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Q8CG76

- ARK72_MOUSE

UniProt

Q8CG76 - ARK72_MOUSE

Protein

Aflatoxin B1 aldehyde reductase member 2

Gene

Akr7a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen By similarity.By similarity

    Catalytic activityi

    4-hydroxybutanoate + NADP+ = succinate semialdehyde + NADPH.1 Publication

    Enzyme regulationi

    Inhibited by citrate, succinate and tartrate.1 Publication

    Kineticsi

    1. KM=19.8 µM for succinic semialdehyde1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801NADP1 Publication
    Active sitei85 – 851Proton donor
    Sitei113 – 1131Lowers pKa of active site TyrBy similarity
    Binding sitei149 – 1491Substrate
    Binding sitei205 – 2051NADP1 Publication
    Binding sitei258 – 2581NADP1 Publication
    Binding sitei268 – 2681Substrate
    Binding sitei271 – 2711Substrate
    Binding sitei367 – 3671Substrate

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi179 – 1802NADP1 Publication
    Nucleotide bindingi234 – 24411NADP1 PublicationAdd
    BLAST
    Nucleotide bindingi326 – 3349NADP1 Publication

    GO - Molecular functioni

    1. alditol:NADP+ 1-oxidoreductase activity Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    SABIO-RKQ8CG76.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aflatoxin B1 aldehyde reductase member 2 (EC:1.1.1.n11)
    Alternative name(s):
    Succinic semialdehyde reductase
    Short name:
    SSA reductase
    Gene namesi
    Name:Akr7a2By similarity
    Synonyms:AfarImported, Akr7a5Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:107796. Akr7a5.

    Subcellular locationi

    Golgi apparatus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Aflatoxin B1 aldehyde reductase member 2PRO_0000070376Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei136 – 1361N6-acetyllysine1 Publication
    Modified residuei244 – 2441N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8CG76.
    PaxDbiQ8CG76.
    PRIDEiQ8CG76.

    2D gel databases

    REPRODUCTION-2DPAGEQ8CG76.

    PTM databases

    PhosphoSiteiQ8CG76.

    Expressioni

    Tissue specificityi

    Expressed in liver, kidney, testis and brain with low levels in skeletal muscle, spleen, heart and lung.1 Publication

    Gene expression databases

    BgeeiQ8CG76.
    GenevestigatoriQ8CG76.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiQ8CG76. 3 interactions.
    MINTiMINT-1869477.

    Structurei

    Secondary structure

    1
    367
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi47 – 515
    Turni56 – 583
    Helixi61 – 7313
    Beta strandi78 – 803
    Helixi85 – 884
    Helixi89 – 957
    Beta strandi109 – 1146
    Turni117 – 1204
    Helixi124 – 13714
    Beta strandi143 – 1486
    Helixi157 – 16913
    Beta strandi172 – 1809
    Helixi183 – 19614
    Beta strandi201 – 2077
    Helixi214 – 2163
    Helixi219 – 2268
    Beta strandi229 – 2335
    Helixi237 – 2426
    Helixi247 – 2504
    Turni251 – 2533
    Beta strandi260 – 2623
    Helixi265 – 2728
    Helixi275 – 29218
    Helixi293 – 2953
    Helixi299 – 30911
    Helixi315 – 3173
    Beta strandi320 – 3234
    Helixi328 – 33710
    Helixi345 – 35814
    Helixi359 – 3613

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2C91X-ray2.30A/B/C/D/E/F/G/H/I/J30-367[»]
    ProteinModelPortaliQ8CG76.
    SMRiQ8CG76. Positions 43-367.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8CG76.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0667.
    GeneTreeiENSGT00550000074567.
    HOGENOMiHOG000250286.
    HOVERGENiHBG050576.
    InParanoidiA2AMV3.
    KOiK15303.
    OMAiCTVKIAT.
    OrthoDBiEOG77127F.
    TreeFamiTF329173.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    SUPFAMiSSF51430. SSF51430. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8CG76-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRAASRAVG RAAVRSAQRS GTSVGRPLAM SRPPPPRAAS GAPLRPATVL    50
    GTMEMGRRMD ASASAASVRA FLERGHSELD TAFMYCDGQS ENILGGLGLG 100
    LGSGDCTVKI ATKANPWEGK SLKPDSIRSQ LETSLKRLQC PRVDLFYLHA 150
    PDHSTPVEET LRACHQLHQE GKFVELGLSN YASWEVAEIC TLCKSNGWIL 200
    PTVYQGMYNA TTRQVEAELL PCLRHFGLRF YAYNPLAGGL LTGKYKYEDK 250
    DGKQPVGRFF GNNWAETYRN RFWKEHHFEA IALVEKALQT TYGTNAPRMT 300
    SAALRWMYHH SQLQGTRGDA VILGMSSLEQ LEQNLAATEE GPLEPAVVEA 350
    FDQAWNMVAH ECPNYFR 367
    Length:367
    Mass (Da):40,612
    Last modified:July 27, 2011 - v3
    Checksum:i1123BD7028D3FBDF
    GO

    Sequence cautioni

    The sequence AAH31857.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAC81077.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAC81078.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51A → G in BAE25302. (PubMed:16141072)Curated
    Sequence conflicti9 – 91V → G in BAE25302. (PubMed:16141072)Curated
    Sequence conflicti12 – 121A → V in BAE25302. (PubMed:16141072)Curated
    Sequence conflicti17 – 171A → G in BAE25302. (PubMed:16141072)Curated
    Sequence conflicti127 – 1271I → V in AAH31857. (PubMed:15489334)Curated
    Sequence conflicti127 – 1271I → V in CAC81078. (PubMed:12879023)Curated
    Sequence conflicti162 – 1621R → C in AAO38437. (PubMed:12123834)Curated
    Sequence conflicti354 – 3541A → G in AAH31857. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK143203 mRNA. Translation: BAE25302.1.
    AL807811 Genomic DNA. Translation: CAM18543.1.
    BC031857 mRNA. Translation: AAH31857.1. Different initiation.
    AJ271800 mRNA. Translation: CAC81077.1. Different initiation.
    AJ271801 mRNA. Translation: CAC81078.1. Different initiation.
    AF525358 mRNA. Translation: AAO38437.2.
    BK000393 mRNA. Translation: DAA00087.1.
    CCDSiCCDS18844.1.
    RefSeqiNP_079613.3. NM_025337.3.
    UniGeneiMm.482154.

    Genome annotation databases

    EnsembliENSMUST00000073787; ENSMUSP00000073459; ENSMUSG00000028743.
    GeneIDi110198.
    KEGGimmu:110198.
    UCSCiuc008vmc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK143203 mRNA. Translation: BAE25302.1 .
    AL807811 Genomic DNA. Translation: CAM18543.1 .
    BC031857 mRNA. Translation: AAH31857.1 . Different initiation.
    AJ271800 mRNA. Translation: CAC81077.1 . Different initiation.
    AJ271801 mRNA. Translation: CAC81078.1 . Different initiation.
    AF525358 mRNA. Translation: AAO38437.2 .
    BK000393 mRNA. Translation: DAA00087.1 .
    CCDSi CCDS18844.1.
    RefSeqi NP_079613.3. NM_025337.3.
    UniGenei Mm.482154.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2C91 X-ray 2.30 A/B/C/D/E/F/G/H/I/J 30-367 [» ]
    ProteinModelPortali Q8CG76.
    SMRi Q8CG76. Positions 43-367.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8CG76. 3 interactions.
    MINTi MINT-1869477.

    PTM databases

    PhosphoSitei Q8CG76.

    2D gel databases

    REPRODUCTION-2DPAGE Q8CG76.

    Proteomic databases

    MaxQBi Q8CG76.
    PaxDbi Q8CG76.
    PRIDEi Q8CG76.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000073787 ; ENSMUSP00000073459 ; ENSMUSG00000028743 .
    GeneIDi 110198.
    KEGGi mmu:110198.
    UCSCi uc008vmc.2. mouse.

    Organism-specific databases

    CTDi 110198.
    MGIi MGI:107796. Akr7a5.

    Phylogenomic databases

    eggNOGi COG0667.
    GeneTreei ENSGT00550000074567.
    HOGENOMi HOG000250286.
    HOVERGENi HBG050576.
    InParanoidi A2AMV3.
    KOi K15303.
    OMAi CTVKIAT.
    OrthoDBi EOG77127F.
    TreeFami TF329173.

    Enzyme and pathway databases

    SABIO-RK Q8CG76.

    Miscellaneous databases

    EvolutionaryTracei Q8CG76.
    NextBioi 363519.
    PROi Q8CG76.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CG76.
    Genevestigatori Q8CG76.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51430. SSF51430. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-367.
      Strain: FVB/N.
      Tissue: LiverImported.
    4. "Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells."
      Praml C., Savelyeva L., Schwab M.
      Oncogene 22:4765-4773(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-367.
      Strain: BALB/cImported.
    5. "Characterisation of a novel mouse liver aldo-keto reductase AKR7A5."
      Hinshelwood A., McGarvie G., Ellis E.
      FEBS Lett. 523:213-218(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-367, TISSUE SPECIFICITY.
      Strain: CD-1.
      Tissue: Liver.
    6. Ellis E.M., Hinshelwood A., McGarvie G.
      Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 127 AND 162.
    7. "Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of aldo-keto reductase 7 family proteins."
      Kelly V.P., Sherratt P.J., Crouch D.H., Hayes J.D.
      Biochem. J. 366:847-861(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE STRUCTURE.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-244, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    10. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    11. "Crystal structure of mouse succinic semialdehyde reductase AKR7A5: structural basis for substrate specificity."
      Zhu X., Lapthorn A.J., Ellis E.M.
      Biochemistry 45:1562-1570(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 30-367 IN COMPLEX WITH NADP AND THE INHIBITOR TARTRATE, ENZYME REGULATION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiARK72_MOUSE
    AccessioniPrimary (citable) accession number: Q8CG76
    Secondary accession number(s): A2AMV3
    , Q3UPU2, Q8CG77, Q8JZQ8, Q9D157
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 108 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3