVDC ECGKTEFNEL 130 140 150 160 170 180 IQLLKFQTTI VTLNPPESIW TEEEDLEDVP WFPRKISELD RCSHRVLMYG TELDADHPGF 190 200 210 220 230 240 KDNVYRQRRK YFVDVAMGYK YGQPIPRVEY TEEETKTWGV VFRELSKLYP THACREYLKN 250 260 270 280 290 300 LPLLTKYCGY REDNVPQLED VSMFLKERSG FTVRPVAGYL SPRDFLAGLA YRVFHCTQYV 310 320 330 340 350 360 RHGSDPLYTP EPDTCHELLG HVPLLADPKF AQFSQEIGLA SLGASDEDVQ KLATCYFFTI 370 380 390 400 410 420 EFGLCKQEGQ LRAYGAGLLS SIGELKHALS DKACVKSFDP KTTCLQECLI TTFQDAYFVS 430 440 450 460 470 480 DSFEEAKEKM RDFAKSITRP FSVYFNRYTQ SIEILKDTRS IENVVQDLRS DLNTVCDALN KMNQYLGI

References

[1]	"Synthesis of serotonin by a second tryptophan hydroxylase isoform." Walther D.J., Peter J.-U., Bashammakh S., Hortnagl H., Voits M., Fink H., Bader M. Science 299:76-76(2003) [PubMed: 12511643] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY090565 mRNA. Translation: AAM08923.1.
IPI	IPI00229583.
UniGene	Mm.31597
3D structure databases
SMR	Q8CGV2. Positions 57-127, 149-482.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8CGV2.
PTM databases
PhosphoSite	Q8CGV2.
Proteomic databases
PRIDE	Q8CGV2.
Genome annotation databases
Ensembl	ENSMUST00000006949; ENSMUSP00000006949; ENSMUSG00000006764; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:2651811. Tph2.
Phylogenomic databases
HOGENOM	HBG484724.
HOVERGEN	Q8CGV2.
InParanoid	Q8CGV2.
Enzyme and pathway databases
BRENDA	1.14.16.4. 244.
Gene expression databases
ArrayExpress	Q8CGV2.
Bgee	Q8CGV2.
CleanEx	MM_TPH2.
Genevestigator	Q8CGV2.
GermOnline	ENSMUSG00000006764. Mus musculus.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

TPH2_MOUSE

Accession

Primary (citable) accession number: Q8CGV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)