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Protein

Aflatoxin B1 aldehyde reductase member 2

Gene

Akr7a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. Acts as a 2-carboxybenzaldehyde reductase.1 Publication

Catalytic activityi

4-hydroxybutanoate + NADP+ = succinate semialdehyde + NADPH.

Kineticsi

Has a low KM for succinic semialdehyde.

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Binding sitei80 – 801NADPBy similarity
      Active sitei85 – 851Proton donorBy similarity
      Sitei113 – 1131Lowers pKa of active site TyrBy similarity
      Binding sitei149 – 1491SubstrateBy similarity
      Binding sitei205 – 2051NADPBy similarity
      Binding sitei258 – 2581NADPBy similarity
      Binding sitei268 – 2681SubstrateBy similarity
      Binding sitei271 – 2711SubstrateBy similarity
      Binding sitei367 – 3671SubstrateBy similarity

      Regions

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Nucleotide bindingi179 – 1802NADPBy similarity
      Nucleotide bindingi234 – 24411NADPBy similarityAdd
      BLAST
      Nucleotide bindingi326 – 3349NADPBy similarity

      GO - Molecular functioni

      • alditol:NADP+ 1-oxidoreductase activity Source: RGD
      • aldo-keto reductase (NADP) activity Source: RGD
      Complete GO annotation...

      Keywords - Molecular functioni

      Oxidoreductase

      Keywords - Ligandi

      NADP

      Enzyme and pathway databases

      SABIO-RKQ8CG45.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Aflatoxin B1 aldehyde reductase member 2 (EC:1.1.1.n11)
      Short name:
      rAFAR2
      Alternative name(s):
      Succinic semialdehyde reductase
      Short name:
      SSA reductase
      Gene namesi
      Name:Akr7a2
      Synonyms:Afar2, Aiar
      OrganismiRattus norvegicus (Rat)
      Taxonomic identifieri10116 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
      Proteomesi
      • UP000002494 Componenti: Unplaced

      Organism-specific databases

      RGDi620311. Akr7a2.

      Subcellular locationi

      • Golgi apparatusGolgi stack 1 Publication
      • Cytoplasm 1 Publication

      • Note: Its association with the Golgi stack may facilitate secretion of GHB.

      GO - Cellular componenti

      • Golgi apparatus Source: RGD
      • Golgi stack Source: UniProtKB-SubCell
      Complete GO annotation...

      Keywords - Cellular componenti

      Cytoplasm, Golgi apparatus

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 367367Aflatoxin B1 aldehyde reductase member 2PRO_0000070377Add
      BLAST

      Amino acid modifications

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Modified residuei10 – 101PhosphoserineBy similarity
      Modified residuei40 – 401PhosphoserineBy similarity
      Modified residuei48 – 481PhosphothreonineBy similarity
      Modified residuei121 – 1211PhosphoserineBy similarity
      Modified residuei136 – 1361N6-acetyllysineBy similarity
      Modified residuei244 – 2441N6-succinyllysineBy similarity
      Modified residuei263 – 2631PhosphoserineCombined sources

      Keywords - PTMi

      Acetylation, Phosphoprotein

      Proteomic databases

      PaxDbiQ8CG45.
      PRIDEiQ8CG45.

      PTM databases

      iPTMnetiQ8CG45.
      PhosphoSiteiQ8CG45.

      Interactioni

      Subunit structurei

      Homodimer. Heterodimer with AKR7A1.1 Publication

      Protein-protein interaction databases

      MINTiMINT-4567597.
      STRINGi10116.ENSRNOP00000024063.

      Structurei

      3D structure databases

      ProteinModelPortaliQ8CG45.
      SMRiQ8CG45. Positions 43-367.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Sequence similaritiesi

      Phylogenomic databases

      eggNOGiENOG410IDYD. Eukaryota.
      COG0667. LUCA.
      HOGENOMiHOG000250286.
      HOVERGENiHBG050576.
      InParanoidiQ8CG45.
      KOiK15303.
      PhylomeDBiQ8CG45.
      TreeFamiTF329173.

      Family and domain databases

      Gene3Di3.20.20.100. 1 hit.
      InterProiIPR001395. Aldo/ket_red/Kv-b.
      IPR023210. NADP_OxRdtase_dom.
      [Graphical view]
      PANTHERiPTHR11732. PTHR11732. 2 hits.
      PfamiPF00248. Aldo_ket_red. 1 hit.
      [Graphical view]
      SUPFAMiSSF51430. SSF51430. 1 hit.

      Sequencei

      Sequence statusi: Complete.

      Q8CG45-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20         30         40         50
      MLRAVSRAVS RAAVRCAWRS GPSVARPLAM SRSPAPRAVS GAPLRPGTVL
      60 70 80 90 100
      GTMEMGRRMD ASASAATVRA FLERGLNELD TAFMYCDGQS ESILGSLGLG
      110 120 130 140 150
      LGSGDCTVKI ATKANPWDGK SLKPDSVRSQ LETSLKRLQC PRVDLFYLHA
      160 170 180 190 200
      PDHGTPIVET LQACQQLHQE GKFVELGLSN YASWEVAEIY TLCKSNGWIL
      210 220 230 240 250
      PTVYQGMYNA TTRQVETELL PCLRYFGLRF YAYNPLAGGL LTGKYRYEDK
      260 270 280 290 300
      DGKQPEGRFF GNSWSETYRN RFWKEHHFEA IALVEKALKT TYGTSAPSMT
      310 320 330 340 350
      SAALRWMYHH SQLQGTRGDA VILGMSSLEQ LEQNLAATEE GPLEPAVVEA
      360
      FNQAWNVVAH ECPNYFR
      Length:367
      Mass (Da):40,675
      Last modified:December 15, 2003 - v2
      Checksum:iAA27A3FBB454FEC5
      GO

      Sequence cautioni

      The sequence AAH61816.1 differs from that shown. Reason: Erroneous initiation. Curated
      The sequence BAA90396.1 differs from that shown. Reason: Erroneous initiation. Curated
      The sequence CAC81080.1 differs from that shown. Reason: Erroneous initiation. Curated

      Experimental Info

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Sequence conflicti136 – 1361K → N in AAN03824 (PubMed:12071861).Curated
      Sequence conflicti295 – 2951S → D in AAN03824 (PubMed:12071861).Curated
      Sequence conflicti299 – 3002MT → ND in CAC81080 (PubMed:12879023).Curated

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AF503514 mRNA. Translation: AAN03824.1.
      BC061816 mRNA. Translation: AAH61816.1. Different initiation.
      AJ271883 mRNA. Translation: CAC81080.1. Different initiation.
      AB037424 mRNA. Translation: BAA90396.1. Different initiation.
      RefSeqiNP_599234.1. NM_134407.1.
      UniGeneiRn.8548.

      Genome annotation databases

      GeneIDi171445.
      KEGGirno:171445.
      UCSCiRGD:620311. rat.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AF503514 mRNA. Translation: AAN03824.1.
      BC061816 mRNA. Translation: AAH61816.1. Different initiation.
      AJ271883 mRNA. Translation: CAC81080.1. Different initiation.
      AB037424 mRNA. Translation: BAA90396.1. Different initiation.
      RefSeqiNP_599234.1. NM_134407.1.
      UniGeneiRn.8548.

      3D structure databases

      ProteinModelPortaliQ8CG45.
      SMRiQ8CG45. Positions 43-367.
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      MINTiMINT-4567597.
      STRINGi10116.ENSRNOP00000024063.

      PTM databases

      iPTMnetiQ8CG45.
      PhosphoSiteiQ8CG45.

      Proteomic databases

      PaxDbiQ8CG45.
      PRIDEiQ8CG45.

      Protocols and materials databases

      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      GeneIDi171445.
      KEGGirno:171445.
      UCSCiRGD:620311. rat.

      Organism-specific databases

      CTDi8574.
      RGDi620311. Akr7a2.

      Phylogenomic databases

      eggNOGiENOG410IDYD. Eukaryota.
      COG0667. LUCA.
      HOGENOMiHOG000250286.
      HOVERGENiHBG050576.
      InParanoidiQ8CG45.
      KOiK15303.
      PhylomeDBiQ8CG45.
      TreeFamiTF329173.

      Enzyme and pathway databases

      SABIO-RKQ8CG45.

      Miscellaneous databases

      NextBioi622372.
      PROiQ8CG45.

      Family and domain databases

      Gene3Di3.20.20.100. 1 hit.
      InterProiIPR001395. Aldo/ket_red/Kv-b.
      IPR023210. NADP_OxRdtase_dom.
      [Graphical view]
      PANTHERiPTHR11732. PTHR11732. 2 hits.
      PfamiPF00248. Aldo_ket_red. 1 hit.
      [Graphical view]
      SUPFAMiSSF51430. SSF51430. 1 hit.
      ProtoNetiSearch...

      Publicationsi

      « Hide 'large scale' publications
      1. "Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of aldo-keto reductase 7 family proteins."
        Kelly V.P., Sherratt P.J., Crouch D.H., Hayes J.D.
        Biochem. J. 366:847-861(2002) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 4-14 AND 33-44, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
        Strain: Fischer 344.
        Tissue: Liver.
      2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
        The MGC Project Team
        Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-367.
        Tissue: Prostate.
      3. "Aflatoxin B1 aldehyde reductase (AFAR) genes cluster at 1p35-1p36.1 in a region frequently altered in human tumour cells."
        Praml C., Savelyeva L., Schwab M.
        Oncogene 22:4765-4773(2003) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-367.
      4. "Androgen-regulated expression of a novel member of the aldo-keto reductase superfamily in regrowing rat prostate."
        Nishi N., Shoji H., Miyanaka H., Nakamura T.
        Endocrinology 141:3194-3199(2000) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-367.
        Strain: Sprague-Dawley.
      5. Lubec G., Afjehi-Sadat L.
        Submitted (NOV-2006) to UniProtKB
        Cited for: PROTEIN SEQUENCE OF 230-244; 259-269 AND 306-317, IDENTIFICATION BY MASS SPECTROMETRY.
        Strain: Sprague-Dawley.
        Tissue: Spinal cord.
      6. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
        Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
        Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
        Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

      Entry informationi

      Entry nameiARK72_RAT
      AccessioniPrimary (citable) accession number: Q8CG45
      Secondary accession number(s): Q6P765, Q8K435, Q9JM82
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: December 15, 2003
      Last sequence update: December 15, 2003
      Last modified: February 17, 2016
      This is version 107 of the entry and version 2 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program

      Miscellaneousi

      Miscellaneous

      With 4-nitrobenzaldehyde as substrate, it exhibits a substantially greater specic activity with NADPH than with NADH. Conversely, it has a 1.8-fold higher activity towards succinic semialdehyde with NADH than with NADPH.

      Keywords - Technical termi

      Complete proteome, Direct protein sequencing, Reference proteome

      Documents

      1. SIMILARITY comments
        Index of protein domains and families

      Similar proteinsi

      Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
      100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
      90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
      50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.