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Q8CG16

- C1RA_MOUSE

UniProt

Q8CG16 - C1RA_MOUSE

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Protein

Complement C1r-A subcomponent

Gene

C1ra

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Catalytic activityi

Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei501 – 5011Charge relay systemBy similarity
Active sitei559 – 5591Charge relay systemBy similarity
Active sitei656 – 6561Charge relay systemBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. complement activation, classical pathway Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Protein family/group databases

MEROPSiS01.209.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C1r-A subcomponent (EC:3.4.21.41)
Alternative name(s):
Complement component 1 subcomponent r-A
Cleaved into the following 2 chains:
Gene namesi
Name:C1ra
Synonyms:C1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1355313. C1ra.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616By similarityAdd
BLAST
Chaini17 – 707691Complement C1r-A subcomponentPRO_0000027580Add
BLAST
Chaini17 – 462446Complement C1r-A subcomponent heavy chainBy similarityPRO_0000027581Add
BLAST
Chaini463 – 707245Complement C1r-A subcomponent light chainBy similarityPRO_0000027582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 88By similarity
Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi145 ↔ 164By similarity
Disulfide bondi160 ↔ 173By similarity
Modified residuei166 – 1661(3R)-3-hydroxyasparagineBy similarity
Disulfide bondi175 ↔ 188By similarity
Disulfide bondi192 ↔ 219By similarity
Modified residuei205 – 2051Phosphoserine; by CK2By similarity
Glycosylationi220 – 2201N-linked (GlcNAc...)1 Publication
Disulfide bondi249 ↔ 267By similarity
Disulfide bondi308 ↔ 357By similarity
Disulfide bondi337 ↔ 370By similarity
Disulfide bondi375 ↔ 428By similarity
Disulfide bondi405 ↔ 446By similarity
Disulfide bondi450 ↔ 579Interchain (between heavy and light chains)PROSITE-ProRule annotation
Glycosylationi583 – 5831N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi622 ↔ 641By similarity
Disulfide bondi652 ↔ 682By similarity

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, Phosphoprotein

Proteomic databases

PaxDbiQ8CG16.
PRIDEiQ8CG16.

PTM databases

PhosphoSiteiQ8CG16.

Expressioni

Gene expression databases

BgeeiQ8CG16.
CleanExiMM_C1R.
ExpressionAtlasiQ8CG16. baseline and differential.
GenevestigatoriQ8CG16.

Interactioni

Subunit structurei

C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063707.

Structurei

3D structure databases

ProteinModelPortaliQ8CG16.
SMRiQ8CG16. Positions 24-703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 140124CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini141 – 18949EGF-like; calcium-bindingSequence AnalysisAdd
BLAST
Domaini192 – 304113CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini306 – 37267Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini373 – 44876Sushi 2PROSITE-ProRule annotationAdd
BLAST
Domaini463 – 704242Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 1 EGF-like domain.Curated
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 2 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118890.
HOGENOMiHOG000237311.
HOVERGENiHBG000559.
InParanoidiQ8CG16.
KOiK01330.
OMAiPRCLPVC.
OrthoDBiEOG7W6WK4.
PhylomeDBiQ8CG16.
TreeFamiTF330373.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG16-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP
60 70 80 90 100
MGYRVKLVFW QFDVEPSEGC FYDYVKISAD KQTLGRFCGQ LDSPLGNPPG
110 120 130 140 150
SKEFMSQGNK MLLTFHTDFS NEENGTIMFY KGFLAYYQAV DLDECASQPN
160 170 180 190 200
SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE LQKDGQSCQA ECSSELYTEP
210 220 230 240 250
SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE IDDHQQVHCP
260 270 280 290 300
YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH
310 320 330 340 350
YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFIVTCKQG YQLMEGNQAL
360 370 380 390 400
LSFTAVCQND GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS
410 420 430 440 450
IQYHCHEPYY KMLTRAGSSE SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC
460 470 480 490 500
GKPVNPVTQK ERIIRGQPAR PGNFPWQAFT TTHGRGGGAL LGDRWILTAA
510 520 530 540 550
HTIYPKHHNK ENDNANPKML VFLGHTNVEQ IKKLGHHPVR RVIIHPDYRQ
560 570 580 590 600
DEPNNFEGDI ALLELENSVT LGPELLPICL PDNETFYGQG LMGYVSGFGI
610 620 630 640 650
TEDKLAFDLR FVRLPVADSE ACQRWLQTKK DTSPFSQNMF CSGDPAVQQD
660 670 680 690 700
ACQGDSGGVF AVRDRNRDIW VATGIVSWGI GCGEGYGFYT KVLNYVDWIK

KEMGDEN
Length:707
Mass (Da):80,073
Last modified:March 1, 2003 - v1
Checksum:i29DAAEB3C047D8E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011S → R in AAG01898. 1 PublicationCurated
Sequence conflicti359 – 3591N → H in AAH04637. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148216 mRNA. Translation: AAG01898.1.
AF459011
, AF459008, AF459009, AF459010 Genomic DNA. Translation: AAO15553.1.
BC004637 mRNA. Translation: AAH04637.1.
CCDSiCCDS20521.1.
RefSeqiNP_075632.3. NM_023143.3.
UniGeneiMm.333375.

Genome annotation databases

EnsembliENSMUST00000068593; ENSMUSP00000063707; ENSMUSG00000055172.
GeneIDi50909.
KEGGimmu:50909.
UCSCiuc009dra.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148216 mRNA. Translation: AAG01898.1 .
AF459011
, AF459008 , AF459009 , AF459010 Genomic DNA. Translation: AAO15553.1 .
BC004637 mRNA. Translation: AAH04637.1 .
CCDSi CCDS20521.1.
RefSeqi NP_075632.3. NM_023143.3.
UniGenei Mm.333375.

3D structure databases

ProteinModelPortali Q8CG16.
SMRi Q8CG16. Positions 24-703.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000063707.

Protein family/group databases

MEROPSi S01.209.

PTM databases

PhosphoSitei Q8CG16.

Proteomic databases

PaxDbi Q8CG16.
PRIDEi Q8CG16.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068593 ; ENSMUSP00000063707 ; ENSMUSG00000055172 .
GeneIDi 50909.
KEGGi mmu:50909.
UCSCi uc009dra.2. mouse.

Organism-specific databases

CTDi 50909.
MGIi MGI:1355313. C1ra.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00760000118890.
HOGENOMi HOG000237311.
HOVERGENi HBG000559.
InParanoidi Q8CG16.
KOi K01330.
OMAi PRCLPVC.
OrthoDBi EOG7W6WK4.
PhylomeDBi Q8CG16.
TreeFami TF330373.

Miscellaneous databases

NextBioi 307891.
PROi Q8CG16.
SOURCEi Search...

Gene expression databases

Bgeei Q8CG16.
CleanExi MM_C1R.
ExpressionAtlasi Q8CG16. baseline and differential.
Genevestigatori Q8CG16.

Family and domain databases

Gene3Di 2.60.120.290. 2 hits.
InterProi IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a cDNA encoding a serine protease homologous to human complement C1r precursor from an allografted mouse skin and its expression in Escherichia coli."
    Byun S.J., Hyun J.H., Hwang H.Y., Ryoo Z.Y., Kim T.Y.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system."
    Garnier G., Circolo A., Xu Y., Volanakis J.E.
    Biochem. J. 371:631-640(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-220.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiC1RA_MOUSE
AccessioniPrimary (citable) accession number: Q8CG16
Secondary accession number(s): Q99KI6, Q9ET60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3