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Protein

RNA-binding protein 28

Gene

Rbm28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolar component of the spliceosomal ribonucleoprotein complexes.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 28
Alternative name(s):
RNA-binding motif protein 28
Gene namesi
Name:Rbm28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2655711. Rbm28.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000817862 – 750RNA-binding protein 28Add BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei122PhosphoserineBy similarity1
Modified residuei231PhosphoserineCombined sources1
Modified residuei239PhosphoserineCombined sources1
Modified residuei387PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CGC6.
MaxQBiQ8CGC6.
PaxDbiQ8CGC6.
PeptideAtlasiQ8CGC6.
PRIDEiQ8CGC6.

PTM databases

iPTMnetiQ8CGC6.
PhosphoSitePlusiQ8CGC6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029701.
CleanExiMM_RBM28.
ExpressionAtlasiQ8CGC6. baseline and differential.
GenevisibleiQ8CGC6. MM.

Interactioni

Subunit structurei

Interacts with U1, U2, U4, U5, and U6 spliceosomal small nuclear RNAs (snRNAs).By similarity

Protein-protein interaction databases

BioGridi212775. 2 interactors.
DIPiDIP-58949N.
STRINGi10090.ENSMUSP00000007993.

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi327 – 331Combined sources5
Helixi338 – 346Combined sources9
Beta strandi351 – 355Combined sources5
Beta strandi360 – 362Combined sources3
Beta strandi367 – 375Combined sources9
Helixi376 – 386Combined sources11
Turni388 – 392Combined sources5
Beta strandi395 – 399Combined sources5
Beta strandi401 – 405Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X4HNMR-A317-414[»]
ProteinModelPortaliQ8CGC6.
SMRiQ8CGC6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CGC6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 80RRM 1PROSITE-ProRule annotationAdd BLAST77
Domaini114 – 191RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini325 – 409RRM 3PROSITE-ProRule annotationAdd BLAST85
Domaini477 – 582RRM 4PROSITE-ProRule annotationAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi225 – 306Asp/Glu-rich (acidic)Add BLAST82

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0127. Eukaryota.
ENOG410XSHP. LUCA.
GeneTreeiENSGT00550000074976.
HOVERGENiHBG073213.
InParanoidiQ8CGC6.
KOiK14573.
OMAiYEHFTQF.
OrthoDBiEOG091G0JKS.
TreeFamiTF312798.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CGC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLTLFVGR LPPSARSDQL EELFSQVGPV KQCFVVTEKG SKACRGFGYV
60 70 80 90 100
TFSMLEDVQR ALKEITTFEG CKIDVTVAKK KLRNKSKETR KNENAESPKK
110 120 130 140 150
EPKHKKAKVA DKKARLIIRN LSFKCSEDDL KAVFTHYGTV LEVNIPKKPD
160 170 180 190 200
GKMRGFAFVQ FKNLLEAGKA LKGANMKEIK GRTVAVDWAV AKDKYKDAQH
210 220 230 240 250
ASAPGVKKSS DRKPKESGKK NCRVEEQVED SDDEEDDDSH DDEEERESTI
260 270 280 290 300
ASPVSVHKRA VKRAAPEESI EEDDSYEDSD LEEGGSSYDE GTVDSESSAE
310 320 330 340 350
DQEDEDVPVS EKKKRKLPSD VTEGKTVFIR NLSFDSEEEA LGEVLQQFGD
360 370 380 390 400
LKYVRVVLHP DTEHSKGCAF AQFMTQEAAQ KCLAAASLEA EGGGLKLDGR
410 420 430 440 450
QLKVDLAVTR DEAAKLQTKK VKKPTGTRNL YLAREGLIRA GTKAAEGVSA
460 470 480 490 500
ADMAKRERFE LLKHQKLKNQ NIFVSQTRLC LHNLPKAVDD KQLRKLLLEA
510 520 530 540 550
TRGEKGVRIK ECRVMRDLKG VHGKMKGQSL GYAFAEFQKH EHALRALRHF
560 570 580 590 600
NNNPEIFGSQ KRPIVEFSLE DRRKLKVKEL RIQRSLQKME SKPVTSKPQK
610 620 630 640 650
EQKEVGKDKQ QKVVQSATQD QSKAPGEQKG KAGSTSWSGF QTKAEVEQVE
660 670 680 690 700
LPDGKKRRKV LALPSHRGPK IRLRDKGKVK SLPSRKPKPQ MGQRKQKKQQ
710 720 730 740 750
LASSVQAPKR KAKENKAEAR FNQLVEQYKQ KLLGPSKGAP LMKRSKWFDS
Length:750
Mass (Da):84,214
Last modified:July 27, 2011 - v4
Checksum:iC767D7C6333E9944
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239S → I in BAB31653 (PubMed:16141072).Curated1
Sequence conflicti242 – 243DE → GR in BAB31653 (PubMed:16141072).Curated2
Sequence conflicti388L → P in AAH40811 (PubMed:15489334).Curated1
Sequence conflicti484L → F in AAH40811 (PubMed:15489334).Curated1
Sequence conflicti613V → A in BAE33848 (PubMed:16141072).Curated1
Sequence conflicti613V → A in AAH18373 (PubMed:15489334).Curated1
Sequence conflicti613V → A in AAH40811 (PubMed:15489334).Curated1
Sequence conflicti638S → T in BAE33848 (PubMed:16141072).Curated1
Sequence conflicti638S → T in AAH18373 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012408 mRNA. Translation: BAC25367.1.
AK013047 mRNA. Translation: BAB28620.1.
AK019296 mRNA. Translation: BAB31653.3.
AK019436 mRNA. Translation: BAC25592.1.
AK156773 mRNA. Translation: BAE33848.1.
AC153909 Genomic DNA. No translation available.
BC018373 mRNA. Translation: AAH18373.1.
BC040811 mRNA. Translation: AAH40811.1.
CCDSiCCDS51733.1.
RefSeqiNP_598686.2. NM_133925.2.
UniGeneiMm.40802.

Genome annotation databases

EnsembliENSMUST00000007993; ENSMUSP00000007993; ENSMUSG00000029701.
GeneIDi68272.
KEGGimmu:68272.
UCSCiuc009bcx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012408 mRNA. Translation: BAC25367.1.
AK013047 mRNA. Translation: BAB28620.1.
AK019296 mRNA. Translation: BAB31653.3.
AK019436 mRNA. Translation: BAC25592.1.
AK156773 mRNA. Translation: BAE33848.1.
AC153909 Genomic DNA. No translation available.
BC018373 mRNA. Translation: AAH18373.1.
BC040811 mRNA. Translation: AAH40811.1.
CCDSiCCDS51733.1.
RefSeqiNP_598686.2. NM_133925.2.
UniGeneiMm.40802.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X4HNMR-A317-414[»]
ProteinModelPortaliQ8CGC6.
SMRiQ8CGC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212775. 2 interactors.
DIPiDIP-58949N.
STRINGi10090.ENSMUSP00000007993.

PTM databases

iPTMnetiQ8CGC6.
PhosphoSitePlusiQ8CGC6.

Proteomic databases

EPDiQ8CGC6.
MaxQBiQ8CGC6.
PaxDbiQ8CGC6.
PeptideAtlasiQ8CGC6.
PRIDEiQ8CGC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007993; ENSMUSP00000007993; ENSMUSG00000029701.
GeneIDi68272.
KEGGimmu:68272.
UCSCiuc009bcx.2. mouse.

Organism-specific databases

CTDi55131.
MGIiMGI:2655711. Rbm28.

Phylogenomic databases

eggNOGiKOG0127. Eukaryota.
ENOG410XSHP. LUCA.
GeneTreeiENSGT00550000074976.
HOVERGENiHBG073213.
InParanoidiQ8CGC6.
KOiK14573.
OMAiYEHFTQF.
OrthoDBiEOG091G0JKS.
TreeFamiTF312798.

Miscellaneous databases

ChiTaRSiRbm28. mouse.
EvolutionaryTraceiQ8CGC6.
PROiQ8CGC6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029701.
CleanExiMM_RBM28.
ExpressionAtlasiQ8CGC6. baseline and differential.
GenevisibleiQ8CGC6. MM.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBM28_MOUSE
AccessioniPrimary (citable) accession number: Q8CGC6
Secondary accession number(s): E9QKF8
, Q3U0K5, Q8BG25, Q8VEJ8, Q9CS22, Q9CSE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.