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Q8CG03

- PDE5A_MOUSE

UniProt

Q8CG03 - PDE5A_MOUSE

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Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde5a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP (By similarity).By similarity

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei603 – 6031Proton donorBy similarity
Metal bindingi607 – 6071Divalent metal cation 1By similarity
Metal bindingi643 – 6431Divalent metal cation 1By similarity
Metal bindingi644 – 6441Divalent metal cation 1By similarity
Metal bindingi644 – 6441Divalent metal cation 2By similarity
Metal bindingi754 – 7541Divalent metal cation 1By similarity
Binding sitei807 – 8071cGMPBy similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: MGI
  3. cGMP binding Source: MGI
  4. cyclic-nucleotide phosphodiesterase activity Source: MGI
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cGMP catabolic process Source: MGI
  2. cGMP metabolic process Source: MGI
  3. negative regulation of cardiac muscle contraction Source: BHF-UCL
  4. negative regulation of T cell proliferation Source: MGI
  5. positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
  6. positive regulation of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
  7. positive regulation of MAP kinase activity Source: BHF-UCL
  8. positive regulation of oocyte development Source: MGI
  9. relaxation of cardiac muscle Source: BHF-UCL
  10. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_229620. cGMP effects.
UniPathwayiUPA00763; UER00748.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase (EC:3.1.4.35)
Alternative name(s):
cGMP-binding cGMP-specific phosphodiesterase
Short name:
CGB-PDE
Gene namesi
Name:Pde5a
Synonyms:Pde5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:2651499. Pde5a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000198824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei92 – 921PhosphoserineSequence Analysis

Post-translational modificationi

Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Phosphorylation by PRKG1 leads to its activation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CG03.
PaxDbiQ8CG03.
PRIDEiQ8CG03.

PTM databases

PhosphoSiteiQ8CG03.

Expressioni

Gene expression databases

BgeeiQ8CG03.
CleanExiMM_PDE5A.
GenevestigatoriQ8CG03.

Interactioni

Protein-protein interaction databases

IntActiQ8CG03. 1 interaction.
MINTiMINT-4106875.

Structurei

Secondary structure

1
865
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi158 – 1669Combined sources
Turni167 – 1715Combined sources
Beta strandi173 – 18311Combined sources
Turni184 – 1863Combined sources
Beta strandi187 – 19711Combined sources
Turni204 – 2063Combined sources
Beta strandi207 – 2093Combined sources
Beta strandi212 – 2143Combined sources
Turni215 – 2173Combined sources
Helixi219 – 2268Combined sources
Beta strandi230 – 2334Combined sources
Beta strandi238 – 2403Combined sources
Helixi245 – 2495Combined sources
Beta strandi256 – 2638Combined sources
Turni264 – 2663Combined sources
Beta strandi267 – 2759Combined sources
Helixi287 – 30014Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K31NMR-A154-320[»]
ProteinModelPortaliQ8CG03.
SMRiQ8CG03. Positions 89-850.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CG03.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 304151GAF 1Add
BLAST
Domaini336 – 493158GAF 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni578 – 843266CatalyticBy similarityAdd
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.

Sequence similaritiesi

Contains 2 GAF domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000007068.
HOVERGENiHBG101207.
InParanoidiQ8CG03.
KOiK13762.
OMAiREHDANK.
OrthoDBiEOG7RRF69.
TreeFamiTF316499.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 2 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CG03-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERAGPNSVR SQQQRDPDWV EAWLDDHRDF TFSYFIRKAT RDMVNAWFSE
60 70 80 90 100
RVHNIPVCKE GIRAHTESCS CSLQQSPHAD NTTPGAPARK ISASEFDRPL
110 120 130 140 150
RPIVVKDSEG TVSFLSDSGK KEQMPLTPPR FDSDEGDQCS RLLELVKDIS
160 170 180 190 200
SHLDVTALCH KIFLHIHGLI SADRYSLFLV CEDSSKDKFL ISRLFDVAEG
210 220 230 240 250
STLEEASNNC IRLEWNKGIV GHVAAFGEPL NIKDAYEDPR FNAEVDQITG
260 270 280 290 300
YKTQSILCMP IKNHREEVVG VAQAINKKSG NGGTFTEKDE KDFAAYLAFC
310 320 330 340 350
GIVLHNAQLY ETSLLENKRN QVLLDLASLI FEEQQSLEVI LKKIAATIIS
360 370 380 390 400
FMQVQKCTIF IVDEDCPDSF SRVFHMECEE VGKPSDPLTR EQDANKINYM
410 420 430 440 450
YAQYVKNTME PLNIPDVTKD KRFPWTNENM GHVNTPCIGS LLCTPIKNGK
460 470 480 490 500
KNKVIGVCQL VNKMEENTGK IKAFNQNDEQ FLEAFVIFCG LGIQNTQMYE
510 520 530 540 550
AVERAMAKQM VTLEVLSYHA SAAEEETREL QALSAAVVPS AQTLKITDFS
560 570 580 590 600
FSDFELSDLE TALCTIRMFT DLNLVQNFQM KHEVLCRWIL SVKKNYRKNV
610 620 630 640 650
AYHNWRHAFN TAQCMFAALK AGKIQNKLTD LETLALLIAA LSHDLDHRGV
660 670 680 690 700
NNSYIQRSEH PLAQLYCHSI MEHHHFDQCL MILNSPGNQI LSGLSIDEYK
710 720 730 740 750
TTLKIIKQAI LATDLALYIK RRGEFFELIR KNQFSFEDPL QKELFLAMLM
760 770 780 790 800
TACDLSAITK PWPIQQRIAE LVAAEFFDQG DRERKELNME PADLMNREKK
810 820 830 840 850
NKIPSMQVGF IDAICLQLYE ALTHVSEDCL PLLDGCRKNR QKWQALAEQQ
860
EKMLLNGESS QGKRD
Length:865
Mass (Da):98,407
Last modified:July 27, 2011 - v2
Checksum:iBADD21CC6B62045E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761S → T in AAN17330. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF541937 mRNA. Translation: AAN17330.1.
CH466532 Genomic DNA. Translation: EDL12317.1.
BC119137 mRNA. Translation: AAI19138.1.
BC120486 mRNA. Translation: AAI20487.1.
CCDSiCCDS17812.1.
RefSeqiNP_700471.2. NM_153422.2.
UniGeneiMm.134911.

Genome annotation databases

EnsembliENSMUST00000066728; ENSMUSP00000069011; ENSMUSG00000053965.
GeneIDi242202.
KEGGimmu:242202.
UCSCiuc008ret.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF541937 mRNA. Translation: AAN17330.1 .
CH466532 Genomic DNA. Translation: EDL12317.1 .
BC119137 mRNA. Translation: AAI19138.1 .
BC120486 mRNA. Translation: AAI20487.1 .
CCDSi CCDS17812.1.
RefSeqi NP_700471.2. NM_153422.2.
UniGenei Mm.134911.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2K31 NMR - A 154-320 [» ]
ProteinModelPortali Q8CG03.
SMRi Q8CG03. Positions 89-850.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8CG03. 1 interaction.
MINTi MINT-4106875.

PTM databases

PhosphoSitei Q8CG03.

Proteomic databases

MaxQBi Q8CG03.
PaxDbi Q8CG03.
PRIDEi Q8CG03.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000066728 ; ENSMUSP00000069011 ; ENSMUSG00000053965 .
GeneIDi 242202.
KEGGi mmu:242202.
UCSCi uc008ret.2. mouse.

Organism-specific databases

CTDi 8654.
MGIi MGI:2651499. Pde5a.

Phylogenomic databases

eggNOGi NOG270709.
GeneTreei ENSGT00760000119066.
HOGENOMi HOG000007068.
HOVERGENi HBG101207.
InParanoidi Q8CG03.
KOi K13762.
OMAi REHDANK.
OrthoDBi EOG7RRF69.
TreeFami TF316499.

Enzyme and pathway databases

UniPathwayi UPA00763 ; UER00748 .
Reactomei REACT_229620. cGMP effects.

Miscellaneous databases

ChiTaRSi Pde5a. mouse.
EvolutionaryTracei Q8CG03.
NextBioi 385258.
PROi Q8CG03.
SOURCEi Search...

Gene expression databases

Bgeei Q8CG03.
CleanExi MM_PDE5A.
Genevestigatori Q8CG03.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 2 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Burns F., Sonnenburg W.K., Rybalkin S.D., Beavo J.A.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiPDE5A_MOUSE
AccessioniPrimary (citable) accession number: Q8CG03
Secondary accession number(s): Q0VBW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3