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Q8CG70

- P3H3_MOUSE

UniProt

Q8CG70 - P3H3_MOUSE

Protein

Prolyl 3-hydroxylase 3

Gene

Leprel2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.By similarity

    Catalytic activityi

    L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

    Cofactori

    Iron.By similarity
    Ascorbate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi580 – 5801Iron
    Metal bindingi582 – 5821Iron
    Metal bindingi652 – 6521Iron
    Active sitei662 – 6621By similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. L-ascorbic acid binding Source: UniProtKB-KW
    3. procollagen-proline 3-dioxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. negative regulation of cell proliferation Source: Ensembl

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding, Vitamin C

    Enzyme and pathway databases

    ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prolyl 3-hydroxylase 3 (EC:1.14.11.7)
    Alternative name(s):
    Leprecan-like protein 2
    Protein B
    Gene namesi
    Name:Leprel2
    Synonyms:P3h3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1315208. Leprel2.

    Subcellular locationi

    Endoplasmic reticulum PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 732713Prolyl 3-hydroxylase 3PRO_0000240361Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ8CG70.
    PRIDEiQ8CG70.

    PTM databases

    PhosphoSiteiQ8CG70.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CG70.
    BgeeiQ8CG70.
    CleanExiMM_LEPREL2.
    GenevestigatoriQ8CG70.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CG70.
    SMRiQ8CG70. Positions 40-67, 214-242.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati39 – 7234TPR 1Add
    BLAST
    Repeati152 – 18534TPR 2Add
    BLAST
    Repeati214 – 24734TPR 3Add
    BLAST
    Repeati312 – 34534TPR 4Add
    BLAST
    Domaini557 – 671115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili674 – 70330Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi729 – 7324Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi14 – 3926Pro-richAdd
    BLAST
    Compositional biasi689 – 6979Poly-Glu

    Sequence similaritiesi

    Belongs to the leprecan family.Curated
    Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
    Contains 4 TPR repeats.Curated

    Keywords - Domaini

    Coiled coil, Repeat, Signal, TPR repeat

    Phylogenomic databases

    eggNOGiNOG269251.
    GeneTreeiENSGT00550000074573.
    HOGENOMiHOG000231087.
    HOVERGENiHBG053224.
    InParanoidiQ8CG70.
    OMAiRAYYQLK.
    OrthoDBiEOG7BZVSS.
    PhylomeDBiQ8CG70.
    TreeFamiTF320837.

    Family and domain databases

    Gene3Di1.25.40.10. 3 hits.
    InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
    IPR006620. Pro_4_hyd_alph.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
    [Graphical view]
    SMARTiSM00702. P4Hc. 1 hit.
    [Graphical view]
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS51471. FE2OG_OXY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CG70-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRLLRLLLL LLLPPPGSPE PPEPPGLAQL SPGSPPQAPD LLYADGLRAY    50
    SAGAWAPAVA LLREALRSRA ALGRARQECG ASCAAEPGAA LPSQLLGAPH 100
    PVSGPGVWEP LLLRATLRRA ECLTQCAVRR LGPGGAARLR VGSALRDAFR 150
    RREPYNYLQR AYYQLKKLDL AASAAHTFFV ANPTHLQMRE DMAKYRRMSA 200
    IRPQSFRDLV TPLYWAAYDT GLELLEQREA ALALPQLEEA LQGSLAHMES 250
    CRAACEGPEE HQGAEEEGEG SQGGLYEAIA GHWIRVLQCR QHCVADTATR 300
    PGRSFPVQDF LLSQLRRLHE AYAQVGNMSQ AMENVLSVLL FYPEDEAAKK 350
    ALNQYQTQLG EPRPDLGPRE DIQRFILRSL GEKRQLYYAM EHLGTSFKDP 400
    DSWTPEALIP KALRERLRED QEKKPWDHQP PQQKPLAHWK DALLMEGVTL 450
    TQDAQQLNGS ERAVLDGLLT SAECGVLLQL AKDAAQAGAR SGYRGRRSPH 500
    SPHERFEGLT VLKAAQLARA GTVGRPGAKL LLEVSERVRT LTQAYFSPER 550
    PLHLSFTHLV CRSAIEGEQE QRMDLSHPVH ADNCVLDPDT GECWREPPAY 600
    TYRDYSGLLY LNDDFKGGDL FFTQPNALTV TAQVRPRCGR LVAFSSGGEN 650
    PHGVWAVTRG RRCALALWHT WAPEHSEQEW TEAKELLQEE EEEEEEEDIL 700
    SRDPSPEPPS HKLQRVQEKA GKPRRVRVRE EL 732
    Length:732
    Mass (Da):81,701
    Last modified:March 1, 2003 - v1
    Checksum:iBD54733B60F1687A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ441086 mRNA. Translation: CAD29580.1.
    AY463530 mRNA. Translation: AAS45239.1.
    BC003726 mRNA. Translation: AAH03726.1.
    BC016431 mRNA. Translation: AAH16431.1.
    AC002397 Genomic DNA. Translation: AAC36012.1.
    CCDSiCCDS20533.1.
    RefSeqiNP_038562.2. NM_013534.4.
    UniGeneiMm.35708.

    Genome annotation databases

    EnsembliENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191.
    GeneIDi14789.
    KEGGimmu:14789.
    UCSCiuc009dsf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ441086 mRNA. Translation: CAD29580.1 .
    AY463530 mRNA. Translation: AAS45239.1 .
    BC003726 mRNA. Translation: AAH03726.1 .
    BC016431 mRNA. Translation: AAH16431.1 .
    AC002397 Genomic DNA. Translation: AAC36012.1 .
    CCDSi CCDS20533.1.
    RefSeqi NP_038562.2. NM_013534.4.
    UniGenei Mm.35708.

    3D structure databases

    ProteinModelPortali Q8CG70.
    SMRi Q8CG70. Positions 40-67, 214-242.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8CG70.

    Proteomic databases

    PaxDbi Q8CG70.
    PRIDEi Q8CG70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023958 ; ENSMUSP00000023958 ; ENSMUSG00000023191 .
    GeneIDi 14789.
    KEGGi mmu:14789.
    UCSCi uc009dsf.1. mouse.

    Organism-specific databases

    CTDi 10536.
    MGIi MGI:1315208. Leprel2.

    Phylogenomic databases

    eggNOGi NOG269251.
    GeneTreei ENSGT00550000074573.
    HOGENOMi HOG000231087.
    HOVERGENi HBG053224.
    InParanoidi Q8CG70.
    OMAi RAYYQLK.
    OrthoDBi EOG7BZVSS.
    PhylomeDBi Q8CG70.
    TreeFami TF320837.

    Enzyme and pathway databases

    Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.

    Miscellaneous databases

    ChiTaRSi LEPREL2. mouse.
    NextBioi 286923.
    PROi Q8CG70.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CG70.
    Bgeei Q8CG70.
    CleanExi MM_LEPREL2.
    Genevestigatori Q8CG70.

    Family and domain databases

    Gene3Di 1.25.40.10. 3 hits.
    InterProi IPR005123. Oxoglu/Fe-dep_dioxygenase.
    IPR006620. Pro_4_hyd_alph.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF13640. 2OG-FeII_Oxy_3. 1 hit.
    [Graphical view ]
    SMARTi SM00702. P4Hc. 1 hit.
    [Graphical view ]
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS51471. FE2OG_OXY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LEPREL1, a novel ER and Golgi resident member of the Leprecan family."
      Jaernum S., Kjellman C., Darabi A., Nilsson I., Edvardsen K., Aaman P.
      Biochem. Biophys. Res. Commun. 317:342-351(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    2. "Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes."
      Vranka J.A., Sakai L.Y., Bachinger H.P.
      J. Biol. Chem. 279:23615-23621(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 139-732.
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
      Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
      Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 165-732.

    Entry informationi

    Entry nameiP3H3_MOUSE
    AccessioniPrimary (citable) accession number: Q8CG70
    Secondary accession number(s): O88836
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3