Reviewed, UniProtKB/Swiss-Prot Q8CG70 (P3H3_MOUSE)

Names and origin

Protein names

Recommended name:
    Prolyl 3-hydroxylase 3
    EC=1.14.11.7
Alternative name(s):
    Leprecan-like protein 2
    Protein B

Gene names

Name:	Leprel2
Synonyms:	P3h3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V By similarity.
Catalytic activity	Procollagen L-proline + 2-oxoglutarate + O(2) = procollagen trans-3-hydroxy-L-proline + succinate + CO(2).
Cofactor	Iron By similarity. Ascorbate By similarity.
Subcellular location	Endoplasmic reticulumBy similarity.
Sequence similarities	Belongs to the leprecan family. Contains 1 PKHD (prolyl/lysyl hydroxylase) domain. Contains 4 TPR repeats.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Coiled coil Repeat Signal TPR repeat
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: InterPro
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW procollagen-proline 3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 732	713	Prolyl 3-hydroxylase 3		PRO_0000240361
Regions
	Repeat	39 – 72	34	TPR 1
	Repeat	152 – 185	34	TPR 2
	Repeat	214 – 247	34	TPR 3
	Repeat	312 – 345	34	TPR 4
	Domain	460 – 670	211	PKHD
	Coiled coil	674 – 703	30	Potential
	Motif	729 – 732	4	Prevents secretion from ER Potential
	Compositional bias	14 – 39	26	Pro-rich
	Compositional bias	689 – 697	9	Poly-Glu
Sites
	Active site	662	1	By similarity
	Metal binding	580	1	Iron
	Metal binding	582	1	Iron
	Metal binding	652	1	Iron
Amino acid modifications
	Glycosylation	327	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8CG70-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BD54733B60F1687A	FASTA	732	81,701
	10 20 30 40 50 60 MLRLLRLLLL LLLPPPGSPE PPEPPGLAQL SPGSPPQAPD LLYADGLRAY SAGAWAPAVA 70 80 90 100 110 120 LLREALRSRA ALGRARQECG ASCAAEPGAA LPSQLLGAPH PVSGPGVWEP LLLRATLRRA 130 140 150 160 170 180 ECLTQCAVRR LGPGGAARLR VGSALRDAFR RREPYNYLQR AYYQLKKLDL AASAAHTFFV 190 200 210 220 230 240 ANPTHLQMRE DMAKYRRMSA IRPQSFRDLV TPLYWAAYDT GLELLEQREA ALALPQLEEA 250 260 270 280 290 300 LQGSLAHMES CRAACEGPEE HQGAEEEGEG SQGGLYEAIA GHWIRVLQCR QHCVADTATR 310 320 330 340 350 360 PGRSFPVQDF LLSQLRRLHE AYAQVGNMSQ AMENVLSVLL FYPEDEAAKK ALNQYQTQLG 370 380 390 400 410 420 EPRPDLGPRE DIQRFILRSL GEKRQLYYAM EHLGTSFKDP DSWTPEALIP KALRERLRED 430 440 450 460 470 480 QEKKPWDHQP PQQKPLAHWK DALLMEGVTL TQDAQQLNGS ERAVLDGLLT SAECGVLLQL 490 500 510 520 530 540 AKDAAQAGAR SGYRGRRSPH SPHERFEGLT VLKAAQLARA GTVGRPGAKL LLEVSERVRT 550 560 570 580 590 600 LTQAYFSPER PLHLSFTHLV CRSAIEGEQE QRMDLSHPVH ADNCVLDPDT GECWREPPAY 610 620 630 640 650 660 TYRDYSGLLY LNDDFKGGDL FFTQPNALTV TAQVRPRCGR LVAFSSGGEN PHGVWAVTRG 670 680 690 700 710 720 RRCALALWHT WAPEHSEQEW TEAKELLQEE EEEEEEEDIL SRDPSPEPPS HKLQRVQEKA 730 GKPRRVRVRE EL

References

[1]	"LEPREL1, a novel ER and Golgi resident member of the Leprecan family." Jaernum S., Kjellman C., Darabi A., Nilsson I., Edvardsen K., Aaman P. Biochem. Biophys. Res. Commun. 317:342-351(2004) [PubMed: 15063763] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes." Vranka J.A., Sakai L.Y., Bachinger H.P. J. Biol. Chem. 279:23615-23621(2004) [PubMed: 15044469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 139-732. Strain: FVB/N. Tissue: Mammary tumor.
[4]	"Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6." Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A. Genome Res. 8:29-40(1998) [PubMed: 9445485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 165-732.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ441086 mRNA. Translation: CAD29580.1. AY463530 mRNA. Translation: AAS45239.1. BC003726 mRNA. Translation: AAH03726.1. BC016431 mRNA. Translation: AAH16431.1. AC002397 Genomic DNA. Translation: AAC36012.1.
RefSeq	NP_038562.2.
UniGene	Mm.35708
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000023191. Mus musculus. [Contig view]
GeneID	14789.
KEGG	mmu:14789.
Organism-specific databases
MGI	MGI:1315208. Leprel2.
Phylogenomic databases
HOVERGEN	Q8CG70.
Gene expression databases
ArrayExpress	Q8CG70.
CleanEx	MM_LEPREL2.
GermOnline	ENSMUSG00000023191. Mus musculus.
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. IPR000886. ER_targeting_sequence. IPR006620. Pro_4_hyd_alph. IPR013026. TPR_region. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	286923.
SOURCE	Search...

Entry information

Entry name

P3H3_MOUSE

Accession

Primary (citable) accession number: Q8CG70
Secondary accession number(s): O88836

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 1, 2003
Last modified:	November 25, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)