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Protein

Regulator of G-protein signaling 12

Gene

Rgs12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 12
Short name:
RGS12
Gene namesi
Name:Rgs12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1918979. Rgs12.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cell projectiondendrite By similarity
  • Cell junctionsynapse By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13811381Regulator of G-protein signaling 12PRO_0000408474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei171 – 1711PhosphoserineCombined sources
Modified residuei194 – 1941PhosphoserineCombined sources
Modified residuei661 – 6611PhosphoserineCombined sources
Modified residuei671 – 6711PhosphoserineCombined sources
Modified residuei850 – 8501PhosphoserineCombined sources
Modified residuei879 – 8791PhosphoserineCombined sources
Modified residuei943 – 9431PhosphoserineCombined sources
Cross-linki1115 – 1115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CGE9.
MaxQBiQ8CGE9.
PaxDbiQ8CGE9.
PRIDEiQ8CGE9.

PTM databases

iPTMnetiQ8CGE9.
PhosphoSiteiQ8CGE9.

Expressioni

Tissue specificityi

Expressed in brain.1 Publication

Developmental stagei

Expressed in germinal vesicle oocyte, metaphase II oocyte and blastocyst (at protein level). Expressed in oocyte.1 Publication

Gene expression databases

BgeeiQ8CGE9.
ExpressionAtlasiQ8CGE9. baseline and differential.
GenevisibleiQ8CGE9. MM.

Interactioni

Subunit structurei

Interacts with GNAI1, GNAI2 and GNAI3; the interactions are GDP-dependent.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BRAFP150562EBI-7340552,EBI-365980From a different organism.
Map2k2Q639323EBI-7340552,EBI-397724

Protein-protein interaction databases

IntActiQ8CGE9. 7 interactions.
MINTiMINT-4115792.
STRINGi10090.ENSMUSP00000030984.

Structurei

3D structure databases

ProteinModelPortaliQ8CGE9.
SMRiQ8CGE9. Positions 17-97, 705-852.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 9878PDZPROSITE-ProRule annotationAdd
BLAST
Domaini223 – 390168PIDPROSITE-ProRule annotationAdd
BLAST
Domaini715 – 832118RGSPROSITE-ProRule annotationAdd
BLAST
Domaini962 – 103271RBD 1PROSITE-ProRule annotationAdd
BLAST
Domaini1034 – 110471RBD 2PROSITE-ProRule annotationAdd
BLAST
Domaini1187 – 120923GoLocoPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1362 – 13676Poly-Pro

Domaini

The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.By similarity

Sequence similaritiesi

Contains 1 GoLoco domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation
Contains 2 RBD (Ras-binding) domains.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000049112.
HOVERGENiHBG063299.
InParanoidiQ8CGE9.
KOiK16449.
OrthoDBiEOG7XDBF0.
TreeFamiTF328814.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR003109. GoLoco_motif.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR003116. RBD_dom.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02188. GoLoco. 1 hit.
PF00595. PDZ. 1 hit.
PF02196. RBD. 2 hits.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00390. GoLoco. 1 hit.
SM00228. PDZ. 1 hit.
SM00462. PTB. 1 hit.
SM00455. RBD. 2 hits.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50877. GOLOCO. 1 hit.
PS50106. PDZ. 1 hit.
PS01179. PID. 1 hit.
PS50898. RBD. 2 hits.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CGE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRAGEPGKR QPGPAPPRVR SVEVARGRAG YGFTLSGQAP CVLSCVMRGS
60 70 80 90 100
PADFVGLRAG DQILAINEIN VKKASHEDVV KLIGKCSGVL HMVIAEGTSH
110 120 130 140 150
VESCSSDEEG GLYEGKGWLR PKLDSKALGI NRAERVVEEV QSGGIFNMIF
160 170 180 190 200
ESSSLCASGP EPLKLKQRSL SESAALRLDA GQAGLCAPHP SMLSKEDISK
210 220 230 240 250
VINDDSVFTV GLDSHDDFGL DASILNVAMV VGYLGSIELP STSSNLEHDS
260 270 280 290 300
LQAIRGCMRR LRAEQKIHSL VTMKVMHDCV QLVTDRAGVV AEYPAEKLAF
310 320 330 340 350
SAVCPDDRRF FGLVTMQTND DGGLAQEDEG ALRTSCHVFM VDPDLFHHKI
360 370 380 390 400
HQGIARRFGF ACTADPDTSG CLEFPASSLP VLQFISVLYR DMGELIEGVR
410 420 430 440 450
ARAFLDGDAD AHQNNSTSSN SDSGIGNFNQ EEKSNRVLVV DLGGGSSRHG
460 470 480 490 500
QGSSPGWESG GGRGSQPWSA PWNGAFCHDS EAGSPLETSP NTDRFWDLTK
510 520 530 540 550
HSGPVSHMEV PPATLRSSIP PSKRGAAGSS CGFNQRWLPV HVLQEWQCGH
560 570 580 590 600
ASDQESYTDS TDGWSSVNCG TLPPPMSKIP ADRYRVEGSF AQAPLSTQKR
610 620 630 640 650
DWSRKAFGMQ NLFGPHRNVR KTKEDKKSSK LGRGVALAQT SQRTSARRSF
660 670 680 690 700
GRSRRFSITR SLDDLESATV SDGELTGADL KDCISNNSLS SNASLPSVQS
710 720 730 740 750
CRRLRERRVA SWAVSFERLL QDPVGVRYFS DFLRKEFSEE NILFWQACEC
760 770 780 790 800
FSHVPAHDKK ELSYRAREIF SKFLCSKATT PVNIDSQAQL ADDILNAPHP
810 820 830 840 850
DMFKEQQLQI FNLMKFDSYT RFLKSQLYQE CVLAEVEGRT LPDSQQVPSS
860 870 880 890 900
PASKHSISSD HSNVSTPKKL SGKSKSGRSL NEDVGEEDSE KKRRGAFFSW
910 920 930 940 950
SRSRSTGRSQ KKKDHGDHAH DAPHANGGLC RRESQGSVSS AGSLDLSEAC
960 970 980 990 1000
RTSALEKDKA AKHCCVHLPD GTSCVVAVKS GFSIKEILSG LCERHGINGA
1010 1020 1030 1040 1050
AVDLFLVGGD KPLVLHQDSS ILATRDLRLE KRTLFRLDLV PINRSVGLKA
1060 1070 1080 1090 1100
KPTKPVTEVL RPVVAKYGLD LGSLLVRLSG EKEPLDLGAP ISSLDGQRVI
1110 1120 1130 1140 1150
LEERDPSRGK VSTDKQKGAP VKQNSAVNSS PRNHLAMGEE RTLGKSNSIK
1160 1170 1180 1190 1200
IRGENGKSAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN
1210 1220 1230 1240 1250
RADDQRGLLR KEDLVLPEFL RLPAGSSELA LSSPPPVKGY SKRAVTGHGQ
1260 1270 1280 1290 1300
EGAAQTEESY SDSPATSPAS AQSPCSAYSP GSAHSPGSAH STPGPPGTTQ
1310 1320 1330 1340 1350
PGEKPTKPSC VSMVQEGTTQ AWRRLSPEME AGGIQTVEDE QVADLTLMGE
1360 1370 1380
GDISSPNSTL LPPPPTPQDT PGPPRPGTSR F
Length:1,381
Mass (Da):149,637
Last modified:July 27, 2011 - v2
Checksum:i8897CD1D249B66C6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti536 – 5361R → K in AAH40396 (PubMed:15489334).Curated
Sequence conflicti1135 – 11351L → S in AAH40396 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126447 Genomic DNA. No translation available.
AC133204 Genomic DNA. No translation available.
BC040396 mRNA. Translation: AAH40396.1.
CCDSiCCDS19222.1.
RefSeqiNP_775578.2. NM_173402.2.
UniGeneiMm.196208.

Genome annotation databases

EnsembliENSMUST00000030984; ENSMUSP00000030984; ENSMUSG00000029101.
GeneIDi71729.
KEGGimmu:71729.
UCSCiuc008xdg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126447 Genomic DNA. No translation available.
AC133204 Genomic DNA. No translation available.
BC040396 mRNA. Translation: AAH40396.1.
CCDSiCCDS19222.1.
RefSeqiNP_775578.2. NM_173402.2.
UniGeneiMm.196208.

3D structure databases

ProteinModelPortaliQ8CGE9.
SMRiQ8CGE9. Positions 17-97, 705-852.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CGE9. 7 interactions.
MINTiMINT-4115792.
STRINGi10090.ENSMUSP00000030984.

PTM databases

iPTMnetiQ8CGE9.
PhosphoSiteiQ8CGE9.

Proteomic databases

EPDiQ8CGE9.
MaxQBiQ8CGE9.
PaxDbiQ8CGE9.
PRIDEiQ8CGE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030984; ENSMUSP00000030984; ENSMUSG00000029101.
GeneIDi71729.
KEGGimmu:71729.
UCSCiuc008xdg.2. mouse.

Organism-specific databases

CTDi6002.
MGIiMGI:1918979. Rgs12.

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000119142.
HOGENOMiHOG000049112.
HOVERGENiHBG063299.
InParanoidiQ8CGE9.
KOiK16449.
OrthoDBiEOG7XDBF0.
TreeFamiTF328814.

Miscellaneous databases

PROiQ8CGE9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CGE9.
ExpressionAtlasiQ8CGE9. baseline and differential.
GenevisibleiQ8CGE9. MM.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR003109. GoLoco_motif.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR003116. RBD_dom.
IPR016137. RGS.
IPR024066. RGS_subdom1.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF02188. GoLoco. 1 hit.
PF00595. PDZ. 1 hit.
PF02196. RBD. 2 hits.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00390. GoLoco. 1 hit.
SM00228. PDZ. 1 hit.
SM00462. PTB. 1 hit.
SM00455. RBD. 2 hits.
SM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 2 hits.
PROSITEiPS50877. GOLOCO. 1 hit.
PS50106. PDZ. 1 hit.
PS01179. PID. 1 hit.
PS50898. RBD. 2 hits.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171; SER-194; SER-661; SER-671; SER-850; SER-879 AND SER-943, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiRGS12_MOUSE
AccessioniPrimary (citable) accession number: Q8CGE9
Secondary accession number(s): E9PXX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.