Q8CGP2 (H2B1P_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone H2B type 1-P

Gene names

Name:

Hist1h2bp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subunit structure	The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Subcellular location	Nucleus. Chromosome.
Post-translational modification	Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II By similarity. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Ref.5 Ref.6 Ref.7
Miscellaneous	The human orthologous protein seems not to exist.
Sequence similarities	Belongs to the histone H2B family.

Ontologies

Keywords
Cellular component	Chromosome Nucleosome core Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Acetylation Isopeptide bond Methylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleosome assembly Inferred from electronic annotation. Source: InterPro
Cellular component	nucleosome Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CGP2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CGP2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     126-126: K → KILWNKFYYLPSF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 126	125	Histone H2B type 1-P		PRO_0000244834
Amino acid modifications
	Modified residue	6	1	N6-acetyllysine By similarity
	Modified residue	12	1	N6-acetyllysine By similarity
	Modified residue	13	1	N6-acetyllysine By similarity
	Modified residue	15	1	Phosphoserine; by STK4/MST1 Ref.5 Ref.6
	Modified residue	16	1	N6-acetyllysine By similarity
	Modified residue	17	1	N6-acetyllysine By similarity
	Modified residue	21	1	N6-acetyllysine By similarity
	Modified residue	24	1	N6-acetyllysine By similarity
	Modified residue	37	1	Phosphoserine; by AMPK Ref.7
	Modified residue	47	1	N6-methylated lysine By similarity
	Modified residue	58	1	N6-methylated lysine By similarity
	Modified residue	109	1	N6-acetyllysine; alternate By similarity
	Modified residue	109	1	N6-methylated lysine; alternate By similarity
	Cross-link	121		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Natural variations
	Alternative sequence	126	1	K → KILWNKFYYLPSF in isoform 2.		VSP_019618
Experimental info
	Sequence conflict	6	1	K → R in AAA50377. Ref.3
	Sequence conflict	76	1	S → G in AAA50377. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 2DE94CFE88C8BE0E	FASTA	126	13,992
	10 20 30 40 50 60 MPEPVKSVPA PKKGSKKAVT KAQKKDGKKR KRSRKESYSV YVYKVLKQVH PDTGISSKAM 70 80 90 100 110 120 GIMNSFVNDI FERIASEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSSK
	Isoform 2 [UniParc]. Checksum: DC31498A9C350E12	FASTA	138	15,565
	10 20 30 40 50 60 MPEPVKSVPA PKKGSKKAVT KAQKKDGKKR KRSRKESYSV YVYKVLKQVH PDTGISSKAM 70 80 90 100 110 120 GIMNSFVNDI FERIASEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT 130 KYTSSKILWN KFYYLPSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human and mouse replication-dependent histone genes." Marzluff W.F., Gongidi P., Woods K.R., Jin J., Maltais L.J. Genomics 80:487-498(2002) [PubMed: 12408966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 1).
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[4]	"Expression of a novel histone 2B during mouse spermiogenesis." Moss S.B., Challoner P.B., Groudine M. Dev. Biol. 133:83-92(1989) [PubMed: 2707490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-126 (ISOFORM 2). Strain: Swiss Webster. Tissue: Testis.
[5]	"Phosphorylation of histone H2B at DNA double-strand breaks." Fernandez-Capetillo O., Allis C.D., Nussenzweig A. J. Exp. Med. 199:1671-1677(2004) [PubMed: 15197225] [Abstract] Cited for: PHOSPHORYLATION AT SER-15.
[6]	"Histone modifications associated with somatic hypermutation." Odegard V.H., Kim S.T., Anderson S.M., Shlomchik M.J., Schatz D.G. Immunity 23:101-110(2005) [PubMed: 16039583] [Abstract] Cited for: PHOSPHORYLATION AT SER-15.
[7]	"Signaling kinase AMPK activates stress-promoted transcription via histone H2B phosphorylation." Bungard D., Fuerth B.J., Zeng P.Y., Faubert B., Maas N.L., Viollet B., Carling D., Thompson C.B., Jones R.G., Berger S.L. Science 329:1201-1205(2010) [PubMed: 20647423] [Abstract] Cited for: PHOSPHORYLATION AT SER-37.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY158930 Genomic DNA. Translation: AAO06240.1. AL589651 Genomic DNA. Translation: CAI24115.1. AL589651 Genomic DNA. Translation: CAI24116.1. BC061044 mRNA. Translation: AAH61044.1. M25487 mRNA. Translation: AAA50377.1.
IPI	IPI00648991. IPI00761713.
PIR	A37363.
RefSeq	NP_835509.2. NM_178202.2.
UniGene	Mm.264645.
3D structure databases
ProteinModelPortal	Q8CGP2.
SMR	Q8CGP2. Positions 6-126.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8CGP2. 1 interaction.
Proteomic databases
PRIDE	Q8CGP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102982; ENSMUSP00000100047; ENSMUSG00000069308. ENSMUST00000110473; ENSMUSP00000106099; ENSMUSG00000069308.
GeneID	319188.
KEGG	mmu:319188.
UCSC	uc007jcr.1. mouse.
Organism-specific databases
CTD	319188.
MGI	MGI:2448409. Hist1h2bp.
Phylogenomic databases
eggNOG	roNOG16405.
GeneTree	ENSGT00600000084028.
HOVERGEN	HBG007774.
OrthoDB	EOG4FN4K9.
PhylomeDB	Q8CGP2.
Enzyme and pathway databases
Reactome	REACT_27235. Meiotic Recombination (mouse). REACT_75800. Meiotic Synapsis (mouse).
Gene expression databases
ArrayExpress	Q8CGP2.
Bgee	Q8CGP2.
CleanEx	MM_HIST1H2BP.
Genevestigator	Q8CGP2.
GermOnline	ENSMUSG00000069268. Mus musculus. ENSMUSG00000069271. Mus musculus. ENSMUSG00000069300. Mus musculus. ENSMUSG00000069314. Mus musculus.
Family and domain databases
InterPro	IPR009072. Histone-fold. IPR007125. Histone_core_D. IPR000558. Histone_H2B. [Graphical view]
Gene3D	G3DSA:1.10.20.10. Histone-fold. 1 hit.
KO	K11252.
PANTHER	PTHR23428. Histone_H2B. 1 hit.
Pfam	PF00125. Histone. 1 hit. [Graphical view]
PRINTS	PR00621. HISTONEH2B.
SMART	SM00427. H2B. 1 hit. [Graphical view]
SUPFAM	SSF47113. Histone-fold. 1 hit.
PROSITE	PS00357. HISTONE_H2B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	394334.
SOURCE	Search...

Entry information

Entry name

H2B1P_MOUSE

Accession

Primary (citable) accession number: Q8CGP2
Secondary accession number(s): Q64477, Q6P8V8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program