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Q8CFZ5 (S22AC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 12
Alternative name(s):
Renal-specific transporter
Short name=RST
Urate anion exchanger 1
Gene names
Name:Slc22a12
Synonyms:Urat1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for efficient urate re-absorption in the kidney. Regulates blood urate levels. Mediates saturable urate uptake by facilitating the exchange of urate against organic anions or chloride ions. Ref.3

Subunit structure

Interacts with PDZK1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Detected in the luminal membrane of the epithelium of renal proximal tubules. Ref.3

Tissue specificity

Detected in kidney (at protein level). Detected in kidney cortex, in proximal tubules. Ref.1 Ref.3

Post-translational modification

N-glycosylated. Ref.3

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Solute carrier family 22 member 12
PRO_0000307945

Regions

Transmembrane10 – 3021Helical; Potential
Transmembrane146 – 16621Helical; Potential
Transmembrane182 – 20221Helical; Potential
Transmembrane204 – 22421Helical; Potential
Transmembrane232 – 25221Helical; Potential
Transmembrane260 – 28021Helical; Potential
Transmembrane351 – 37121Helical; Potential
Transmembrane378 – 39821Helical; Potential
Transmembrane412 – 43221Helical; Potential
Transmembrane435 – 45521Helical; Potential
Transmembrane466 – 48621Helical; Potential
Transmembrane495 – 51521Helical; Potential

Amino acid modifications

Glycosylation561N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4071F → L in BAA23875. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8CFZ5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: D331583A8D54DBBF

FASTA55360,194
        10         20         30         40         50         60 
MAFPELLDRV GGLGRFQLFQ TVALVTPILW VTTQNMLENF SAAVPHHRCW VPLLDNSTSQ 

        70         80         90        100        110        120 
ASIPGDLGPD VLLAVSIPPG PDQQPHQCLR FRQPQWQLTE SNATATNWSD AATEPCEDGW 

       130        140        150        160        170        180 
VYDHSTFRST IVTTWDLVCN SQALRPMAQS IFLAGILVGA AVCGHASDRF GRRRVLTWSY 

       190        200        210        220        230        240 
LLVSVSGTAA AFMPTFPLYC LFRFLLASAV AGVMMNTASL LMEWTSAQGS PLVMTLNALG 

       250        260        270        280        290        300 
FSFGQVLTGS VAYGVRSWRM LQLAVSAPFF LFFVYSWWLP ESARWLITVG KLDQGLQELQ 

       310        320        330        340        350        360 
RVAAVNRRKA EGDTLTMEVL RSAMEEEPSR DKAGASLGTL LHTPGLRHRT IISMLCWFAF 

       370        380        390        400        410        420 
GFTFYGLALD LQALGSNIFL LQALIGIVDF PVKTGSLLLI SRLGRRFCQV SFLVLPGLCI 

       430        440        450        460        470        480 
LSNILVPHGM GVLRSALAVL GLGCLGGAFT CITIFSSELF PTVIRMTAVG LCQVAARGGA 

       490        500        510        520        530        540 
MLGPLVRLLG VYGSWMPLLV YGVVPVLSGL AALLLPETKN LPLPDTIQDI QKQSVKKVTH 

       550 
DTPDGSILMS TRL 

« Hide

References

« Hide 'large scale' references
[1]"Kidney-specific expression of a novel mouse organic cation transporter-like protein."
Mori K., Ogawa Y., Ebihara K., Aoki T., Tamura N., Sugawara A., Kuwahara T., Ozaki S., Mukoyama M., Tashiro K., Tanaka I., Nakao K.
FEBS Lett. 417:371-374(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Kidney.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[3]"Function and localization of urate transporter 1 in mouse kidney."
Hosoyamada M., Ichida K., Enomoto A., Hosoya T., Endou H.
J. Am. Soc. Nephrol. 15:261-268(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GLYCOSYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB005451 mRNA. Translation: BAA23875.1.
BC035927 mRNA. Translation: AAH35927.1.
CCDSCCDS29505.1.
RefSeqNP_033229.3. NM_009203.3.
UniGeneMm.391146.

3D structure databases

ProteinModelPortalQ8CFZ5.
SMRQ8CFZ5. Positions 153-536.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8CFZ5. 2 interactions.
MINTMINT-4104972.

Protein family/group databases

TCDB2.A.1.19.14. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteQ8CFZ5.

Proteomic databases

MaxQBQ8CFZ5.
PaxDbQ8CFZ5.
PRIDEQ8CFZ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000113451; ENSMUSP00000109078; ENSMUSG00000061742.
GeneID20521.
KEGGmmu:20521.
UCSCuc012bho.1. mouse.

Organism-specific databases

CTD116085.
MGIMGI:1195269. Slc22a12.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00750000117657.
HOGENOMHOG000234569.
HOVERGENHBG108433.
InParanoidQ8CFZ5.
KOK08208.
OrthoDBEOG7NW68T.
PhylomeDBQ8CFZ5.
TreeFamTF315847.

Gene expression databases

BgeeQ8CFZ5.
GenevestigatorQ8CFZ5.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298747.
PROQ8CFZ5.
SOURCESearch...

Entry information

Entry nameS22AC_MOUSE
AccessionPrimary (citable) accession number: Q8CFZ5
Secondary accession number(s): O54778
Entry history
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot