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Q8CGB3

- UACA_MOUSE

UniProt

Q8CGB3 - UACA_MOUSE

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Protein
Uveal autoantigen with coiled-coil domains and ankyrin repeats
Gene
Uaca, Kiaa1561
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.2 Publications
Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury By similarity.2 Publications

GO - Molecular functioni

  1. protein binding Source: MGI

GO - Biological processi

  1. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  2. intrinsic apoptotic signaling pathway in response to oxidative stress Source: MGI
  3. negative regulation of NF-kappaB import into nucleus Source: MGI
  4. negative regulation of inflammatory response Source: MGI
  5. positive regulation of apoptotic process Source: MGI
  6. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  7. positive regulation of protein import into nucleus Source: MGI
  8. response to UV Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Uveal autoantigen with coiled-coil domains and ankyrin repeats
Alternative name(s):
Nuclear membrane-binding protein
Short name:
Nucling
Gene namesi
Name:Uaca
Synonyms:Kiaa1561
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1919815. Uaca.

Subcellular locationi

Nucleus. Cytoplasm. Cytoplasmcytoskeleton
Note: Expressed diffusely in cytoplasm.1 Publication

GO - Cellular componenti

  1. apoptosome Source: MGI
  2. cytoplasm Source: MGI
  3. cytoskeleton Source: UniProtKB-SubCell
  4. cytosol Source: MGI
  5. membrane Source: MGI
  6. mitochondrion Source: Ensembl
  7. nuclear envelope Source: MGI
  8. nucleus Source: MGI
  9. perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show a high incidence of inflammatory lesions in preputial glands. Cells around the lesions showed resistance to apoptosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14111411Uveal autoantigen with coiled-coil domains and ankyrin repeats
PRO_0000231651Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei280 – 2801Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8CGB3.
PaxDbiQ8CGB3.
PRIDEiQ8CGB3.

PTM databases

PhosphoSiteiQ8CGB3.

Expressioni

Tissue specificityi

Highly expressed in heart, liver, kidney and testis. Weakly expressed in lung and skeletal muscle. Not expressed in brain and spleen.1 Publication

Developmental stagei

First detected at the E9.5 stage in heart at the edge of both sides of the common ventricular chamber and is then progressively increased and restricted to the myocardial wall of left common ventricular chamber of heart.1 Publication

Inductioni

Up-regulated during cardiomyogenic differentiation. By apoptotic stress in a dose-dependent manner.1 Publication

Gene expression databases

BgeeiQ8CGB3.
GenevestigatoriQ8CGB3.

Interactioni

Subunit structurei

Component of the apoptosome complex, composed of APAF1, pro-caspase-9 and UACA. In the complex, it probably interacts directly with APAF1. Interacts with LGALS3, ARF6 and ACTB.2 Publications

Protein-protein interaction databases

BioGridi215442. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8CGB3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati69 – 9830ANK 1
Add
BLAST
Repeati102 – 13130ANK 2
Add
BLAST
Repeati135 – 16430ANK 3
Add
BLAST
Repeati168 – 19730ANK 4
Add
BLAST
Repeati201 – 23030ANK 5
Add
BLAST
Repeati234 – 26330ANK 6
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili299 – 37981 Reviewed prediction
Add
BLAST
Coiled coili442 – 624183 Reviewed prediction
Add
BLAST
Coiled coili652 – 1380729 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 6 ANK repeats.

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00530000063369.
HOGENOMiHOG000147885.
HOVERGENiHBG066395.
InParanoidiQ8CGB3.
OrthoDBiEOG7X9G6C.
PhylomeDBiQ8CGB3.
TreeFamiTF331274.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8CGB3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKSLKSRLWK QDAPGPTSPS SPTAVASTQS AEWNKYDDRL MKAAERGDVE     50
KVSSILAKKG VHPGKLDVEG RSAFHVVASK GNLECLNAIL THGIDVATRD 100
SAGRNALHLA AKYGHALCLQ KLLQYNCPTE HVDLQGRTAL HDAVMADCPS 150
SIQLLCDHGA SVNAKDIDGR TPLVLATQMC RPTICQLLID RGADVNSRDK 200
QNRTALMLGC EYGCRDAVEV LVKNGADLTL LDALGHDSSY YARIGDNLDI 250
LNLLKTASEN TNKGRELWRK GPPLQQRNLS HTQDEGSVKS TQREQREPHS 300
FQDLEIENED LREKLRKIQQ EQRILLDKVN GLQLQLNEEV MVADDLESER 350
EKPKSLLAAK EKQHEESLRT IEALKNRFKY FESDHPGPGS YPSNRKEDML 400
HKQGQMYTTE PQCASPGIPP HMHSRSMLRP LELSLPSQTS YSENEILKKE 450
LETLRTYYDS AKQDRLKFQN ELAHKVAECK ALALECERVK EDSDEQIKQL 500
EDALKDVQKR MYESEGKVKQ MQTHFLALKE HLTNEAATGS HRIIEELREQ 550
LKDLKGKYEG ASAEVGKLRS QIKQSEMLVG EFKRDEGRLV EENKRLQKEC 600
GTCEVELERR GRRVVELEGQ LKELGAKLAL SVPTEKFESM KSSLSNDINE 650
KVKRLAEVGR DYESAQGEIR QLKRDLESVR AQHIRPEEHE QLRSRLEQKS 700
GELGKKVSEL TLKNQTLQKD VEKLHADNKL LNQQVHSLTV EMKTRYVPLR 750
VSEEMKRSHD VNVEDLNKKL SEATQRYAEK KQEAERLLAE NDKLTKNVSR 800
LEAVFVAPEK HEKELMGLKS NIAELKKQLS ELNKKCGEGQ EKIRALMSEN 850
SSLKKTLSSQ YVPAKTHEEV KASLNSTVEK TNRALLEAKK RFDDTSQEVS 900
KLRDENEVLR RNLENVQNQM KADYVSLEEH SRRMSTVSQS LKEAQEANAA 950
ILADHRQGQE EIVSLHAEIK AQKKELDTIQ ECIKLKYAPL ARLEECERKF 1000
KATEKGLKEQ LSEQTHKCRQ RDEEVKKGKQ ENERLRADLA ALQKELQDRN 1050
ALAEEAREAE RALSGKADEL SKQLKDLSQK YSDVKSEREK LVEEKAKQAS 1100
EILAAQNLLQ KQPVPLEQVE ALKKSLNGTI EQLKEELRSK QRCLEREQQT 1150
VSQLQQLLEN QKNSSVTLAE HLKLKEALEK EVGIMKASLR EKEEESQKKT 1200
KEVSKLQTEV QTTKQALKNL ETREVVDMSK YKATKNDLET QISNLNDKLA 1250
SLNRKYDQAC EEKVSAKDEK ELLHLSIEQE IRDQKERCDK SLTTIMELQQ 1300
RIQESAKQIE AKDNKITELL NDVERLKQAL NGLSQLTYSS GSPTKRQSQL 1350
VDTLQQRVRD LQQQLADADR QHQEVIAIYR THLLSAAQGH MDEDVQAALL 1400
QIIQMRQGLV C 1411
Length:1,411
Mass (Da):160,813
Last modified:April 4, 2006 - v2
Checksum:i0809D2DE9732F879
GO
Isoform 2 (identifier: Q8CGB3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-398: Missing.

Note: No experimental confirmation available.

Show »
Length:1,013
Mass (Da):116,517
Checksum:i13DDD2313B8FED0F
GO
Isoform 3 (identifier: Q8CGB3-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     264-264: G → GGG

Note: No experimental confirmation available.

Show »
Length:1,413
Mass (Da):160,927
Checksum:i89FA7474FDE59D5C
GO

Sequence cautioni

The sequence BAC78213.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAD32481.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 398398Missing in isoform 2.
VSP_017874Add
BLAST
Alternative sequencei264 – 2641G → GGG in isoform 3.
VSP_017875

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441V → A in AAH42415. 1 Publication
Sequence conflicti353 – 3531P → L in AAH42415. 1 Publication
Sequence conflicti757 – 7571R → K in AAH42415. 1 Publication
Sequence conflicti778 – 7781A → T in AAH42415. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030647 mRNA. Translation: BAC78213.1. Different initiation.
AK173203 mRNA. Translation: BAD32481.1. Different initiation.
BC042415 mRNA. Translation: AAH42415.1.
AK087466 mRNA. Translation: BAC39886.1.
CCDSiCCDS23259.1. [Q8CGB3-3]
RefSeqiNP_082559.1. NM_028283.2.
XP_006511543.1. XM_006511480.1.
UniGeneiMm.68819.

Genome annotation databases

EnsembliENSMUST00000050183; ENSMUSP00000062047; ENSMUSG00000034485.
GeneIDi72565.
KEGGimmu:72565.
UCSCiuc009pzi.1. mouse. [Q8CGB3-3]
uc009pzk.1. mouse. [Q8CGB3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030647 mRNA. Translation: BAC78213.1 . Different initiation.
AK173203 mRNA. Translation: BAD32481.1 . Different initiation.
BC042415 mRNA. Translation: AAH42415.1 .
AK087466 mRNA. Translation: BAC39886.1 .
CCDSi CCDS23259.1. [Q8CGB3-3 ]
RefSeqi NP_082559.1. NM_028283.2.
XP_006511543.1. XM_006511480.1.
UniGenei Mm.68819.

3D structure databases

ProteinModelPortali Q8CGB3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 215442. 3 interactions.

PTM databases

PhosphoSitei Q8CGB3.

Proteomic databases

MaxQBi Q8CGB3.
PaxDbi Q8CGB3.
PRIDEi Q8CGB3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050183 ; ENSMUSP00000062047 ; ENSMUSG00000034485 .
GeneIDi 72565.
KEGGi mmu:72565.
UCSCi uc009pzi.1. mouse. [Q8CGB3-3 ]
uc009pzk.1. mouse. [Q8CGB3-1 ]

Organism-specific databases

CTDi 55075.
MGIi MGI:1919815. Uaca.
Rougei Search...

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00530000063369.
HOGENOMi HOG000147885.
HOVERGENi HBG066395.
InParanoidi Q8CGB3.
OrthoDBi EOG7X9G6C.
PhylomeDBi Q8CGB3.
TreeFami TF331274.

Miscellaneous databases

ChiTaRSi UACA. mouse.
NextBioi 336503.
PROi Q8CGB3.
SOURCEi Search...

Gene expression databases

Bgeei Q8CGB3.
Genevestigatori Q8CGB3.

Family and domain databases

Gene3Di 1.25.40.20. 2 hits.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000727. T_SNARE_dom.
[Graphical view ]
Pfami PF12796. Ank_2. 2 hits.
[Graphical view ]
PRINTSi PR01415. ANKYRIN.
SMARTi SM00248. ANK. 6 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50192. T_SNARE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel, embryonal carcinoma cell-associated molecule, nucling, that is up-regulated during cardiac muscle differentiation."
    Sakai T., Liu L., Shishido Y., Fukui K.
    J. Biochem. 133:429-436(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: 129.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryonic intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary gland and Salivary gland.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-306.
    Strain: C57BL/6J.
    Tissue: Eye.
  5. "Nucling recruits Apaf-1/pro-caspase-9 complex for the induction of stress-induced apoptosis."
    Sakai T., Liu L., Teng X., Mukai-Sakai R., Shimada H., Kaji R., Mitani T., Matsumoto M., Toida K., Ishimura K., Shishido Y., Mak T.W., Fukui K.
    J. Biol. Chem. 279:41131-41140(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH APAF1.
  6. "Nucling mediates apoptosis by inhibiting expression of galectin-3 through interference with nuclear factor kappaB signalling."
    Liu L., Sakai T., Sano N., Fukui K.
    Biochem. J. 380:31-41(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LGALS3, DISRUPTION PHENOTYPE.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUACA_MOUSE
AccessioniPrimary (citable) accession number: Q8CGB3
Secondary accession number(s): Q69ZG3, Q7TN77, Q8BJC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: April 4, 2006
Last modified: July 9, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi