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Q8CG16 (C1RA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Complement C1r-A subcomponent

EC=3.4.21.41
Alternative name(s):
Complement component 1 subcomponent r-A
Gene names
Name:C1ra
Synonyms:C1r
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length707 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Catalytic activity

Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).

Subunit structure

C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds By similarity.

Post-translational modification

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 2 CUB domains.

Contains 1 EGF-like domain.

Contains 1 peptidase S1 domain.

Contains 2 Sushi (CCP/SCR) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 By similarity
Chain17 – 707691Complement C1r-A subcomponent
PRO_0000027580
Chain17 – 462446Complement C1r-A subcomponent heavy chain By similarity
PRO_0000027581
Chain463 – 707245Complement C1r-A subcomponent light chain By similarity
PRO_0000027582

Regions

Domain17 – 140124CUB 1
Domain141 – 18949EGF-like; calcium-binding Potential
Domain192 – 304113CUB 2
Domain306 – 37267Sushi 1
Domain373 – 44876Sushi 2
Domain463 – 704242Peptidase S1

Sites

Active site5011Charge relay system By similarity
Active site5591Charge relay system By similarity
Active site6561Charge relay system By similarity

Amino acid modifications

Modified residue1661(3R)-3-hydroxyasparagine By similarity
Modified residue2051Phosphoserine; by CK2 By similarity
Glycosylation1241N-linked (GlcNAc...) Potential
Glycosylation2201N-linked (GlcNAc...) Ref.4
Glycosylation5831N-linked (GlcNAc...) Potential
Disulfide bond70 ↔ 88 By similarity
Disulfide bond145 ↔ 164 By similarity
Disulfide bond160 ↔ 173 By similarity
Disulfide bond175 ↔ 188 By similarity
Disulfide bond192 ↔ 219 By similarity
Disulfide bond249 ↔ 267 By similarity
Disulfide bond308 ↔ 357 By similarity
Disulfide bond337 ↔ 370 By similarity
Disulfide bond375 ↔ 428 By similarity
Disulfide bond405 ↔ 446 By similarity
Disulfide bond450 ↔ 579Interchain (between heavy and light chains) By similarity
Disulfide bond622 ↔ 641 By similarity
Disulfide bond652 ↔ 682 By similarity

Experimental info

Sequence conflict1011S → R in AAG01898. Ref.1
Sequence conflict3591N → H in AAH04637. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8CG16 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 29DAAEB3C047D8E8

FASTA70780,073
        10         20         30         40         50         60 
MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP MGYRVKLVFW 

        70         80         90        100        110        120 
QFDVEPSEGC FYDYVKISAD KQTLGRFCGQ LDSPLGNPPG SKEFMSQGNK MLLTFHTDFS 

       130        140        150        160        170        180 
NEENGTIMFY KGFLAYYQAV DLDECASQPN SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE 

       190        200        210        220        230        240 
LQKDGQSCQA ECSSELYTEP SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE 

       250        260        270        280        290        300 
IDDHQQVHCP YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH 

       310        320        330        340        350        360 
YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFIVTCKQG YQLMEGNQAL LSFTAVCQND 

       370        380        390        400        410        420 
GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS IQYHCHEPYY KMLTRAGSSE 

       430        440        450        460        470        480 
SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC GKPVNPVTQK ERIIRGQPAR PGNFPWQAFT 

       490        500        510        520        530        540 
TTHGRGGGAL LGDRWILTAA HTIYPKHHNK ENDNANPKML VFLGHTNVEQ IKKLGHHPVR 

       550        560        570        580        590        600 
RVIIHPDYRQ DEPNNFEGDI ALLELENSVT LGPELLPICL PDNETFYGQG LMGYVSGFGI 

       610        620        630        640        650        660 
TEDKLAFDLR FVRLPVADSE ACQRWLQTKK DTSPFSQNMF CSGDPAVQQD ACQGDSGGVF 

       670        680        690        700 
AVRDRNRDIW VATGIVSWGI GCGEGYGFYT KVLNYVDWIK KEMGDEN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a cDNA encoding a serine protease homologous to human complement C1r precursor from an allografted mouse skin and its expression in Escherichia coli."
Byun S.J., Hyun J.H., Hwang H.Y., Ryoo Z.Y., Kim T.Y.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system."
Garnier G., Circolo A., Xu Y., Volanakis J.E.
Biochem. J. 371:631-640(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: 129/SvJ.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
Bernhard O.K., Kapp E.A., Simpson R.J.
J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-220.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF148216 mRNA. Translation: AAG01898.1.
AF459011 expand/collapse EMBL AC list , AF459008, AF459009, AF459010 Genomic DNA. Translation: AAO15553.1.
BC004637 mRNA. Translation: AAH04637.1.
CCDSCCDS20521.1.
RefSeqNP_075632.3. NM_023143.3.
UniGeneMm.333375.

3D structure databases

ProteinModelPortalQ8CG16.
SMRQ8CG16. Positions 24-703.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000063707.

Protein family/group databases

MEROPSS01.192.

PTM databases

PhosphoSiteQ8CG16.

Proteomic databases

PaxDbQ8CG16.
PRIDEQ8CG16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000068593; ENSMUSP00000063707; ENSMUSG00000055172.
GeneID50909.
KEGGmmu:50909.
UCSCuc009dra.2. mouse.

Organism-specific databases

CTD50909.
MGIMGI:1355313. C1ra.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00560000076882.
HOGENOMHOG000237311.
HOVERGENHBG000559.
InParanoidQ8CG16.
KOK01330.
OMAPRCLPVC.
OrthoDBEOG7W6WK4.
PhylomeDBQ8CG16.
TreeFamTF330373.

Gene expression databases

ArrayExpressQ8CG16.
BgeeQ8CG16.
CleanExMM_C1R.
GenevestigatorQ8CG16.

Family and domain databases

Gene3D2.60.120.290. 2 hits.
InterProIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio307891.
PROQ8CG16.
SOURCESearch...

Entry information

Entry nameC1RA_MOUSE
AccessionPrimary (citable) accession number: Q8CG16
Secondary accession number(s): Q99KI6, Q9ET60
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot