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Protein

Guanine nucleotide-binding protein G(olf) subunit alpha

Gene

Gnal

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561MagnesiumBy similarity
Metal bindingi191 – 1911MagnesiumBy similarity
Binding sitei353 – 3531GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 568GTPBy similarity
Nucleotide bindingi185 – 1917GTPBy similarity
Nucleotide bindingi210 – 2145GTPBy similarity
Nucleotide bindingi279 – 2824GTPBy similarity

GO - Molecular functioni

  1. G-protein beta/gamma-subunit complex binding Source: GO_Central
  2. G-protein coupled receptor binding Source: GO_Central
  3. GTPase activity Source: MGI
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. signal transducer activity Source: GO_Central

GO - Biological processi

  1. activation of adenylate cyclase activity Source: MGI
  2. adenylate cyclase-activating dopamine receptor signaling pathway Source: MGI
  3. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  4. G-protein coupled receptor signaling pathway Source: MGI
  5. GTP catabolic process Source: InterPro
  6. response to amphetamine Source: MGI
  7. response to caffeine Source: MGI
  8. sensory perception of smell Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_222824. Adenylate cyclase inhibitory pathway.
REACT_224974. Adenylate cyclase activating pathway.
REACT_252270. Olfactory Signaling Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(olf) subunit alpha
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein, olfactory type
Gene namesi
Name:Gnal
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:95774. Gnal.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. heterotrimeric G-protein complex Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Guanine nucleotide-binding protein G(olf) subunit alphaPRO_0000203733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-palmitoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

MaxQBiQ8CGK7.
PaxDbiQ8CGK7.
PRIDEiQ8CGK7.

PTM databases

PhosphoSiteiQ8CGK7.

Expressioni

Gene expression databases

BgeeiQ8CGK7.
ExpressionAtlasiQ8CGK7. baseline and differential.
GenevestigatoriQ8CGK7.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Structurei

3D structure databases

ProteinModelPortaliQ8CGK7.
SMRiQ8CGK7. Positions 12-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Curated

Phylogenomic databases

eggNOGiNOG325092.
GeneTreeiENSGT00770000120503.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiQ8CGK7.
KOiK04633.
OMAiCLGNSSK.
PhylomeDBiQ8CGK7.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8CGK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCLGNSSKT AEDQGVDEKE RREANKKIEK QLQKERLAYK ATHRLLLLGA
60 70 80 90 100
GESGKSTIVK QMRILHVNGF NPEEKKQKIL DIRKNVKDAI VTIVSAMSTI
110 120 130 140 150
IPPVPLANPE NQFRSDYIKS IAPITDFEYS QEFFDHVKKL WDDEGVKACF
160 170 180 190 200
ERSNEYQLID CAQYFLERID SVSLVDYTPT DQDLLRCRVL TSGIFETRFQ
210 220 230 240 250
VDKVNFHMFD VGGQRDERRK WIQCFNDVTA IIYVAACSSY NMVIREDNNT
260 270 280 290 300
NRLRESLDLF ESIWNNRWLR TISIILFLNK QDMLAEKVLA GKSKIEDYFP
310 320 330 340 350
EYANYTVPED ATPDAGEDPK VTRAKFFIRD LFLRISTATG DGKHYCYPHF
360 370 380
TCAVDTENIR RVFNDCRDII QRMHLKQYEL L
Length:381
Mass (Da):44,308
Last modified:March 1, 2003 - v1
Checksum:iF707E668EC279033
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY179169 mRNA. Translation: AAO03564.1.
U38503 mRNA. Translation: AAB01734.1.
M57635 mRNA. Translation: AAA63300.1.
CCDSiCCDS29318.1.
PIRiA33833.
RefSeqiNP_034437.1. NM_010307.3.
UniGeneiMm.440746.

Genome annotation databases

EnsembliENSMUST00000076605; ENSMUSP00000075908; ENSMUSG00000024524.
GeneIDi14680.
KEGGimmu:14680.
UCSCiuc008flt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY179169 mRNA. Translation: AAO03564.1.
U38503 mRNA. Translation: AAB01734.1.
M57635 mRNA. Translation: AAA63300.1.
CCDSiCCDS29318.1.
PIRiA33833.
RefSeqiNP_034437.1. NM_010307.3.
UniGeneiMm.440746.

3D structure databases

ProteinModelPortaliQ8CGK7.
SMRiQ8CGK7. Positions 12-381.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8CGK7.

Proteomic databases

MaxQBiQ8CGK7.
PaxDbiQ8CGK7.
PRIDEiQ8CGK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076605; ENSMUSP00000075908; ENSMUSG00000024524.
GeneIDi14680.
KEGGimmu:14680.
UCSCiuc008flt.1. mouse.

Organism-specific databases

CTDi2774.
MGIiMGI:95774. Gnal.

Phylogenomic databases

eggNOGiNOG325092.
GeneTreeiENSGT00770000120503.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiQ8CGK7.
KOiK04633.
OMAiCLGNSSK.
PhylomeDBiQ8CGK7.

Enzyme and pathway databases

ReactomeiREACT_222824. Adenylate cyclase inhibitory pathway.
REACT_224974. Adenylate cyclase activating pathway.
REACT_252270. Olfactory Signaling Pathway.

Miscellaneous databases

ChiTaRSiGnal. mouse.
NextBioi286586.
PROiQ8CGK7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CGK7.
ExpressionAtlasiQ8CGK7. baseline and differential.
GenevestigatoriQ8CGK7.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The mouse G protein Golf alpha subunit full length coding sequence."
    Von Dannecker L.E.C., Malnic B.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 45-55, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development."
    Williams C.J., Schultz R.M., Kopf G.S.
    Mol. Reprod. Dev. 44:315-323(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 209-373.
    Strain: CF-1 / Harlan.
    Tissue: Brain.
  4. "Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse."
    Strathmann M., Wilkie T.M., Simon M.I.
    Proc. Natl. Acad. Sci. U.S.A. 86:7407-7409(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 216-276.
    Tissue: Brain.

Entry informationi

Entry nameiGNAL_MOUSE
AccessioniPrimary (citable) accession number: Q8CGK7
Secondary accession number(s): Q61020, Q61589
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.