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Q8CGB3

- UACA_MOUSE

UniProt

Q8CGB3 - UACA_MOUSE

Protein

Uveal autoantigen with coiled-coil domains and ankyrin repeats

Gene

Uaca

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (04 Apr 2006)
      Previous versions | rss
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    Functioni

    Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.2 Publications
    Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
    2. intrinsic apoptotic signaling pathway in response to oxidative stress Source: MGI
    3. negative regulation of inflammatory response Source: MGI
    4. negative regulation of NF-kappaB import into nucleus Source: MGI
    5. positive regulation of apoptotic process Source: MGI
    6. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
    7. positive regulation of protein import into nucleus Source: MGI
    8. response to UV Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uveal autoantigen with coiled-coil domains and ankyrin repeats
    Alternative name(s):
    Nuclear membrane-binding protein
    Short name:
    Nucling
    Gene namesi
    Name:Uaca
    Synonyms:Kiaa1561
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1919815. Uaca.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: Expressed diffusely in cytoplasm.

    GO - Cellular componenti

    1. apoptosome Source: MGI
    2. cytoplasm Source: MGI
    3. cytoskeleton Source: UniProtKB-SubCell
    4. cytosol Source: MGI
    5. membrane Source: MGI
    6. mitochondrion Source: Ensembl
    7. nuclear envelope Source: MGI
    8. nucleus Source: MGI
    9. perinuclear region of cytoplasm Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice show a high incidence of inflammatory lesions in preputial glands. Cells around the lesions showed resistance to apoptosis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14111411Uveal autoantigen with coiled-coil domains and ankyrin repeatsPRO_0000231651Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei280 – 2801Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8CGB3.
    PaxDbiQ8CGB3.
    PRIDEiQ8CGB3.

    PTM databases

    PhosphoSiteiQ8CGB3.

    Expressioni

    Tissue specificityi

    Highly expressed in heart, liver, kidney and testis. Weakly expressed in lung and skeletal muscle. Not expressed in brain and spleen.1 Publication

    Developmental stagei

    First detected at the E9.5 stage in heart at the edge of both sides of the common ventricular chamber and is then progressively increased and restricted to the myocardial wall of left common ventricular chamber of heart.1 Publication

    Inductioni

    Up-regulated during cardiomyogenic differentiation. By apoptotic stress in a dose-dependent manner.1 Publication

    Gene expression databases

    BgeeiQ8CGB3.
    GenevestigatoriQ8CGB3.

    Interactioni

    Subunit structurei

    Component of the apoptosome complex, composed of APAF1, pro-caspase-9 and UACA. In the complex, it probably interacts directly with APAF1. Interacts with LGALS3, ARF6 and ACTB.2 Publications

    Protein-protein interaction databases

    BioGridi215442. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CGB3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati69 – 9830ANK 1Add
    BLAST
    Repeati102 – 13130ANK 2Add
    BLAST
    Repeati135 – 16430ANK 3Add
    BLAST
    Repeati168 – 19730ANK 4Add
    BLAST
    Repeati201 – 23030ANK 5Add
    BLAST
    Repeati234 – 26330ANK 6Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili299 – 37981Sequence AnalysisAdd
    BLAST
    Coiled coili442 – 624183Sequence AnalysisAdd
    BLAST
    Coiled coili652 – 1380729Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 6 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    GeneTreeiENSGT00530000063369.
    HOGENOMiHOG000147885.
    HOVERGENiHBG066395.
    InParanoidiQ8CGB3.
    OrthoDBiEOG7X9G6C.
    PhylomeDBiQ8CGB3.
    TreeFamiTF331274.

    Family and domain databases

    Gene3Di1.25.40.20. 2 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PfamiPF12796. Ank_2. 2 hits.
    [Graphical view]
    PRINTSiPR01415. ANKYRIN.
    SMARTiSM00248. ANK. 6 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 5 hits.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8CGB3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKSLKSRLWK QDAPGPTSPS SPTAVASTQS AEWNKYDDRL MKAAERGDVE     50
    KVSSILAKKG VHPGKLDVEG RSAFHVVASK GNLECLNAIL THGIDVATRD 100
    SAGRNALHLA AKYGHALCLQ KLLQYNCPTE HVDLQGRTAL HDAVMADCPS 150
    SIQLLCDHGA SVNAKDIDGR TPLVLATQMC RPTICQLLID RGADVNSRDK 200
    QNRTALMLGC EYGCRDAVEV LVKNGADLTL LDALGHDSSY YARIGDNLDI 250
    LNLLKTASEN TNKGRELWRK GPPLQQRNLS HTQDEGSVKS TQREQREPHS 300
    FQDLEIENED LREKLRKIQQ EQRILLDKVN GLQLQLNEEV MVADDLESER 350
    EKPKSLLAAK EKQHEESLRT IEALKNRFKY FESDHPGPGS YPSNRKEDML 400
    HKQGQMYTTE PQCASPGIPP HMHSRSMLRP LELSLPSQTS YSENEILKKE 450
    LETLRTYYDS AKQDRLKFQN ELAHKVAECK ALALECERVK EDSDEQIKQL 500
    EDALKDVQKR MYESEGKVKQ MQTHFLALKE HLTNEAATGS HRIIEELREQ 550
    LKDLKGKYEG ASAEVGKLRS QIKQSEMLVG EFKRDEGRLV EENKRLQKEC 600
    GTCEVELERR GRRVVELEGQ LKELGAKLAL SVPTEKFESM KSSLSNDINE 650
    KVKRLAEVGR DYESAQGEIR QLKRDLESVR AQHIRPEEHE QLRSRLEQKS 700
    GELGKKVSEL TLKNQTLQKD VEKLHADNKL LNQQVHSLTV EMKTRYVPLR 750
    VSEEMKRSHD VNVEDLNKKL SEATQRYAEK KQEAERLLAE NDKLTKNVSR 800
    LEAVFVAPEK HEKELMGLKS NIAELKKQLS ELNKKCGEGQ EKIRALMSEN 850
    SSLKKTLSSQ YVPAKTHEEV KASLNSTVEK TNRALLEAKK RFDDTSQEVS 900
    KLRDENEVLR RNLENVQNQM KADYVSLEEH SRRMSTVSQS LKEAQEANAA 950
    ILADHRQGQE EIVSLHAEIK AQKKELDTIQ ECIKLKYAPL ARLEECERKF 1000
    KATEKGLKEQ LSEQTHKCRQ RDEEVKKGKQ ENERLRADLA ALQKELQDRN 1050
    ALAEEAREAE RALSGKADEL SKQLKDLSQK YSDVKSEREK LVEEKAKQAS 1100
    EILAAQNLLQ KQPVPLEQVE ALKKSLNGTI EQLKEELRSK QRCLEREQQT 1150
    VSQLQQLLEN QKNSSVTLAE HLKLKEALEK EVGIMKASLR EKEEESQKKT 1200
    KEVSKLQTEV QTTKQALKNL ETREVVDMSK YKATKNDLET QISNLNDKLA 1250
    SLNRKYDQAC EEKVSAKDEK ELLHLSIEQE IRDQKERCDK SLTTIMELQQ 1300
    RIQESAKQIE AKDNKITELL NDVERLKQAL NGLSQLTYSS GSPTKRQSQL 1350
    VDTLQQRVRD LQQQLADADR QHQEVIAIYR THLLSAAQGH MDEDVQAALL 1400
    QIIQMRQGLV C 1411
    Length:1,411
    Mass (Da):160,813
    Last modified:April 4, 2006 - v2
    Checksum:i0809D2DE9732F879
    GO
    Isoform 2 (identifier: Q8CGB3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-398: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,013
    Mass (Da):116,517
    Checksum:i13DDD2313B8FED0F
    GO
    Isoform 3 (identifier: Q8CGB3-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         264-264: G → GGG

    Note: No experimental confirmation available.

    Show »
    Length:1,413
    Mass (Da):160,927
    Checksum:i89FA7474FDE59D5C
    GO

    Sequence cautioni

    The sequence BAC78213.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAD32481.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti144 – 1441V → A in AAH42415. (PubMed:15489334)Curated
    Sequence conflicti353 – 3531P → L in AAH42415. (PubMed:15489334)Curated
    Sequence conflicti757 – 7571R → K in AAH42415. (PubMed:15489334)Curated
    Sequence conflicti778 – 7781A → T in AAH42415. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 398398Missing in isoform 2. 1 PublicationVSP_017874Add
    BLAST
    Alternative sequencei264 – 2641G → GGG in isoform 3. 1 PublicationVSP_017875

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030647 mRNA. Translation: BAC78213.1. Different initiation.
    AK173203 mRNA. Translation: BAD32481.1. Different initiation.
    BC042415 mRNA. Translation: AAH42415.1.
    AK087466 mRNA. Translation: BAC39886.1.
    CCDSiCCDS23259.1. [Q8CGB3-3]
    RefSeqiNP_082559.1. NM_028283.2.
    XP_006511543.1. XM_006511480.1.
    UniGeneiMm.68819.

    Genome annotation databases

    EnsembliENSMUST00000050183; ENSMUSP00000062047; ENSMUSG00000034485.
    GeneIDi72565.
    KEGGimmu:72565.
    UCSCiuc009pzi.1. mouse. [Q8CGB3-3]
    uc009pzk.1. mouse. [Q8CGB3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB030647 mRNA. Translation: BAC78213.1 . Different initiation.
    AK173203 mRNA. Translation: BAD32481.1 . Different initiation.
    BC042415 mRNA. Translation: AAH42415.1 .
    AK087466 mRNA. Translation: BAC39886.1 .
    CCDSi CCDS23259.1. [Q8CGB3-3 ]
    RefSeqi NP_082559.1. NM_028283.2.
    XP_006511543.1. XM_006511480.1.
    UniGenei Mm.68819.

    3D structure databases

    ProteinModelPortali Q8CGB3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215442. 3 interactions.

    PTM databases

    PhosphoSitei Q8CGB3.

    Proteomic databases

    MaxQBi Q8CGB3.
    PaxDbi Q8CGB3.
    PRIDEi Q8CGB3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050183 ; ENSMUSP00000062047 ; ENSMUSG00000034485 .
    GeneIDi 72565.
    KEGGi mmu:72565.
    UCSCi uc009pzi.1. mouse. [Q8CGB3-3 ]
    uc009pzk.1. mouse. [Q8CGB3-1 ]

    Organism-specific databases

    CTDi 55075.
    MGIi MGI:1919815. Uaca.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG0666.
    GeneTreei ENSGT00530000063369.
    HOGENOMi HOG000147885.
    HOVERGENi HBG066395.
    InParanoidi Q8CGB3.
    OrthoDBi EOG7X9G6C.
    PhylomeDBi Q8CGB3.
    TreeFami TF331274.

    Miscellaneous databases

    ChiTaRSi UACA. mouse.
    NextBioi 336503.
    PROi Q8CGB3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CGB3.
    Genevestigatori Q8CGB3.

    Family and domain databases

    Gene3Di 1.25.40.20. 2 hits.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    Pfami PF12796. Ank_2. 2 hits.
    [Graphical view ]
    PRINTSi PR01415. ANKYRIN.
    SMARTi SM00248. ANK. 6 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 5 hits.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel, embryonal carcinoma cell-associated molecule, nucling, that is up-regulated during cardiac muscle differentiation."
      Sakai T., Liu L., Shishido Y., Fukui K.
      J. Biochem. 133:429-436(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: 129.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Embryonic intestine.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Strain: FVB/N.
      Tissue: Mammary gland and Salivary gland.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-306.
      Strain: C57BL/6J.
      Tissue: Eye.
    5. "Nucling recruits Apaf-1/pro-caspase-9 complex for the induction of stress-induced apoptosis."
      Sakai T., Liu L., Teng X., Mukai-Sakai R., Shimada H., Kaji R., Mitani T., Matsumoto M., Toida K., Ishimura K., Shishido Y., Mak T.W., Fukui K.
      J. Biol. Chem. 279:41131-41140(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH APAF1.
    6. "Nucling mediates apoptosis by inhibiting expression of galectin-3 through interference with nuclear factor kappaB signalling."
      Liu L., Sakai T., Sano N., Fukui K.
      Biochem. J. 380:31-41(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH LGALS3, DISRUPTION PHENOTYPE.
    7. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUACA_MOUSE
    AccessioniPrimary (citable) accession number: Q8CGB3
    Secondary accession number(s): Q69ZG3, Q7TN77, Q8BJC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: April 4, 2006
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3