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Q8CG09

- MRP1_RAT

UniProt

Q8CG09 - MRP1_RAT

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Protein

Multidrug resistance-associated protein 1

Gene

Abcc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi679 – 6868ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1328 – 13358ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. drug transmembrane transporter activity Source: RGD
  3. efflux transmembrane transporter activity Source: RGD
  4. glutathione S-conjugate-exporting ATPase activity Source: RGD
  5. glutathione transmembrane transporter activity Source: RGD
  6. lipid-transporting ATPase activity Source: RGD
  7. long-chain fatty acid transporter activity Source: RGD
  8. oxidoreductase activity Source: InterPro
  9. sphingolipid transporter activity Source: RGD
  10. xenobiotic-transporting ATPase activity Source: RGD

GO - Biological processi

  1. cell chemotaxis Source: RGD
  2. daunorubicin transport Source: RGD
  3. drug export Source: RGD
  4. drug transmembrane transport Source: RGD
  5. glutathione transmembrane transport Source: RGD
  6. negative regulation of neuron death Source: RGD
  7. phospholipid efflux Source: RGD
  8. plasma membrane long-chain fatty acid transport Source: RGD
  9. positive regulation of cell migration Source: RGD
  10. response to drug Source: RGD
  11. response to oxidative stress Source: RGD
  12. transmembrane transport Source: RGD
  13. xenobiotic transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance-associated protein 1
Alternative name(s):
ATP-binding cassette sub-family C member 1
Leukotriene C(4) transporter
Short name:
LTC4 transporter
Gene namesi
Name:Abcc1
Synonyms:Mrp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3112. Abcc1.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. basolateral plasma membrane Source: RGD
  2. cytoplasm Source: RGD
  3. integral component of plasma membrane Source: RGD
  4. membrane Source: RGD
  5. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi983 – 9831L → M: No effect on estradiol glucuronide transport. 1 Publication
Mutagenesisi1090 – 10901Q → E: 7.6-fold increase of the estradiol glucuronide transport; when associated with T-1243. Increases doxorubicin inhibition. 1 Publication
Mutagenesisi1101 – 11011S → N: 50% increase of estradiol glucuronide transport. 1 Publication
Mutagenesisi1106 – 11061V → C: No effect on estradiol glucuronide transport. 1 Publication
Mutagenesisi1243 – 12431A → T: 7.6-fold increase of the estradiol glucuronide transport; when associated with E-1090. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15321532Multidrug resistance-associated protein 1PRO_0000093354Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi19 – 191N-linked (GlcNAc...)Sequence Analysis
Modified residuei504 – 5041N6-succinyllysineBy similarity
Modified residuei879 – 8791PhosphoserineBy similarity
Modified residuei883 – 8831PhosphoserineBy similarity
Modified residuei916 – 9161PhosphoserineBy similarity
Modified residuei931 – 9311PhosphoserineBy similarity

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8CG09.
PRIDEiQ8CG09.

PTM databases

PhosphoSiteiQ8CG09.

Expressioni

Tissue specificityi

Skeletal muscle, brain, heart, spleen, lung and kidney.1 Publication

Gene expression databases

GenevestigatoriQ8CG09.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045518.

Structurei

3D structure databases

ProteinModelPortaliQ8CG09.
SMRiQ8CG09. Positions 643-872.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333ExtracellularBy similarityAdd
BLAST
Topological domaini55 – 7420CytoplasmicBy similarityAdd
BLAST
Topological domaini96 – 1005ExtracellularBy similarity
Topological domaini122 – 13312CytoplasmicBy similarityAdd
BLAST
Topological domaini155 – 17218ExtracellularBy similarityAdd
BLAST
Topological domaini194 – 317124CytoplasmicBy similarityAdd
BLAST
Topological domaini339 – 36426ExtracellularBy similarityAdd
BLAST
Topological domaini386 – 44156CytoplasmicBy similarityAdd
BLAST
Topological domaini463 – 4653ExtracellularBy similarity
Topological domaini487 – 54862CytoplasmicBy similarityAdd
BLAST
Topological domaini570 – 59122ExtracellularBy similarityAdd
BLAST
Topological domaini613 – 967355CytoplasmicBy similarityAdd
BLAST
Topological domaini989 – 102638ExtracellularBy similarityAdd
BLAST
Topological domaini1048 – 109043CytoplasmicBy similarityAdd
BLAST
Topological domaini1112 – 11121ExtracellularBy similarity
Topological domaini1134 – 120471CytoplasmicBy similarityAdd
BLAST
Topological domaini1226 – 12272ExtracellularBy similarity
Topological domaini1249 – 1532284CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei34 – 5421Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Transmembranei75 – 9521Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Transmembranei101 – 12121Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Transmembranei134 – 15421Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Transmembranei173 – 19321Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Transmembranei318 – 33821Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Transmembranei365 – 38521Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Transmembranei442 – 46221Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Transmembranei466 – 48621Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Transmembranei549 – 56921Helical; Name=10PROSITE-ProRule annotationAdd
BLAST
Transmembranei592 – 61221Helical; Name=11PROSITE-ProRule annotationAdd
BLAST
Transmembranei968 – 98821Helical; Name=12PROSITE-ProRule annotationAdd
BLAST
Transmembranei1027 – 104721Helical; Name=13PROSITE-ProRule annotationAdd
BLAST
Transmembranei1091 – 111121Helical; Name=14PROSITE-ProRule annotationAdd
BLAST
Transmembranei1113 – 113321Helical; Name=15PROSITE-ProRule annotationAdd
BLAST
Transmembranei1205 – 122521Helical; Name=16PROSITE-ProRule annotationAdd
BLAST
Transmembranei1228 – 124821Helical; Name=17PROSITE-ProRule annotationAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini326 – 609284ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini645 – 869225ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini975 – 1257283ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1294 – 1528235ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
HOVERGENiHBG108314.
InParanoidiQ8CG09.
KOiK05665.
PhylomeDBiQ8CG09.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR018170. Aldo/ket_reductase_CS.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8CG09-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALSSFCSSD GSDPLWDWNV TWHTSNPDFT KCFQNTVLTW VPCFYLWSCF
60 70 80 90 100
PLYFLYLSRH DRGYIQMTHL NKAKTALGFF LWIICWADLF YSFWERSQGM
110 120 130 140 150
LLAPVLLVSP TLLGITMLLA TFLIQFERRK GVQSSGIMLT FWLVALLCAL
160 170 180 190 200
AILRSKIISA LKKDAQVDMF RDSAFYLYFT LVFIQLVLSC FSDSSPLFSE
210 220 230 240 250
TVRDPNPCPE SSASFLSRIT FWWITGMMVQ GYRQPLKSSD LWSLNKEDTS
260 270 280 290 300
EEVVPVLVNN WKKECVKSRK QPVRIVYAPP KDPTKPKGSS QLDVNEEVEA
310 320 330 340 350
LIVKSSHKDR DPSLFKVLYK TFGPYFLMSF LYKALHDLMM FAGPEILELI
360 370 380 390 400
INFVNDREAP DWQGYLYTAL LFVSACLQTL ALHQYFHICF VTGMRIKTAV
410 420 430 440 450
VGAVYRKALV ITNSARKSST VGEIVNLMSV DAQRFMDLAT YINMIWSAPL
460 470 480 490 500
QVTLALYFLW LNLGPSVLAG VAVMILMVPF NAVMAMKTKT YQVAHMKSKD
510 520 530 540 550
NRIKLMNEIL NGIKVLKLYA WELAFQDKVM NIRQEELKVL KKSAYLAAVG
560 570 580 590 600
TFTWVCTPFL VALSTFAVFV TVDEKNILDA KKAFVSLALF NILRFPLNIL
610 620 630 640 650
PMVISSIVQA SVSLKRLRIF LSHEELEPDS IERWSIKDGG GMNSITVKNA
660 670 680 690 700
TFTWARDEPP TLNGITFAIP DGALVAVVGQ VGCGKSSLLS ALLAEMDKVE
710 720 730 740 750
GHVTLKGSVA YVPQQAWIQN DSLRENILFG RPLQEHCYKA VMEACALLPD
760 770 780 790 800
LEILPSGDLT EIGEKGVNLS GGQKQRVSLA RAVYCNSDIY LLDDPLSAVD
810 820 830 840 850
AHVGKHIFEK VVGPMGLLKN KTRILVTHGI SYLPQVDVII VMSGGKISEM
860 870 880 890 900
GSYQELLDRD GAFAEFVRTY ANTEQDLASE DDSKNGVSGL GKESKPVENG
910 920 930 940 950
ILVTDAVGKP LQRHLSNSSS HSVVTNQQHS STAELQKSGV KEETWKLMEA
960 970 980 990 1000
DKAQTGQVKL SVYWNYMKAI GLCISFLSIF LFLCNHVSAL ASNYWLSLWT
1010 1020 1030 1040 1050
DDRPAVNGTQ ENRNFRLSVY GALGILQGVA VFGYSMAVSI GGIFASRRLH
1060 1070 1080 1090 1100
LDLLQNVLRS PMSFFERTPS GNLVNRFSKE LDTVDSMIPQ VIKMFMGSLF
1110 1120 1130 1140 1150
SVIGAVIIIL LATPIAAVII PPLGLVYFFV QRFYVASSRQ LKRLESVSRS
1160 1170 1180 1190 1200
PVYSHFNETL LGVSVIRAFE EQERFIRQSD LKVDENQKAY YPSIVANRWL
1210 1220 1230 1240 1250
AVRLECVGNC IVLFAALFAV ISRHSLSAGL VGLSVSYSLQ ITAYLNWLVR
1260 1270 1280 1290 1300
MSSEMETNIV AVERLKEYSE TEKEASWQIQ ETAPPSTWPH SGRVEFRDYC
1310 1320 1330 1340 1350
LRYREDLDLV LKHINVTIEG GEKVGIVGRT GAGKSSLTLG LFRINESAEG
1360 1370 1380 1390 1400
EIIIDGINIA KIGLHNLRFK ITIIPQDPVL FSGSLRMNLD PFSQYSDEEV
1410 1420 1430 1440 1450
WMALELAHLK GFVSALPDKL NHECAEGGEN LSVGQRQLVC LARALLRKTK
1460 1470 1480 1490 1500
ILVLDEATAA VDLETDDLIQ STIRTQFEDS TVLTIAHRLN TIMDYTRVIV
1510 1520 1530
LDKGEIRECG APSELLQQRG VFYSMAKDAG LV
Length:1,532
Mass (Da):171,493
Last modified:January 24, 2006 - v2
Checksum:i2E6939F63F5A3F68
GO
Isoform 2 (identifier: Q8CG09-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     912-920: Missing.

Note: No experimental confirmation available.

Show »
Length:1,523
Mass (Da):170,496
Checksum:i15FB4E9C99B7B9AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41S → R in AAN86532. (PubMed:12423064)Curated
Sequence conflicti4 – 41S → R in AAO44983. 1 PublicationCurated
Sequence conflicti480 – 4801F → S in AAO44983. 1 PublicationCurated
Sequence conflicti820 – 8201N → S in CAB97204. (PubMed:11208926)Curated
Sequence conflicti868 – 8681R → P in CAB97204. (PubMed:11208926)Curated
Sequence conflicti916 – 9161S → G in CAB97204. (PubMed:11208926)Curated
Sequence conflicti1122 – 11221P → T in CAB97204. (PubMed:11208926)Curated
Sequence conflicti1382 – 13821S → P in AAN86532. (PubMed:12423064)Curated
Sequence conflicti1473 – 14731I → V in AAN86532. (PubMed:12423064)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei912 – 9209Missing in isoform 2. 1 PublicationVSP_017015

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY170916 mRNA. Translation: AAN86532.1.
AF487549 mRNA. Translation: AAO85437.1.
AY174892 mRNA. Translation: AAO44983.1.
AJ277881 mRNA. Translation: CAB97204.1.
X96394 mRNA. Translation: CAA65258.1.
RefSeqiNP_071617.2. NM_022281.2. [Q8CG09-1]
UniGeneiRn.10495.

Genome annotation databases

GeneIDi24565.
KEGGirno:24565.
UCSCiRGD:3112. rat. [Q8CG09-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY170916 mRNA. Translation: AAN86532.1 .
AF487549 mRNA. Translation: AAO85437.1 .
AY174892 mRNA. Translation: AAO44983.1 .
AJ277881 mRNA. Translation: CAB97204.1 .
X96394 mRNA. Translation: CAA65258.1 .
RefSeqi NP_071617.2. NM_022281.2. [Q8CG09-1 ]
UniGenei Rn.10495.

3D structure databases

ProteinModelPortali Q8CG09.
SMRi Q8CG09. Positions 643-872.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000045518.

Chemistry

ChEMBLi CHEMBL5680.

PTM databases

PhosphoSitei Q8CG09.

Proteomic databases

PaxDbi Q8CG09.
PRIDEi Q8CG09.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24565.
KEGGi rno:24565.
UCSCi RGD:3112. rat. [Q8CG09-1 ]

Organism-specific databases

CTDi 4363.
RGDi 3112. Abcc1.

Phylogenomic databases

eggNOGi COG1132.
HOVERGENi HBG108314.
InParanoidi Q8CG09.
KOi K05665.
PhylomeDBi Q8CG09.

Miscellaneous databases

NextBioi 603696.
PROi Q8CG09.

Gene expression databases

Genevestigatori Q8CG09.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR018170. Aldo/ket_reductase_CS.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsi TIGR00957. MRP_assoc_pro. 1 hit.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the rat multidrug resistance-associated protein 1."
    Yang Z., Li C.S.W., Shen D.D., Ho R.J.Y.
    AAPS PharmSci 4:E15-E15(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Molecular cloning and pharmacological characterization of rat multidrug resistance protein 1 (mrp1)."
    Nunoya K., Grant C.E., Zhang D.-W., Cole S.P.C., Deeley R.G.
    Drug Metab. Dispos. 31:1016-1026(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, MUTAGENESIS OF LEU-983; GLN-1090; SER-1101; VAL-1106 AND ALA-1243.
    Strain: Sprague-Dawley.
    Tissue: Skeletal muscle.
  3. Yabuuchi H., Takayanagi S., Ishikawa T.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Spleen.
  4. Takayanagi S., Ishikawa T.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Sprague-Dawley.
    Tissue: Spleen.
  5. "The multidrug resistance protein MRP1 mediates the release of glutathione disulfide from rat astrocytes during oxidative stress."
    Hirrlinger J., Koenig J., Keppler D., Lindenau J., Schulz J.B., Dringen R.
    J. Neurochem. 76:627-636(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 711-1532 (ISOFORM 1).
  6. "cDNA cloning of the hepatocyte canalicular isoform of the multidrug resistance protein, cMrp, reveals a novel conjugate export pump deficient in hyperbilirubinemic mutant rats."
    Buechler M., Koenig J., Brom M., Kartenbeck J., Spring H., Horie T., Keppler D.
    J. Biol. Chem. 271:15091-15098(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1426-1532 (ISOFORMS 1/2).
  7. "Multidrug resistance protein 1-mediated transport of saquinavir by microglia."
    Dallas S., Ronaldson P.T., Bendayan M., Bendayan R.
    NeuroReport 15:1183-1186(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMRP1_RAT
AccessioniPrimary (citable) accession number: Q8CG09
Secondary accession number(s): Q63346
, Q810E4, Q810G9, Q9JHS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3