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Protein

Prolyl 3-hydroxylase 2

Gene

Leprel1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V.By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi575 – 5751Iron
Metal bindingi577 – 5771Iron
Metal bindingi647 – 6471Iron
Active sitei657 – 6571By similarity

GO - Molecular functioni

  • iron ion binding Source: InterPro
  • L-ascorbic acid binding Source: UniProtKB-KW
  • procollagen-proline 3-dioxygenase activity Source: UniProtKB

GO - Biological processi

  • collagen metabolic process Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • peptidyl-proline hydroxylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.
ReactomeiREACT_285754. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 2 (EC:1.14.11.7)
Alternative name(s):
Leprecan-like protein 1
Gene namesi
Name:Leprel1
Synonyms:P3h2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2146663. Leprel1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 703682Prolyl 3-hydroxylase 2PRO_0000240357Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8CG71.
PaxDbiQ8CG71.
PRIDEiQ8CG71.

PTM databases

PhosphoSiteiQ8CG71.

Expressioni

Gene expression databases

BgeeiQ8CG71.
CleanExiMM_LEPREL1.
GenevisibleiQ8CG71. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038056.

Structurei

3D structure databases

ProteinModelPortaliQ8CG71.
SMRiQ8CG71. Positions 150-184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 7534TPR 1Add
BLAST
Repeati144 – 17734TPR 2Add
BLAST
Repeati205 – 23834TPR 3Add
BLAST
Repeati301 – 33434TPR 4Add
BLAST
Domaini552 – 666115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi700 – 7034Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiNOG269251.
GeneTreeiENSGT00550000074573.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ8CG71.
OMAiQGKHELN.
OrthoDBiEOG7BZVSS.
PhylomeDBiQ8CG71.
TreeFamiTF320837.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF07719. TPR_2. 2 hits.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRESTWVSLL LLLLLPTPQR GGPQDGRRSP EPEPERGPLQ PFDLLYASGV
60 70 80 90 100
AAYYSGDYER AVRDLEAALS SHRRLRDIRT RCARHCAARR PLAPPGAGPG
110 120 130 140 150
AELPFFRAVL ERARCSRSCQ SQRLGGPASR HRVSEDVRSD FQRRVPYNYL
160 170 180 190 200
QRAYIKLNQL EKAMEAAHTF FMANPEHMEM QQDLEDYKAT ARVEAPLLDR
210 220 230 240 250
EAKPHLESYN AGVKHYEADD FESAIKYFEQ ALREYFNEDM ECRALCEGPQ
260 270 280 290 300
RFEEYEYLGY KGGLYEAIAD HYMQVLVCQH ECVRELATRP GRLSPIENFL
310 320 330 340 350
PLHYDYLQFA YYRVGEYVKA LECAKAYLMF HPDNEDVLDN VDFYESLLDD
360 370 380 390 400
STDPASIEAR EDLTAFVKRH KLEAELIKLA AEGLGFSYAE PNYWISYGGR
410 420 430 440 450
QDENRVPSGV NMDGAEVHGL SMGKKSPPKI GRDLREGGPL LYENITFVYN
460 470 480 490 500
SEQLNGTQRV LLDNVLSQEQ CRELHSVANG IMLVGDGYRG KTSPHTPNEK
510 520 530 540 550
FEGATVLKAL KFGYEGRVPL KSARLFYDIS EKARKIVESY FMLNSTLYFS
560 570 580 590 600
YTHMVCRTAL SGQQDRRNDL SHPIHADNCL LDPEANECWK EPPAYTFRDY
610 620 630 640 650
SALLYMNDDF DGGEFIFTEM DAKTVTASIK PKCGRMISFS SGGENPHGVK
660 670 680 690 700
AVTRGQRCAV ALWFTLDPLY RELERIQADE VIAILDQEQR GKHGLNINPK

DEL
Length:703
Mass (Da):80,154
Last modified:March 1, 2003 - v1
Checksum:i1F42F9B9938573E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti497 – 4971P → A in BAC36342 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ430350 mRNA. Translation: CAD23038.1.
AK076434 mRNA. Translation: BAC36342.1.
CCDSiCCDS28086.1.
RefSeqiNP_775555.1. NM_173379.2.
UniGeneiMm.326869.

Genome annotation databases

EnsembliENSMUST00000039990; ENSMUSP00000038056; ENSMUSG00000038168.
GeneIDi210530.
KEGGimmu:210530.
UCSCiuc007yuy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ430350 mRNA. Translation: CAD23038.1.
AK076434 mRNA. Translation: BAC36342.1.
CCDSiCCDS28086.1.
RefSeqiNP_775555.1. NM_173379.2.
UniGeneiMm.326869.

3D structure databases

ProteinModelPortaliQ8CG71.
SMRiQ8CG71. Positions 150-184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038056.

PTM databases

PhosphoSiteiQ8CG71.

Proteomic databases

MaxQBiQ8CG71.
PaxDbiQ8CG71.
PRIDEiQ8CG71.

Protocols and materials databases

DNASUi210530.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039990; ENSMUSP00000038056; ENSMUSG00000038168.
GeneIDi210530.
KEGGimmu:210530.
UCSCiuc007yuy.1. mouse.

Organism-specific databases

CTDi55214.
MGIiMGI:2146663. Leprel1.

Phylogenomic databases

eggNOGiNOG269251.
GeneTreeiENSGT00550000074573.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ8CG71.
OMAiQGKHELN.
OrthoDBiEOG7BZVSS.
PhylomeDBiQ8CG71.
TreeFamiTF320837.

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.
ReactomeiREACT_285754. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

NextBioi372972.
PROiQ8CG71.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CG71.
CleanExiMM_LEPREL1.
GenevisibleiQ8CG71. MM.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF07719. TPR_2. 2 hits.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "LEPREL1, a novel ER and Golgi resident member of the Leprecan family."
    Jaernum S., Kjellman C., Darabi A., Nilsson I., Edvardsen K., Aaman P.
    Biochem. Biophys. Res. Commun. 317:342-351(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 295-703.
    Strain: C57BL/6J.
    Tissue: Head.

Entry informationi

Entry nameiP3H2_MOUSE
AccessioniPrimary (citable) accession number: Q8CG71
Secondary accession number(s): Q8C673
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: June 24, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.