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Protein

Prolyl 3-hydroxylase 3

Gene

P3h3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi580Iron1
Metal bindingi582Iron1
Metal bindingi652Iron1
Active sitei662By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 3Imported (EC:1.14.11.7)
Alternative name(s):
Leprecan-like protein 21 Publication
Protein BBy similarity
Gene namesi
Name:P3h3Imported
Synonyms:Leprel21 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315208. P3h3.

Subcellular locationi

  • Endoplasmic reticulum PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000024036120 – 732Prolyl 3-hydroxylase 3Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi458N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8CG70.
PaxDbiQ8CG70.
PeptideAtlasiQ8CG70.
PRIDEiQ8CG70.

PTM databases

PhosphoSitePlusiQ8CG70.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023191.
CleanExiMM_LEPREL2.
ExpressionAtlasiQ8CG70. baseline and differential.
GenevisibleiQ8CG70. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023958.

Structurei

3D structure databases

ProteinModelPortaliQ8CG70.
SMRiQ8CG70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati39 – 72TPR 1Add BLAST34
Repeati152 – 185TPR 2Add BLAST34
Repeati214 – 247TPR 3Add BLAST34
Repeati312 – 345TPR 4Add BLAST34
Domaini557 – 671Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST115

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili674 – 703Sequence analysisAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi729 – 732Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 39Pro-richAdd BLAST26
Compositional biasi689 – 697Poly-Glu9

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG4459. Eukaryota.
ENOG410XPXF. LUCA.
GeneTreeiENSGT00550000074573.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ8CG70.
OMAiRAYYQLK.
OrthoDBiEOG091G06WO.
PhylomeDBiQ8CG70.
TreeFamiTF320837.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLLRLLLL LLLPPPGSPE PPEPPGLAQL SPGSPPQAPD LLYADGLRAY
60 70 80 90 100
SAGAWAPAVA LLREALRSRA ALGRARQECG ASCAAEPGAA LPSQLLGAPH
110 120 130 140 150
PVSGPGVWEP LLLRATLRRA ECLTQCAVRR LGPGGAARLR VGSALRDAFR
160 170 180 190 200
RREPYNYLQR AYYQLKKLDL AASAAHTFFV ANPTHLQMRE DMAKYRRMSA
210 220 230 240 250
IRPQSFRDLV TPLYWAAYDT GLELLEQREA ALALPQLEEA LQGSLAHMES
260 270 280 290 300
CRAACEGPEE HQGAEEEGEG SQGGLYEAIA GHWIRVLQCR QHCVADTATR
310 320 330 340 350
PGRSFPVQDF LLSQLRRLHE AYAQVGNMSQ AMENVLSVLL FYPEDEAAKK
360 370 380 390 400
ALNQYQTQLG EPRPDLGPRE DIQRFILRSL GEKRQLYYAM EHLGTSFKDP
410 420 430 440 450
DSWTPEALIP KALRERLRED QEKKPWDHQP PQQKPLAHWK DALLMEGVTL
460 470 480 490 500
TQDAQQLNGS ERAVLDGLLT SAECGVLLQL AKDAAQAGAR SGYRGRRSPH
510 520 530 540 550
SPHERFEGLT VLKAAQLARA GTVGRPGAKL LLEVSERVRT LTQAYFSPER
560 570 580 590 600
PLHLSFTHLV CRSAIEGEQE QRMDLSHPVH ADNCVLDPDT GECWREPPAY
610 620 630 640 650
TYRDYSGLLY LNDDFKGGDL FFTQPNALTV TAQVRPRCGR LVAFSSGGEN
660 670 680 690 700
PHGVWAVTRG RRCALALWHT WAPEHSEQEW TEAKELLQEE EEEEEEEDIL
710 720 730
SRDPSPEPPS HKLQRVQEKA GKPRRVRVRE EL
Length:732
Mass (Da):81,701
Last modified:March 1, 2003 - v1
Checksum:iBD54733B60F1687A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ441086 mRNA. Translation: CAD29580.1.
AY463530 mRNA. Translation: AAS45239.1.
BC003726 mRNA. Translation: AAH03726.1.
BC016431 mRNA. Translation: AAH16431.1.
AC002397 Genomic DNA. Translation: AAC36012.1.
CCDSiCCDS20533.1.
RefSeqiNP_038562.2. NM_013534.4.
UniGeneiMm.35708.
Mm.9002.

Genome annotation databases

EnsembliENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191.
GeneIDi14789.
KEGGimmu:14789.
UCSCiuc009dsf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ441086 mRNA. Translation: CAD29580.1.
AY463530 mRNA. Translation: AAS45239.1.
BC003726 mRNA. Translation: AAH03726.1.
BC016431 mRNA. Translation: AAH16431.1.
AC002397 Genomic DNA. Translation: AAC36012.1.
CCDSiCCDS20533.1.
RefSeqiNP_038562.2. NM_013534.4.
UniGeneiMm.35708.
Mm.9002.

3D structure databases

ProteinModelPortaliQ8CG70.
SMRiQ8CG70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023958.

PTM databases

PhosphoSitePlusiQ8CG70.

Proteomic databases

MaxQBiQ8CG70.
PaxDbiQ8CG70.
PeptideAtlasiQ8CG70.
PRIDEiQ8CG70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191.
GeneIDi14789.
KEGGimmu:14789.
UCSCiuc009dsf.1. mouse.

Organism-specific databases

CTDi10536.
MGIiMGI:1315208. P3h3.

Phylogenomic databases

eggNOGiKOG4459. Eukaryota.
ENOG410XPXF. LUCA.
GeneTreeiENSGT00550000074573.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ8CG70.
OMAiRAYYQLK.
OrthoDBiEOG091G06WO.
PhylomeDBiQ8CG70.
TreeFamiTF320837.

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.

Miscellaneous databases

ChiTaRSiLeprel2. mouse.
PROiQ8CG70.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023191.
CleanExiMM_LEPREL2.
ExpressionAtlasiQ8CG70. baseline and differential.
GenevisibleiQ8CG70. MM.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP3H3_MOUSE
AccessioniPrimary (citable) accession number: Q8CG70
Secondary accession number(s): O88836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.