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Q8CG71 (P3H2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolyl 3-hydroxylase 2
EC=1.14.11.7
Alternative name(s):
Leprecan-like protein 1
Gene names
Name:Leprel1
Synonyms:P3h2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length703 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Iron By similarity.

Ascorbate By similarity.

Subcellular location

Endoplasmic reticulum. Golgi apparatus By similarity.

Sequence similarities

Belongs to the leprecan family.

Contains 1 Fe2OG dioxygenase domain.

Contains 4 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 703682Prolyl 3-hydroxylase 2
PRO_0000240357

Regions

Repeat42 – 7534TPR 1
Repeat144 – 17734TPR 2
Repeat205 – 23834TPR 3
Repeat301 – 33434TPR 4
Domain552 – 666115Fe2OG dioxygenase
Motif700 – 7034Prevents secretion from ER Potential

Sites

Active site6571 By similarity
Metal binding5751Iron
Metal binding5771Iron
Metal binding6471Iron

Amino acid modifications

Glycosylation4441N-linked (GlcNAc...) Potential
Glycosylation5441N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4971P → A in BAC36342. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8CG71 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 1F42F9B9938573E4

FASTA70380,154
        10         20         30         40         50         60 
MRESTWVSLL LLLLLPTPQR GGPQDGRRSP EPEPERGPLQ PFDLLYASGV AAYYSGDYER 

        70         80         90        100        110        120 
AVRDLEAALS SHRRLRDIRT RCARHCAARR PLAPPGAGPG AELPFFRAVL ERARCSRSCQ 

       130        140        150        160        170        180 
SQRLGGPASR HRVSEDVRSD FQRRVPYNYL QRAYIKLNQL EKAMEAAHTF FMANPEHMEM 

       190        200        210        220        230        240 
QQDLEDYKAT ARVEAPLLDR EAKPHLESYN AGVKHYEADD FESAIKYFEQ ALREYFNEDM 

       250        260        270        280        290        300 
ECRALCEGPQ RFEEYEYLGY KGGLYEAIAD HYMQVLVCQH ECVRELATRP GRLSPIENFL 

       310        320        330        340        350        360 
PLHYDYLQFA YYRVGEYVKA LECAKAYLMF HPDNEDVLDN VDFYESLLDD STDPASIEAR 

       370        380        390        400        410        420 
EDLTAFVKRH KLEAELIKLA AEGLGFSYAE PNYWISYGGR QDENRVPSGV NMDGAEVHGL 

       430        440        450        460        470        480 
SMGKKSPPKI GRDLREGGPL LYENITFVYN SEQLNGTQRV LLDNVLSQEQ CRELHSVANG 

       490        500        510        520        530        540 
IMLVGDGYRG KTSPHTPNEK FEGATVLKAL KFGYEGRVPL KSARLFYDIS EKARKIVESY 

       550        560        570        580        590        600 
FMLNSTLYFS YTHMVCRTAL SGQQDRRNDL SHPIHADNCL LDPEANECWK EPPAYTFRDY 

       610        620        630        640        650        660 
SALLYMNDDF DGGEFIFTEM DAKTVTASIK PKCGRMISFS SGGENPHGVK AVTRGQRCAV 

       670        680        690        700 
ALWFTLDPLY RELERIQADE VIAILDQEQR GKHGLNINPK DEL

« Hide

References

« Hide 'large scale' references
[1]"LEPREL1, a novel ER and Golgi resident member of the Leprecan family."
Jaernum S., Kjellman C., Darabi A., Nilsson I., Edvardsen K., Aaman P.
Biochem. Biophys. Res. Commun. 317:342-351(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 295-703.
Strain: C57BL/6J.
Tissue: Head.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ430350 mRNA. Translation: CAD23038.1.
AK076434 mRNA. Translation: BAC36342.1.
IPIIPI00229428.
RefSeqNP_775555.1. NM_173379.2.
UniGeneMm.483044.

3D structure databases

ProteinModelPortalQ8CG71.
SMRQ8CG71. Positions 38-72, 151-184.
ModBaseSearch...

PTM databases

PhosphoSiteQ8CG71.

Proteomic databases

PaxDbQ8CG71.
PRIDEQ8CG71.

Protocols and materials databases

DNASU210530.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039990; ENSMUSP00000038056; ENSMUSG00000038168.
GeneID210530.
KEGGmmu:210530.
UCSCuc007yuy.1. mouse.

Organism-specific databases

CTD55214.
MGIMGI:2146663. Leprel1.

Phylogenomic databases

eggNOGNOG269251.
GeneTreeENSGT00550000074573.
HOGENOMHOG000231087.
HOVERGENHBG053224.
InParanoidQ8CG71.
OMAQGKHELN.
OrthoDBEOG4WSW97.

Gene expression databases

BgeeQ8CG71.
CleanExMM_LEPREL1.
GenevestigatorQ8CG71.
GermOnlineENSMUSG00000038168. Mus musculus.

Family and domain databases

Gene3D1.25.40.10. 4 hits.
InterProIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical.
IPR013105. TPR_2.
[Graphical view]
PfamPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF07719. TPR_2. 2 hits.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. False negative.
PS50293. TPR_REGION. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio372972.
SOURCESearch...

Entry information

Entry nameP3H2_MOUSE
AccessionPrimary (citable) accession number: Q8CG71
Secondary accession number(s): Q8C673
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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