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Protein

Multidrug resistance-associated protein 1

Gene

Abcc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi679 – 686ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1328 – 1335ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • drug transmembrane transporter activity Source: RGD
  • efflux transmembrane transporter activity Source: RGD
  • glutathione S-conjugate-exporting ATPase activity Source: RGD
  • glutathione transmembrane transporter activity Source: RGD
  • lipid-transporting ATPase activity Source: RGD
  • long-chain fatty acid transporter activity Source: RGD
  • oxidoreductase activity Source: InterPro
  • sphingolipid transporter activity Source: RGD
  • xenobiotic-transporting ATPase activity Source: RGD

GO - Biological processi

  • cell chemotaxis Source: RGD
  • daunorubicin transport Source: RGD
  • drug export Source: RGD
  • drug transmembrane transport Source: RGD
  • glutathione transmembrane transport Source: RGD
  • negative regulation of neuron death Source: RGD
  • phospholipid efflux Source: RGD
  • plasma membrane long-chain fatty acid transport Source: RGD
  • positive regulation of cell migration Source: RGD
  • response to drug Source: RGD
  • response to oxidative stress Source: RGD
  • transmembrane transport Source: RGD
  • xenobiotic transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Chemistry databases

SwissLipidsiSLP:000000405.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance-associated protein 1
Alternative name(s):
ATP-binding cassette sub-family C member 1
Leukotriene C(4) transporter
Short name:
LTC4 transporter
Gene namesi
Name:Abcc1
Synonyms:Mrp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3112. Abcc1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33ExtracellularBy similarityAdd BLAST33
Transmembranei34 – 54Helical; Name=1PROSITE-ProRule annotationAdd BLAST21
Topological domaini55 – 74CytoplasmicBy similarityAdd BLAST20
Transmembranei75 – 95Helical; Name=2PROSITE-ProRule annotationAdd BLAST21
Topological domaini96 – 100ExtracellularBy similarity5
Transmembranei101 – 121Helical; Name=3PROSITE-ProRule annotationAdd BLAST21
Topological domaini122 – 133CytoplasmicBy similarityAdd BLAST12
Transmembranei134 – 154Helical; Name=4PROSITE-ProRule annotationAdd BLAST21
Topological domaini155 – 172ExtracellularBy similarityAdd BLAST18
Transmembranei173 – 193Helical; Name=5PROSITE-ProRule annotationAdd BLAST21
Topological domaini194 – 317CytoplasmicBy similarityAdd BLAST124
Transmembranei318 – 338Helical; Name=6PROSITE-ProRule annotationAdd BLAST21
Topological domaini339 – 364ExtracellularBy similarityAdd BLAST26
Transmembranei365 – 385Helical; Name=7PROSITE-ProRule annotationAdd BLAST21
Topological domaini386 – 441CytoplasmicBy similarityAdd BLAST56
Transmembranei442 – 462Helical; Name=8PROSITE-ProRule annotationAdd BLAST21
Topological domaini463 – 465ExtracellularBy similarity3
Transmembranei466 – 486Helical; Name=9PROSITE-ProRule annotationAdd BLAST21
Topological domaini487 – 548CytoplasmicBy similarityAdd BLAST62
Transmembranei549 – 569Helical; Name=10PROSITE-ProRule annotationAdd BLAST21
Topological domaini570 – 591ExtracellularBy similarityAdd BLAST22
Transmembranei592 – 612Helical; Name=11PROSITE-ProRule annotationAdd BLAST21
Topological domaini613 – 967CytoplasmicBy similarityAdd BLAST355
Transmembranei968 – 988Helical; Name=12PROSITE-ProRule annotationAdd BLAST21
Topological domaini989 – 1026ExtracellularBy similarityAdd BLAST38
Transmembranei1027 – 1047Helical; Name=13PROSITE-ProRule annotationAdd BLAST21
Topological domaini1048 – 1090CytoplasmicBy similarityAdd BLAST43
Transmembranei1091 – 1111Helical; Name=14PROSITE-ProRule annotationAdd BLAST21
Topological domaini1112ExtracellularBy similarity1
Transmembranei1113 – 1133Helical; Name=15PROSITE-ProRule annotationAdd BLAST21
Topological domaini1134 – 1204CytoplasmicBy similarityAdd BLAST71
Transmembranei1205 – 1225Helical; Name=16PROSITE-ProRule annotationAdd BLAST21
Topological domaini1226 – 1227ExtracellularBy similarity2
Transmembranei1228 – 1248Helical; Name=17PROSITE-ProRule annotationAdd BLAST21
Topological domaini1249 – 1532CytoplasmicBy similarityAdd BLAST284

GO - Cellular componenti

  • basolateral plasma membrane Source: CACAO
  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: RGD
  • membrane Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi983L → M: No effect on estradiol glucuronide transport. 1 Publication1
Mutagenesisi1090Q → E: 7.6-fold increase of the estradiol glucuronide transport; when associated with T-1243. Increases doxorubicin inhibition. 1 Publication1
Mutagenesisi1101S → N: 50% increase of estradiol glucuronide transport. 1 Publication1
Mutagenesisi1106V → C: No effect on estradiol glucuronide transport. 1 Publication1
Mutagenesisi1243A → T: 7.6-fold increase of the estradiol glucuronide transport; when associated with E-1090. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5680.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933541 – 1532Multidrug resistance-associated protein 1Add BLAST1532

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi19N-linked (GlcNAc...)Sequence analysis1
Modified residuei277PhosphotyrosineBy similarity1
Modified residuei290PhosphoserineBy similarity1
Modified residuei504N6-succinyllysineBy similarity1
Modified residuei879PhosphoserineCombined sources1
Modified residuei883PhosphoserineBy similarity1
Modified residuei916PhosphoserineBy similarity1
Modified residuei931PhosphoserineBy similarity1

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8CG09.
PRIDEiQ8CG09.

PTM databases

iPTMnetiQ8CG09.
PhosphoSitePlusiQ8CG09.

Expressioni

Tissue specificityi

Skeletal muscle, brain, heart, spleen, lung and kidney.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041695.

Structurei

3D structure databases

ProteinModelPortaliQ8CG09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini326 – 609ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST284
Domaini645 – 869ABC transporter 1PROSITE-ProRule annotationAdd BLAST225
Domaini975 – 1257ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST283
Domaini1294 – 1528ABC transporter 2PROSITE-ProRule annotationAdd BLAST235

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG108314.
InParanoidiQ8CG09.
KOiK05665.
PhylomeDBiQ8CG09.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030241. ABCC1.
IPR018170. Aldo/ket_reductase_CS.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24223:SF241. PTHR24223:SF241. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CG09-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALSSFCSSD GSDPLWDWNV TWHTSNPDFT KCFQNTVLTW VPCFYLWSCF
60 70 80 90 100
PLYFLYLSRH DRGYIQMTHL NKAKTALGFF LWIICWADLF YSFWERSQGM
110 120 130 140 150
LLAPVLLVSP TLLGITMLLA TFLIQFERRK GVQSSGIMLT FWLVALLCAL
160 170 180 190 200
AILRSKIISA LKKDAQVDMF RDSAFYLYFT LVFIQLVLSC FSDSSPLFSE
210 220 230 240 250
TVRDPNPCPE SSASFLSRIT FWWITGMMVQ GYRQPLKSSD LWSLNKEDTS
260 270 280 290 300
EEVVPVLVNN WKKECVKSRK QPVRIVYAPP KDPTKPKGSS QLDVNEEVEA
310 320 330 340 350
LIVKSSHKDR DPSLFKVLYK TFGPYFLMSF LYKALHDLMM FAGPEILELI
360 370 380 390 400
INFVNDREAP DWQGYLYTAL LFVSACLQTL ALHQYFHICF VTGMRIKTAV
410 420 430 440 450
VGAVYRKALV ITNSARKSST VGEIVNLMSV DAQRFMDLAT YINMIWSAPL
460 470 480 490 500
QVTLALYFLW LNLGPSVLAG VAVMILMVPF NAVMAMKTKT YQVAHMKSKD
510 520 530 540 550
NRIKLMNEIL NGIKVLKLYA WELAFQDKVM NIRQEELKVL KKSAYLAAVG
560 570 580 590 600
TFTWVCTPFL VALSTFAVFV TVDEKNILDA KKAFVSLALF NILRFPLNIL
610 620 630 640 650
PMVISSIVQA SVSLKRLRIF LSHEELEPDS IERWSIKDGG GMNSITVKNA
660 670 680 690 700
TFTWARDEPP TLNGITFAIP DGALVAVVGQ VGCGKSSLLS ALLAEMDKVE
710 720 730 740 750
GHVTLKGSVA YVPQQAWIQN DSLRENILFG RPLQEHCYKA VMEACALLPD
760 770 780 790 800
LEILPSGDLT EIGEKGVNLS GGQKQRVSLA RAVYCNSDIY LLDDPLSAVD
810 820 830 840 850
AHVGKHIFEK VVGPMGLLKN KTRILVTHGI SYLPQVDVII VMSGGKISEM
860 870 880 890 900
GSYQELLDRD GAFAEFVRTY ANTEQDLASE DDSKNGVSGL GKESKPVENG
910 920 930 940 950
ILVTDAVGKP LQRHLSNSSS HSVVTNQQHS STAELQKSGV KEETWKLMEA
960 970 980 990 1000
DKAQTGQVKL SVYWNYMKAI GLCISFLSIF LFLCNHVSAL ASNYWLSLWT
1010 1020 1030 1040 1050
DDRPAVNGTQ ENRNFRLSVY GALGILQGVA VFGYSMAVSI GGIFASRRLH
1060 1070 1080 1090 1100
LDLLQNVLRS PMSFFERTPS GNLVNRFSKE LDTVDSMIPQ VIKMFMGSLF
1110 1120 1130 1140 1150
SVIGAVIIIL LATPIAAVII PPLGLVYFFV QRFYVASSRQ LKRLESVSRS
1160 1170 1180 1190 1200
PVYSHFNETL LGVSVIRAFE EQERFIRQSD LKVDENQKAY YPSIVANRWL
1210 1220 1230 1240 1250
AVRLECVGNC IVLFAALFAV ISRHSLSAGL VGLSVSYSLQ ITAYLNWLVR
1260 1270 1280 1290 1300
MSSEMETNIV AVERLKEYSE TEKEASWQIQ ETAPPSTWPH SGRVEFRDYC
1310 1320 1330 1340 1350
LRYREDLDLV LKHINVTIEG GEKVGIVGRT GAGKSSLTLG LFRINESAEG
1360 1370 1380 1390 1400
EIIIDGINIA KIGLHNLRFK ITIIPQDPVL FSGSLRMNLD PFSQYSDEEV
1410 1420 1430 1440 1450
WMALELAHLK GFVSALPDKL NHECAEGGEN LSVGQRQLVC LARALLRKTK
1460 1470 1480 1490 1500
ILVLDEATAA VDLETDDLIQ STIRTQFEDS TVLTIAHRLN TIMDYTRVIV
1510 1520 1530
LDKGEIRECG APSELLQQRG VFYSMAKDAG LV
Length:1,532
Mass (Da):171,493
Last modified:January 24, 2006 - v2
Checksum:i2E6939F63F5A3F68
GO
Isoform 2 (identifier: Q8CG09-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     912-920: Missing.

Note: No experimental confirmation available.
Show »
Length:1,523
Mass (Da):170,496
Checksum:i15FB4E9C99B7B9AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4S → R in AAN86532 (PubMed:12423064).Curated1
Sequence conflicti4S → R in AAO44983 (Ref. 4) Curated1
Sequence conflicti480F → S in AAO44983 (Ref. 4) Curated1
Sequence conflicti820N → S in CAB97204 (PubMed:11208926).Curated1
Sequence conflicti868R → P in CAB97204 (PubMed:11208926).Curated1
Sequence conflicti916S → G in CAB97204 (PubMed:11208926).Curated1
Sequence conflicti1122P → T in CAB97204 (PubMed:11208926).Curated1
Sequence conflicti1382S → P in AAN86532 (PubMed:12423064).Curated1
Sequence conflicti1473I → V in AAN86532 (PubMed:12423064).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017015912 – 920Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY170916 mRNA. Translation: AAN86532.1.
AF487549 mRNA. Translation: AAO85437.1.
AY174892 mRNA. Translation: AAO44983.1.
AJ277881 mRNA. Translation: CAB97204.1.
X96394 mRNA. Translation: CAA65258.1.
RefSeqiNP_071617.2. NM_022281.2. [Q8CG09-1]
UniGeneiRn.10495.

Genome annotation databases

GeneIDi24565.
KEGGirno:24565.
UCSCiRGD:3112. rat. [Q8CG09-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY170916 mRNA. Translation: AAN86532.1.
AF487549 mRNA. Translation: AAO85437.1.
AY174892 mRNA. Translation: AAO44983.1.
AJ277881 mRNA. Translation: CAB97204.1.
X96394 mRNA. Translation: CAA65258.1.
RefSeqiNP_071617.2. NM_022281.2. [Q8CG09-1]
UniGeneiRn.10495.

3D structure databases

ProteinModelPortaliQ8CG09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041695.

Chemistry databases

ChEMBLiCHEMBL5680.
SwissLipidsiSLP:000000405.

PTM databases

iPTMnetiQ8CG09.
PhosphoSitePlusiQ8CG09.

Proteomic databases

PaxDbiQ8CG09.
PRIDEiQ8CG09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24565.
KEGGirno:24565.
UCSCiRGD:3112. rat. [Q8CG09-1]

Organism-specific databases

CTDi4363.
RGDi3112. Abcc1.

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG108314.
InParanoidiQ8CG09.
KOiK05665.
PhylomeDBiQ8CG09.

Miscellaneous databases

PROiQ8CG09.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030241. ABCC1.
IPR018170. Aldo/ket_reductase_CS.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24223:SF241. PTHR24223:SF241. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMRP1_RAT
AccessioniPrimary (citable) accession number: Q8CG09
Secondary accession number(s): Q63346
, Q810E4, Q810G9, Q9JHS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.