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Protein

Adhesion G protein-coupled receptor B2

Gene

Adgrb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Orphan receptor involved in cell adhesion and probably in cell-cell interactions. Might be involved in angiogenesis inhibition (PubMed:12218411) Activates NFAT signaling pathway, a transcription factor, probably via a G-protein dependent pathway (By similarity).By similarity1 Publication

Enzyme regulationi

Displays increased activity following removal of N-terminal regions (mimicking the situation after proteolytic cleavage in the GPS domain and N-terminal fragment release).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Protein family/group databases

MEROPSiP02.029.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor B2
Alternative name(s):
Brain-specific angiogenesis inhibitor 2
Gene namesi
Name:Adgrb2
Synonyms:Bai2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2451244. Adgrb2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 930ExtracellularCuratedAdd BLAST910
Transmembranei931 – 951Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini952 – 959CytoplasmicCurated8
Transmembranei960 – 980Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini981 – 988ExtracellularCurated8
Transmembranei989 – 1009Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini1010 – 1030CytoplasmicCuratedAdd BLAST21
Transmembranei1031 – 1051Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini1052 – 1072ExtracellularCuratedAdd BLAST21
Transmembranei1073 – 1093Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini1094 – 1115CytoplasmicCuratedAdd BLAST22
Transmembranei1116 – 1136Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1137 – 1147ExtracellularCuratedAdd BLAST11
Transmembranei1148 – 1168Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1169 – 1561CytoplasmicCuratedAdd BLAST393

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice show significant resistance to depression after repeated stress in the social defeat test. Additionally, hippocampal cell proliferation in deficient mice is increased.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000024561321 – 1561Adhesion G protein-coupled receptor B2Add BLAST1541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...)Sequence analysis1
Glycosylationi182N-linked (GlcNAc...)Sequence analysis1
Glycosylationi183N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi312 ↔ 346PROSITE-ProRule annotation
Disulfide bondi316 ↔ 352PROSITE-ProRule annotation
Disulfide bondi327 ↔ 336PROSITE-ProRule annotation
Glycosylationi347N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi367 ↔ 402PROSITE-ProRule annotation
Disulfide bondi371 ↔ 407PROSITE-ProRule annotation
Disulfide bondi382 ↔ 392PROSITE-ProRule annotation
Disulfide bondi422 ↔ 457PROSITE-ProRule annotation
Disulfide bondi426 ↔ 462PROSITE-ProRule annotation
Glycosylationi428N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi437 ↔ 447PROSITE-ProRule annotation
Disulfide bondi478 ↔ 513PROSITE-ProRule annotation
Disulfide bondi482 ↔ 518PROSITE-ProRule annotation
Disulfide bondi493 ↔ 503PROSITE-ProRule annotation
Disulfide bondi525 ↔ 560PROSITE-ProRule annotation
Disulfide bondi548 ↔ 578PROSITE-ProRule annotation
Glycosylationi551N-linked (GlcNAc...)Sequence analysis1
Glycosylationi636N-linked (GlcNAc...)Sequence analysis1
Glycosylationi861N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi868 ↔ 900PROSITE-ProRule annotation
Disulfide bondi888 ↔ 902PROSITE-ProRule annotation
Modified residuei1345PhosphotyrosineCombined sources1

Post-translational modificationi

Proteolytically cleaved into 2 conserved sites: one in the GPS domain (S1 site) and the other in the middle of the extracellular domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. Furin is involved in the cleavage of the S2 site generating a soluble fragment.By similarity
Glycosylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei287 – 288Cleavage; by furinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8CGM1.
PRIDEiQ8CGM1.

PTM databases

iPTMnetiQ8CGM1.
PhosphoSitePlusiQ8CGM1.

Expressioni

Tissue specificityi

Specifically expressed in the brain. The peak level in the brain is observed 10 days after birth.1 Publication

Developmental stagei

Ubiquitous in embryonic tissues, but expression is acutely down-regulated after birth, except in the brain, to a level that is maintained throughout adulthood.1 Publication

Inductioni

Down-regulated after hypoxia.

Gene expression databases

BgeeiENSMUSG00000028782.
CleanExiMM_BAI2.
ExpressionAtlasiQ8CGM1. baseline and differential.
GenevisibleiQ8CGM1. MM.

Interactioni

Subunit structurei

Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Interacts with GABPB2 (PubMed:16412436). Interacts (via carboxy-terminus) with TAX1BP3 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GABPB1Q065473EBI-8014984,EBI-618165From a different organism.

Protein-protein interaction databases

IntActiQ8CGM1. 1 interactor.
MINTiMINT-1504097.
STRINGi10090.ENSMUSP00000101638.

Structurei

3D structure databases

ProteinModelPortaliQ8CGM1.
SMRiQ8CGM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 353TSP type-1 1PROSITE-ProRule annotationAdd BLAST54
Domaini355 – 408TSP type-1 2PROSITE-ProRule annotationAdd BLAST54
Domaini410 – 463TSP type-1 3PROSITE-ProRule annotationAdd BLAST54
Domaini466 – 519TSP type-1 4PROSITE-ProRule annotationAdd BLAST54
Domaini865 – 917GPSPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi117 – 122Poly-Glu6
Compositional biasi123 – 127Poly-Ala5
Compositional biasi180 – 183Poly-Asn4
Compositional biasi1309 – 1312Poly-Pro4
Compositional biasi1358 – 1361Poly-Gly4
Compositional biasi1419 – 1424Poly-Pro6

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 4 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEA0. Eukaryota.
ENOG4111FBM. LUCA.
GeneTreeiENSGT00850000132260.
HOVERGENiHBG004813.
InParanoidiQ8CGM1.
KOiK04597.
PhylomeDBiQ8CGM1.
TreeFamiTF331634.

Family and domain databases

InterProiIPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR008077. GPCR_2_brain_angio_inhib.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
PF00090. TSP_1. 4 hits.
[Graphical view]
PRINTSiPR01694. BAIPRECURSOR.
PR00249. GPCRSECRETIN.
SMARTiSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00209. TSP1. 4 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 4 hits.
PROSITEiPS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50092. TSP1. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CGM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTPACPLLLS VILSLRLATA FDPAPSACSA LASGVLYGAF SLQDLFPTIA
60 70 80 90 100
SGCSWTLENP DPTKYSLYLR FNRQEQVCTH FAPRLLPLDH YLVNFTCLRP
110 120 130 140 150
GPEEATARAE SEVGRPEEEE EEAAAAASGL ELCGGSGPFT FLHFDKNFVQ
160 170 180 190 200
LCLSAEPSEA PRLLAPAALA FRFVEVLLIN NNNSSQFTCG VLCRWSEECG
210 220 230 240 250
RAAGRACGFA QPGCSCPGEA GANPATTTSP GPPVAHTLSN ALVPGGPAPP
260 270 280 290 300
AEADLHSGSS NDLFTTEMRY GEEPEEEPKV KTQWPRSADE PGLYMAQTGD
310 320 330 340 350
PAAEEWSPWS VCSLTCGQGL QVRTRSCVSS PYGTLCSGPL RETRPCNNSA
360 370 380 390 400
TCPVHGVWEE WGSWSLCSRS CGRGSRSRMR TCVPPQHGGK ACEGPELQTK
410 420 430 440 450
LCSMAACPVE GQWLEWGPWG PCSSSCANGT QQRSRKCSVA GPAWATCAGA
460 470 480 490 500
LTDTRECSNL DCPATDGKWG PWNAWSLCSK TCDTGWQRRF RMCQASGTQG
510 520 530 540 550
YPCEGTGEEV KPCSEKRCPA FHEMCRDEYV MLMTWKRAAA GEIIYNKCPP
560 570 580 590 600
NASGSASRRC LLSAQGVAYW GLPSFARCIS HEYRYLYLSL REHLAKGQRM
610 620 630 640 650
LAGEGMSQVV RSLQELLARR TYYSGDLLFS VDILRNVTDT FKRATYVPSA
660 670 680 690 700
DDVQRFFQVV SFMVDSENKD KWDDAQQVSP GSVHLLRVVE DFIHLVGDAL
710 720 730 740 750
KAFQSSLIVT DNLVISIQRE PISAVSSDIT FPMRGRRGMK DWVRHSEDRL
760 770 780 790 800
FLPKEVLSLS SPGKPATPGA ATAGSPGRGR GPGTVPPGPG HAHQRLLPAD
810 820 830 840 850
PEESSSYFVI GAVLYRTLGL ILPPPRPPLA VTSRVMTVTV RPPTQPPAEP
860 870 880 890 900
LITVELSYII NGTTDPHCAS WDYSRADTNS GDWNTESCQT LETQAAHTRC
910 920 930 940 950
QCQHLSTFAV LAQPPKDLTL ELAGAPSVPL VIGCAVSCMA LLTLLAIYAA
960 970 980 990 1000
FWRFIKSERS IILLNFCLSI LASNILILVG QSRVLSKGVC TMTAAFLHFF
1010 1020 1030 1040 1050
FLSSFCWVLT EAWQSYLAVI GRMRTRLVRK RFLCLGWGLP ALVVAVSVGF
1060 1070 1080 1090 1100
TRTKGYGTSS YCWLSLEGGL LYAFVGPAAV IVLVNMLIGI IVFNKLMARD
1110 1120 1130 1140 1150
GVSDKSKKQR AGSERCPWAS LLLPCSACGA VPSPLLSSAS ARNAMASLWS
1160 1170 1180 1190 1200
SCVVLPLLAL TWMSAVLAMT DRRSVLFQAL FAVFNSAQGF VITAVHCFLR
1210 1220 1230 1240 1250
REVQDVVKCQ MGVCRADESE DSPDSCKNGQ LQILSDFEKD VDLACQTVLF
1260 1270 1280 1290 1300
KEVNTCNPST ITGTLSRLSL DEDEEPKSCL VGPEGGLSFS PLPGNILVPM
1310 1320 1330 1340 1350
AASPGLGEPP PPQETNPVYM CGEGGLRQLD LTWIRQSEPG SEGDYMVLPR
1360 1370 1380 1390 1400
RTLSLQPGGG GTAGEEAPRA RPEGTPRRAA KTVAHTEGYP SFLSVEHSGL
1410 1420 1430 1440 1450
GLGPAYGSLQ NPYGMTFQPP PPTPSARQVP EPGERSRTMP RTVPGSTMKL
1460 1470 1480 1490 1500
GSLERKKLRY SDLDFEKVMH TRKRHSELYH ELNQKFHTFD RYRSQSSAKE
1510 1520 1530 1540 1550
KPSPPGGRPG LSQHRRHQSW STFKSMTLGS LPPKPRERLA LHRTAAWEPT
1560
EPPDGDFQTE V
Length:1,561
Mass (Da):169,863
Last modified:July 11, 2006 - v2
Checksum:i3B3EB731F619CBA3
GO
Isoform 2 (identifier: Q8CGM1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     299-353: Missing.

Show »
Length:1,506
Mass (Da):164,063
Checksum:i6164F02425F64DC1
GO
Isoform 3 (identifier: Q8CGM1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1113-1145: SERCPWASLLLPCSACGAVPSPLLSSASARNAM → RLSWNLWGYGSQLCLFPKLPR

Show »
Length:1,549
Mass (Da):169,051
Checksum:i7051D25C930EA7C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116P → L in AAN86965 (PubMed:12218411).Curated1
Sequence conflicti122Missing in AAN86965 (PubMed:12218411).Curated1
Sequence conflicti386Q → K in AAN86965 (PubMed:12218411).Curated1
Sequence conflicti526R → G in BAE34635 (PubMed:16141072).Curated1
Sequence conflicti674D → G in BAE34635 (PubMed:16141072).Curated1
Sequence conflicti1179A → V in BAE34635 (PubMed:16141072).Curated1
Sequence conflicti1316N → D in BAE25937 (PubMed:16141072).Curated1
Sequence conflicti1467Missing in BAE25937 (PubMed:16141072).Curated1
Sequence conflicti1467Missing in BAE25805 (PubMed:16141072).Curated1
Sequence conflicti1550T → P in AAN86965 (PubMed:12218411).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019762299 – 353Missing in isoform 2. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_0197631113 – 1145SERCP…ARNAM → RLSWNLWGYGSQLCLFPKLP R in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY168407 mRNA. Translation: AAN86965.1.
AK144268 mRNA. Translation: BAE25805.1.
AK144559 mRNA. Translation: BAE25937.1.
AK158735 mRNA. Translation: BAE34635.1.
AL626774 Genomic DNA. Translation: CAM17272.1.
AL626774 Genomic DNA. Translation: CAM17273.1.
AL626774 Genomic DNA. Translation: CAQ52064.1.
BC056926 mRNA. Translation: AAH56926.1.
CCDSiCCDS51309.1. [Q8CGM1-3]
CCDS57300.1. [Q8CGM1-2]
CCDS80164.1. [Q8CGM1-1]
RefSeqiNP_001186625.1. NM_001199696.1. [Q8CGM1-2]
NP_001277643.1. NM_001290714.1.
NP_001277644.1. NM_001290715.1. [Q8CGM1-1]
NP_775094.2. NM_173071.3. [Q8CGM1-3]
UniGeneiMm.262241.

Genome annotation databases

EnsembliENSMUST00000106015; ENSMUSP00000101636; ENSMUSG00000028782. [Q8CGM1-1]
ENSMUST00000106017; ENSMUSP00000101638; ENSMUSG00000028782. [Q8CGM1-3]
ENSMUST00000121049; ENSMUSP00000112869; ENSMUSG00000028782. [Q8CGM1-2]
GeneIDi230775.
KEGGimmu:230775.
UCSCiuc008uym.3. mouse. [Q8CGM1-3]
uc008uyn.3. mouse. [Q8CGM1-2]
uc008uyp.1. mouse. [Q8CGM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY168407 mRNA. Translation: AAN86965.1.
AK144268 mRNA. Translation: BAE25805.1.
AK144559 mRNA. Translation: BAE25937.1.
AK158735 mRNA. Translation: BAE34635.1.
AL626774 Genomic DNA. Translation: CAM17272.1.
AL626774 Genomic DNA. Translation: CAM17273.1.
AL626774 Genomic DNA. Translation: CAQ52064.1.
BC056926 mRNA. Translation: AAH56926.1.
CCDSiCCDS51309.1. [Q8CGM1-3]
CCDS57300.1. [Q8CGM1-2]
CCDS80164.1. [Q8CGM1-1]
RefSeqiNP_001186625.1. NM_001199696.1. [Q8CGM1-2]
NP_001277643.1. NM_001290714.1.
NP_001277644.1. NM_001290715.1. [Q8CGM1-1]
NP_775094.2. NM_173071.3. [Q8CGM1-3]
UniGeneiMm.262241.

3D structure databases

ProteinModelPortaliQ8CGM1.
SMRiQ8CGM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CGM1. 1 interactor.
MINTiMINT-1504097.
STRINGi10090.ENSMUSP00000101638.

Protein family/group databases

MEROPSiP02.029.
GPCRDBiSearch...

PTM databases

iPTMnetiQ8CGM1.
PhosphoSitePlusiQ8CGM1.

Proteomic databases

PaxDbiQ8CGM1.
PRIDEiQ8CGM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106015; ENSMUSP00000101636; ENSMUSG00000028782. [Q8CGM1-1]
ENSMUST00000106017; ENSMUSP00000101638; ENSMUSG00000028782. [Q8CGM1-3]
ENSMUST00000121049; ENSMUSP00000112869; ENSMUSG00000028782. [Q8CGM1-2]
GeneIDi230775.
KEGGimmu:230775.
UCSCiuc008uym.3. mouse. [Q8CGM1-3]
uc008uyn.3. mouse. [Q8CGM1-2]
uc008uyp.1. mouse. [Q8CGM1-1]

Organism-specific databases

CTDi576.
MGIiMGI:2451244. Adgrb2.

Phylogenomic databases

eggNOGiENOG410IEA0. Eukaryota.
ENOG4111FBM. LUCA.
GeneTreeiENSGT00850000132260.
HOVERGENiHBG004813.
InParanoidiQ8CGM1.
KOiK04597.
PhylomeDBiQ8CGM1.
TreeFamiTF331634.

Miscellaneous databases

ChiTaRSiBai2. mouse.
PROiQ8CGM1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028782.
CleanExiMM_BAI2.
ExpressionAtlasiQ8CGM1. baseline and differential.
GenevisibleiQ8CGM1. MM.

Family and domain databases

InterProiIPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR008077. GPCR_2_brain_angio_inhib.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
PF00090. TSP_1. 4 hits.
[Graphical view]
PRINTSiPR01694. BAIPRECURSOR.
PR00249. GPCRSECRETIN.
SMARTiSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00209. TSP1. 4 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 4 hits.
PROSITEiPS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50092. TSP1. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRB2_MOUSE
AccessioniPrimary (citable) accession number: Q8CGM1
Secondary accession number(s): B1ASB7
, B1ASB8, B2FDE3, Q3TYC8, Q3UN11, Q3UNE2, Q6PGN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.