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Protein

Prolyl 3-hydroxylase 3

Gene

Leprel2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi580 – 5801Iron
Metal bindingi582 – 5821Iron
Metal bindingi652 – 6521Iron
Active sitei662 – 6621By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. L-ascorbic acid binding Source: UniProtKB-KW
  3. procollagen-proline 3-dioxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. negative regulation of cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 3 (EC:1.14.11.7)
Alternative name(s):
Leprecan-like protein 2
Protein B
Gene namesi
Name:Leprel2
Synonyms:P3h3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1315208. Leprel2.

Subcellular locationi

Endoplasmic reticulum PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 732713Prolyl 3-hydroxylase 3PRO_0000240361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8CG70.
PaxDbiQ8CG70.
PRIDEiQ8CG70.

PTM databases

PhosphoSiteiQ8CG70.

Expressioni

Gene expression databases

BgeeiQ8CG70.
CleanExiMM_LEPREL2.
ExpressionAtlasiQ8CG70. baseline and differential.
GenevestigatoriQ8CG70.

Structurei

3D structure databases

ProteinModelPortaliQ8CG70.
SMRiQ8CG70. Positions 215-242, 321-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati39 – 7234TPR 1Add
BLAST
Repeati152 – 18534TPR 2Add
BLAST
Repeati214 – 24734TPR 3Add
BLAST
Repeati312 – 34534TPR 4Add
BLAST
Domaini557 – 671115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili674 – 70330Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi729 – 7324Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 3926Pro-richAdd
BLAST
Compositional biasi689 – 6979Poly-Glu

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiNOG269251.
GeneTreeiENSGT00550000074573.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ8CG70.
OMAiRAYYQLK.
OrthoDBiEOG7BZVSS.
PhylomeDBiQ8CG70.
TreeFamiTF320837.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLLRLLLL LLLPPPGSPE PPEPPGLAQL SPGSPPQAPD LLYADGLRAY
60 70 80 90 100
SAGAWAPAVA LLREALRSRA ALGRARQECG ASCAAEPGAA LPSQLLGAPH
110 120 130 140 150
PVSGPGVWEP LLLRATLRRA ECLTQCAVRR LGPGGAARLR VGSALRDAFR
160 170 180 190 200
RREPYNYLQR AYYQLKKLDL AASAAHTFFV ANPTHLQMRE DMAKYRRMSA
210 220 230 240 250
IRPQSFRDLV TPLYWAAYDT GLELLEQREA ALALPQLEEA LQGSLAHMES
260 270 280 290 300
CRAACEGPEE HQGAEEEGEG SQGGLYEAIA GHWIRVLQCR QHCVADTATR
310 320 330 340 350
PGRSFPVQDF LLSQLRRLHE AYAQVGNMSQ AMENVLSVLL FYPEDEAAKK
360 370 380 390 400
ALNQYQTQLG EPRPDLGPRE DIQRFILRSL GEKRQLYYAM EHLGTSFKDP
410 420 430 440 450
DSWTPEALIP KALRERLRED QEKKPWDHQP PQQKPLAHWK DALLMEGVTL
460 470 480 490 500
TQDAQQLNGS ERAVLDGLLT SAECGVLLQL AKDAAQAGAR SGYRGRRSPH
510 520 530 540 550
SPHERFEGLT VLKAAQLARA GTVGRPGAKL LLEVSERVRT LTQAYFSPER
560 570 580 590 600
PLHLSFTHLV CRSAIEGEQE QRMDLSHPVH ADNCVLDPDT GECWREPPAY
610 620 630 640 650
TYRDYSGLLY LNDDFKGGDL FFTQPNALTV TAQVRPRCGR LVAFSSGGEN
660 670 680 690 700
PHGVWAVTRG RRCALALWHT WAPEHSEQEW TEAKELLQEE EEEEEEEDIL
710 720 730
SRDPSPEPPS HKLQRVQEKA GKPRRVRVRE EL
Length:732
Mass (Da):81,701
Last modified:March 1, 2003 - v1
Checksum:iBD54733B60F1687A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ441086 mRNA. Translation: CAD29580.1.
AY463530 mRNA. Translation: AAS45239.1.
BC003726 mRNA. Translation: AAH03726.1.
BC016431 mRNA. Translation: AAH16431.1.
AC002397 Genomic DNA. Translation: AAC36012.1.
CCDSiCCDS20533.1.
RefSeqiNP_038562.2. NM_013534.4.
UniGeneiMm.35708.

Genome annotation databases

EnsembliENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191.
GeneIDi14789.
KEGGimmu:14789.
UCSCiuc009dsf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ441086 mRNA. Translation: CAD29580.1.
AY463530 mRNA. Translation: AAS45239.1.
BC003726 mRNA. Translation: AAH03726.1.
BC016431 mRNA. Translation: AAH16431.1.
AC002397 Genomic DNA. Translation: AAC36012.1.
CCDSiCCDS20533.1.
RefSeqiNP_038562.2. NM_013534.4.
UniGeneiMm.35708.

3D structure databases

ProteinModelPortaliQ8CG70.
SMRiQ8CG70. Positions 215-242, 321-353.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8CG70.

Proteomic databases

MaxQBiQ8CG70.
PaxDbiQ8CG70.
PRIDEiQ8CG70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191.
GeneIDi14789.
KEGGimmu:14789.
UCSCiuc009dsf.1. mouse.

Organism-specific databases

CTDi10536.
MGIiMGI:1315208. Leprel2.

Phylogenomic databases

eggNOGiNOG269251.
GeneTreeiENSGT00550000074573.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ8CG70.
OMAiRAYYQLK.
OrthoDBiEOG7BZVSS.
PhylomeDBiQ8CG70.
TreeFamiTF320837.

Enzyme and pathway databases

ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

ChiTaRSiLeprel2. mouse.
NextBioi286923.
PROiQ8CG70.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CG70.
CleanExiMM_LEPREL2.
ExpressionAtlasiQ8CG70. baseline and differential.
GenevestigatoriQ8CG70.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "LEPREL1, a novel ER and Golgi resident member of the Leprecan family."
    Jaernum S., Kjellman C., Darabi A., Nilsson I., Edvardsen K., Aaman P.
    Biochem. Biophys. Res. Commun. 317:342-351(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes."
    Vranka J.A., Sakai L.Y., Bachinger H.P.
    J. Biol. Chem. 279:23615-23621(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 139-732.
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
    Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
    Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 165-732.

Entry informationi

Entry nameiP3H3_MOUSE
AccessioniPrimary (citable) accession number: Q8CG70
Secondary accession number(s): O88836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: February 4, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.