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Q8CG03

- PDE5A_MOUSE

UniProt

Q8CG03 - PDE5A_MOUSE

Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde5a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP By similarity.By similarity

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei603 – 6031Proton donorBy similarity
    Metal bindingi607 – 6071Divalent metal cation 1By similarity
    Metal bindingi643 – 6431Divalent metal cation 1By similarity
    Metal bindingi644 – 6441Divalent metal cation 1By similarity
    Metal bindingi644 – 6441Divalent metal cation 2By similarity
    Metal bindingi754 – 7541Divalent metal cation 1By similarity
    Binding sitei807 – 8071cGMPBy similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: MGI
    3. cGMP binding Source: MGI
    4. cyclic-nucleotide phosphodiesterase activity Source: MGI
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cGMP catabolic process Source: MGI
    2. cGMP metabolic process Source: MGI
    3. negative regulation of cardiac muscle contraction Source: BHF-UCL
    4. negative regulation of T cell proliferation Source: MGI
    5. positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
    6. positive regulation of G-protein coupled receptor protein signaling pathway Source: BHF-UCL
    7. positive regulation of MAP kinase activity Source: BHF-UCL
    8. positive regulation of oocyte development Source: MGI
    9. relaxation of cardiac muscle Source: BHF-UCL
    10. signal transduction Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00763; UER00748.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesterase (EC:3.1.4.35)
    Alternative name(s):
    cGMP-binding cGMP-specific phosphodiesterase
    Short name:
    CGB-PDE
    Gene namesi
    Name:Pde5a
    Synonyms:Pde5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:2651499. Pde5a.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 865865cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000198824Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei92 – 921PhosphoserineSequence Analysis

    Post-translational modificationi

    Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Phosphorylation by PRKG1 leads to its activation.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8CG03.
    PaxDbiQ8CG03.
    PRIDEiQ8CG03.

    PTM databases

    PhosphoSiteiQ8CG03.

    Expressioni

    Gene expression databases

    BgeeiQ8CG03.
    CleanExiMM_PDE5A.
    GenevestigatoriQ8CG03.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8CG03. 1 interaction.
    MINTiMINT-4106875.

    Structurei

    Secondary structure

    1
    865
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi158 – 1669
    Turni167 – 1715
    Beta strandi173 – 18311
    Turni184 – 1863
    Beta strandi187 – 19711
    Turni204 – 2063
    Beta strandi207 – 2093
    Beta strandi212 – 2143
    Turni215 – 2173
    Helixi219 – 2268
    Beta strandi230 – 2334
    Beta strandi238 – 2403
    Helixi245 – 2495
    Beta strandi256 – 2638
    Turni264 – 2663
    Beta strandi267 – 2759
    Helixi287 – 30014

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2K31NMR-A154-320[»]
    ProteinModelPortaliQ8CG03.
    SMRiQ8CG03. Positions 89-850.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8CG03.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini154 – 304151GAF 1Add
    BLAST
    Domaini336 – 493158GAF 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni578 – 843266CatalyticBy similarityAdd
    BLAST

    Domaini

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270709.
    GeneTreeiENSGT00750000117528.
    HOGENOMiHOG000007068.
    HOVERGENiHBG101207.
    InParanoidiQ0VBW0.
    KOiK13762.
    OMAiREHDANK.
    OrthoDBiEOG7RRF69.
    TreeFamiTF316499.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 2 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 2 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CG03-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERAGPNSVR SQQQRDPDWV EAWLDDHRDF TFSYFIRKAT RDMVNAWFSE    50
    RVHNIPVCKE GIRAHTESCS CSLQQSPHAD NTTPGAPARK ISASEFDRPL 100
    RPIVVKDSEG TVSFLSDSGK KEQMPLTPPR FDSDEGDQCS RLLELVKDIS 150
    SHLDVTALCH KIFLHIHGLI SADRYSLFLV CEDSSKDKFL ISRLFDVAEG 200
    STLEEASNNC IRLEWNKGIV GHVAAFGEPL NIKDAYEDPR FNAEVDQITG 250
    YKTQSILCMP IKNHREEVVG VAQAINKKSG NGGTFTEKDE KDFAAYLAFC 300
    GIVLHNAQLY ETSLLENKRN QVLLDLASLI FEEQQSLEVI LKKIAATIIS 350
    FMQVQKCTIF IVDEDCPDSF SRVFHMECEE VGKPSDPLTR EQDANKINYM 400
    YAQYVKNTME PLNIPDVTKD KRFPWTNENM GHVNTPCIGS LLCTPIKNGK 450
    KNKVIGVCQL VNKMEENTGK IKAFNQNDEQ FLEAFVIFCG LGIQNTQMYE 500
    AVERAMAKQM VTLEVLSYHA SAAEEETREL QALSAAVVPS AQTLKITDFS 550
    FSDFELSDLE TALCTIRMFT DLNLVQNFQM KHEVLCRWIL SVKKNYRKNV 600
    AYHNWRHAFN TAQCMFAALK AGKIQNKLTD LETLALLIAA LSHDLDHRGV 650
    NNSYIQRSEH PLAQLYCHSI MEHHHFDQCL MILNSPGNQI LSGLSIDEYK 700
    TTLKIIKQAI LATDLALYIK RRGEFFELIR KNQFSFEDPL QKELFLAMLM 750
    TACDLSAITK PWPIQQRIAE LVAAEFFDQG DRERKELNME PADLMNREKK 800
    NKIPSMQVGF IDAICLQLYE ALTHVSEDCL PLLDGCRKNR QKWQALAEQQ 850
    EKMLLNGESS QGKRD 865
    Length:865
    Mass (Da):98,407
    Last modified:July 27, 2011 - v2
    Checksum:iBADD21CC6B62045E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti176 – 1761S → T in AAN17330. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF541937 mRNA. Translation: AAN17330.1.
    CH466532 Genomic DNA. Translation: EDL12317.1.
    BC119137 mRNA. Translation: AAI19138.1.
    BC120486 mRNA. Translation: AAI20487.1.
    CCDSiCCDS17812.1.
    RefSeqiNP_700471.2. NM_153422.2.
    UniGeneiMm.134911.

    Genome annotation databases

    EnsembliENSMUST00000066728; ENSMUSP00000069011; ENSMUSG00000053965.
    GeneIDi242202.
    KEGGimmu:242202.
    UCSCiuc008ret.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF541937 mRNA. Translation: AAN17330.1 .
    CH466532 Genomic DNA. Translation: EDL12317.1 .
    BC119137 mRNA. Translation: AAI19138.1 .
    BC120486 mRNA. Translation: AAI20487.1 .
    CCDSi CCDS17812.1.
    RefSeqi NP_700471.2. NM_153422.2.
    UniGenei Mm.134911.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2K31 NMR - A 154-320 [» ]
    ProteinModelPortali Q8CG03.
    SMRi Q8CG03. Positions 89-850.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8CG03. 1 interaction.
    MINTi MINT-4106875.

    PTM databases

    PhosphoSitei Q8CG03.

    Proteomic databases

    MaxQBi Q8CG03.
    PaxDbi Q8CG03.
    PRIDEi Q8CG03.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000066728 ; ENSMUSP00000069011 ; ENSMUSG00000053965 .
    GeneIDi 242202.
    KEGGi mmu:242202.
    UCSCi uc008ret.2. mouse.

    Organism-specific databases

    CTDi 8654.
    MGIi MGI:2651499. Pde5a.

    Phylogenomic databases

    eggNOGi NOG270709.
    GeneTreei ENSGT00750000117528.
    HOGENOMi HOG000007068.
    HOVERGENi HBG101207.
    InParanoidi Q0VBW0.
    KOi K13762.
    OMAi REHDANK.
    OrthoDBi EOG7RRF69.
    TreeFami TF316499.

    Enzyme and pathway databases

    UniPathwayi UPA00763 ; UER00748 .

    Miscellaneous databases

    ChiTaRSi PDE5A. mouse.
    EvolutionaryTracei Q8CG03.
    NextBioi 385258.
    PROi Q8CG03.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8CG03.
    CleanExi MM_PDE5A.
    Genevestigatori Q8CG03.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 2 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 2 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Burns F., Sonnenburg W.K., Rybalkin S.D., Beavo J.A.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiPDE5A_MOUSE
    AccessioniPrimary (citable) accession number: Q8CG03
    Secondary accession number(s): Q0VBW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3