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Protein

RNA-binding protein 28

Gene

Rbm28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolar component of the spliceosomal ribonucleoprotein complexes.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 28
Alternative name(s):
RNA-binding motif protein 28
Gene namesi
Name:Rbm28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2655711. Rbm28.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 750749RNA-binding protein 28PRO_0000081786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei122 – 1221PhosphoserineBy similarity
Modified residuei231 – 2311PhosphoserineCombined sources
Modified residuei239 – 2391PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CGC6.
MaxQBiQ8CGC6.
PaxDbiQ8CGC6.
PRIDEiQ8CGC6.

PTM databases

iPTMnetiQ8CGC6.
PhosphoSiteiQ8CGC6.

Expressioni

Gene expression databases

BgeeiQ8CGC6.
CleanExiMM_RBM28.
ExpressionAtlasiQ8CGC6. baseline and differential.
GenevisibleiQ8CGC6. MM.

Interactioni

Subunit structurei

Interacts with U1, U2, U4, U5, and U6 spliceosomal small nuclear RNAs (snRNAs).By similarity

Protein-protein interaction databases

BioGridi212775. 2 interactions.
DIPiDIP-58949N.
STRINGi10090.ENSMUSP00000007993.

Structurei

Secondary structure

1
750
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi327 – 3315Combined sources
Helixi338 – 3469Combined sources
Beta strandi351 – 3555Combined sources
Beta strandi360 – 3623Combined sources
Beta strandi367 – 3759Combined sources
Helixi376 – 38611Combined sources
Turni388 – 3925Combined sources
Beta strandi395 – 3995Combined sources
Beta strandi401 – 4055Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X4HNMR-A317-414[»]
ProteinModelPortaliQ8CGC6.
SMRiQ8CGC6. Positions 4-219, 317-414.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CGC6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8077RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini114 – 19178RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini325 – 40985RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini477 – 582106RRM 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi225 – 30682Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0127. Eukaryota.
ENOG410XSHP. LUCA.
GeneTreeiENSGT00550000074976.
HOVERGENiHBG073213.
InParanoidiQ8CGC6.
KOiK14573.
OMAiPVKHATV.
OrthoDBiEOG70ZZMT.
TreeFamiTF312798.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CGC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLTLFVGR LPPSARSDQL EELFSQVGPV KQCFVVTEKG SKACRGFGYV
60 70 80 90 100
TFSMLEDVQR ALKEITTFEG CKIDVTVAKK KLRNKSKETR KNENAESPKK
110 120 130 140 150
EPKHKKAKVA DKKARLIIRN LSFKCSEDDL KAVFTHYGTV LEVNIPKKPD
160 170 180 190 200
GKMRGFAFVQ FKNLLEAGKA LKGANMKEIK GRTVAVDWAV AKDKYKDAQH
210 220 230 240 250
ASAPGVKKSS DRKPKESGKK NCRVEEQVED SDDEEDDDSH DDEEERESTI
260 270 280 290 300
ASPVSVHKRA VKRAAPEESI EEDDSYEDSD LEEGGSSYDE GTVDSESSAE
310 320 330 340 350
DQEDEDVPVS EKKKRKLPSD VTEGKTVFIR NLSFDSEEEA LGEVLQQFGD
360 370 380 390 400
LKYVRVVLHP DTEHSKGCAF AQFMTQEAAQ KCLAAASLEA EGGGLKLDGR
410 420 430 440 450
QLKVDLAVTR DEAAKLQTKK VKKPTGTRNL YLAREGLIRA GTKAAEGVSA
460 470 480 490 500
ADMAKRERFE LLKHQKLKNQ NIFVSQTRLC LHNLPKAVDD KQLRKLLLEA
510 520 530 540 550
TRGEKGVRIK ECRVMRDLKG VHGKMKGQSL GYAFAEFQKH EHALRALRHF
560 570 580 590 600
NNNPEIFGSQ KRPIVEFSLE DRRKLKVKEL RIQRSLQKME SKPVTSKPQK
610 620 630 640 650
EQKEVGKDKQ QKVVQSATQD QSKAPGEQKG KAGSTSWSGF QTKAEVEQVE
660 670 680 690 700
LPDGKKRRKV LALPSHRGPK IRLRDKGKVK SLPSRKPKPQ MGQRKQKKQQ
710 720 730 740 750
LASSVQAPKR KAKENKAEAR FNQLVEQYKQ KLLGPSKGAP LMKRSKWFDS
Length:750
Mass (Da):84,214
Last modified:July 27, 2011 - v4
Checksum:iC767D7C6333E9944
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti239 – 2391S → I in BAB31653 (PubMed:16141072).Curated
Sequence conflicti242 – 2432DE → GR in BAB31653 (PubMed:16141072).Curated
Sequence conflicti388 – 3881L → P in AAH40811 (PubMed:15489334).Curated
Sequence conflicti484 – 4841L → F in AAH40811 (PubMed:15489334).Curated
Sequence conflicti613 – 6131V → A in BAE33848 (PubMed:16141072).Curated
Sequence conflicti613 – 6131V → A in AAH18373 (PubMed:15489334).Curated
Sequence conflicti613 – 6131V → A in AAH40811 (PubMed:15489334).Curated
Sequence conflicti638 – 6381S → T in BAE33848 (PubMed:16141072).Curated
Sequence conflicti638 – 6381S → T in AAH18373 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012408 mRNA. Translation: BAC25367.1.
AK013047 mRNA. Translation: BAB28620.1.
AK019296 mRNA. Translation: BAB31653.3.
AK019436 mRNA. Translation: BAC25592.1.
AK156773 mRNA. Translation: BAE33848.1.
AC153909 Genomic DNA. No translation available.
BC018373 mRNA. Translation: AAH18373.1.
BC040811 mRNA. Translation: AAH40811.1.
CCDSiCCDS51733.1.
RefSeqiNP_598686.2. NM_133925.2.
UniGeneiMm.40802.

Genome annotation databases

EnsembliENSMUST00000007993; ENSMUSP00000007993; ENSMUSG00000029701.
GeneIDi68272.
KEGGimmu:68272.
UCSCiuc009bcx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012408 mRNA. Translation: BAC25367.1.
AK013047 mRNA. Translation: BAB28620.1.
AK019296 mRNA. Translation: BAB31653.3.
AK019436 mRNA. Translation: BAC25592.1.
AK156773 mRNA. Translation: BAE33848.1.
AC153909 Genomic DNA. No translation available.
BC018373 mRNA. Translation: AAH18373.1.
BC040811 mRNA. Translation: AAH40811.1.
CCDSiCCDS51733.1.
RefSeqiNP_598686.2. NM_133925.2.
UniGeneiMm.40802.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X4HNMR-A317-414[»]
ProteinModelPortaliQ8CGC6.
SMRiQ8CGC6. Positions 4-219, 317-414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212775. 2 interactions.
DIPiDIP-58949N.
STRINGi10090.ENSMUSP00000007993.

PTM databases

iPTMnetiQ8CGC6.
PhosphoSiteiQ8CGC6.

Proteomic databases

EPDiQ8CGC6.
MaxQBiQ8CGC6.
PaxDbiQ8CGC6.
PRIDEiQ8CGC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007993; ENSMUSP00000007993; ENSMUSG00000029701.
GeneIDi68272.
KEGGimmu:68272.
UCSCiuc009bcx.2. mouse.

Organism-specific databases

CTDi55131.
MGIiMGI:2655711. Rbm28.

Phylogenomic databases

eggNOGiKOG0127. Eukaryota.
ENOG410XSHP. LUCA.
GeneTreeiENSGT00550000074976.
HOVERGENiHBG073213.
InParanoidiQ8CGC6.
KOiK14573.
OMAiPVKHATV.
OrthoDBiEOG70ZZMT.
TreeFamiTF312798.

Miscellaneous databases

ChiTaRSiRbm28. mouse.
EvolutionaryTraceiQ8CGC6.
PROiQ8CGC6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CGC6.
CleanExiMM_RBM28.
ExpressionAtlasiQ8CGC6. baseline and differential.
GenevisibleiQ8CGC6. MM.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo, Embryonic head and Spleen.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II and FVB/N.
    Tissue: Mammary gland.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231 AND SER-239, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Spleen.
  5. "Solution structure of RRM domain in RNA-binding protein 28."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 317-414.

Entry informationi

Entry nameiRBM28_MOUSE
AccessioniPrimary (citable) accession number: Q8CGC6
Secondary accession number(s): E9QKF8
, Q3U0K5, Q8BG25, Q8VEJ8, Q9CS22, Q9CSE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 114 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.