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Q8CG71

- P3H2_MOUSE

UniProt

Q8CG71 - P3H2_MOUSE

Protein

Prolyl 3-hydroxylase 2

Gene

Leprel1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V.By similarity

    Catalytic activityi

    L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

    Cofactori

    Iron.By similarity
    Ascorbate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi575 – 5751Iron
    Metal bindingi577 – 5771Iron
    Metal bindingi647 – 6471Iron
    Active sitei657 – 6571By similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. L-ascorbic acid binding Source: UniProtKB-KW
    3. procollagen-proline 3-dioxygenase activity Source: UniProtKB

    GO - Biological processi

    1. collagen metabolic process Source: UniProtKB
    2. negative regulation of cell proliferation Source: UniProtKB
    3. peptidyl-proline hydroxylation Source: UniProtKB

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding, Vitamin C

    Enzyme and pathway databases

    ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prolyl 3-hydroxylase 2 (EC:1.14.11.7)
    Alternative name(s):
    Leprecan-like protein 1
    Gene namesi
    Name:Leprel1
    Synonyms:P3h2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:2146663. Leprel1.

    Subcellular locationi

    GO - Cellular componenti

    1. basement membrane Source: UniProtKB
    2. endoplasmic reticulum Source: UniProtKB
    3. Golgi apparatus Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 703682Prolyl 3-hydroxylase 2PRO_0000240357Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8CG71.
    PRIDEiQ8CG71.

    PTM databases

    PhosphoSiteiQ8CG71.

    Expressioni

    Gene expression databases

    BgeeiQ8CG71.
    CleanExiMM_LEPREL1.
    GenevestigatoriQ8CG71.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CG71.
    SMRiQ8CG71. Positions 145-193, 306-349.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati42 – 7534TPR 1Add
    BLAST
    Repeati144 – 17734TPR 2Add
    BLAST
    Repeati205 – 23834TPR 3Add
    BLAST
    Repeati301 – 33434TPR 4Add
    BLAST
    Domaini552 – 666115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi700 – 7034Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the leprecan family.Curated
    Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
    Contains 4 TPR repeats.Curated

    Keywords - Domaini

    Repeat, Signal, TPR repeat

    Phylogenomic databases

    eggNOGiNOG269251.
    GeneTreeiENSGT00550000074573.
    HOGENOMiHOG000231087.
    HOVERGENiHBG053224.
    InParanoidiQ8CG71.
    OMAiQGKHELN.
    OrthoDBiEOG7BZVSS.
    PhylomeDBiQ8CG71.
    TreeFamiTF320837.

    Family and domain databases

    Gene3Di1.25.40.10. 4 hits.
    InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
    IPR006620. Pro_4_hyd_alph.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    [Graphical view]
    PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
    PF07719. TPR_2. 2 hits.
    [Graphical view]
    SMARTiSM00702. P4Hc. 1 hit.
    [Graphical view]
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS51471. FE2OG_OXY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CG71-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRESTWVSLL LLLLLPTPQR GGPQDGRRSP EPEPERGPLQ PFDLLYASGV    50
    AAYYSGDYER AVRDLEAALS SHRRLRDIRT RCARHCAARR PLAPPGAGPG 100
    AELPFFRAVL ERARCSRSCQ SQRLGGPASR HRVSEDVRSD FQRRVPYNYL 150
    QRAYIKLNQL EKAMEAAHTF FMANPEHMEM QQDLEDYKAT ARVEAPLLDR 200
    EAKPHLESYN AGVKHYEADD FESAIKYFEQ ALREYFNEDM ECRALCEGPQ 250
    RFEEYEYLGY KGGLYEAIAD HYMQVLVCQH ECVRELATRP GRLSPIENFL 300
    PLHYDYLQFA YYRVGEYVKA LECAKAYLMF HPDNEDVLDN VDFYESLLDD 350
    STDPASIEAR EDLTAFVKRH KLEAELIKLA AEGLGFSYAE PNYWISYGGR 400
    QDENRVPSGV NMDGAEVHGL SMGKKSPPKI GRDLREGGPL LYENITFVYN 450
    SEQLNGTQRV LLDNVLSQEQ CRELHSVANG IMLVGDGYRG KTSPHTPNEK 500
    FEGATVLKAL KFGYEGRVPL KSARLFYDIS EKARKIVESY FMLNSTLYFS 550
    YTHMVCRTAL SGQQDRRNDL SHPIHADNCL LDPEANECWK EPPAYTFRDY 600
    SALLYMNDDF DGGEFIFTEM DAKTVTASIK PKCGRMISFS SGGENPHGVK 650
    AVTRGQRCAV ALWFTLDPLY RELERIQADE VIAILDQEQR GKHGLNINPK 700
    DEL 703
    Length:703
    Mass (Da):80,154
    Last modified:March 1, 2003 - v1
    Checksum:i1F42F9B9938573E4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti497 – 4971P → A in BAC36342. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ430350 mRNA. Translation: CAD23038.1.
    AK076434 mRNA. Translation: BAC36342.1.
    CCDSiCCDS28086.1.
    RefSeqiNP_775555.1. NM_173379.2.
    UniGeneiMm.326869.

    Genome annotation databases

    EnsembliENSMUST00000039990; ENSMUSP00000038056; ENSMUSG00000038168.
    GeneIDi210530.
    KEGGimmu:210530.
    UCSCiuc007yuy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ430350 mRNA. Translation: CAD23038.1 .
    AK076434 mRNA. Translation: BAC36342.1 .
    CCDSi CCDS28086.1.
    RefSeqi NP_775555.1. NM_173379.2.
    UniGenei Mm.326869.

    3D structure databases

    ProteinModelPortali Q8CG71.
    SMRi Q8CG71. Positions 145-193, 306-349.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8CG71.

    Proteomic databases

    PaxDbi Q8CG71.
    PRIDEi Q8CG71.

    Protocols and materials databases

    DNASUi 210530.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039990 ; ENSMUSP00000038056 ; ENSMUSG00000038168 .
    GeneIDi 210530.
    KEGGi mmu:210530.
    UCSCi uc007yuy.1. mouse.

    Organism-specific databases

    CTDi 55214.
    MGIi MGI:2146663. Leprel1.

    Phylogenomic databases

    eggNOGi NOG269251.
    GeneTreei ENSGT00550000074573.
    HOGENOMi HOG000231087.
    HOVERGENi HBG053224.
    InParanoidi Q8CG71.
    OMAi QGKHELN.
    OrthoDBi EOG7BZVSS.
    PhylomeDBi Q8CG71.
    TreeFami TF320837.

    Enzyme and pathway databases

    Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.

    Miscellaneous databases

    NextBioi 372972.
    PROi Q8CG71.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CG71.
    CleanExi MM_LEPREL1.
    Genevestigatori Q8CG71.

    Family and domain databases

    Gene3Di 1.25.40.10. 4 hits.
    InterProi IPR005123. Oxoglu/Fe-dep_dioxygenase.
    IPR006620. Pro_4_hyd_alph.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    [Graphical view ]
    Pfami PF13640. 2OG-FeII_Oxy_3. 1 hit.
    PF07719. TPR_2. 2 hits.
    [Graphical view ]
    SMARTi SM00702. P4Hc. 1 hit.
    [Graphical view ]
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS51471. FE2OG_OXY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LEPREL1, a novel ER and Golgi resident member of the Leprecan family."
      Jaernum S., Kjellman C., Darabi A., Nilsson I., Edvardsen K., Aaman P.
      Biochem. Biophys. Res. Commun. 317:342-351(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 295-703.
      Strain: C57BL/6J.
      Tissue: Head.

    Entry informationi

    Entry nameiP3H2_MOUSE
    AccessioniPrimary (citable) accession number: Q8CG71
    Secondary accession number(s): Q8C673
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3