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Protein

Poly(ADP-ribose) glycohydrolase ARH3

Gene

Adprhl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Generates ADP-ribose from poly-(ADP-ribose), but does not hydrolyze ADP-ribose-arginine, -cysteine, -diphthamide, or -asparagine bonds. Due to catalytic inactivity of PARG mitochondrial isoforms, ARH3 is the only PAR hydrolyzing enzyme in mitochondria (By similarity).By similarity

Catalytic activityi

Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose.

Cofactori

Mg2+1 PublicationNote: Binds 2 magnesium ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Magnesium 21
Metal bindingi82Magnesium 11
Metal bindingi83Magnesium 11
Metal bindingi84Magnesium 11
Binding sitei260SubstrateBy similarity1
Metal bindingi320Magnesium 21
Metal bindingi322Magnesium 11
Metal bindingi322Magnesium 21
Metal bindingi323Magnesium 21

GO - Molecular functioni

GO - Biological processi

  • cellular response to superoxide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.143. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(ADP-ribose) glycohydrolase ARH3 (EC:3.2.1.143)
Alternative name(s):
ADP-ribosylhydrolase 3
[Protein ADP-ribosylarginine] hydrolase-like protein 2
Gene namesi
Name:Adprhl2
Synonyms:Arh3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2140364. Adprhl2.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication
  • Mitochondrion matrix By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33MitochondrionSequence analysisAdd BLAST33
ChainiPRO_000027761434 – 370Poly(ADP-ribose) glycohydrolase ARH3Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8CG72.
PaxDbiQ8CG72.
PeptideAtlasiQ8CG72.
PRIDEiQ8CG72.

PTM databases

PhosphoSitePlusiQ8CG72.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000042558.
CleanExiMM_ADPRHL2.
GenevisibleiQ8CG72. MM.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiQ8CG72. 1 interactor.
MINTiMINT-4383260.
STRINGi10090.ENSMUSP00000099677.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 43Combined sources19
Helixi44 – 46Combined sources3
Helixi54 – 62Combined sources9
Helixi83 – 98Combined sources16
Helixi103 – 116Combined sources14
Turni118 – 121Combined sources4
Helixi124 – 133Combined sources10
Helixi143 – 146Combined sources4
Helixi148 – 151Combined sources4
Helixi158 – 161Combined sources4
Helixi164 – 169Combined sources6
Helixi173 – 185Combined sources13
Helixi191 – 209Combined sources19
Helixi214 – 228Combined sources15
Helixi232 – 239Combined sources8
Turni240 – 242Combined sources3
Helixi247 – 259Combined sources13
Helixi266 – 273Combined sources8
Beta strandi276 – 278Combined sources3
Helixi279 – 281Combined sources3
Helixi283 – 292Combined sources10
Helixi306 – 315Combined sources10
Helixi321 – 336Combined sources16
Helixi338 – 340Combined sources3
Helixi343 – 346Combined sources4
Helixi352 – 366Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QTYX-ray1.80A/B25-369[»]
ProteinModelPortaliQ8CG72.
SMRiQ8CG72.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CG72.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 11Poly-Ala10
Compositional biasi16 – 19Poly-Gly4

Sequence similaritiesi

Belongs to the ADP-ribosylglycohydrolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IGSE. Eukaryota.
COG1397. LUCA.
GeneTreeiENSGT00390000015369.
HOGENOMiHOG000225333.
HOVERGENiHBG080863.
InParanoidiQ8CG72.
KOiK11687.
OMAiKCRDVYE.
OrthoDBiEOG091G0DA4.
PhylomeDBiQ8CG72.
TreeFamiTF324754.

Family and domain databases

InterProiIPR005502. Ribosyl_crysJ1.
[Graphical view]
PfamiPF03747. ADP_ribosyl_GH. 1 hit.
[Graphical view]
SUPFAMiSSF101478. SSF101478. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CG72-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVAAAAAAT AMSAAGGGGA SAARSISRFR GCLAGALLGD CVGAVYEAHD
60 70 80 90 100
TVSLASVLSH VESLEPDPGT PGSARTETLY YTDDTAMTRA LVQSLLAKEA
110 120 130 140 150
FDEVDMAHRF AQEYKKDPDR GYGAGVITVF KKLLNPKCRD VYEPARAQFN
160 170 180 190 200
GKGSYGNGGA MRVAGISLAY SSVQDVQKFA RLSAQLTHAS SLGYNGAILQ
210 220 230 240 250
ALAVHLALQG VSSSEHFLEQ LLGHMEELEG DAQSVLDAKE LGMEERPYSS
260 270 280 290 300
RLKKVGELLD QDVVSREEVV SELGNGIAAF ESVPTAIYCF LRCMEPHPEI
310 320 330 340 350
PSTFNSLQRT LIYSISLGGD TDTIATMAGA IAGAYYGMEQ VPESWQQSCE
360 370
GFEETDVLAQ SLHRVFQESS
Length:370
Mass (Da):39,414
Last modified:March 1, 2003 - v1
Checksum:i3251113C67432A8D
GO
Isoform 2 (identifier: Q8CG72-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.

Show »
Length:284
Mass (Da):31,043
Checksum:i01B66A15E6625CBF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77E → Q in AAH23177 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0230371 – 86Missing in isoform 2. 1 PublicationAdd BLAST86

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ427296 mRNA. Translation: CAD20317.1.
AK143583 mRNA. Translation: BAE25451.1.
AK147034 mRNA. Translation: BAE27626.1.
AK169070 mRNA. Translation: BAE40857.1.
AK171780 mRNA. Translation: BAE42660.1.
AL606976 Genomic DNA. Translation: CAM45877.1.
BC010639 mRNA. Translation: AAH10639.1.
BC023177 mRNA. Translation: AAH23177.2.
BC045203 mRNA. Translation: AAH45203.1.
CCDSiCCDS18650.1. [Q8CG72-1]
RefSeqiNP_598644.1. NM_133883.2. [Q8CG72-1]
UniGeneiMm.11285.

Genome annotation databases

EnsembliENSMUST00000102617; ENSMUSP00000099677; ENSMUSG00000042558. [Q8CG72-1]
GeneIDi100206.
KEGGimmu:100206.
UCSCiuc008ute.1. mouse. [Q8CG72-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ427296 mRNA. Translation: CAD20317.1.
AK143583 mRNA. Translation: BAE25451.1.
AK147034 mRNA. Translation: BAE27626.1.
AK169070 mRNA. Translation: BAE40857.1.
AK171780 mRNA. Translation: BAE42660.1.
AL606976 Genomic DNA. Translation: CAM45877.1.
BC010639 mRNA. Translation: AAH10639.1.
BC023177 mRNA. Translation: AAH23177.2.
BC045203 mRNA. Translation: AAH45203.1.
CCDSiCCDS18650.1. [Q8CG72-1]
RefSeqiNP_598644.1. NM_133883.2. [Q8CG72-1]
UniGeneiMm.11285.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QTYX-ray1.80A/B25-369[»]
ProteinModelPortaliQ8CG72.
SMRiQ8CG72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CG72. 1 interactor.
MINTiMINT-4383260.
STRINGi10090.ENSMUSP00000099677.

PTM databases

PhosphoSitePlusiQ8CG72.

Proteomic databases

EPDiQ8CG72.
PaxDbiQ8CG72.
PeptideAtlasiQ8CG72.
PRIDEiQ8CG72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102617; ENSMUSP00000099677; ENSMUSG00000042558. [Q8CG72-1]
GeneIDi100206.
KEGGimmu:100206.
UCSCiuc008ute.1. mouse. [Q8CG72-1]

Organism-specific databases

CTDi54936.
MGIiMGI:2140364. Adprhl2.

Phylogenomic databases

eggNOGiENOG410IGSE. Eukaryota.
COG1397. LUCA.
GeneTreeiENSGT00390000015369.
HOGENOMiHOG000225333.
HOVERGENiHBG080863.
InParanoidiQ8CG72.
KOiK11687.
OMAiKCRDVYE.
OrthoDBiEOG091G0DA4.
PhylomeDBiQ8CG72.
TreeFamiTF324754.

Enzyme and pathway databases

BRENDAi3.2.1.143. 3474.

Miscellaneous databases

EvolutionaryTraceiQ8CG72.
PROiQ8CG72.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042558.
CleanExiMM_ADPRHL2.
GenevisibleiQ8CG72. MM.

Family and domain databases

InterProiIPR005502. Ribosyl_crysJ1.
[Graphical view]
PfamiPF03747. ADP_ribosyl_GH. 1 hit.
[Graphical view]
SUPFAMiSSF101478. SSF101478. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARHL2_MOUSE
AccessioniPrimary (citable) accession number: Q8CG72
Secondary accession number(s): A3KFY3
, Q80UW9, Q8R575, Q921U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.