Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Interferon lambda-3

Gene

Ifnl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Antiviral defense

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon lambda-3
Short name:
IFN-lambda-3
Alternative name(s):
Interleukin-28B
Short name:
IL-28B
Gene namesi
Name:Ifnl3
Synonyms:Il28, Il28b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2450574. Ifnl3.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
  • intracellular Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 193174Interferon lambda-3PRO_0000015512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 132By similarity
Disulfide bondi69 ↔ 166By similarity
Disulfide bondi185 ↔ 192By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ8CGK6.

Expressioni

Gene expression databases

CleanExiMM_IL28B.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077473.

Structurei

3D structure databases

ProteinModelPortaliQ8CGK6.
SMRiQ8CGK6. Positions 35-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the lambda interferon family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40189.
GeneTreeiENSGT00390000014310.
HOVERGENiHBG052108.
InParanoidiQ8CGK6.
KOiK05447.
OMAiKVWENIN.
OrthoDBiEOG7F24V6.
TreeFamiTF336172.

Family and domain databases

InterProiIPR029177. INF_lambda.
[Graphical view]
PfamiPF15177. IL28A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CGK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLLLPLLL AAVLTRTQAD PVPRATRLPV EAKDCHIAQF KSLSPKELQA
60 70 80 90 100
FKKAKGAIEK RLLEKDMRCS SHLISRAWDL KQLQVQERPK ALQAEVALTL
110 120 130 140 150
KVWENINDSA LTTILGQPLH TLSHIHSQLQ TCTQLQATAE PKPPSRRLSR
160 170 180 190
WLHRLQEAQS KETPGCLEDS VTSNLFQLLL RDLKCVASGD QCV
Length:193
Mass (Da):21,664
Last modified:March 1, 2003 - v1
Checksum:i357A00AACAA9BC61
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561G → D in AAX58715 (PubMed:15914836).Curated
Sequence conflicti67 – 671M → V in AAX58715 (PubMed:15914836).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY184375 mRNA. Translation: AAN86128.1.
AY869696 mRNA. Translation: AAX58715.1.
CCDSiCCDS21047.1.
RefSeqiNP_796370.1. NM_177396.1.
UniGeneiMm.246799.

Genome annotation databases

EnsembliENSMUST00000078364; ENSMUSP00000077473; ENSMUSG00000060747.
GeneIDi338374.
KEGGimmu:338374.
UCSCiuc009fze.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY184375 mRNA. Translation: AAN86128.1.
AY869696 mRNA. Translation: AAX58715.1.
CCDSiCCDS21047.1.
RefSeqiNP_796370.1. NM_177396.1.
UniGeneiMm.246799.

3D structure databases

ProteinModelPortaliQ8CGK6.
SMRiQ8CGK6. Positions 35-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077473.

Proteomic databases

PRIDEiQ8CGK6.

Protocols and materials databases

DNASUi338374.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078364; ENSMUSP00000077473; ENSMUSG00000060747.
GeneIDi338374.
KEGGimmu:338374.
UCSCiuc009fze.1. mouse.

Organism-specific databases

CTDi282617.
MGIiMGI:2450574. Ifnl3.

Phylogenomic databases

eggNOGiNOG40189.
GeneTreeiENSGT00390000014310.
HOVERGENiHBG052108.
InParanoidiQ8CGK6.
KOiK05447.
OMAiKVWENIN.
OrthoDBiEOG7F24V6.
TreeFamiTF336172.

Miscellaneous databases

NextBioi400191.
PROiQ8CGK6.
SOURCEiSearch...

Gene expression databases

CleanExiMM_IL28B.

Family and domain databases

InterProiIPR029177. INF_lambda.
[Graphical view]
PfamiPF15177. IL28A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex."
    Kotenko S.V., Gallagher G., Baurin V.V., Lewis-Antes A., Shen M., Shah N.K., Langer J.A., Sheikh F., Dickensheets H., Donnelly R.P.
    Nat. Immunol. 4:69-77(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Murine interferon lambdas (type III interferons) exhibit potent antiviral activity in vivo in a poxvirus infection model."
    Bartlett N.W., Buttigieg K., Kotenko S.V., Smith G.L.
    J. Gen. Virol. 86:1589-1596(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: 129/Sv.
  3. "Characterization of the mouse IFN-lambda ligand-receptor system: IFN-lambdas exhibit antitumor activity against B16 melanoma."
    Lasfar A., Lewis-Antes A., Smirnov S.V., Anantha S., Abushahba W., Tian B., Reuhl K., Dickensheets H., Sheikh F., Donnelly R.P., Raveche E., Kotenko S.V.
    Cancer Res. 66:4468-4477(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "An important role for type III interferon (IFN-lambda/IL-28) in TLR-induced antiviral activity."
    Ank N., Iversen M.B., Bartholdy C., Staeheli P., Hartmann R., Jensen U.B., Dagnaes-Hansen F., Thomsen A.R., Chen Z., Haugen H., Klucher K., Paludan S.R.
    J. Immunol. 180:2474-2485(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Interferon-lambda: a new addition to an old family."
    Donnelly R.P., Kotenko S.V.
    J. Interferon Cytokine Res. 30:555-564(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. Cited for: FUNCTION.
  7. "Interferon-lambda in the context of viral infections: production, response and therapeutic implications."
    Hermant P., Michiels T.
    J. Innate Immun. 6:563-574(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiIFNL3_MOUSE
AccessioniPrimary (citable) accession number: Q8CGK6
Secondary accession number(s): Q4VK73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.