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Protein

Structural maintenance of chromosomes protein 5

Gene

Smc5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi80 – 878ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Structural maintenance of chromosomes protein 5
Short name:
SMC protein 5
Short name:
SMC-5
Short name:
mSMC5
Alternative name(s):
Protein expressed in male leptotene and zygotene spermatocytes 453
Short name:
MLZ-453
Gene namesi
Name:Smc5
Synonyms:Kiaa0594, Smc5l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2385088. Smc5.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: MGI
  • chromosome, telomeric region Source: UniProtKB
  • interchromatin granule Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • PML body Source: UniProtKB
  • sex chromosome Source: UniProtKB
  • site of double-strand break Source: UniProtKB
  • Smc5-Smc6 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus, Telomere

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11011101Structural maintenance of chromosomes protein 5PRO_0000270952Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351PhosphoserineBy similarity

Post-translational modificationi

Sumoylated.By similarity
Ubiquitinated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CG46.
MaxQBiQ8CG46.
PaxDbiQ8CG46.
PRIDEiQ8CG46.

PTM databases

iPTMnetiQ8CG46.
PhosphoSiteiQ8CG46.

Expressioni

Tissue specificityi

Expressed in testis but not ovary.1 Publication

Developmental stagei

Expressed in ovary and testis at E15.5.1 Publication

Gene expression databases

BgeeiQ8CG46.
CleanExiMM_SMC5.
GenevisibleiQ8CG46. MM.

Interactioni

Subunit structurei

Forms a heterodimer with SMC6. Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NSMCE3. Interacts with NSMCE2. Interacts with SLF2; this interaction induces an association of the SLF1-SLF2 complex with the SMC5-SMC6 complex. Interacts with RAD18; this interaction is increased in a SLF1 or SLF2-dependent manner.By similarity

Protein-protein interaction databases

BioGridi230460. 1 interaction.
STRINGi10090.ENSMUSP00000084837.

Structurei

3D structure databases

ProteinModelPortaliQ8CG46.
SMRiQ8CG46. Positions 56-264, 919-1053.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni441 – 644204Flexible hingeAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili207 – 440234Sequence analysisAdd
BLAST
Coiled coili645 – 753109Sequence analysisAdd
BLAST
Coiled coili780 – 82849Sequence analysisAdd
BLAST
Coiled coili888 – 92740Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi991 – 102636Ala/Asp-rich (DA-box)Add
BLAST

Domaini

The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC6, forming a V-shaped heterodimer.By similarity

Sequence similaritiesi

Belongs to the SMC family. SMC5 subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0979. Eukaryota.
COG1196. LUCA.
GeneTreeiENSGT00550000074963.
HOGENOMiHOG000154342.
HOVERGENiHBG093963.
InParanoidiQ8CG46.
OMAiHTSQYFL.
OrthoDBiEOG72JWFH.
PhylomeDBiQ8CG46.
TreeFamiTF105708.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR027131. SMC5.
IPR033268. Smc5/Smc6.
[Graphical view]
PANTHERiPTHR19306. PTHR19306. 1 hit.
PTHR19306:SF1. PTHR19306:SF1. 1 hit.
PfamiPF02463. SMC_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CG46-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATPSGKAAP PNPQVSKRSL PRDASSEVPS KRKNSNPLPT LPRPSGTFVE
60 70 80 90 100
GSIVRIAMEN FLTYDICEVS PGPHLNMIIG ANGTGKSSIV CAICLGLAGK
110 120 130 140 150
PAFMGRADKV GFFVKRGCSK GLVEIELFRT SGNLIITREI DVIKNQSFWF
160 170 180 190 200
INKKPVTQKI VEEQVAALNI QVGNLCQFLP QDKVGEFAKL SKIELLEATE
210 220 230 240 250
KSVGPPEMHR YHCELKNFRE KEKQLETSCK EKTEYLEKMV QRNERYKQDV
260 270 280 290 300
ERFYERKRHL DLIEMLEAKR PWVEYENVRQ EYEGVKLIRD RVKEEVRKLK
310 320 330 340 350
EGQIPMTRRI EEIDRQRHTL EVRIKEKSTD IKEASQKCKQ RQDLIERKDR
360 370 380 390 400
QIKELQQALT VKQNEELDRQ KRISNTRKMI EDLQSELKTA ENCENLQPQI
410 420 430 440 450
DTVTNDLRRV QEEKALCEGE IIDKQREKEM LEKQRRSVSD HITRFDNLMN
460 470 480 490 500
QKEDKLRQRY RDTYDAVLWL RNNRDRFKQR VCEPIMLTIN MKDNKNAKYV
510 520 530 540 550
ENHISSNDLR AFVFESQEDM EIFLREVRDN KKLRVNAVIA PKISYADKAP
560 570 580 590 600
SRSLNDLKQY GFFSYLRELF DAPDPVMSYL CCQYHIHEVP VGTERTRERI
610 620 630 640 650
ERVIQETRLK QIYTAEEKYV LKTSVYSNKV ISSNTSLKVA QFLTVTVDLE
660 670 680 690 700
QRRHLEEQLK EMNRQLEAVD SGLAALRDTN RHLELKDNEL RLKKKELLER
710 720 730 740 750
KTRKRQLEQK ISSKLASIRL MEQDTCNLEE EERKASTKIK EINVQKAKLV
760 770 780 790 800
TELTGLVKIC TSFQIQKVDL ILQNTTVISE KNKLEADYMA SSSQLRVTEQ
810 820 830 840 850
QFIELDDNRQ RLLQKCKELM KKARQVCNLS ADQAVPQEFQ TQVPTIPNGH
860 870 880 890 900
SSSPPMAFQD LPNTLDEIDA LLTEERSRAS CFTGLNPSVV EEYSKREVEI
910 920 930 940 950
QQLTEELQGK KVELDEYREN ISQVKERWLN PLKELVEKIN EKFSNFFSSM
960 970 980 990 1000
QCAGEVDLHT ENEEDYDKYG IRIRVKFRSS TQLHELTPHH QSGGERSVST
1010 1020 1030 1040 1050
MLYLMALQEL NRCPFRVVDE INQGMDPINE RRVFEMVVNT ACKENTSQYF
1060 1070 1080 1090 1100
FITPKLLQNL PYSEKMTVLF VYNGPHMLEP NRWNLKAFQR RRRRITFTQP

Q
Length:1,101
Mass (Da):128,813
Last modified:March 1, 2003 - v1
Checksum:i71F7D790EE28C427
GO
Isoform 2 (identifier: Q8CG46-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     842-856: Missing.
     888-888: S → SV

Note: No experimental confirmation available.
Show »
Length:1,087
Mass (Da):127,382
Checksum:i0394ACA599DE1858
GO

Sequence cautioni

The sequence AAH07174.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti545 – 5451Y → C in AAH07174 (PubMed:12693553).Curated
Sequence conflicti675 – 6751A → T in AAH07174 (PubMed:12693553).Curated
Sequence conflicti842 – 8476QVPTIP → VSIKIS in BAC65603 (PubMed:12693553).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei842 – 85615Missing in isoform 2. 1 PublicationVSP_022251Add
BLAST
Alternative sequencei888 – 8881S → SV in isoform 2. 1 PublicationVSP_022252

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ534941 mRNA. Translation: CAD59184.1.
BC007174 mRNA. Translation: AAH07174.1. Different initiation.
BC038345 mRNA. Translation: AAH38345.1.
AK122321 Transcribed RNA. Translation: BAC65603.3.
CCDSiCCDS37936.1. [Q8CG46-2]
RefSeqiNP_001239613.1. NM_001252684.1. [Q8CG46-1]
NP_722503.1. NM_153808.2. [Q8CG46-2]
UniGeneiMm.23267.

Genome annotation databases

EnsembliENSMUST00000087556; ENSMUSP00000084837; ENSMUSG00000024943. [Q8CG46-2]
GeneIDi226026.
KEGGimmu:226026.
UCSCiuc008haa.2. mouse. [Q8CG46-2]
uc008hab.2. mouse. [Q8CG46-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ534941 mRNA. Translation: CAD59184.1.
BC007174 mRNA. Translation: AAH07174.1. Different initiation.
BC038345 mRNA. Translation: AAH38345.1.
AK122321 Transcribed RNA. Translation: BAC65603.3.
CCDSiCCDS37936.1. [Q8CG46-2]
RefSeqiNP_001239613.1. NM_001252684.1. [Q8CG46-1]
NP_722503.1. NM_153808.2. [Q8CG46-2]
UniGeneiMm.23267.

3D structure databases

ProteinModelPortaliQ8CG46.
SMRiQ8CG46. Positions 56-264, 919-1053.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230460. 1 interaction.
STRINGi10090.ENSMUSP00000084837.

PTM databases

iPTMnetiQ8CG46.
PhosphoSiteiQ8CG46.

Proteomic databases

EPDiQ8CG46.
MaxQBiQ8CG46.
PaxDbiQ8CG46.
PRIDEiQ8CG46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087556; ENSMUSP00000084837; ENSMUSG00000024943. [Q8CG46-2]
GeneIDi226026.
KEGGimmu:226026.
UCSCiuc008haa.2. mouse. [Q8CG46-2]
uc008hab.2. mouse. [Q8CG46-1]

Organism-specific databases

CTDi23137.
MGIiMGI:2385088. Smc5.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0979. Eukaryota.
COG1196. LUCA.
GeneTreeiENSGT00550000074963.
HOGENOMiHOG000154342.
HOVERGENiHBG093963.
InParanoidiQ8CG46.
OMAiHTSQYFL.
OrthoDBiEOG72JWFH.
PhylomeDBiQ8CG46.
TreeFamiTF105708.

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.

Miscellaneous databases

ChiTaRSiSmc5. mouse.
NextBioi377941.
PROiQ8CG46.
SOURCEiSearch...

Gene expression databases

BgeeiQ8CG46.
CleanExiMM_SMC5.
GenevisibleiQ8CG46. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
IPR027131. SMC5.
IPR033268. Smc5/Smc6.
[Graphical view]
PANTHERiPTHR19306. PTHR19306. 1 hit.
PTHR19306:SF1. PTHR19306:SF1. 1 hit.
PfamiPF02463. SMC_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The evolution of SMC proteins: phylogenetic analysis and structural implications."
    Cobbe N., Heck M.M.S.
    Mol. Biol. Evol. 21:332-347(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 326-1101 (ISOFORM 1).
    Tissue: Brain.
  4. "Characterization of a novel human SMC heterodimer homologous to the Schizosaccharomyces pombe Rad18/Spr18 complex."
    Taylor E.M., Moghraby J.S., Lees J.H., Smit B., Moens P.B., Lehmann A.R.
    Mol. Biol. Cell 12:1583-1594(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  6. "Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans."
    Kogo H., Kowa-Sugiyama H., Yamada K., Bolor H., Tsutsumi M., Ohye T., Inagaki H., Taniguchi M., Toda T., Kurahashi H.
    J. Hum. Genet. 55:293-299(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSMC5_MOUSE
AccessioniPrimary (citable) accession number: Q8CG46
Secondary accession number(s): Q80TW7, Q8CHX5, Q922K3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.