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Q8CG09

- MRP1_RAT

UniProt

Q8CG09 - MRP1_RAT

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Protein

Multidrug resistance-associated protein 1

Gene
Abcc1, Mrp1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi679 – 6868ATP 1 Reviewed prediction
Nucleotide bindingi1328 – 13358ATP 2 Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. drug transmembrane transporter activity Source: RGD
  3. efflux transmembrane transporter activity Source: RGD
  4. glutathione S-conjugate-exporting ATPase activity Source: RGD
  5. glutathione transmembrane transporter activity Source: RGD
  6. lipid-transporting ATPase activity Source: RGD
  7. long-chain fatty acid transporter activity Source: RGD
  8. oxidoreductase activity Source: InterPro
  9. sphingolipid transporter activity Source: RGD
  10. xenobiotic-transporting ATPase activity Source: RGD

GO - Biological processi

  1. cell chemotaxis Source: RGD
  2. daunorubicin transport Source: RGD
  3. drug export Source: RGD
  4. drug transmembrane transport Source: RGD
  5. glutathione transmembrane transport Source: RGD
  6. negative regulation of neuron death Source: RGD
  7. phospholipid efflux Source: RGD
  8. plasma membrane long-chain fatty acid transport Source: RGD
  9. positive regulation of cell migration Source: RGD
  10. response to drug Source: RGD
  11. response to oxidative stress Source: RGD
  12. transmembrane transport Source: RGD
  13. xenobiotic transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance-associated protein 1
Alternative name(s):
ATP-binding cassette sub-family C member 1
Leukotriene C(4) transporter
Short name:
LTC4 transporter
Gene namesi
Name:Abcc1
Synonyms:Mrp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3112. Abcc1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333Extracellular By similarityAdd
BLAST
Transmembranei34 – 5421Helical; Name=1; By similarityAdd
BLAST
Topological domaini55 – 7420Cytoplasmic By similarityAdd
BLAST
Transmembranei75 – 9521Helical; Name=2; By similarityAdd
BLAST
Topological domaini96 – 1005Extracellular By similarity
Transmembranei101 – 12121Helical; Name=3; By similarityAdd
BLAST
Topological domaini122 – 13312Cytoplasmic By similarityAdd
BLAST
Transmembranei134 – 15421Helical; Name=4; By similarityAdd
BLAST
Topological domaini155 – 17218Extracellular By similarityAdd
BLAST
Transmembranei173 – 19321Helical; Name=5; By similarityAdd
BLAST
Topological domaini194 – 317124Cytoplasmic By similarityAdd
BLAST
Transmembranei318 – 33821Helical; Name=6; By similarityAdd
BLAST
Topological domaini339 – 36426Extracellular By similarityAdd
BLAST
Transmembranei365 – 38521Helical; Name=7; By similarityAdd
BLAST
Topological domaini386 – 44156Cytoplasmic By similarityAdd
BLAST
Transmembranei442 – 46221Helical; Name=8; By similarityAdd
BLAST
Topological domaini463 – 4653Extracellular By similarity
Transmembranei466 – 48621Helical; Name=9; By similarityAdd
BLAST
Topological domaini487 – 54862Cytoplasmic By similarityAdd
BLAST
Transmembranei549 – 56921Helical; Name=10; By similarityAdd
BLAST
Topological domaini570 – 59122Extracellular By similarityAdd
BLAST
Transmembranei592 – 61221Helical; Name=11; By similarityAdd
BLAST
Topological domaini613 – 967355Cytoplasmic By similarityAdd
BLAST
Transmembranei968 – 98821Helical; Name=12; By similarityAdd
BLAST
Topological domaini989 – 102638Extracellular By similarityAdd
BLAST
Transmembranei1027 – 104721Helical; Name=13; By similarityAdd
BLAST
Topological domaini1048 – 109043Cytoplasmic By similarityAdd
BLAST
Transmembranei1091 – 111121Helical; Name=14; By similarityAdd
BLAST
Topological domaini1112 – 11121Extracellular By similarity
Transmembranei1113 – 113321Helical; Name=15; By similarityAdd
BLAST
Topological domaini1134 – 120471Cytoplasmic By similarityAdd
BLAST
Transmembranei1205 – 122521Helical; Name=16; By similarityAdd
BLAST
Topological domaini1226 – 12272Extracellular By similarity
Transmembranei1228 – 124821Helical; Name=17; By similarityAdd
BLAST
Topological domaini1249 – 1532284Cytoplasmic By similarityAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: RGD
  2. cytoplasm Source: RGD
  3. integral component of plasma membrane Source: RGD
  4. membrane Source: RGD
  5. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi983 – 9831L → M: No effect on estradiol glucuronide transport. 1 Publication
Mutagenesisi1090 – 10901Q → E: 7.6-fold increase of the estradiol glucuronide transport; when associated with T-1243. Increases doxorubicin inhibition. 1 Publication
Mutagenesisi1101 – 11011S → N: 50% increase of estradiol glucuronide transport. 1 Publication
Mutagenesisi1106 – 11061V → C: No effect on estradiol glucuronide transport. 1 Publication
Mutagenesisi1243 – 12431A → T: 7.6-fold increase of the estradiol glucuronide transport; when associated with E-1090. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15321532Multidrug resistance-associated protein 1PRO_0000093354Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi19 – 191N-linked (GlcNAc...) Reviewed prediction
Modified residuei504 – 5041N6-succinyllysine By similarity
Modified residuei879 – 8791Phosphoserine By similarity
Modified residuei883 – 8831Phosphoserine By similarity
Modified residuei916 – 9161Phosphoserine By similarity
Modified residuei931 – 9311Phosphoserine By similarity

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8CG09.
PRIDEiQ8CG09.

PTM databases

PhosphoSiteiQ8CG09.

Expressioni

Tissue specificityi

Skeletal muscle, brain, heart, spleen, lung and kidney.1 Publication

Gene expression databases

GenevestigatoriQ8CG09.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000045518.

Structurei

3D structure databases

ProteinModelPortaliQ8CG09.
SMRiQ8CG09. Positions 643-872.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini326 – 609284ABC transmembrane type-1 1Add
BLAST
Domaini645 – 869225ABC transporter 1Add
BLAST
Domaini975 – 1257283ABC transmembrane type-1 2Add
BLAST
Domaini1294 – 1528235ABC transporter 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
HOVERGENiHBG108314.
KOiK05665.
PhylomeDBiQ8CG09.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR018170. Aldo/ket_reductase_CS.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsiTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8CG09-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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MALSSFCSSD GSDPLWDWNV TWHTSNPDFT KCFQNTVLTW VPCFYLWSCF     50
PLYFLYLSRH DRGYIQMTHL NKAKTALGFF LWIICWADLF YSFWERSQGM 100
LLAPVLLVSP TLLGITMLLA TFLIQFERRK GVQSSGIMLT FWLVALLCAL 150
AILRSKIISA LKKDAQVDMF RDSAFYLYFT LVFIQLVLSC FSDSSPLFSE 200
TVRDPNPCPE SSASFLSRIT FWWITGMMVQ GYRQPLKSSD LWSLNKEDTS 250
EEVVPVLVNN WKKECVKSRK QPVRIVYAPP KDPTKPKGSS QLDVNEEVEA 300
LIVKSSHKDR DPSLFKVLYK TFGPYFLMSF LYKALHDLMM FAGPEILELI 350
INFVNDREAP DWQGYLYTAL LFVSACLQTL ALHQYFHICF VTGMRIKTAV 400
VGAVYRKALV ITNSARKSST VGEIVNLMSV DAQRFMDLAT YINMIWSAPL 450
QVTLALYFLW LNLGPSVLAG VAVMILMVPF NAVMAMKTKT YQVAHMKSKD 500
NRIKLMNEIL NGIKVLKLYA WELAFQDKVM NIRQEELKVL KKSAYLAAVG 550
TFTWVCTPFL VALSTFAVFV TVDEKNILDA KKAFVSLALF NILRFPLNIL 600
PMVISSIVQA SVSLKRLRIF LSHEELEPDS IERWSIKDGG GMNSITVKNA 650
TFTWARDEPP TLNGITFAIP DGALVAVVGQ VGCGKSSLLS ALLAEMDKVE 700
GHVTLKGSVA YVPQQAWIQN DSLRENILFG RPLQEHCYKA VMEACALLPD 750
LEILPSGDLT EIGEKGVNLS GGQKQRVSLA RAVYCNSDIY LLDDPLSAVD 800
AHVGKHIFEK VVGPMGLLKN KTRILVTHGI SYLPQVDVII VMSGGKISEM 850
GSYQELLDRD GAFAEFVRTY ANTEQDLASE DDSKNGVSGL GKESKPVENG 900
ILVTDAVGKP LQRHLSNSSS HSVVTNQQHS STAELQKSGV KEETWKLMEA 950
DKAQTGQVKL SVYWNYMKAI GLCISFLSIF LFLCNHVSAL ASNYWLSLWT 1000
DDRPAVNGTQ ENRNFRLSVY GALGILQGVA VFGYSMAVSI GGIFASRRLH 1050
LDLLQNVLRS PMSFFERTPS GNLVNRFSKE LDTVDSMIPQ VIKMFMGSLF 1100
SVIGAVIIIL LATPIAAVII PPLGLVYFFV QRFYVASSRQ LKRLESVSRS 1150
PVYSHFNETL LGVSVIRAFE EQERFIRQSD LKVDENQKAY YPSIVANRWL 1200
AVRLECVGNC IVLFAALFAV ISRHSLSAGL VGLSVSYSLQ ITAYLNWLVR 1250
MSSEMETNIV AVERLKEYSE TEKEASWQIQ ETAPPSTWPH SGRVEFRDYC 1300
LRYREDLDLV LKHINVTIEG GEKVGIVGRT GAGKSSLTLG LFRINESAEG 1350
EIIIDGINIA KIGLHNLRFK ITIIPQDPVL FSGSLRMNLD PFSQYSDEEV 1400
WMALELAHLK GFVSALPDKL NHECAEGGEN LSVGQRQLVC LARALLRKTK 1450
ILVLDEATAA VDLETDDLIQ STIRTQFEDS TVLTIAHRLN TIMDYTRVIV 1500
LDKGEIRECG APSELLQQRG VFYSMAKDAG LV 1532
Length:1,532
Mass (Da):171,493
Last modified:January 24, 2006 - v2
Checksum:i2E6939F63F5A3F68
GO
Isoform 2 (identifier: Q8CG09-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     912-920: Missing.

Note: No experimental confirmation available.

Show »
Length:1,523
Mass (Da):170,496
Checksum:i15FB4E9C99B7B9AC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei912 – 9209Missing in isoform 2. VSP_017015

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41S → R in AAN86532. 1 Publication
Sequence conflicti4 – 41S → R in AAO44983. 1 Publication
Sequence conflicti480 – 4801F → S in AAO44983. 1 Publication
Sequence conflicti820 – 8201N → S in CAB97204. 1 Publication
Sequence conflicti868 – 8681R → P in CAB97204. 1 Publication
Sequence conflicti916 – 9161S → G in CAB97204. 1 Publication
Sequence conflicti1122 – 11221P → T in CAB97204. 1 Publication
Sequence conflicti1382 – 13821S → P in AAN86532. 1 Publication
Sequence conflicti1473 – 14731I → V in AAN86532. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY170916 mRNA. Translation: AAN86532.1.
AF487549 mRNA. Translation: AAO85437.1.
AY174892 mRNA. Translation: AAO44983.1.
AJ277881 mRNA. Translation: CAB97204.1.
X96394 mRNA. Translation: CAA65258.1.
RefSeqiNP_071617.2. NM_022281.2. [Q8CG09-1]
UniGeneiRn.10495.

Genome annotation databases

GeneIDi24565.
KEGGirno:24565.
UCSCiRGD:3112. rat. [Q8CG09-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY170916 mRNA. Translation: AAN86532.1 .
AF487549 mRNA. Translation: AAO85437.1 .
AY174892 mRNA. Translation: AAO44983.1 .
AJ277881 mRNA. Translation: CAB97204.1 .
X96394 mRNA. Translation: CAA65258.1 .
RefSeqi NP_071617.2. NM_022281.2. [Q8CG09-1 ]
UniGenei Rn.10495.

3D structure databases

ProteinModelPortali Q8CG09.
SMRi Q8CG09. Positions 643-872.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000045518.

Chemistry

ChEMBLi CHEMBL5680.

PTM databases

PhosphoSitei Q8CG09.

Proteomic databases

PaxDbi Q8CG09.
PRIDEi Q8CG09.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24565.
KEGGi rno:24565.
UCSCi RGD:3112. rat. [Q8CG09-1 ]

Organism-specific databases

CTDi 4363.
RGDi 3112. Abcc1.

Phylogenomic databases

eggNOGi COG1132.
HOVERGENi HBG108314.
KOi K05665.
PhylomeDBi Q8CG09.

Miscellaneous databases

NextBioi 603696.
PROi Q8CG09.

Gene expression databases

Genevestigatori Q8CG09.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR018170. Aldo/ket_reductase_CS.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsi TIGR00957. MRP_assoc_pro. 1 hit.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the rat multidrug resistance-associated protein 1."
    Yang Z., Li C.S.W., Shen D.D., Ho R.J.Y.
    AAPS PharmSci 4:E15-E15(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Molecular cloning and pharmacological characterization of rat multidrug resistance protein 1 (mrp1)."
    Nunoya K., Grant C.E., Zhang D.-W., Cole S.P.C., Deeley R.G.
    Drug Metab. Dispos. 31:1016-1026(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, MUTAGENESIS OF LEU-983; GLN-1090; SER-1101; VAL-1106 AND ALA-1243.
    Strain: Sprague-Dawley.
    Tissue: Skeletal muscle.
  3. Yabuuchi H., Takayanagi S., Ishikawa T.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
    Tissue: Spleen.
  4. Takayanagi S., Ishikawa T.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Sprague-Dawley.
    Tissue: Spleen.
  5. "The multidrug resistance protein MRP1 mediates the release of glutathione disulfide from rat astrocytes during oxidative stress."
    Hirrlinger J., Koenig J., Keppler D., Lindenau J., Schulz J.B., Dringen R.
    J. Neurochem. 76:627-636(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 711-1532 (ISOFORM 1).
  6. "cDNA cloning of the hepatocyte canalicular isoform of the multidrug resistance protein, cMrp, reveals a novel conjugate export pump deficient in hyperbilirubinemic mutant rats."
    Buechler M., Koenig J., Brom M., Kartenbeck J., Spring H., Horie T., Keppler D.
    J. Biol. Chem. 271:15091-15098(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1426-1532 (ISOFORMS 1/2).
  7. "Multidrug resistance protein 1-mediated transport of saquinavir by microglia."
    Dallas S., Ronaldson P.T., Bendayan M., Bendayan R.
    NeuroReport 15:1183-1186(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMRP1_RAT
AccessioniPrimary (citable) accession number: Q8CG09
Secondary accession number(s): Q63346
, Q810E4, Q810G9, Q9JHS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: May 14, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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