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Q8CG14

- CS1A_MOUSE

UniProt

Q8CG14 - CS1A_MOUSE

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Protein
Complement C1s-A subcomponent
Gene
C1sa, C1s
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4 By similarity.

Catalytic activityi

Cleavage of Arg-|-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg)-|-Lys bond in complement component C2 to form C2a and C2b: the 'classical' pathway C3 convertase.

Enzyme regulationi

Inhibited by SERPING1 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Calcium By similarity
Metal bindingi68 – 681Calcium By similarity
Metal bindingi113 – 1131Calcium By similarity
Metal bindingi131 – 1311Calcium By similarity
Metal bindingi132 – 1321Calcium; via carbonyl oxygen By similarity
Metal bindingi134 – 1341Calcium By similarity
Metal bindingi149 – 1491Calcium By similarity
Metal bindingi150 – 1501Calcium; via carbonyl oxygen By similarity
Metal bindingi153 – 1531Calcium; via carbonyl oxygen By similarity
Active sitei475 – 4751Charge relay system By similarity
Active sitei529 – 5291Charge relay system By similarity
Active sitei631 – 6311Charge relay system By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. complement activation, classical pathway Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.360.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C1s-A subcomponent (EC:3.4.21.42)
Alternative name(s):
C1 esterase
Complement component 1 subcomponent s-A
Cleaved into the following 2 chains:
Gene namesi
Name:C1sa
Synonyms:C1s
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1355312. C1s.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515 By similarity
Add
BLAST
Chaini16 – 688673Complement C1s-A subcomponent
PRO_0000042193Add
BLAST
Chaini16 – 437422Complement C1s-A subcomponent heavy chain By similarity
PRO_0000042194Add
BLAST
Chaini438 – 688251Complement C1s-A subcomponent light chain By similarity
PRO_0000042195Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi65 ↔ 83 By similarity
Disulfide bondi135 ↔ 147 By similarity
Disulfide bondi143 ↔ 156 By similarity
Modified residuei149 – 1491(3R)-3-hydroxyasparagine By similarity
Disulfide bondi158 ↔ 171 By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi175 ↔ 202 By similarity
Disulfide bondi234 ↔ 251 By similarity
Disulfide bondi294 ↔ 341 By similarity
Disulfide bondi321 ↔ 354 By similarity
Disulfide bondi359 ↔ 403 By similarity
Disulfide bondi386 ↔ 421 By similarity
Disulfide bondi425 ↔ 549Interchain (between heavy and light chains) By similarity
Disulfide bondi595 ↔ 618 By similarity
Disulfide bondi627 ↔ 659 By similarity
Glycosylationi641 – 6411N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiQ8CG14.
PRIDEiQ8CG14.

PTM databases

PhosphoSiteiQ8CG14.

Expressioni

Tissue specificityi

Predominantly expressed in liver.1 Publication

Gene expression databases

CleanExiMM_C1S.
GenevestigatoriQ8CG14.

Interactioni

Subunit structurei

C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. Activated C1s is an disulfide-linked heterodimer of a heavy chain and a light chain By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ8CG14.
SMRiQ8CG14. Positions 17-683.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 130115CUB 1
Add
BLAST
Domaini131 – 17242EGF-like; calcium-binding Reviewed prediction
Add
BLAST
Domaini175 – 290116CUB 2
Add
BLAST
Domaini292 – 35665Sushi 1
Add
BLAST
Domaini357 – 42367Sushi 2
Add
BLAST
Domaini438 – 680243Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiCOG5640.
HOVERGENiHBG000559.
InParanoidiQ8CG14.
KOiK01331.
PhylomeDBiQ8CG14.

Family and domain databases

Gene3Di2.60.120.290. 2 hits.
InterProiIPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG14-1 [UniParc]FASTAAdd to Basket

« Hide

MWCLVLFSLL ASFSAEPTMH GEILSPNYPQ AYPNDVVKSW DIEVPEGFGI    50
HLYFTHVDIE PSESCAYDSV QIISGGIEEG RLCGQKTSKS PNSPIIEEFQ 100
FPYNKLQVVF TSDFSNEERF TGFAAYYTAI DINECTDFTD VPCSHFCNNF 150
IGGYFCSCPP EYFLHDDMRN CGVNCSGDVF TALIGEISSP NYPNPYPENS 200
RCEYQIQLQE GFQVVVTMQR EDFDVEPADS EGNCPDSLTF ASKNQQFGPY 250
CGNGFPGPLT IRTQSNTLGI VFQTDLMGQK KGWKLRYHGD PISCAKKITA 300
NSTWEPDKAK YVFKDVVKIT CVDGFEVVEG HVSSTSYYST CQSDGQWSNS 350
GLKCQPVYCG IPDPIANGKV EEPENSVFGT VVHYTCEEPY YYMEHEEGGE 400
YRCAANGRWV NDQLGIELPR CIPACGVPTE PFQVHQRIFG GQPAKIENFP 450
WQVFFNHPRA SGALINEYWV LTAAHVLEKI SDPLMYVGTM SVRTTLLENA 500
QRLYSKRVFI HPSWKKEDDP NTRTNFDNDI ALVQLKDPVK MGPKVSPICL 550
PGTSSEYNVS PGDMGLISGW GSTEKKVFVI NLRGAKVPVT SLETCKQVKE 600
ENPTVRPEDY VFTDNMICAG EKGVDSCHGD SGGAFAFQVP NVTVPKFYVA 650
GLVSWGKRCG TYGVYTKVKN YVDWILKTMQ ENSGPRKD 688
Length:688
Mass (Da):76,858
Last modified:September 27, 2005 - v2
Checksum:iBAC166C861CB8A25
GO

Sequence cautioni

The sequence BAC39910.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761G → D in AAO15556. 1 Publication
Sequence conflicti76 – 761G → D in BAC39910. 1 Publication
Sequence conflicti86 – 861K → R in AAO15556. 1 Publication
Sequence conflicti86 – 861K → R in BAC39910. 1 Publication
Sequence conflicti305 – 3051E → Q in AAO15556. 1 Publication
Sequence conflicti305 – 3051E → Q in BAC39910. 1 Publication
Sequence conflicti378 – 3781F → L in BAC39910. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF459019 mRNA. Translation: AAO15558.1.
AF459017, AF459015, AF459016 Genomic DNA. Translation: AAO15556.1.
AK087522 mRNA. Translation: BAC39910.1. Different initiation.
BC022123 mRNA. Translation: AAH22123.1.
BC018319 mRNA. Translation: AAH18319.1.
RefSeqiNP_001091086.1. NM_001097617.1.
NP_659187.2. NM_144938.2.
UniGeneiMm.219527.

Genome annotation databases

GeneIDi50908.
KEGGimmu:50908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF459019 mRNA. Translation: AAO15558.1 .
AF459017 , AF459015 , AF459016 Genomic DNA. Translation: AAO15556.1 .
AK087522 mRNA. Translation: BAC39910.1 . Different initiation.
BC022123 mRNA. Translation: AAH22123.1 .
BC018319 mRNA. Translation: AAH18319.1 .
RefSeqi NP_001091086.1. NM_001097617.1.
NP_659187.2. NM_144938.2.
UniGenei Mm.219527.

3D structure databases

ProteinModelPortali Q8CG14.
SMRi Q8CG14. Positions 17-683.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.360.

PTM databases

PhosphoSitei Q8CG14.

Proteomic databases

PaxDbi Q8CG14.
PRIDEi Q8CG14.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 50908.
KEGGi mmu:50908.

Organism-specific databases

CTDi 50908.
MGIi MGI:1355312. C1s.

Phylogenomic databases

eggNOGi COG5640.
HOVERGENi HBG000559.
InParanoidi Q8CG14.
KOi K01331.
PhylomeDBi Q8CG14.

Miscellaneous databases

NextBioi 307885.
PROi Q8CG14.
SOURCEi Search...

Gene expression databases

CleanExi MM_C1S.
Genevestigatori Q8CG14.

Family and domain databases

Gene3Di 2.60.120.290. 2 hits.
InterProi IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024175. Pept_S1A_C1r/C1S/mannan-bd.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR000436. Sushi_SCR_CCP.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00431. CUB. 2 hits.
PF00084. Sushi. 2 hits.
PF00089. Trypsin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001155. C1r_C1s_MASP. 1 hit.
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00032. CCP. 2 hits.
SM00042. CUB. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF49854. SSF49854. 2 hits.
SSF50494. SSF50494. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS01180. CUB. 2 hits.
PS01187. EGF_CA. 1 hit.
PS50923. SUSHI. 2 hits.
PS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complement C1r and C1s genes are duplicated in the mouse: differential expression generates alternative isomorphs in the liver and in the male reproductive system."
    Garnier G., Circolo A., Xu Y., Volanakis J.E.
    Biochem. J. 371:631-640(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
    Strain: 129/SvJ and BALB/c.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiCS1A_MOUSE
AccessioniPrimary (citable) accession number: Q8CG14
Secondary accession number(s): Q8BJC4, Q8CH28, Q8VBY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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