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Q8CG03

- PDE5A_MOUSE

UniProt

Q8CG03 - PDE5A_MOUSE

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Protein
cGMP-specific 3',5'-cyclic phosphodiesterase
Gene
Pde5a, Pde5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP By similarity.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei603 – 6031Proton donor By similarity
Metal bindingi607 – 6071Divalent metal cation 1 By similarity
Metal bindingi643 – 6431Divalent metal cation 1 By similarity
Metal bindingi644 – 6441Divalent metal cation 1 By similarity
Metal bindingi644 – 6441Divalent metal cation 2 By similarity
Metal bindingi754 – 7541Divalent metal cation 1 By similarity
Binding sitei807 – 8071cGMP By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: MGI
  3. cGMP binding Source: MGI
  4. cyclic-nucleotide phosphodiesterase activity Source: MGI
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cGMP catabolic process Source: MGI
  2. cGMP metabolic process Source: MGI
  3. negative regulation of T cell proliferation Source: MGI
  4. negative regulation of cardiac muscle contraction Source: BHF-UCL
  5. positive regulation of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
  6. positive regulation of MAP kinase activity Source: BHF-UCL
  7. positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
  8. positive regulation of oocyte development Source: MGI
  9. relaxation of cardiac muscle Source: BHF-UCL
  10. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00763; UER00748.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase (EC:3.1.4.35)
Alternative name(s):
cGMP-binding cGMP-specific phosphodiesterase
Short name:
CGB-PDE
Gene namesi
Name:Pde5a
Synonyms:Pde5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:2651499. Pde5a.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 865865cGMP-specific 3',5'-cyclic phosphodiesterase
PRO_0000198824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei92 – 921Phosphoserine Reviewed prediction

Post-translational modificationi

Phosphorylation is regulated by binding of cGMP to the two allosteric sites By similarity. Phosphorylation by PRKG1 leads to its activation By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CG03.
PaxDbiQ8CG03.
PRIDEiQ8CG03.

PTM databases

PhosphoSiteiQ8CG03.

Expressioni

Gene expression databases

BgeeiQ8CG03.
CleanExiMM_PDE5A.
GenevestigatoriQ8CG03.

Interactioni

Protein-protein interaction databases

IntActiQ8CG03. 1 interaction.
MINTiMINT-4106875.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi158 – 1669
Turni167 – 1715
Beta strandi173 – 18311
Turni184 – 1863
Beta strandi187 – 19711
Turni204 – 2063
Beta strandi207 – 2093
Beta strandi212 – 2143
Turni215 – 2173
Helixi219 – 2268
Beta strandi230 – 2334
Beta strandi238 – 2403
Helixi245 – 2495
Beta strandi256 – 2638
Turni264 – 2663
Beta strandi267 – 2759
Helixi287 – 30014

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K31NMR-A154-320[»]
ProteinModelPortaliQ8CG03.
SMRiQ8CG03. Positions 89-850.

Miscellaneous databases

EvolutionaryTraceiQ8CG03.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 304151GAF 1
Add
BLAST
Domaini336 – 493158GAF 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni578 – 843266Catalytic By similarity
Add
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.

Sequence similaritiesi

Contains 2 GAF domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
GeneTreeiENSGT00750000117528.
HOGENOMiHOG000007068.
HOVERGENiHBG101207.
InParanoidiQ0VBW0.
KOiK13762.
OMAiREHDANK.
OrthoDBiEOG7RRF69.
TreeFamiTF316499.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 2 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CG03-1 [UniParc]FASTAAdd to Basket

« Hide

MERAGPNSVR SQQQRDPDWV EAWLDDHRDF TFSYFIRKAT RDMVNAWFSE    50
RVHNIPVCKE GIRAHTESCS CSLQQSPHAD NTTPGAPARK ISASEFDRPL 100
RPIVVKDSEG TVSFLSDSGK KEQMPLTPPR FDSDEGDQCS RLLELVKDIS 150
SHLDVTALCH KIFLHIHGLI SADRYSLFLV CEDSSKDKFL ISRLFDVAEG 200
STLEEASNNC IRLEWNKGIV GHVAAFGEPL NIKDAYEDPR FNAEVDQITG 250
YKTQSILCMP IKNHREEVVG VAQAINKKSG NGGTFTEKDE KDFAAYLAFC 300
GIVLHNAQLY ETSLLENKRN QVLLDLASLI FEEQQSLEVI LKKIAATIIS 350
FMQVQKCTIF IVDEDCPDSF SRVFHMECEE VGKPSDPLTR EQDANKINYM 400
YAQYVKNTME PLNIPDVTKD KRFPWTNENM GHVNTPCIGS LLCTPIKNGK 450
KNKVIGVCQL VNKMEENTGK IKAFNQNDEQ FLEAFVIFCG LGIQNTQMYE 500
AVERAMAKQM VTLEVLSYHA SAAEEETREL QALSAAVVPS AQTLKITDFS 550
FSDFELSDLE TALCTIRMFT DLNLVQNFQM KHEVLCRWIL SVKKNYRKNV 600
AYHNWRHAFN TAQCMFAALK AGKIQNKLTD LETLALLIAA LSHDLDHRGV 650
NNSYIQRSEH PLAQLYCHSI MEHHHFDQCL MILNSPGNQI LSGLSIDEYK 700
TTLKIIKQAI LATDLALYIK RRGEFFELIR KNQFSFEDPL QKELFLAMLM 750
TACDLSAITK PWPIQQRIAE LVAAEFFDQG DRERKELNME PADLMNREKK 800
NKIPSMQVGF IDAICLQLYE ALTHVSEDCL PLLDGCRKNR QKWQALAEQQ 850
EKMLLNGESS QGKRD 865
Length:865
Mass (Da):98,407
Last modified:July 27, 2011 - v2
Checksum:iBADD21CC6B62045E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761S → T in AAN17330. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF541937 mRNA. Translation: AAN17330.1.
CH466532 Genomic DNA. Translation: EDL12317.1.
BC119137 mRNA. Translation: AAI19138.1.
BC120486 mRNA. Translation: AAI20487.1.
CCDSiCCDS17812.1.
RefSeqiNP_700471.2. NM_153422.2.
UniGeneiMm.134911.

Genome annotation databases

EnsembliENSMUST00000066728; ENSMUSP00000069011; ENSMUSG00000053965.
GeneIDi242202.
KEGGimmu:242202.
UCSCiuc008ret.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF541937 mRNA. Translation: AAN17330.1 .
CH466532 Genomic DNA. Translation: EDL12317.1 .
BC119137 mRNA. Translation: AAI19138.1 .
BC120486 mRNA. Translation: AAI20487.1 .
CCDSi CCDS17812.1.
RefSeqi NP_700471.2. NM_153422.2.
UniGenei Mm.134911.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2K31 NMR - A 154-320 [» ]
ProteinModelPortali Q8CG03.
SMRi Q8CG03. Positions 89-850.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8CG03. 1 interaction.
MINTi MINT-4106875.

PTM databases

PhosphoSitei Q8CG03.

Proteomic databases

MaxQBi Q8CG03.
PaxDbi Q8CG03.
PRIDEi Q8CG03.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000066728 ; ENSMUSP00000069011 ; ENSMUSG00000053965 .
GeneIDi 242202.
KEGGi mmu:242202.
UCSCi uc008ret.2. mouse.

Organism-specific databases

CTDi 8654.
MGIi MGI:2651499. Pde5a.

Phylogenomic databases

eggNOGi NOG270709.
GeneTreei ENSGT00750000117528.
HOGENOMi HOG000007068.
HOVERGENi HBG101207.
InParanoidi Q0VBW0.
KOi K13762.
OMAi REHDANK.
OrthoDBi EOG7RRF69.
TreeFami TF316499.

Enzyme and pathway databases

UniPathwayi UPA00763 ; UER00748 .

Miscellaneous databases

ChiTaRSi PDE5A. mouse.
EvolutionaryTracei Q8CG03.
NextBioi 385258.
PROi Q8CG03.
SOURCEi Search...

Gene expression databases

Bgeei Q8CG03.
CleanExi MM_PDE5A.
Genevestigatori Q8CG03.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 2 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 2 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Burns F., Sonnenburg W.K., Rybalkin S.D., Beavo J.A.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiPDE5A_MOUSE
AccessioniPrimary (citable) accession number: Q8CG03
Secondary accession number(s): Q0VBW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi