Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8CGK7 (GNAL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein G(olf) subunit alpha
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein, olfactory type
Gene names
Name:Gnal
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters By similarity.

Subunit structure

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Sequence similarities

Belongs to the G-alpha family. G(s) subfamily.

Ontologies

Keywords
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionTransducer
   PTMLipoprotein
Palmitate
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from sequence alignment Ref.4. Source: MGI

activation of adenylate cyclase activity

Inferred from direct assay PubMed 12488442. Source: MGI

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 2499043. Source: MGI

adenylate cyclase-activating dopamine receptor signaling pathway

Inferred from genetic interaction PubMed 17194762. Source: MGI

cAMP biosynthetic process

Inferred from Biological aspect of Ancestor. Source: GOC

response to amphetamine

Inferred from mutant phenotype PubMed 11404425. Source: MGI

response to caffeine

Inferred from mutant phenotype PubMed 11404425. Source: MGI

sensory perception of smell

Inferred from mutant phenotype PubMed 9459443. Source: MGI

   Cellular_componentextrinsic component of cytoplasmic side of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

heterotrimeric G-protein complex

Inferred from sequence alignment Ref.4. Source: MGI

   Molecular_functionG-protein beta/gamma-subunit complex binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

G-protein coupled receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from sequence alignment Ref.4. Source: MGI

adenylate cyclase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

signal transducer activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Guanine nucleotide-binding protein G(olf) subunit alpha
PRO_0000203733

Regions

Nucleotide binding49 – 568GTP By similarity
Nucleotide binding185 – 1917GTP By similarity
Nucleotide binding210 – 2145GTP By similarity
Nucleotide binding279 – 2824GTP By similarity

Sites

Metal binding561Magnesium By similarity
Metal binding1911Magnesium By similarity
Binding site3531GTP; via amide nitrogen By similarity

Amino acid modifications

Lipidation21N-palmitoyl glycine By similarity
Lipidation31S-palmitoyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CGK7 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: F707E668EC279033

FASTA38144,308
        10         20         30         40         50         60 
MGCLGNSSKT AEDQGVDEKE RREANKKIEK QLQKERLAYK ATHRLLLLGA GESGKSTIVK 

        70         80         90        100        110        120 
QMRILHVNGF NPEEKKQKIL DIRKNVKDAI VTIVSAMSTI IPPVPLANPE NQFRSDYIKS 

       130        140        150        160        170        180 
IAPITDFEYS QEFFDHVKKL WDDEGVKACF ERSNEYQLID CAQYFLERID SVSLVDYTPT 

       190        200        210        220        230        240 
DQDLLRCRVL TSGIFETRFQ VDKVNFHMFD VGGQRDERRK WIQCFNDVTA IIYVAACSSY 

       250        260        270        280        290        300 
NMVIREDNNT NRLRESLDLF ESIWNNRWLR TISIILFLNK QDMLAEKVLA GKSKIEDYFP 

       310        320        330        340        350        360 
EYANYTVPED ATPDAGEDPK VTRAKFFIRD LFLRISTATG DGKHYCYPHF TCAVDTENIR 

       370        380 
RVFNDCRDII QRMHLKQYEL L 

« Hide

References

[1]"The mouse G protein Golf alpha subunit full length coding sequence."
Von Dannecker L.E.C., Malnic B.
Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 45-55, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[3]"G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
Williams C.J., Schultz R.M., Kopf G.S.
Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 209-373.
Strain: CF-1 / Harlan.
Tissue: Brain.
[4]"Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse."
Strathmann M., Wilkie T.M., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 86:7407-7409(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 216-276.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY179169 mRNA. Translation: AAO03564.1.
U38503 mRNA. Translation: AAB01734.1.
M57635 mRNA. Translation: AAA63300.1.
CCDSCCDS29318.1.
PIRA33833.
RefSeqNP_034437.1. NM_010307.3.
UniGeneMm.440746.

3D structure databases

ProteinModelPortalQ8CGK7.
SMRQ8CGK7. Positions 12-381.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8CGK7.

Proteomic databases

MaxQBQ8CGK7.
PaxDbQ8CGK7.
PRIDEQ8CGK7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000076605; ENSMUSP00000075908; ENSMUSG00000024524.
GeneID14680.
KEGGmmu:14680.
UCSCuc008flt.1. mouse.

Organism-specific databases

CTD2774.
MGIMGI:95774. Gnal.

Phylogenomic databases

eggNOGNOG325092.
GeneTreeENSGT00690000102066.
HOGENOMHOG000038729.
HOVERGENHBG063184.
KOK04633.
OMADYSQEFF.
PhylomeDBQ8CGK7.

Gene expression databases

BgeeQ8CGK7.
GenevestigatorQ8CGK7.

Family and domain databases

Gene3D1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10218. PTHR10218. 1 hit.
PfamPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

ChiTaRSGNAL. mouse.
NextBio286586.
PROQ8CGK7.
SOURCESearch...

Entry information

Entry nameGNAL_MOUSE
AccessionPrimary (citable) accession number: Q8CGK7
Secondary accession number(s): Q61020, Q61589
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot