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Protein

Complement C1r-A subcomponent

Gene

C1ra

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Catalytic activityi

Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei501Charge relay systemBy similarity1
Active sitei559Charge relay systemBy similarity1
Active sitei656Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-166663. Initial triggering of complement.
R-MMU-173623. Classical antibody-mediated complement activation.

Protein family/group databases

MEROPSiS01.209.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C1r-A subcomponent (EC:3.4.21.41)
Alternative name(s):
Complement component 1 subcomponent r-A
Cleaved into the following 2 chains:
Gene namesi
Name:C1ra
Synonyms:C1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1355313. C1ra.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16By similarityAdd BLAST16
ChainiPRO_000002758017 – 707Complement C1r-A subcomponentAdd BLAST691
ChainiPRO_000002758117 – 462Complement C1r-A subcomponent heavy chainBy similarityAdd BLAST446
ChainiPRO_0000027582463 – 707Complement C1r-A subcomponent light chainBy similarityAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 88By similarity
Glycosylationi124N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi145 ↔ 164By similarity
Disulfide bondi160 ↔ 173By similarity
Modified residuei166(3R)-3-hydroxyasparagineBy similarity1
Disulfide bondi175 ↔ 188By similarity
Disulfide bondi192 ↔ 219By similarity
Modified residuei205Phosphoserine; by CK2By similarity1
Glycosylationi220N-linked (GlcNAc...)1 Publication1
Disulfide bondi249 ↔ 267By similarity
Disulfide bondi308 ↔ 357By similarity
Disulfide bondi337 ↔ 370By similarity
Disulfide bondi375 ↔ 428By similarity
Disulfide bondi405 ↔ 446By similarity
Disulfide bondi450 ↔ 579Interchain (between heavy and light chains)PROSITE-ProRule annotation
Glycosylationi583N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi622 ↔ 641By similarity
Disulfide bondi652 ↔ 682By similarity

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, Phosphoprotein

Proteomic databases

MaxQBiQ8CG16.
PaxDbiQ8CG16.
PeptideAtlasiQ8CG16.
PRIDEiQ8CG16.

PTM databases

PhosphoSitePlusiQ8CG16.

Expressioni

Gene expression databases

BgeeiENSMUSG00000055172.
CleanExiMM_C1R.
GenevisibleiQ8CG16. MM.

Interactioni

Subunit structurei

C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063707.

Structurei

3D structure databases

ProteinModelPortaliQ8CG16.
SMRiQ8CG16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 140CUB 1PROSITE-ProRule annotationAdd BLAST124
Domaini141 – 189EGF-like; calcium-bindingSequence analysisAdd BLAST49
Domaini192 – 304CUB 2PROSITE-ProRule annotationAdd BLAST113
Domaini306 – 372Sushi 1PROSITE-ProRule annotationAdd BLAST67
Domaini373 – 448Sushi 2PROSITE-ProRule annotationAdd BLAST76
Domaini463 – 704Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 2 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118890.
HOGENOMiHOG000237311.
HOVERGENiHBG000559.
InParanoidiQ8CG16.
KOiK01330.
OMAiQYYCHEP.
OrthoDBiEOG091G02DS.
PhylomeDBiQ8CG16.
TreeFamiTF330373.

Family and domain databases

CDDicd00033. CCP. 2 hits.
cd00041. CUB. 2 hits.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP_dom.
IPR001254. Trypsin_dom.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP
60 70 80 90 100
MGYRVKLVFW QFDVEPSEGC FYDYVKISAD KQTLGRFCGQ LDSPLGNPPG
110 120 130 140 150
SKEFMSQGNK MLLTFHTDFS NEENGTIMFY KGFLAYYQAV DLDECASQPN
160 170 180 190 200
SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE LQKDGQSCQA ECSSELYTEP
210 220 230 240 250
SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE IDDHQQVHCP
260 270 280 290 300
YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH
310 320 330 340 350
YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFIVTCKQG YQLMEGNQAL
360 370 380 390 400
LSFTAVCQND GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS
410 420 430 440 450
IQYHCHEPYY KMLTRAGSSE SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC
460 470 480 490 500
GKPVNPVTQK ERIIRGQPAR PGNFPWQAFT TTHGRGGGAL LGDRWILTAA
510 520 530 540 550
HTIYPKHHNK ENDNANPKML VFLGHTNVEQ IKKLGHHPVR RVIIHPDYRQ
560 570 580 590 600
DEPNNFEGDI ALLELENSVT LGPELLPICL PDNETFYGQG LMGYVSGFGI
610 620 630 640 650
TEDKLAFDLR FVRLPVADSE ACQRWLQTKK DTSPFSQNMF CSGDPAVQQD
660 670 680 690 700
ACQGDSGGVF AVRDRNRDIW VATGIVSWGI GCGEGYGFYT KVLNYVDWIK

KEMGDEN
Length:707
Mass (Da):80,073
Last modified:March 1, 2003 - v1
Checksum:i29DAAEB3C047D8E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101S → R in AAG01898 (Ref. 1) Curated1
Sequence conflicti359N → H in AAH04637 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148216 mRNA. Translation: AAG01898.1.
AF459011
, AF459008, AF459009, AF459010 Genomic DNA. Translation: AAO15553.1.
BC004637 mRNA. Translation: AAH04637.1.
CCDSiCCDS20521.1.
RefSeqiNP_075632.3. NM_023143.3.
UniGeneiMm.333375.

Genome annotation databases

EnsembliENSMUST00000068593; ENSMUSP00000063707; ENSMUSG00000055172.
GeneIDi50909.
KEGGimmu:50909.
UCSCiuc009dra.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148216 mRNA. Translation: AAG01898.1.
AF459011
, AF459008, AF459009, AF459010 Genomic DNA. Translation: AAO15553.1.
BC004637 mRNA. Translation: AAH04637.1.
CCDSiCCDS20521.1.
RefSeqiNP_075632.3. NM_023143.3.
UniGeneiMm.333375.

3D structure databases

ProteinModelPortaliQ8CG16.
SMRiQ8CG16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063707.

Protein family/group databases

MEROPSiS01.209.

PTM databases

PhosphoSitePlusiQ8CG16.

Proteomic databases

MaxQBiQ8CG16.
PaxDbiQ8CG16.
PeptideAtlasiQ8CG16.
PRIDEiQ8CG16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068593; ENSMUSP00000063707; ENSMUSG00000055172.
GeneIDi50909.
KEGGimmu:50909.
UCSCiuc009dra.2. mouse.

Organism-specific databases

CTDi50909.
MGIiMGI:1355313. C1ra.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118890.
HOGENOMiHOG000237311.
HOVERGENiHBG000559.
InParanoidiQ8CG16.
KOiK01330.
OMAiQYYCHEP.
OrthoDBiEOG091G02DS.
PhylomeDBiQ8CG16.
TreeFamiTF330373.

Enzyme and pathway databases

ReactomeiR-MMU-166663. Initial triggering of complement.
R-MMU-173623. Classical antibody-mediated complement activation.

Miscellaneous databases

PROiQ8CG16.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055172.
CleanExiMM_C1R.
GenevisibleiQ8CG16. MM.

Family and domain databases

CDDicd00033. CCP. 2 hits.
cd00041. CUB. 2 hits.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP_dom.
IPR001254. Trypsin_dom.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00181. EGF. 1 hit.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC1RA_MOUSE
AccessioniPrimary (citable) accession number: Q8CG16
Secondary accession number(s): Q99KI6, Q9ET60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.