Q8CGN5 (PLIN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Perilipin-1

Alternative name(s):
Lipid droplet-associated protein
Perilipin A

Gene names

Name:	Plin1
Synonyms:	Peri, Plin

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness By similarity.
Subunit structure	Interacts with ABHD5. Ref.6
Subcellular location	Lipid droplet By similarity. Note: Lipid droplet surface-associated By similarity.
Post-translational modification	Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet By similarity. Ref.7
Sequence similarities	Belongs to the perilipin family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Lipid droplet
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from mutant phenotype PubMed 11371650. Source: MGI
Cellular_component	lipid particle Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8CGN5-1)

Also known as: Peri A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8CGN5-2)

Also known as: Peri B;

The sequence of this isoform differs from the canonical sequence as follows:
     406-421: LPRLSLMEPESEFRDI → VSPAPGAPSDSQGFRD
     422-517: Missing.

Isoform 3 (identifier: Q8CGN5-3)

Also known as: Peri C;

The sequence of this isoform differs from the canonical sequence as follows:
     326-347: ALPNPRGLLGGVVHTVQNTLRN → KTESPRPHSPRLGGEKEGNGIE
     348-517: Missing.

Isoform 4 (identifier: Q8CGN5-4)

Also known as: Peri D;

The sequence of this isoform differs from the canonical sequence as follows:
     199-244: APSSGRQRTQ...TTAWALKQGH → GICHPDWLAI...GYPSASSGNS
     245-517: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Perilipin-1		PRO_0000099885
Regions
	Compositional bias	308 – 319	12	Poly-Glu
Amino acid modifications
	Modified residue	410	1	Phosphoserine Ref.7
	Modified residue	460	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	199 – 244	46	APSSG…LKQGH → GICHPDWLAILGARWPLSTR GSVGGIQVLLALLWDRGYPS ASSGNS in isoform 4.		VSP_038204
	Alternative sequence	245 – 517	273	Missing in isoform 4.		VSP_038205
	Alternative sequence	326 – 347	22	ALPNP…NTLRN → KTESPRPHSPRLGGEKEGNG IE in isoform 3.		VSP_038206
	Alternative sequence	348 – 517	170	Missing in isoform 3.		VSP_038207
	Alternative sequence	406 – 421	16	LPRLS…EFRDI → VSPAPGAPSDSQGFRD in isoform 2.		VSP_038208
	Alternative sequence	422 – 517	96	Missing in isoform 2.		VSP_038209
Experimental info
	Sequence conflict	3	1	M → I in AAN77870. Ref.2
	Sequence conflict	317	1	E → D in BAC27409. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Peri A) [UniParc]. Last modified October 13, 2009. Version 2. Checksum: 906AE1CC5AFD65AE	FASTA	517	55,596
	10 20 30 40 50 60 MSMNKGPTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YNSTKEAHPL VASVCNAYEK 70 80 90 100 110 120 GVQGASNLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE KIPALQYPPE KIASELKGTI 130 140 150 160 170 180 STRLRSARNS ISVPIASTSD KVLGATLAGC ELALGMAKET AEYAANTRVG RLASGGADLA 190 200 210 220 230 240 LGSIEKVVEF LLPPDKESAP SSGRQRTQKA PKAKPSLVRR VSTLANTLSR HTMQTTAWAL 250 260 270 280 290 300 KQGHSLAMWI PGVAPLSSLA QWGASAAMQV VSRRQSEVRV PWLHNLAASQ DESHDDQTDT 310 320 330 340 350 360 EGEETDDEEE EEESEAEENV LREVTALPNP RGLLGGVVHT VQNTLRNTIS AVTWAPAAVL 370 380 390 400 410 420 GTVGRILHLT PAQAVSSTKG RAMSLSDALK GVTDNVVDTV VHYVPLPRLS LMEPESEFRD 430 440 450 460 470 480 IDNPSAEAER KGSGARPASP ESTPRPGQPR GSLRSVRGLS APSCPGLDDK TEASARPGFL 490 500 510 AMPREKPARR VSDSFFRPSV MEPILGRAQY SQLRKKS
	Isoform 2 (Peri B) [UniParc]. Checksum: D095B9EBBA5F2A33	FASTA	421	44,936
	10 20 30 40 50 60 MSMNKGPTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YNSTKEAHPL VASVCNAYEK 70 80 90 100 110 120 GVQGASNLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE KIPALQYPPE KIASELKGTI 130 140 150 160 170 180 STRLRSARNS ISVPIASTSD KVLGATLAGC ELALGMAKET AEYAANTRVG RLASGGADLA 190 200 210 220 230 240 LGSIEKVVEF LLPPDKESAP SSGRQRTQKA PKAKPSLVRR VSTLANTLSR HTMQTTAWAL 250 260 270 280 290 300 KQGHSLAMWI PGVAPLSSLA QWGASAAMQV VSRRQSEVRV PWLHNLAASQ DESHDDQTDT 310 320 330 340 350 360 EGEETDDEEE EEESEAEENV LREVTALPNP RGLLGGVVHT VQNTLRNTIS AVTWAPAAVL 370 380 390 400 410 420 GTVGRILHLT PAQAVSSTKG RAMSLSDALK GVTDNVVDTV VHYVPVSPAP GAPSDSQGFR D
	Isoform 3 (Peri C) [UniParc]. Checksum: 1F196F6ADD7BC77F	FASTA	347	37,489
	10 20 30 40 50 60 MSMNKGPTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YNSTKEAHPL VASVCNAYEK 70 80 90 100 110 120 GVQGASNLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE KIPALQYPPE KIASELKGTI 130 140 150 160 170 180 STRLRSARNS ISVPIASTSD KVLGATLAGC ELALGMAKET AEYAANTRVG RLASGGADLA 190 200 210 220 230 240 LGSIEKVVEF LLPPDKESAP SSGRQRTQKA PKAKPSLVRR VSTLANTLSR HTMQTTAWAL 250 260 270 280 290 300 KQGHSLAMWI PGVAPLSSLA QWGASAAMQV VSRRQSEVRV PWLHNLAASQ DESHDDQTDT 310 320 330 340 EGEETDDEEE EEESEAEENV LREVTKTESP RPHSPRLGGE KEGNGIE
	Isoform 4 (Peri D) [UniParc]. Checksum: 3F65AEC4A4C9D796	FASTA	244	25,934
	10 20 30 40 50 60 MSMNKGPTLL DGDLPEQENV LQRVLQLPVV SGTCECFQKT YNSTKEAHPL VASVCNAYEK 70 80 90 100 110 120 GVQGASNLAA WSMEPVVRRL STQFTAANEL ACRGLDHLEE KIPALQYPPE KIASELKGTI 130 140 150 160 170 180 STRLRSARNS ISVPIASTSD KVLGATLAGC ELALGMAKET AEYAANTRVG RLASGGADLA 190 200 210 220 230 240 LGSIEKVVEF LLPPDKESGI CHPDWLAILG ARWPLSTRGS VGGIQVLLAL LWDRGYPSAS SGNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine perilipin gene: the lipid droplet-associated perilipins derive from tissue-specific, mRNA splice variants and define a gene family of ancient origin." Lu X., Gruia-Gray J., Copeland N.G., Gilbert D.J., Jenkins N.A., Londos C., Kimmel A.R. Mamm. Genome 12:741-749(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
[2]	"Mouse perilipin." Tansey J.T., Lu X., Londos C., Kimmel A.R. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Mammary gland.
[6]	"Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytes." Subramanian V., Rothenberg A., Gomez C., Cohen A.W., Garcia A., Bhattacharyya S., Shapiro L., Dolios G., Wang R., Lisanti M.P., Brasaemle D.L. J. Biol. Chem. 279:42062-42071(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ABHD5.
[7]	"The stoichiometry of protein phosphorylation in adipocyte lipid droplets: analysis by N-terminal isotope tagging and enzymatic dephosphorylation." Kanshin E., Wang S., Ashmarina L., Fedjaev M., Nifant'ev I., Mitchell G.A., Pshezhetsky A.V. Proteomics 9:5067-5077(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-410 AND SER-460.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Fat, wonderful fat - Issue 10 of May 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY161165 mRNA. Translation: AAN77870.1. AK031445 mRNA. Translation: BAC27409.1. CH466543 Genomic DNA. Translation: EDL07055.1. BC096685 mRNA. Translation: AAH96685.1.
IPI	IPI00223783. IPI00947590. IPI00947605. IPI00947617.
RefSeq	NP_001106942.1. NM_001113471.1. NP_783571.2. NM_175640.2.
UniGene	Mm.254917.
3D structure databases
ProteinModelPortal	Q8CGN5.
ModBase	Search...
PTM databases
PhosphoSite	Q8CGN5.
Proteomic databases
PaxDb	Q8CGN5.
PRIDE	Q8CGN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032762; ENSMUSP00000032762; ENSMUSG00000030546. ENSMUST00000178257; ENSMUSP00000136996; ENSMUSG00000030546.
GeneID	103968.
KEGG	mmu:103968.
UCSC	uc009hyu.2. mouse. uc009hyw.1. mouse.
Organism-specific databases
CTD	5346.
MGI	MGI:1890505. Plin1.
Phylogenomic databases
eggNOG	NOG75006.
GeneTree	ENSGT00500000044795.
HOGENOM	HOG000261608.
InParanoid	Q4V9U2.
KO	K08768.
OMA	DSFFRPS.
OrthoDB	EOG483D4G.
Enzyme and pathway databases
Reactome	REACT_112621. Metabolism.
Gene expression databases
Bgee	Q8CGN5.
CleanEx	MM_PLIN.
Genevestigator	Q8CGN5.
GermOnline	ENSMUSG00000030546. Mus musculus.
Family and domain databases
InterPro	IPR004279. Perilipin. [Graphical view]
PANTHER	PTHR14024. PTHR14024. 1 hit.
Pfam	PF03036. Perilipin. 1 hit. [Graphical view]
PIRSF	PIRSF036881. PAT. 1 hit.
ProtoNet	Search...
Other
NextBio	356265.
SOURCE	Search...

Entry information

Entry name

PLIN1_MOUSE

Accession

Primary (citable) accession number: Q8CGN5
Secondary accession number(s): Q4V9U2, Q8C0F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	October 13, 2009
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program