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Protein

Charged multivesicular body protein 3

Gene

Chmp3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei48 – 481Important for autoinhibitory functionBy similarity
Binding sitei217 – 2171STAMBPBy similarity

GO - Molecular functioni

  • phosphatidylinositol-4,5-bisphosphate binding Source: RGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • endosome to lysosome transport Source: RGD
  • positive regulation of cytokinesis Source: RGD
  • protein transport Source: UniProtKB-KW
  • regulation of endosome size Source: RGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-1632852. Macroautophagy.
R-RNO-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 3
Alternative name(s):
Chromatin-modifying protein 3
Vacuolar protein sorting-associated protein 24
Short name:
rVps24p
Gene namesi
Name:Chmp3
Synonyms:Vps24
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi708556. Chmp3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • early endosome Source: RGD
  • ESCRT III complex Source: RGD
  • late endosome Source: RGD
  • late endosome membrane Source: UniProtKB-SubCell
  • midbody Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491K → D: Strongly reduces binding to PtdIns(3,5)P2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 223222Charged multivesicular body protein 3PRO_0000211483Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Cross-linki179 – 179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei200 – 2001PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8CGS4.
PRIDEiQ8CGS4.

PTM databases

iPTMnetiQ8CGS4.
PhosphoSiteiQ8CGS4.

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Forms a metastable monomer in solution; its core structure (without part of the putative autoinhibitory C-terminal acidic region) oligomerizes into a flat lattice via two different dimerization interfaces. In vitro, heteromerizes with CHMP2A (but not CHMP4) to form helical tubular structures that expose membrane-interacting sites on the outside whereas VPS4B can associate on the inside of the tubule. May interact with IGFBP7; the relevance of such interaction however remains unclear. Interacts with CHMP2A. Interacts with CHMP4A; the interaction requires the release of CHMP4A autoinhibition. Interacts with VPS4A. Interacts with STAMBP; the interaction appears to relieve the autoinhibition of CHMP3 (By similarity). Interacts with VTA1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010253.

Structurei

3D structure databases

ProteinModelPortaliQ8CGS4.
SMRiQ8CGS4. Positions 5-180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 113112Intramolecular interaction with C-terminusBy similarityAdd
BLAST
Regioni59 – 646Important for autoinhibitory functionBy similarity
Regioni151 – 22373Interaction with VPS4ABy similarityAdd
BLAST
Regioni151 – 22171Intramolecular interaction with N-terminusBy similarityAdd
BLAST
Regioni168 – 1692Important for autoinhibitory functionBy similarity
Regioni196 – 22328Interaction with STAMBPBy similarityAdd
BLAST
Regioni204 – 2085Interaction with STAMBP
Regioni222 – 2232Interaction with STAMBP

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili22 – 5433Sequence analysisAdd
BLAST
Coiled coili149 – 22375Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 21212MIT-interacting motifBy similarityAdd
BLAST

Domaini

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3229. Eukaryota.
COG5491. LUCA.
GeneTreeiENSGT00550000074896.
HOGENOMiHOG000177219.
HOVERGENiHBG107031.
InParanoidiQ8CGS4.
KOiK12193.
OMAiQKDVCVI.
OrthoDBiEOG7D59Q2.
PhylomeDBiQ8CGS4.
TreeFamiTF105848.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CGS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD
60 70 80 90 100
AAKKGQKEVC VVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV
110 120 130 140 150
AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES
160 170 180 190 200
MDDQEEMEEA AEMEIDRILF EITAGALGKA PSKVTDALPE PEPAGAMAAS
210 220
EGDEEDDEED LEAMQSRLAT LRS
Length:223
Mass (Da):25,062
Last modified:January 23, 2007 - v3
Checksum:i203CCA75C78E4586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY150169 mRNA. Translation: AAN74982.1.
RefSeqiNP_758834.1. NM_172331.1.
UniGeneiRn.162751.

Genome annotation databases

EnsembliENSRNOT00000010253; ENSRNOP00000010253; ENSRNOG00000007356.
GeneIDi282834.
KEGGirno:282834.
UCSCiRGD:708556. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY150169 mRNA. Translation: AAN74982.1.
RefSeqiNP_758834.1. NM_172331.1.
UniGeneiRn.162751.

3D structure databases

ProteinModelPortaliQ8CGS4.
SMRiQ8CGS4. Positions 5-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010253.

PTM databases

iPTMnetiQ8CGS4.
PhosphoSiteiQ8CGS4.

Proteomic databases

PaxDbiQ8CGS4.
PRIDEiQ8CGS4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010253; ENSRNOP00000010253; ENSRNOG00000007356.
GeneIDi282834.
KEGGirno:282834.
UCSCiRGD:708556. rat.

Organism-specific databases

CTDi51652.
RGDi708556. Chmp3.

Phylogenomic databases

eggNOGiKOG3229. Eukaryota.
COG5491. LUCA.
GeneTreeiENSGT00550000074896.
HOGENOMiHOG000177219.
HOVERGENiHBG107031.
InParanoidiQ8CGS4.
KOiK12193.
OMAiQKDVCVI.
OrthoDBiEOG7D59Q2.
PhylomeDBiQ8CGS4.
TreeFamiTF105848.

Enzyme and pathway databases

ReactomeiR-RNO-1632852. Macroautophagy.
R-RNO-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

NextBioi624897.
PROiQ8CGS4.

Family and domain databases

InterProiIPR005024. Snf7_fam.
[Graphical view]
PfamiPF03357. Snf7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of mammalian Vps24p as an effector of phosphatidylinositol 3,5-bisphosphate-dependent endosome compartmentalization."
    Whitley P., Reaves B.J., Hashimoto M., Riley A.M., Potter B.V.L., Holman G.D.
    J. Biol. Chem. 278:38786-38795(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-49.
    Strain: Wistar.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-200, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCHMP3_RAT
AccessioniPrimary (citable) accession number: Q8CGS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.