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Q8CG16

- C1RA_MOUSE

UniProt

Q8CG16 - C1RA_MOUSE

Protein

Complement C1r-A subcomponent

Gene

C1ra

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

    Catalytic activityi

    Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei501 – 5011Charge relay systemBy similarity
    Active sitei559 – 5591Charge relay systemBy similarity
    Active sitei656 – 6561Charge relay systemBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. complement activation, classical pathway Source: UniProtKB-KW
    2. innate immune response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Complement pathway, Immunity, Innate immunity

    Protein family/group databases

    MEROPSiS01.192.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Complement C1r-A subcomponent (EC:3.4.21.41)
    Alternative name(s):
    Complement component 1 subcomponent r-A
    Cleaved into the following 2 chains:
    Gene namesi
    Name:C1ra
    Synonyms:C1r
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1355313. C1ra.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616By similarityAdd
    BLAST
    Chaini17 – 707691Complement C1r-A subcomponentPRO_0000027580Add
    BLAST
    Chaini17 – 462446Complement C1r-A subcomponent heavy chainBy similarityPRO_0000027581Add
    BLAST
    Chaini463 – 707245Complement C1r-A subcomponent light chainBy similarityPRO_0000027582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi70 ↔ 88By similarity
    Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi145 ↔ 164By similarity
    Disulfide bondi160 ↔ 173By similarity
    Modified residuei166 – 1661(3R)-3-hydroxyasparagineBy similarity
    Disulfide bondi175 ↔ 188By similarity
    Disulfide bondi192 ↔ 219By similarity
    Modified residuei205 – 2051Phosphoserine; by CK2By similarity
    Glycosylationi220 – 2201N-linked (GlcNAc...)1 Publication
    Disulfide bondi249 ↔ 267By similarity
    Disulfide bondi308 ↔ 357By similarity
    Disulfide bondi337 ↔ 370By similarity
    Disulfide bondi375 ↔ 428By similarity
    Disulfide bondi405 ↔ 446By similarity
    Disulfide bondi450 ↔ 579Interchain (between heavy and light chains)PROSITE-ProRule annotation
    Glycosylationi583 – 5831N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi622 ↔ 641By similarity
    Disulfide bondi652 ↔ 682By similarity

    Post-translational modificationi

    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Hydroxylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ8CG16.
    PRIDEiQ8CG16.

    PTM databases

    PhosphoSiteiQ8CG16.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8CG16.
    BgeeiQ8CG16.
    CleanExiMM_C1R.
    GenevestigatoriQ8CG16.

    Interactioni

    Subunit structurei

    C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000063707.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CG16.
    SMRiQ8CG16. Positions 24-703.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 140124CUB 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini141 – 18949EGF-like; calcium-bindingSequence AnalysisAdd
    BLAST
    Domaini192 – 304113CUB 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini306 – 37267Sushi 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini373 – 44876Sushi 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini463 – 704242Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 2 CUB domains.PROSITE-ProRule annotation
    Contains 1 EGF-like domain.Curated
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 2 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal, Sushi

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00560000076882.
    HOGENOMiHOG000237311.
    HOVERGENiHBG000559.
    InParanoidiQ8CG16.
    KOiK01330.
    OMAiPRCLPVC.
    OrthoDBiEOG7W6WK4.
    PhylomeDBiQ8CG16.
    TreeFamiTF330373.

    Family and domain databases

    Gene3Di2.60.120.290. 2 hits.
    InterProiIPR000859. CUB_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000436. Sushi_SCR_CCP.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00431. CUB. 2 hits.
    PF00084. Sushi. 2 hits.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00032. CCP. 2 hits.
    SM00042. CUB. 2 hits.
    SM00179. EGF_CA. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49854. SSF49854. 2 hits.
    SSF50494. SSF50494. 1 hit.
    SSF57535. SSF57535. 2 hits.
    PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
    PS01180. CUB. 2 hits.
    PS01186. EGF_2. 1 hit.
    PS01187. EGF_CA. 1 hit.
    PS50923. SUSHI. 2 hits.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8CG16-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP    50
    MGYRVKLVFW QFDVEPSEGC FYDYVKISAD KQTLGRFCGQ LDSPLGNPPG 100
    SKEFMSQGNK MLLTFHTDFS NEENGTIMFY KGFLAYYQAV DLDECASQPN 150
    SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE LQKDGQSCQA ECSSELYTEP 200
    SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE IDDHQQVHCP 250
    YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH 300
    YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFIVTCKQG YQLMEGNQAL 350
    LSFTAVCQND GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS 400
    IQYHCHEPYY KMLTRAGSSE SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC 450
    GKPVNPVTQK ERIIRGQPAR PGNFPWQAFT TTHGRGGGAL LGDRWILTAA 500
    HTIYPKHHNK ENDNANPKML VFLGHTNVEQ IKKLGHHPVR RVIIHPDYRQ 550
    DEPNNFEGDI ALLELENSVT LGPELLPICL PDNETFYGQG LMGYVSGFGI 600
    TEDKLAFDLR FVRLPVADSE ACQRWLQTKK DTSPFSQNMF CSGDPAVQQD 650
    ACQGDSGGVF AVRDRNRDIW VATGIVSWGI GCGEGYGFYT KVLNYVDWIK 700
    KEMGDEN 707
    Length:707
    Mass (Da):80,073
    Last modified:March 1, 2003 - v1
    Checksum:i29DAAEB3C047D8E8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti101 – 1011S → R in AAG01898. 1 PublicationCurated
    Sequence conflicti359 – 3591N → H in AAH04637. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148216 mRNA. Translation: AAG01898.1.
    AF459011
    , AF459008, AF459009, AF459010 Genomic DNA. Translation: AAO15553.1.
    BC004637 mRNA. Translation: AAH04637.1.
    CCDSiCCDS20521.1.
    RefSeqiNP_075632.3. NM_023143.3.
    UniGeneiMm.333375.

    Genome annotation databases

    EnsembliENSMUST00000068593; ENSMUSP00000063707; ENSMUSG00000055172.
    GeneIDi50909.
    KEGGimmu:50909.
    UCSCiuc009dra.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148216 mRNA. Translation: AAG01898.1 .
    AF459011
    , AF459008 , AF459009 , AF459010 Genomic DNA. Translation: AAO15553.1 .
    BC004637 mRNA. Translation: AAH04637.1 .
    CCDSi CCDS20521.1.
    RefSeqi NP_075632.3. NM_023143.3.
    UniGenei Mm.333375.

    3D structure databases

    ProteinModelPortali Q8CG16.
    SMRi Q8CG16. Positions 24-703.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000063707.

    Protein family/group databases

    MEROPSi S01.192.

    PTM databases

    PhosphoSitei Q8CG16.

    Proteomic databases

    PaxDbi Q8CG16.
    PRIDEi Q8CG16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000068593 ; ENSMUSP00000063707 ; ENSMUSG00000055172 .
    GeneIDi 50909.
    KEGGi mmu:50909.
    UCSCi uc009dra.2. mouse.

    Organism-specific databases

    CTDi 50909.
    MGIi MGI:1355313. C1ra.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00560000076882.
    HOGENOMi HOG000237311.
    HOVERGENi HBG000559.
    InParanoidi Q8CG16.
    KOi K01330.
    OMAi PRCLPVC.
    OrthoDBi EOG7W6WK4.
    PhylomeDBi Q8CG16.
    TreeFami TF330373.

    Miscellaneous databases

    NextBioi 307891.
    PROi Q8CG16.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8CG16.
    Bgeei Q8CG16.
    CleanExi MM_C1R.
    Genevestigatori Q8CG16.

    Family and domain databases

    Gene3Di 2.60.120.290. 2 hits.
    InterProi IPR000859. CUB_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000436. Sushi_SCR_CCP.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00431. CUB. 2 hits.
    PF00084. Sushi. 2 hits.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001155. C1r_C1s_MASP. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00032. CCP. 2 hits.
    SM00042. CUB. 2 hits.
    SM00179. EGF_CA. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49854. SSF49854. 2 hits.
    SSF50494. SSF50494. 1 hit.
    SSF57535. SSF57535. 2 hits.
    PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
    PS01180. CUB. 2 hits.
    PS01186. EGF_2. 1 hit.
    PS01187. EGF_CA. 1 hit.
    PS50923. SUSHI. 2 hits.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a cDNA encoding a serine protease homologous to human complement C1r precursor from an allografted mouse skin and its expression in Escherichia coli."
      Byun S.J., Hyun J.H., Hwang H.Y., Ryoo Z.Y., Kim T.Y.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system."
      Garnier G., Circolo A., Xu Y., Volanakis J.E.
      Biochem. J. 371:631-640(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: 129/SvJ.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-220.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiC1RA_MOUSE
    AccessioniPrimary (citable) accession number: Q8CG16
    Secondary accession number(s): Q99KI6, Q9ET60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3