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Protein

Tryptophan 5-hydroxylase 2

Gene

Tph2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactori

Fe2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi313 – 3131IronBy similarity
Metal bindingi318 – 3181IronBy similarity
Metal bindingi358 – 3581IronBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. tryptophan 5-monooxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. aromatic amino acid family metabolic process Source: InterPro
  2. cellular response to lithium ion Source: RGD
  3. circadian rhythm Source: RGD
  4. response to activity Source: RGD
  5. response to calcium ion Source: RGD
  6. response to estrogen Source: RGD
  7. response to glucocorticoid Source: RGD
  8. response to nutrient levels Source: RGD
  9. serotonin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Serotonin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_259603. Serotonin and melatonin biosynthesis.
SABIO-RKQ8CGU9.
UniPathwayiUPA00846; UER00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 5-hydroxylase 2 (EC:1.14.16.4)
Alternative name(s):
Neuronal tryptophan hydroxylase
Tryptophan 5-monooxygenase 2
Gene namesi
Name:Tph2
Synonyms:Ntph
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi631332. Tph2.

Subcellular locationi

GO - Cellular componenti

  1. neuron projection Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Tryptophan 5-hydroxylase 2PRO_0000205576Add
BLAST

Proteomic databases

PRIDEiQ8CGU9.

Expressioni

Gene expression databases

GenevestigatoriQ8CGU9.

Interactioni

Protein-protein interaction databases

IntActiQ8CGU9. 1 interaction.
MINTiMINT-7997028.
STRINGi10116.ENSRNOP00000005157.

Structurei

3D structure databases

ProteinModelPortaliQ8CGU9.
SMRiQ8CGU9. Positions 146-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 13576ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiQ8CGU9.
KOiK00502.
OMAiHACKQFL.
OrthoDBiEOG7KM5T1.
PhylomeDBiQ8CGU9.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CGU9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQPAMMMFSS KYWARRGLSL DSAVPEEHQI LGGLTQNKAT ASKSEDKRSG
60 70 80 90 100
KDTSESSKTA VVFSLKNEVG GLVRALRLFQ EKHVNMLHIE SRRSRRRSSE
110 120 130 140 150
VEIFVDCECG KTEFNELIQL LKFQTTIVTL NPPDNIWTEE EELEDVPWFP
160 170 180 190 200
RKISELDRCS HRVLMYGTEL DADHPGFKDN VYRQRRKYFV DVAMGYKYGQ
210 220 230 240 250
PIPRVEYTEE ETKTWGVVFR ELSKLYPTHA CREYLKNFPL LTKYCGYRED
260 270 280 290 300
NVPQLEDVSM FLKERSGFTV RPVAGYLSPR DFLAGLAYRV FHCTQYVRHG
310 320 330 340 350
SDPLYTPEPD TCHELLGHVP LLADPKFAQF SQEIGLASLG ASDEDVQKLA
360 370 380 390 400
TCYFFTIEFG LCKQEGQLRA YGAGLLSSIG ELKHALSDKA CVKAFDPKTT
410 420 430 440 450
CLQECLITTF QDAYFVSESF EEAKEKMRDF AKSITRPFSV YFNPYTQSIE
460 470 480
ILKDTRSIEN VVQDLRSDLN TVCDALNKMN QYLGI
Length:485
Mass (Da):55,621
Last modified:March 1, 2003 - v1
Checksum:iC859B87C04BE743D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY098915 mRNA. Translation: AAM28947.1.
RefSeqiNP_776211.1. NM_173839.2.
UniGeneiRn.28510.

Genome annotation databases

EnsembliENSRNOT00000005157; ENSRNOP00000005157; ENSRNOG00000003880.
GeneIDi317675.
KEGGirno:317675.
UCSCiRGD:631332. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY098915 mRNA. Translation: AAM28947.1.
RefSeqiNP_776211.1. NM_173839.2.
UniGeneiRn.28510.

3D structure databases

ProteinModelPortaliQ8CGU9.
SMRiQ8CGU9. Positions 146-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CGU9. 1 interaction.
MINTiMINT-7997028.
STRINGi10116.ENSRNOP00000005157.

Proteomic databases

PRIDEiQ8CGU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005157; ENSRNOP00000005157; ENSRNOG00000003880.
GeneIDi317675.
KEGGirno:317675.
UCSCiRGD:631332. rat.

Organism-specific databases

CTDi121278.
RGDi631332. Tph2.

Phylogenomic databases

eggNOGiCOG3186.
GeneTreeiENSGT00390000010268.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiQ8CGU9.
KOiK00502.
OMAiHACKQFL.
OrthoDBiEOG7KM5T1.
PhylomeDBiQ8CGU9.
TreeFamiTF313327.

Enzyme and pathway databases

UniPathwayiUPA00846; UER00799.
ReactomeiREACT_259603. Serotonin and melatonin biosynthesis.
SABIO-RKQ8CGU9.

Miscellaneous databases

NextBioi672069.

Gene expression databases

GenevestigatoriQ8CGU9.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Synthesis of serotonin by a second tryptophan hydroxylase isoform."
    Walther D.J., Peter J.-U., Bashammakh S., Hortnagl H., Voits M., Fink H., Bader M.
    Science 299:76-76(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTPH2_RAT
AccessioniPrimary (citable) accession number: Q8CGU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.