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Q8CGB3

- UACA_MOUSE

UniProt

Q8CGB3 - UACA_MOUSE

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Protein

Uveal autoantigen with coiled-coil domains and ankyrin repeats

Gene

Uaca

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.2 Publications
Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury (By similarity).By similarity

GO - Biological processi

  1. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  2. intrinsic apoptotic signaling pathway in response to oxidative stress Source: MGI
  3. negative regulation of inflammatory response Source: MGI
  4. negative regulation of NF-kappaB import into nucleus Source: MGI
  5. positive regulation of apoptotic process Source: MGI
  6. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  7. positive regulation of protein import into nucleus Source: MGI
  8. response to UV Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Uveal autoantigen with coiled-coil domains and ankyrin repeats
Alternative name(s):
Nuclear membrane-binding protein
Short name:
Nucling
Gene namesi
Name:Uaca
Synonyms:Kiaa1561
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1919815. Uaca.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication
Note: Expressed diffusely in cytoplasm.

GO - Cellular componenti

  1. apoptosome Source: MGI
  2. cytoplasm Source: MGI
  3. cytoskeleton Source: UniProtKB-KW
  4. cytosol Source: MGI
  5. extracellular vesicular exosome Source: Ensembl
  6. membrane Source: MGI
  7. mitochondrion Source: Ensembl
  8. nuclear envelope Source: MGI
  9. nucleus Source: MGI
  10. perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show a high incidence of inflammatory lesions in preputial glands. Cells around the lesions showed resistance to apoptosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14111411Uveal autoantigen with coiled-coil domains and ankyrin repeatsPRO_0000231651Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei280 – 2801Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8CGB3.
PaxDbiQ8CGB3.
PRIDEiQ8CGB3.

PTM databases

PhosphoSiteiQ8CGB3.

Expressioni

Tissue specificityi

Highly expressed in heart, liver, kidney and testis. Weakly expressed in lung and skeletal muscle. Not expressed in brain and spleen.1 Publication

Developmental stagei

First detected at the E9.5 stage in heart at the edge of both sides of the common ventricular chamber and is then progressively increased and restricted to the myocardial wall of left common ventricular chamber of heart.1 Publication

Inductioni

Up-regulated during cardiomyogenic differentiation. By apoptotic stress in a dose-dependent manner.1 Publication

Gene expression databases

BgeeiQ8CGB3.
GenevestigatoriQ8CGB3.

Interactioni

Subunit structurei

Component of the apoptosome complex, composed of APAF1, pro-caspase-9 and UACA. In the complex, it probably interacts directly with APAF1. Interacts with LGALS3, ARF6 and ACTB.2 Publications

Protein-protein interaction databases

BioGridi215442. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8CGB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati69 – 9830ANK 1Add
BLAST
Repeati102 – 13130ANK 2Add
BLAST
Repeati135 – 16430ANK 3Add
BLAST
Repeati168 – 19730ANK 4Add
BLAST
Repeati201 – 23030ANK 5Add
BLAST
Repeati234 – 26330ANK 6Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili299 – 37981Sequence AnalysisAdd
BLAST
Coiled coili442 – 624183Sequence AnalysisAdd
BLAST
Coiled coili652 – 1380729Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00530000063369.
HOGENOMiHOG000147885.
HOVERGENiHBG066395.
InParanoidiQ8CGB3.
OrthoDBiEOG7X9G6C.
PhylomeDBiQ8CGB3.
TreeFamiTF331274.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8CGB3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSLKSRLWK QDAPGPTSPS SPTAVASTQS AEWNKYDDRL MKAAERGDVE
60 70 80 90 100
KVSSILAKKG VHPGKLDVEG RSAFHVVASK GNLECLNAIL THGIDVATRD
110 120 130 140 150
SAGRNALHLA AKYGHALCLQ KLLQYNCPTE HVDLQGRTAL HDAVMADCPS
160 170 180 190 200
SIQLLCDHGA SVNAKDIDGR TPLVLATQMC RPTICQLLID RGADVNSRDK
210 220 230 240 250
QNRTALMLGC EYGCRDAVEV LVKNGADLTL LDALGHDSSY YARIGDNLDI
260 270 280 290 300
LNLLKTASEN TNKGRELWRK GPPLQQRNLS HTQDEGSVKS TQREQREPHS
310 320 330 340 350
FQDLEIENED LREKLRKIQQ EQRILLDKVN GLQLQLNEEV MVADDLESER
360 370 380 390 400
EKPKSLLAAK EKQHEESLRT IEALKNRFKY FESDHPGPGS YPSNRKEDML
410 420 430 440 450
HKQGQMYTTE PQCASPGIPP HMHSRSMLRP LELSLPSQTS YSENEILKKE
460 470 480 490 500
LETLRTYYDS AKQDRLKFQN ELAHKVAECK ALALECERVK EDSDEQIKQL
510 520 530 540 550
EDALKDVQKR MYESEGKVKQ MQTHFLALKE HLTNEAATGS HRIIEELREQ
560 570 580 590 600
LKDLKGKYEG ASAEVGKLRS QIKQSEMLVG EFKRDEGRLV EENKRLQKEC
610 620 630 640 650
GTCEVELERR GRRVVELEGQ LKELGAKLAL SVPTEKFESM KSSLSNDINE
660 670 680 690 700
KVKRLAEVGR DYESAQGEIR QLKRDLESVR AQHIRPEEHE QLRSRLEQKS
710 720 730 740 750
GELGKKVSEL TLKNQTLQKD VEKLHADNKL LNQQVHSLTV EMKTRYVPLR
760 770 780 790 800
VSEEMKRSHD VNVEDLNKKL SEATQRYAEK KQEAERLLAE NDKLTKNVSR
810 820 830 840 850
LEAVFVAPEK HEKELMGLKS NIAELKKQLS ELNKKCGEGQ EKIRALMSEN
860 870 880 890 900
SSLKKTLSSQ YVPAKTHEEV KASLNSTVEK TNRALLEAKK RFDDTSQEVS
910 920 930 940 950
KLRDENEVLR RNLENVQNQM KADYVSLEEH SRRMSTVSQS LKEAQEANAA
960 970 980 990 1000
ILADHRQGQE EIVSLHAEIK AQKKELDTIQ ECIKLKYAPL ARLEECERKF
1010 1020 1030 1040 1050
KATEKGLKEQ LSEQTHKCRQ RDEEVKKGKQ ENERLRADLA ALQKELQDRN
1060 1070 1080 1090 1100
ALAEEAREAE RALSGKADEL SKQLKDLSQK YSDVKSEREK LVEEKAKQAS
1110 1120 1130 1140 1150
EILAAQNLLQ KQPVPLEQVE ALKKSLNGTI EQLKEELRSK QRCLEREQQT
1160 1170 1180 1190 1200
VSQLQQLLEN QKNSSVTLAE HLKLKEALEK EVGIMKASLR EKEEESQKKT
1210 1220 1230 1240 1250
KEVSKLQTEV QTTKQALKNL ETREVVDMSK YKATKNDLET QISNLNDKLA
1260 1270 1280 1290 1300
SLNRKYDQAC EEKVSAKDEK ELLHLSIEQE IRDQKERCDK SLTTIMELQQ
1310 1320 1330 1340 1350
RIQESAKQIE AKDNKITELL NDVERLKQAL NGLSQLTYSS GSPTKRQSQL
1360 1370 1380 1390 1400
VDTLQQRVRD LQQQLADADR QHQEVIAIYR THLLSAAQGH MDEDVQAALL
1410
QIIQMRQGLV C
Length:1,411
Mass (Da):160,813
Last modified:April 4, 2006 - v2
Checksum:i0809D2DE9732F879
GO
Isoform 2 (identifier: Q8CGB3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-398: Missing.

Note: No experimental confirmation available.

Show »
Length:1,013
Mass (Da):116,517
Checksum:i13DDD2313B8FED0F
GO
Isoform 3 (identifier: Q8CGB3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     264-264: G → GGG

Note: No experimental confirmation available.

Show »
Length:1,413
Mass (Da):160,927
Checksum:i89FA7474FDE59D5C
GO

Sequence cautioni

The sequence BAC78213.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAD32481.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441V → A in AAH42415. (PubMed:15489334)Curated
Sequence conflicti353 – 3531P → L in AAH42415. (PubMed:15489334)Curated
Sequence conflicti757 – 7571R → K in AAH42415. (PubMed:15489334)Curated
Sequence conflicti778 – 7781A → T in AAH42415. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 398398Missing in isoform 2. 1 PublicationVSP_017874Add
BLAST
Alternative sequencei264 – 2641G → GGG in isoform 3. 1 PublicationVSP_017875

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030647 mRNA. Translation: BAC78213.1. Different initiation.
AK173203 mRNA. Translation: BAD32481.1. Different initiation.
BC042415 mRNA. Translation: AAH42415.1.
AK087466 mRNA. Translation: BAC39886.1.
CCDSiCCDS23259.1. [Q8CGB3-3]
RefSeqiNP_082559.1. NM_028283.2.
XP_006511543.1. XM_006511480.1.
UniGeneiMm.68819.

Genome annotation databases

EnsembliENSMUST00000050183; ENSMUSP00000062047; ENSMUSG00000034485.
GeneIDi72565.
KEGGimmu:72565.
UCSCiuc009pzi.1. mouse. [Q8CGB3-3]
uc009pzk.1. mouse. [Q8CGB3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030647 mRNA. Translation: BAC78213.1 . Different initiation.
AK173203 mRNA. Translation: BAD32481.1 . Different initiation.
BC042415 mRNA. Translation: AAH42415.1 .
AK087466 mRNA. Translation: BAC39886.1 .
CCDSi CCDS23259.1. [Q8CGB3-3 ]
RefSeqi NP_082559.1. NM_028283.2.
XP_006511543.1. XM_006511480.1.
UniGenei Mm.68819.

3D structure databases

ProteinModelPortali Q8CGB3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 215442. 3 interactions.

PTM databases

PhosphoSitei Q8CGB3.

Proteomic databases

MaxQBi Q8CGB3.
PaxDbi Q8CGB3.
PRIDEi Q8CGB3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050183 ; ENSMUSP00000062047 ; ENSMUSG00000034485 .
GeneIDi 72565.
KEGGi mmu:72565.
UCSCi uc009pzi.1. mouse. [Q8CGB3-3 ]
uc009pzk.1. mouse. [Q8CGB3-1 ]

Organism-specific databases

CTDi 55075.
MGIi MGI:1919815. Uaca.
Rougei Search...

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00530000063369.
HOGENOMi HOG000147885.
HOVERGENi HBG066395.
InParanoidi Q8CGB3.
OrthoDBi EOG7X9G6C.
PhylomeDBi Q8CGB3.
TreeFami TF331274.

Miscellaneous databases

ChiTaRSi UACA. mouse.
NextBioi 336503.
PROi Q8CGB3.
SOURCEi Search...

Gene expression databases

Bgeei Q8CGB3.
Genevestigatori Q8CGB3.

Family and domain databases

Gene3Di 1.25.40.20. 2 hits.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000727. T_SNARE_dom.
[Graphical view ]
Pfami PF12796. Ank_2. 2 hits.
[Graphical view ]
PRINTSi PR01415. ANKYRIN.
SMARTi SM00248. ANK. 6 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50192. T_SNARE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel, embryonal carcinoma cell-associated molecule, nucling, that is up-regulated during cardiac muscle differentiation."
    Sakai T., Liu L., Shishido Y., Fukui K.
    J. Biochem. 133:429-436(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: 129.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryonic intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary gland and Salivary gland.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-306.
    Strain: C57BL/6J.
    Tissue: Eye.
  5. "Nucling recruits Apaf-1/pro-caspase-9 complex for the induction of stress-induced apoptosis."
    Sakai T., Liu L., Teng X., Mukai-Sakai R., Shimada H., Kaji R., Mitani T., Matsumoto M., Toida K., Ishimura K., Shishido Y., Mak T.W., Fukui K.
    J. Biol. Chem. 279:41131-41140(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH APAF1.
  6. "Nucling mediates apoptosis by inhibiting expression of galectin-3 through interference with nuclear factor kappaB signalling."
    Liu L., Sakai T., Sano N., Fukui K.
    Biochem. J. 380:31-41(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LGALS3, DISRUPTION PHENOTYPE.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUACA_MOUSE
AccessioniPrimary (citable) accession number: Q8CGB3
Secondary accession number(s): Q69ZG3, Q7TN77, Q8BJC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: October 29, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3