Guanine nucleotide-binding protein G(olf) subunit alpha

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Q8CGK7 (GNAL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(olf) subunit alpha

Alternative name(s):
Adenylate cyclase-stimulating G alpha protein, olfactory type

Gene names

Name:

Gnal

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters By similarity.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Sequence similarities	Belongs to the G-alpha family. G(s) subfamily.

Ontologies

Keywords
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	activation of adenylate cyclase activity Inferred from direct assay PubMed 12488442. Source: MGI adenylate cyclase-activating dopamine receptor signaling pathway Inferred from genetic interaction PubMed 17194762. Source: MGI response to amphetamine Inferred from mutant phenotype PubMed 11404425. Source: MGI response to caffeine Inferred from mutant phenotype PubMed 11404425. Source: MGI sensory perception of smell Inferred from mutant phenotype PubMed 9459443. Source: MGI
Cellular_component	heterotrimeric G-protein complex Inferred from sequence alignment Ref.4. Source: MGI
Molecular_function	G-protein beta/gamma-subunit complex binding Inferred from Biological aspect of Ancestor. Source: RefGenome G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from sequence alignment Ref.4. Source: MGI adenylate cyclase activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 381

381

Guanine nucleotide-binding protein G(olf) subunit alpha

PRO_0000203733

Regions

Nucleotide binding

49 – 56

GTP By similarity

Nucleotide binding

185 – 191

GTP By similarity

Nucleotide binding

210 – 214

GTP By similarity

Nucleotide binding

279 – 282

GTP By similarity

Sites

Metal binding

Magnesium By similarity

Metal binding

191

Magnesium By similarity

Binding site

353

GTP; via amide nitrogen By similarity

Amino acid modifications

Lipidation

N-palmitoyl glycine By similarity

Lipidation

S-palmitoyl cysteine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8CGK7 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: F707E668EC279033
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FASTA

381

44,308

        10         20         30         40         50         60 
MGCLGNSSKT AEDQGVDEKE RREANKKIEK QLQKERLAYK ATHRLLLLGA GESGKSTIVK 

        70         80         90        100        110        120 
QMRILHVNGF NPEEKKQKIL DIRKNVKDAI VTIVSAMSTI IPPVPLANPE NQFRSDYIKS 

       130        140        150        160        170        180 
IAPITDFEYS QEFFDHVKKL WDDEGVKACF ERSNEYQLID CAQYFLERID SVSLVDYTPT 

       190        200        210        220        230        240 
DQDLLRCRVL TSGIFETRFQ VDKVNFHMFD VGGQRDERRK WIQCFNDVTA IIYVAACSSY 

       250        260        270        280        290        300 
NMVIREDNNT NRLRESLDLF ESIWNNRWLR TISIILFLNK QDMLAEKVLA GKSKIEDYFP 

       310        320        330        340        350        360 
EYANYTVPED ATPDAGEDPK VTRAKFFIRD LFLRISTATG DGKHYCYPHF TCAVDTENIR 

       370        380 
RVFNDCRDII QRMHLKQYEL L

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References

[1]	"The mouse G protein Golf alpha subunit full length coding sequence." Von Dannecker L.E.C., Malnic B. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 45-55, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[3]	"G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development." Williams C.J., Schultz R.M., Kopf G.S. Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 209-373. Strain: CF-1 / Harlan. Tissue: Brain.
[4]	"Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse." Strathmann M., Wilkie T.M., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 86:7407-7409(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 216-276. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY179169 mRNA. Translation: AAO03564.1. U38503 mRNA. Translation: AAB01734.1. M57635 mRNA. Translation: AAA63300.1.
IPI	IPI00229525.
PIR	A33833.
RefSeq	NP_034437.1. NM_010307.2.
UniGene	Mm.440746.
3D structure databases
ProteinModelPortal	Q8CGK7.
SMR	Q8CGK7. Positions 12-381.
ModBase	Search...
PTM databases
PhosphoSite	Q8CGK7.
Proteomic databases
PaxDb	Q8CGK7.
PRIDE	Q8CGK7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000076605; ENSMUSP00000075908; ENSMUSG00000024524.
GeneID	14680.
KEGG	mmu:14680.
UCSC	uc008flt.1. mouse.
Organism-specific databases
CTD	2774.
MGI	MGI:95774. Gnal.
Phylogenomic databases
eggNOG	NOG325092.
GeneTree	ENSGT00690000102066.
HOGENOM	HOG000038729.
HOVERGEN	HBG063184.
KO	K04633.
OMA	DYSQEFF.
Gene expression databases
ArrayExpress	Q8CGK7.
Bgee	Q8CGK7.
Genevestigator	Q8CGK7.
GermOnline	ENSMUSG00000024524. Mus musculus.
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR000367. Gprotein_alpha_S. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00443. GPROTEINAS.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	GNAL. mouse.
NextBio	286586.
SOURCE	Search...

Entry information

Entry name

GNAL_MOUSE

Accession

Primary (citable) accession number: Q8CGK7
Secondary accession number(s): Q61020, Q61589

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents