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Q8CG16

- C1RA_MOUSE

UniProt

Q8CG16 - C1RA_MOUSE

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Protein

Complement C1r-A subcomponent

Gene
C1ra, C1r
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Catalytic activityi

Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei501 – 5011Charge relay system By similarity
Active sitei559 – 5591Charge relay system By similarity
Active sitei656 – 6561Charge relay system By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. complement activation, classical pathway Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Protein family/group databases

MEROPSiS01.192.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C1r-A subcomponent (EC:3.4.21.41)
Alternative name(s):
Complement component 1 subcomponent r-A
Cleaved into the following 2 chains:
Gene namesi
Name:C1ra
Synonyms:C1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1355313. C1ra.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 By similarityAdd
BLAST
Chaini17 – 707691Complement C1r-A subcomponentPRO_0000027580Add
BLAST
Chaini17 – 462446Complement C1r-A subcomponent heavy chain By similarityPRO_0000027581Add
BLAST
Chaini463 – 707245Complement C1r-A subcomponent light chain By similarityPRO_0000027582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 88 By similarity
Glycosylationi124 – 1241N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi145 ↔ 164 By similarity
Disulfide bondi160 ↔ 173 By similarity
Modified residuei166 – 1661(3R)-3-hydroxyasparagine By similarity
Disulfide bondi175 ↔ 188 By similarity
Disulfide bondi192 ↔ 219 By similarity
Modified residuei205 – 2051Phosphoserine; by CK2 By similarity
Glycosylationi220 – 2201N-linked (GlcNAc...)1 Publication
Disulfide bondi249 ↔ 267 By similarity
Disulfide bondi308 ↔ 357 By similarity
Disulfide bondi337 ↔ 370 By similarity
Disulfide bondi375 ↔ 428 By similarity
Disulfide bondi405 ↔ 446 By similarity
Disulfide bondi450 ↔ 579Interchain (between heavy and light chains) By similarity
Glycosylationi583 – 5831N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi622 ↔ 641 By similarity
Disulfide bondi652 ↔ 682 By similarity

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CG16.
PRIDEiQ8CG16.

PTM databases

PhosphoSiteiQ8CG16.

Expressioni

Gene expression databases

ArrayExpressiQ8CG16.
BgeeiQ8CG16.
CleanExiMM_C1R.
GenevestigatoriQ8CG16.

Interactioni

Subunit structurei

C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063707.

Structurei

3D structure databases

ProteinModelPortaliQ8CG16.
SMRiQ8CG16. Positions 24-703.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 140124CUB 1Add
BLAST
Domaini141 – 18949EGF-like; calcium-binding Reviewed predictionAdd
BLAST
Domaini192 – 304113CUB 2Add
BLAST
Domaini306 – 37267Sushi 1Add
BLAST
Domaini373 – 44876Sushi 2Add
BLAST
Domaini463 – 704242Peptidase S1Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00560000076882.
HOGENOMiHOG000237311.
HOVERGENiHBG000559.
InParanoidiQ8CG16.
KOiK01330.
OMAiPRCLPVC.
OrthoDBiEOG7W6WK4.
PhylomeDBiQ8CG16.
TreeFamiTF330373.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG16-1 [UniParc]FASTAAdd to Basket

« Hide

MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP    50
MGYRVKLVFW QFDVEPSEGC FYDYVKISAD KQTLGRFCGQ LDSPLGNPPG 100
SKEFMSQGNK MLLTFHTDFS NEENGTIMFY KGFLAYYQAV DLDECASQPN 150
SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE LQKDGQSCQA ECSSELYTEP 200
SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE IDDHQQVHCP 250
YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH 300
YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFIVTCKQG YQLMEGNQAL 350
LSFTAVCQND GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS 400
IQYHCHEPYY KMLTRAGSSE SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC 450
GKPVNPVTQK ERIIRGQPAR PGNFPWQAFT TTHGRGGGAL LGDRWILTAA 500
HTIYPKHHNK ENDNANPKML VFLGHTNVEQ IKKLGHHPVR RVIIHPDYRQ 550
DEPNNFEGDI ALLELENSVT LGPELLPICL PDNETFYGQG LMGYVSGFGI 600
TEDKLAFDLR FVRLPVADSE ACQRWLQTKK DTSPFSQNMF CSGDPAVQQD 650
ACQGDSGGVF AVRDRNRDIW VATGIVSWGI GCGEGYGFYT KVLNYVDWIK 700
KEMGDEN 707
Length:707
Mass (Da):80,073
Last modified:March 1, 2003 - v1
Checksum:i29DAAEB3C047D8E8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011S → R in AAG01898. 1 Publication
Sequence conflicti359 – 3591N → H in AAH04637. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148216 mRNA. Translation: AAG01898.1.
AF459011
, AF459008, AF459009, AF459010 Genomic DNA. Translation: AAO15553.1.
BC004637 mRNA. Translation: AAH04637.1.
CCDSiCCDS20521.1.
RefSeqiNP_075632.3. NM_023143.3.
UniGeneiMm.333375.

Genome annotation databases

EnsembliENSMUST00000068593; ENSMUSP00000063707; ENSMUSG00000055172.
GeneIDi50909.
KEGGimmu:50909.
UCSCiuc009dra.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148216 mRNA. Translation: AAG01898.1 .
AF459011
, AF459008 , AF459009 , AF459010 Genomic DNA. Translation: AAO15553.1 .
BC004637 mRNA. Translation: AAH04637.1 .
CCDSi CCDS20521.1.
RefSeqi NP_075632.3. NM_023143.3.
UniGenei Mm.333375.

3D structure databases

ProteinModelPortali Q8CG16.
SMRi Q8CG16. Positions 24-703.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000063707.

Protein family/group databases

MEROPSi S01.192.

PTM databases

PhosphoSitei Q8CG16.

Proteomic databases

PaxDbi Q8CG16.
PRIDEi Q8CG16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068593 ; ENSMUSP00000063707 ; ENSMUSG00000055172 .
GeneIDi 50909.
KEGGi mmu:50909.
UCSCi uc009dra.2. mouse.

Organism-specific databases

CTDi 50909.
MGIi MGI:1355313. C1ra.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00560000076882.
HOGENOMi HOG000237311.
HOVERGENi HBG000559.
InParanoidi Q8CG16.
KOi K01330.
OMAi PRCLPVC.
OrthoDBi EOG7W6WK4.
PhylomeDBi Q8CG16.
TreeFami TF330373.

Miscellaneous databases

NextBioi 307891.
PROi Q8CG16.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8CG16.
Bgeei Q8CG16.
CleanExi MM_C1R.
Genevestigatori Q8CG16.

Family and domain databases

Gene3Di 2.60.120.290. 2 hits.
InterProi IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a cDNA encoding a serine protease homologous to human complement C1r precursor from an allografted mouse skin and its expression in Escherichia coli."
    Byun S.J., Hyun J.H., Hwang H.Y., Ryoo Z.Y., Kim T.Y.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system."
    Garnier G., Circolo A., Xu Y., Volanakis J.E.
    Biochem. J. 371:631-640(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-220.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiC1RA_MOUSE
AccessioniPrimary (citable) accession number: Q8CG16
Secondary accession number(s): Q99KI6, Q9ET60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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