Q8CGK6 (IFNL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon lambda-3
Short name=IFN-lambda-3

Alternative name(s):
Interleukin-28B
Short name=IL-28B

Gene names

Name:	Ifnl3
Synonyms:	Il28, Il28b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Ref.2 Ref.3 Ref.4 Ref.5
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the lambda interferon family.

Ontologies

Keywords
Biological process	Antiviral defense
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from direct assay PubMed 16751416. Source: MGI
Cellular_component	extracellular space Inferred from direct assay PubMed 16751416. Source: MGI
Molecular_function	receptor binding Inferred from sequence alignment Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 193	174	Interferon lambda-3		PRO_0000015512
Amino acid modifications
	Disulfide bond	35 ↔ 132		By similarity
	Disulfide bond	69 ↔ 166		By similarity
	Disulfide bond	185 ↔ 192		By similarity
Experimental info
	Sequence conflict	56	1	G → D in AAX58715. Ref.2
	Sequence conflict	67	1	M → V in AAX58715. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8CGK6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 357A00AACAA9BC61	FASTA	193	21,664
	10 20 30 40 50 60 MLLLLLPLLL AAVLTRTQAD PVPRATRLPV EAKDCHIAQF KSLSPKELQA FKKAKGAIEK 70 80 90 100 110 120 RLLEKDMRCS SHLISRAWDL KQLQVQERPK ALQAEVALTL KVWENINDSA LTTILGQPLH 130 140 150 160 170 180 TLSHIHSQLQ TCTQLQATAE PKPPSRRLSR WLHRLQEAQS KETPGCLEDS VTSNLFQLLL 190 RDLKCVASGD QCV

References

[1]	"IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex." Kotenko S.V., Gallagher G., Baurin V.V., Lewis-Antes A., Shen M., Shah N.K., Langer J.A., Sheikh F., Dickensheets H., Donnelly R.P. Nat. Immunol. 4:69-77(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	"Murine interferon lambdas (type III interferons) exhibit potent antiviral activity in vivo in a poxvirus infection model." Bartlett N.W., Buttigieg K., Kotenko S.V., Smith G.L. J. Gen. Virol. 86:1589-1596(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: 129/Sv.
[3]	"Characterization of the mouse IFN-lambda ligand-receptor system: IFN-lambdas exhibit antitumor activity against B16 melanoma." Lasfar A., Lewis-Antes A., Smirnov S.V., Anantha S., Abushahba W., Tian B., Reuhl K., Dickensheets H., Sheikh F., Donnelly R.P., Raveche E., Kotenko S.V. Cancer Res. 66:4468-4477(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"An important role for type III interferon (IFN-lambda/IL-28) in TLR-induced antiviral activity." Ank N., Iversen M.B., Bartholdy C., Staeheli P., Hartmann R., Jensen U.B., Dagnaes-Hansen F., Thomsen A.R., Chen Z., Haugen H., Klucher K., Paludan S.R. J. Immunol. 180:2474-2485(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"IFN-lambda determines the intestinal epithelial antiviral host defense." Pott J., Mahlakoiv T., Mordstein M., Duerr C.U., Michiels T., Stockinger S., Staeheli P., Hornef M.W. Proc. Natl. Acad. Sci. U.S.A. 108:7944-7949(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY184375 mRNA. Translation: AAN86128.1. AY869696 mRNA. Translation: AAX58715.1.
CCDS	CCDS21047.1.
RefSeq	NP_796370.1. NM_177396.1.
UniGene	Mm.246799.
3D structure databases
ProteinModelPortal	Q8CGK6.
SMR	Q8CGK6. Positions 35-193.
ModBase	Search...
MobiDB	Search...
Proteomic databases
PRIDE	Q8CGK6.
Protocols and materials databases
DNASU	338374.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000078364; ENSMUSP00000077473; ENSMUSG00000060747.
GeneID	338374.
KEGG	mmu:338374.
UCSC	uc009fze.1. mouse.
Organism-specific databases
CTD	282617.
MGI	MGI:2450574. Ifnl3.
Phylogenomic databases
eggNOG	NOG40189.
GeneTree	ENSGT00390000014310.
HOVERGEN	HBG052108.
InParanoid	Q8CGK6.
KO	K05447.
OMA	AWERPVA.
OrthoDB	EOG7F24V6.
TreeFam	TF336172.
Gene expression databases
CleanEx	MM_IL28B.
Genevestigator	Q8CGK6.
Family and domain databases
InterPro	IPR029177. INF_lambda. [Graphical view]
Pfam	PF15177. IL28A. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	400191.
PRO	Q8CGK6.
SOURCE	Search...

Entry information

Entry name

IFNL3_MOUSE

Accession

Primary (citable) accession number: Q8CGK6
Secondary accession number(s): Q4VK73

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	March 1, 2003
Last modified:	July 9, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program