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Q8CG70 (P3H3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Prolyl 3-hydroxylase 3
EC=1.14.11.7
Alternative name(s):
Leprecan-like protein 2
Protein B
Gene names
Name:Leprel2
Synonyms:P3h3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Iron By similarity.

Ascorbate By similarity.

Subcellular location

Endoplasmic reticulum By similarity.

Sequence similarities

Belongs to the leprecan family.

Contains 1 Fe2OG dioxygenase domain.

Contains 4 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 732713Prolyl 3-hydroxylase 3
PRO_0000240361

Regions

Repeat39 – 7234TPR 1
Repeat152 – 18534TPR 2
Repeat214 – 24734TPR 3
Repeat312 – 34534TPR 4
Domain557 – 671115Fe2OG dioxygenase
Coiled coil674 – 70330 Potential
Motif729 – 7324Prevents secretion from ER Potential
Compositional bias14 – 3926Pro-rich
Compositional bias689 – 6979Poly-Glu

Sites

Active site6621 By similarity
Metal binding5801Iron
Metal binding5821Iron
Metal binding6521Iron

Amino acid modifications

Glycosylation3271N-linked (GlcNAc...) Potential
Glycosylation4581N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8CG70 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: BD54733B60F1687A

FASTA73281,701
        10         20         30         40         50         60 
MLRLLRLLLL LLLPPPGSPE PPEPPGLAQL SPGSPPQAPD LLYADGLRAY SAGAWAPAVA 

        70         80         90        100        110        120 
LLREALRSRA ALGRARQECG ASCAAEPGAA LPSQLLGAPH PVSGPGVWEP LLLRATLRRA 

       130        140        150        160        170        180 
ECLTQCAVRR LGPGGAARLR VGSALRDAFR RREPYNYLQR AYYQLKKLDL AASAAHTFFV 

       190        200        210        220        230        240 
ANPTHLQMRE DMAKYRRMSA IRPQSFRDLV TPLYWAAYDT GLELLEQREA ALALPQLEEA 

       250        260        270        280        290        300 
LQGSLAHMES CRAACEGPEE HQGAEEEGEG SQGGLYEAIA GHWIRVLQCR QHCVADTATR 

       310        320        330        340        350        360 
PGRSFPVQDF LLSQLRRLHE AYAQVGNMSQ AMENVLSVLL FYPEDEAAKK ALNQYQTQLG 

       370        380        390        400        410        420 
EPRPDLGPRE DIQRFILRSL GEKRQLYYAM EHLGTSFKDP DSWTPEALIP KALRERLRED 

       430        440        450        460        470        480 
QEKKPWDHQP PQQKPLAHWK DALLMEGVTL TQDAQQLNGS ERAVLDGLLT SAECGVLLQL 

       490        500        510        520        530        540 
AKDAAQAGAR SGYRGRRSPH SPHERFEGLT VLKAAQLARA GTVGRPGAKL LLEVSERVRT 

       550        560        570        580        590        600 
LTQAYFSPER PLHLSFTHLV CRSAIEGEQE QRMDLSHPVH ADNCVLDPDT GECWREPPAY 

       610        620        630        640        650        660 
TYRDYSGLLY LNDDFKGGDL FFTQPNALTV TAQVRPRCGR LVAFSSGGEN PHGVWAVTRG 

       670        680        690        700        710        720 
RRCALALWHT WAPEHSEQEW TEAKELLQEE EEEEEEEDIL SRDPSPEPPS HKLQRVQEKA 

       730 
GKPRRVRVRE EL

« Hide

References

[1]"LEPREL1, a novel ER and Golgi resident member of the Leprecan family."
Jaernum S., Kjellman C., Darabi A., Nilsson I., Edvardsen K., Aaman P.
Biochem. Biophys. Res. Commun. 317:342-351(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
[2]"Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes."
Vranka J.A., Sakai L.Y., Bachinger H.P.
J. Biol. Chem. 279:23615-23621(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 139-732.
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 165-732.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ441086 mRNA. Translation: CAD29580.1.
AY463530 mRNA. Translation: AAS45239.1.
BC003726 mRNA. Translation: AAH03726.1.
BC016431 mRNA. Translation: AAH16431.1.
AC002397 Genomic DNA. Translation: AAC36012.1.
IPIIPI00407527.
RefSeqNP_038562.2. NM_013534.4.
UniGeneMm.35708.

3D structure databases

ProteinModelPortalQ8CG70.
SMRQ8CG70. Positions 316-355.
ModBaseSearch...

PTM databases

PhosphoSiteQ8CG70.

Proteomic databases

PaxDbQ8CG70.
PRIDEQ8CG70.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191.
GeneID14789.
KEGGmmu:14789.
UCSCuc009dsf.1. mouse.

Organism-specific databases

CTD10536.
MGIMGI:1315208. Leprel2.

Phylogenomic databases

eggNOGNOG269251.
GeneTreeENSGT00550000074573.
HOGENOMHOG000231087.
HOVERGENHBG053224.
InParanoidQ8CG70.
OMARAYYQLK.
OrthoDBEOG4VX24V.

Gene expression databases

ArrayExpressQ8CG70.
BgeeQ8CG70.
CleanExMM_LEPREL2.
GenevestigatorQ8CG70.
GermOnlineENSMUSG00000023191. Mus musculus.

Family and domain databases

Gene3D1.25.40.10. 3 hits.
InterProIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. False negative.
PS50293. TPR_REGION. False negative.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSLEPREL2. mouse.
NextBio286923.
SOURCESearch...

Entry information

Entry nameP3H3_MOUSE
AccessionPrimary (citable) accession number: Q8CG70
Secondary accession number(s): O88836
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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