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Protein

Adhesion G protein-coupled receptor B2

Gene

Adgrb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Orphan G-protein coupled receptor involved in cell adhesion and probably in cell-cell interactions. Activates NFAT-signaling pathway, a transcription factor, via the G-protein GNAZ. Involved in angiogenesis inhibition (PubMed:12218411).By similarity1 Publication

Enzyme regulationi

Receptor activity is regulated by proteolytic processing. The long N-terminal has a an inhibitory effect on the constitutive signaling activity. Removal of the N-terminal region induces an increase of the receptor activity.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Protein family/group databases

MEROPSiP02.029

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor B2
Alternative name(s):
Brain-specific angiogenesis inhibitor 2
Gene namesi
Name:Adgrb2
Synonyms:Bai2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2451244 Adgrb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 930ExtracellularCuratedAdd BLAST910
Transmembranei931 – 951Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini952 – 959CytoplasmicCurated8
Transmembranei960 – 980Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini981 – 988ExtracellularCurated8
Transmembranei989 – 1009Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini1010 – 1030CytoplasmicCuratedAdd BLAST21
Transmembranei1031 – 1051Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini1052 – 1072ExtracellularCuratedAdd BLAST21
Transmembranei1073 – 1093Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini1094 – 1115CytoplasmicCuratedAdd BLAST22
Transmembranei1116 – 1136Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1137 – 1147ExtracellularCuratedAdd BLAST11
Transmembranei1148 – 1168Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1169 – 1561CytoplasmicCuratedAdd BLAST393

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice show significant resistance to depression after repeated stress in the social defeat test. Additionally, hippocampal cell proliferation in deficient mice is increased.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000024561321 – 1561Adhesion G protein-coupled receptor B2Add BLAST1541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi182N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi183N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi312 ↔ 346PROSITE-ProRule annotation
Disulfide bondi316 ↔ 352PROSITE-ProRule annotation
Disulfide bondi327 ↔ 336PROSITE-ProRule annotation
Glycosylationi347N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi367 ↔ 402PROSITE-ProRule annotation
Disulfide bondi371 ↔ 407PROSITE-ProRule annotation
Disulfide bondi382 ↔ 392PROSITE-ProRule annotation
Disulfide bondi422 ↔ 457PROSITE-ProRule annotation
Disulfide bondi426 ↔ 462PROSITE-ProRule annotation
Glycosylationi428N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi437 ↔ 447PROSITE-ProRule annotation
Disulfide bondi478 ↔ 513PROSITE-ProRule annotation
Disulfide bondi482 ↔ 518PROSITE-ProRule annotation
Disulfide bondi493 ↔ 503PROSITE-ProRule annotation
Disulfide bondi525 ↔ 560PROSITE-ProRule annotation
Disulfide bondi548 ↔ 578PROSITE-ProRule annotation
Glycosylationi551N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi636N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi861N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi868 ↔ 900PROSITE-ProRule annotation
Disulfide bondi888 ↔ 902PROSITE-ProRule annotation
Modified residuei1345PhosphotyrosineCombined sources1

Post-translational modificationi

Glycosylated.By similarity
Proteolytic processes at the GPS domain; this cleavage modulates receptor activity. Additionally, furin is involved in the cleavage at another site, in the middle of the extracellular domain, generating a soluble fragment.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei287 – 288Cleavage; by furinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8CGM1
PRIDEiQ8CGM1

PTM databases

iPTMnetiQ8CGM1
PhosphoSitePlusiQ8CGM1

Expressioni

Tissue specificityi

Specifically expressed in the brain. The peak level in the brain is observed 10 days after birth.1 Publication

Developmental stagei

Ubiquitous in embryonic tissues, but expression is acutely down-regulated after birth, except in the brain, to a level that is maintained throughout adulthood.1 Publication

Inductioni

Down-regulated after hypoxia.

Gene expression databases

BgeeiENSMUSG00000028782
CleanExiMM_BAI2
ExpressionAtlasiQ8CGM1 baseline and differential
GenevisibleiQ8CGM1 MM

Interactioni

Subunit structurei

Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Interacts with GABPB2 (PubMed:16412436). Interacts (via carboxy-terminus) with TAX1BP3. Interacts with GNAZ (By similarity). Interacts with SH3GL2 (PubMed:28891236).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GABPB1Q065473EBI-8014984,EBI-618165From Homo sapiens.

Protein-protein interaction databases

BioGridi231022, 2 interactors
IntActiQ8CGM1, 3 interactors
MINTiQ8CGM1
STRINGi10090.ENSMUSP00000101638

Structurei

3D structure databases

ProteinModelPortaliQ8CGM1
SMRiQ8CGM1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 353TSP type-1 1PROSITE-ProRule annotationAdd BLAST54
Domaini355 – 408TSP type-1 2PROSITE-ProRule annotationAdd BLAST54
Domaini410 – 463TSP type-1 3PROSITE-ProRule annotationAdd BLAST54
Domaini466 – 519TSP type-1 4PROSITE-ProRule annotationAdd BLAST54
Domaini865 – 917GPSPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi117 – 122Poly-Glu6
Compositional biasi123 – 127Poly-Ala5
Compositional biasi180 – 183Poly-Asn4
Compositional biasi1309 – 1312Poly-Pro4
Compositional biasi1358 – 1361Poly-Gly4
Compositional biasi1419 – 1424Poly-Pro6

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEA0 Eukaryota
ENOG4111FBM LUCA
GeneTreeiENSGT00910000144013
HOVERGENiHBG004813
InParanoidiQ8CGM1
KOiK04597
PhylomeDBiQ8CGM1
TreeFamiTF331634

Family and domain databases

Gene3Di2.20.100.10, 4 hits
4.10.1240.10, 1 hit
InterProiView protein in InterPro
IPR032471 GAIN_dom_N
IPR017981 GPCR_2-like
IPR008077 GPCR_2_brain_angio_inhib
IPR036445 GPCR_2_extracell_dom_sf
IPR001879 GPCR_2_extracellular_dom
IPR000832 GPCR_2_secretin-like
IPR000203 GPS
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF00002 7tm_2, 1 hit
PF16489 GAIN, 1 hit
PF01825 GPS, 1 hit
PF00090 TSP_1, 4 hits
PRINTSiPR01694 BAIPRECURSOR
PR00249 GPCRSECRETIN
SMARTiView protein in SMART
SM00303 GPS, 1 hit
SM00008 HormR, 1 hit
SM00209 TSP1, 4 hits
SUPFAMiSSF82895 SSF82895, 4 hits
PROSITEiView protein in PROSITE
PS50227 G_PROTEIN_RECEP_F2_3, 1 hit
PS50261 G_PROTEIN_RECEP_F2_4, 1 hit
PS50221 GPS, 1 hit
PS50092 TSP1, 4 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CGM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTPACPLLLS VILSLRLATA FDPAPSACSA LASGVLYGAF SLQDLFPTIA
60 70 80 90 100
SGCSWTLENP DPTKYSLYLR FNRQEQVCTH FAPRLLPLDH YLVNFTCLRP
110 120 130 140 150
GPEEATARAE SEVGRPEEEE EEAAAAASGL ELCGGSGPFT FLHFDKNFVQ
160 170 180 190 200
LCLSAEPSEA PRLLAPAALA FRFVEVLLIN NNNSSQFTCG VLCRWSEECG
210 220 230 240 250
RAAGRACGFA QPGCSCPGEA GANPATTTSP GPPVAHTLSN ALVPGGPAPP
260 270 280 290 300
AEADLHSGSS NDLFTTEMRY GEEPEEEPKV KTQWPRSADE PGLYMAQTGD
310 320 330 340 350
PAAEEWSPWS VCSLTCGQGL QVRTRSCVSS PYGTLCSGPL RETRPCNNSA
360 370 380 390 400
TCPVHGVWEE WGSWSLCSRS CGRGSRSRMR TCVPPQHGGK ACEGPELQTK
410 420 430 440 450
LCSMAACPVE GQWLEWGPWG PCSSSCANGT QQRSRKCSVA GPAWATCAGA
460 470 480 490 500
LTDTRECSNL DCPATDGKWG PWNAWSLCSK TCDTGWQRRF RMCQASGTQG
510 520 530 540 550
YPCEGTGEEV KPCSEKRCPA FHEMCRDEYV MLMTWKRAAA GEIIYNKCPP
560 570 580 590 600
NASGSASRRC LLSAQGVAYW GLPSFARCIS HEYRYLYLSL REHLAKGQRM
610 620 630 640 650
LAGEGMSQVV RSLQELLARR TYYSGDLLFS VDILRNVTDT FKRATYVPSA
660 670 680 690 700
DDVQRFFQVV SFMVDSENKD KWDDAQQVSP GSVHLLRVVE DFIHLVGDAL
710 720 730 740 750
KAFQSSLIVT DNLVISIQRE PISAVSSDIT FPMRGRRGMK DWVRHSEDRL
760 770 780 790 800
FLPKEVLSLS SPGKPATPGA ATAGSPGRGR GPGTVPPGPG HAHQRLLPAD
810 820 830 840 850
PEESSSYFVI GAVLYRTLGL ILPPPRPPLA VTSRVMTVTV RPPTQPPAEP
860 870 880 890 900
LITVELSYII NGTTDPHCAS WDYSRADTNS GDWNTESCQT LETQAAHTRC
910 920 930 940 950
QCQHLSTFAV LAQPPKDLTL ELAGAPSVPL VIGCAVSCMA LLTLLAIYAA
960 970 980 990 1000
FWRFIKSERS IILLNFCLSI LASNILILVG QSRVLSKGVC TMTAAFLHFF
1010 1020 1030 1040 1050
FLSSFCWVLT EAWQSYLAVI GRMRTRLVRK RFLCLGWGLP ALVVAVSVGF
1060 1070 1080 1090 1100
TRTKGYGTSS YCWLSLEGGL LYAFVGPAAV IVLVNMLIGI IVFNKLMARD
1110 1120 1130 1140 1150
GVSDKSKKQR AGSERCPWAS LLLPCSACGA VPSPLLSSAS ARNAMASLWS
1160 1170 1180 1190 1200
SCVVLPLLAL TWMSAVLAMT DRRSVLFQAL FAVFNSAQGF VITAVHCFLR
1210 1220 1230 1240 1250
REVQDVVKCQ MGVCRADESE DSPDSCKNGQ LQILSDFEKD VDLACQTVLF
1260 1270 1280 1290 1300
KEVNTCNPST ITGTLSRLSL DEDEEPKSCL VGPEGGLSFS PLPGNILVPM
1310 1320 1330 1340 1350
AASPGLGEPP PPQETNPVYM CGEGGLRQLD LTWIRQSEPG SEGDYMVLPR
1360 1370 1380 1390 1400
RTLSLQPGGG GTAGEEAPRA RPEGTPRRAA KTVAHTEGYP SFLSVEHSGL
1410 1420 1430 1440 1450
GLGPAYGSLQ NPYGMTFQPP PPTPSARQVP EPGERSRTMP RTVPGSTMKL
1460 1470 1480 1490 1500
GSLERKKLRY SDLDFEKVMH TRKRHSELYH ELNQKFHTFD RYRSQSSAKE
1510 1520 1530 1540 1550
KPSPPGGRPG LSQHRRHQSW STFKSMTLGS LPPKPRERLA LHRTAAWEPT
1560
EPPDGDFQTE V
Length:1,561
Mass (Da):169,863
Last modified:July 11, 2006 - v2
Checksum:i3B3EB731F619CBA3
GO
Isoform 2 (identifier: Q8CGM1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     299-353: Missing.

Show »
Length:1,506
Mass (Da):164,063
Checksum:i6164F02425F64DC1
GO
Isoform 3 (identifier: Q8CGM1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1113-1145: SERCPWASLLLPCSACGAVPSPLLSSASARNAM → RLSWNLWGYGSQLCLFPKLPR

Show »
Length:1,549
Mass (Da):169,051
Checksum:i7051D25C930EA7C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116P → L in AAN86965 (PubMed:12218411).Curated1
Sequence conflicti122Missing in AAN86965 (PubMed:12218411).Curated1
Sequence conflicti386Q → K in AAN86965 (PubMed:12218411).Curated1
Sequence conflicti526R → G in BAE34635 (PubMed:16141072).Curated1
Sequence conflicti674D → G in BAE34635 (PubMed:16141072).Curated1
Sequence conflicti1179A → V in BAE34635 (PubMed:16141072).Curated1
Sequence conflicti1316N → D in BAE25937 (PubMed:16141072).Curated1
Sequence conflicti1467Missing in BAE25937 (PubMed:16141072).Curated1
Sequence conflicti1467Missing in BAE25805 (PubMed:16141072).Curated1
Sequence conflicti1550T → P in AAN86965 (PubMed:12218411).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019762299 – 353Missing in isoform 2. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_0197631113 – 1145SERCP…ARNAM → RLSWNLWGYGSQLCLFPKLP R in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY168407 mRNA Translation: AAN86965.1
AK144268 mRNA Translation: BAE25805.1
AK144559 mRNA Translation: BAE25937.1
AK158735 mRNA Translation: BAE34635.1
AL626774 Genomic DNA No translation available.
BC056926 mRNA Translation: AAH56926.1
CCDSiCCDS51309.1 [Q8CGM1-3]
CCDS57300.1 [Q8CGM1-2]
CCDS80164.1 [Q8CGM1-1]
RefSeqiNP_001186625.1, NM_001199696.1 [Q8CGM1-2]
NP_001277643.1, NM_001290714.1
NP_001277644.1, NM_001290715.1 [Q8CGM1-1]
NP_775094.2, NM_173071.3 [Q8CGM1-3]
UniGeneiMm.262241

Genome annotation databases

EnsembliENSMUST00000106015; ENSMUSP00000101636; ENSMUSG00000028782 [Q8CGM1-1]
ENSMUST00000106017; ENSMUSP00000101638; ENSMUSG00000028782 [Q8CGM1-3]
ENSMUST00000121049; ENSMUSP00000112869; ENSMUSG00000028782 [Q8CGM1-2]
GeneIDi230775
KEGGimmu:230775
UCSCiuc008uym.3 mouse [Q8CGM1-3]
uc008uyn.3 mouse [Q8CGM1-2]
uc008uyp.1 mouse [Q8CGM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAGRB2_MOUSE
AccessioniPrimary (citable) accession number: Q8CGM1
Secondary accession number(s): B1ASB7
, B1ASB8, B2FDE3, Q3TYC8, Q3UN11, Q3UNE2, Q6PGN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: March 28, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families
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