Reviewed, UniProtKB/Swiss-Prot Q8CGU9 (TPH2_RAT)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase 2
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase 2
    Neuronal tryptophan hydroxylase

Gene names

Name:	Tph2
Synonyms:	Ntph

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O(2) = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe(2+) ion By similarity.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW serotonin biosynthetic process from tryptophan Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro tryptophan 5-monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Tryptophan 5-hydroxylase 2		PRO_0000205576
Regions
	Domain	59 – 131	73	ACT
Sites
	Metal binding	313	1	Iron By similarity
	Metal binding	318	1	Iron By similarity
	Metal binding	358	1	Iron By similarity
Amino acid modifications
	Modified residue	377	1	Phosphoserine By similarity
	Modified residue	387	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CGU9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C859B87C04BE743D	FASTA	485	55,621
	10 20 30 40 50 60 MQPAMMMFSS KYWARRGLSL DSAVPEEHQI LGGLTQNKAT ASKSEDKRSG KDTSESSKTA 70 80 90 100 110 120 VVFSLKNEVG GLVRALRLFQ EKHVNMLHIE SRRSRRRSSE VEIFVDCECG KTEFNELIQL 130 140 150 160 170 180 LKFQTTIVTL NPPDNIWTEE EELEDVPWFP RKISELDRCS HRVLMYGTEL DADHPGFKDN 190 200 210 220 230 240 VYRQRRKYFV DVAMGYKYGQ PIPRVEYTEE ETKTWGVVFR ELSKLYPTHA CREYLKNFPL 250 260 270 280 290 300 LTKYCGYRED NVPQLEDVSM FLKERSGFTV RPVAGYLSPR DFLAGLAYRV FHCTQYVRHG 310 320 330 340 350 360 SDPLYTPEPD TCHELLGHVP LLADPKFAQF SQEIGLASLG ASDEDVQKLA TCYFFTIEFG 370 380 390 400 410 420 LCKQEGQLRA YGAGLLSSIG ELKHALSDKA CVKAFDPKTT CLQECLITTF QDAYFVSESF 430 440 450 460 470 480 EEAKEKMRDF AKSITRPFSV YFNPYTQSIE ILKDTRSIEN VVQDLRSDLN TVCDALNKMN QYLGI

References

[1]	"Synthesis of serotonin by a second tryptophan hydroxylase isoform." Walther D.J., Peter J.-U., Bashammakh S., Hortnagl H., Voits M., Fink H., Bader M. Science 299:76-76(2003) [PubMed: 12511643] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY098915 mRNA. Translation: AAM28947.1.
RefSeq	NP_776211.1.
UniGene	Rn.28510
3D structure databases
HSSP	HSSP built from PDB template 1TOH based on UniProtKB P04177.
SMR	Q8CGU9. Positions 146-434.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000003880. Rattus norvegicus. [Contig view]
GeneID	317675.
KEGG	rno:317675.
Organism-specific databases
RGD	631332. Tph2.
Phylogenomic databases
HOVERGEN	Q8CGU9.
Gene expression databases
GermOnline	ENSRNOG00000003880. Rattus norvegicus.
Family and domain databases
InterPro	IPR001273. Aaa_hydroxylase. IPR002912. ACT_bd. IPR005963. Tyr_5_mOase. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q8CGU9.
NextBio	672069.

Entry information

Entry name

TPH2_RAT

Accession

Primary (citable) accession number: Q8CGU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	March 1, 2003
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)